Standard name
Description Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.2 0 0 0 0 0 0.05 0.05 0 0 0 0.19 0.21 0.16 0.11 0.08 0.12 0.05 0.05 0.09 0.05 0.05 0.06
Bud 0 0 0 0 0 0 0.06 0.06 0.1 0.05 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.14 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0 0 0 0 0 0 0 0 0 0 0.16 0.13 0.12 0.05 0 0 0 0 0 0 0 0
Endosome 0.18 0 0.18 0.15 0.16 0.11 0.16 0.18 0.08 0.05 0 0.11 0.14 0.14 0.13 0.27 0.32 0.25 0.17 0.19 0.13 0.14 0.12 0.1
Golgi 0.62 0.27 0.49 0.31 0.33 0.08 0.08 0.06 0.06 0 0 0 0.54 0.57 0.64 0.35 0.38 0.54 0.73 0.56 0.69 0.7 0.73 0.76
Mitochondria 0.41 0.82 0.56 0.72 0.64 0.8 0.73 0.69 0.83 0.88 0.88 0.73 0.19 0.19 0.07 0.11 0.08 0.1 0 0.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0.05 0 0.08 0.08 0.08 0.06 0 0.11 0 0 0 0 0 0 0 0 0.06 0.05 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.11 0.06 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 43 31 9 10 13 14 15 40 30 14 21 27 38 43 38 22 47 42 19 8 32 15 28 33
Bud 11 6 13 11 15 11 45 50 59 35 54 74 4 4 3 9 18 12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 3 6 7 8 6 0 0 0 0 1 0
Cell Periphery 0 1 0 1 2 1 4 3 8 1 4 4 4 4 1 1 2 1 0 0 0 0 0 0
Cytoplasm 3 5 7 10 11 8 10 13 6 10 11 8 13 13 11 30 62 26 0 1 0 0 0 2
Endoplasmic Reticulum 10 16 9 3 7 22 20 16 15 11 10 14 32 26 28 11 12 13 0 0 0 1 3 2
Endosome 64 0 62 39 69 56 120 151 44 37 22 76 28 28 31 56 183 87 67 34 47 43 66 60
Golgi 224 42 168 81 142 39 55 53 35 15 21 26 108 116 150 74 215 186 296 99 253 221 398 454
Mitochondria 149 130 194 187 279 397 534 590 474 632 456 510 39 39 17 23 46 34 6 16 7 13 16 13
Nucleus 0 2 0 0 2 1 0 3 0 0 1 0 0 0 0 0 3 2 0 1 0 0 0 0
Nuclear Periphery 0 0 5 1 1 3 5 8 3 9 3 3 2 3 0 1 1 1 0 0 0 0 1 0
Nucleolus 3 0 4 3 7 6 32 49 14 26 11 14 2 2 3 0 11 5 0 0 0 0 0 0
Peroxisomes 3 0 9 15 22 17 57 65 43 42 22 79 2 3 0 1 24 6 12 4 22 17 23 27
SpindlePole 4 0 3 1 7 5 16 28 23 21 14 30 6 6 7 15 50 16 0 1 0 1 1 0
Vac/Vac Membrane 24 7 5 3 10 12 13 11 3 5 3 15 20 20 27 13 52 30 2 7 1 0 1 2
Unique Cell Count 361 158 344 259 435 497 733 854 570 717 520 694 201 203 236 209 566 345 407 178 368 316 545 601
Labelled Cell Count 538 240 488 365 587 593 927 1083 763 865 661 886 298 307 316 256 727 461 407 178 368 316 545 601


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 8.1 10.7 10.0 9.6 8.4 8.1 8.1 7.8 7.0 7.5 7.5 9.9 9.9 10.9 8.1 9.2 11.0 11.8 12.1 12.5
Std Deviation (1e-4) 1.4 1.4 1.8 1.5 1.6 1.5 1.5 1.7 1.7 1.5 1.7 1.6 1.4 1.4 1.2 1.4 1.7 2.1 1.5 1.7 1.8
Intensity Change (Log2) -0.09 -0.15 -0.34 -0.39 -0.4 -0.45 -0.61 -0.52 -0.5 -0.1 -0.1 0.03 -0.4 -0.22 0.05 0.15 0.18 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 0.3 0.2 -0.6 1.6 1.9 -0.7 1.1 1.1 6.5 7.2 5.8 3.9 3.5 4.8
Bud 0.3 -0.2 -1.3 1.6 1.5 3.6 0.8 3.6 3.8 -1.2 -1.2 -1.8 0.3 -0.5 -0.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.5 -0.5 -0.8 -0.6 0 -0.8 0.1 0 2.7 2.6 1.8 5.6 4.9 3.4
Endoplasmic Reticulum -1.3 -1.0 1.4 0.1 -0.8 0 -1.2 -0.7 -0.6 5.7 4.7 4.5 1.6 -0.5 0.9
Endosome -1.0 -0.8 -2.8 -0.7 -0.1 -4.7 -6.7 -6.7 -3.2 -1.2 -1.3 -1.6 2.4 4.7 2.3
Golgi -4.3 -4.6 -13.6 -15.6 -17.2 -15.0 -18.9 -15.6 -17.5 1.1 1.9 3.5 -3.1 -3.2 1.3
Mitochondria 4.0 2.2 7.3 5.4 4.2 8.8 11.7 10.4 5.5 -8.4 -8.5 -12.1 -10.6 -16.0 -13.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.7 3.5 1.4 2.3 1.0 1.0 0 0 0 0 0.9 0
Peroxisomes 2.0 1.7 0.7 3.3 3.2 3.1 2.3 1.2 4.8 0 0 0 0 1.3 -0.8
SpindlePole 0 0 0 1.5 2.4 2.8 2.1 1.9 3.0 0 0 0 4.1 5.0 3.0
Vacuole 0 0.9 1.0 0.4 0 0 0 0 0.8 4.6 4.5 5.2 3.1 4.7 4.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apl5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available