Standard name
Human Ortholog
Description RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.05 0 0 0 0.05 0.06 0.06 0.08 0.07 0.05 0 0.08 0.14 0.08 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.94 0.95 0.86 0.8 0.69 0.68 0.66 0.67 0.68 0.65 0.97 0.99 0.96 0.95 0.91 0.95 0.93 0.92 0.85 0.87 0.88 0.91
Endoplasmic Reticulum 0 0 0.05 0 0.06 0.06 0.16 0.16 0.1 0.12 0.09 0.1 0 0 0 0 0 0 0 0 0.12 0.08 0.06 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.18 0.13 0.12 0.2 0.19 0.25 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.06 0.08 0.1 0.07 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 4 1 7 6 8 12 14 9 0 0 0 0 2 0 0 1 2 0 0 0
Bud 0 0 1 0 1 1 3 8 4 2 6 3 2 2 0 1 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 18 15 7 24 14 18 32 32 46 40 51 18 17 8 17 27 18 0 0 2 0 0 1
Cytoplasm 120 742 420 251 441 415 495 551 446 506 420 432 235 337 264 197 177 217 131 697 382 167 328 259
Endoplasmic Reticulum 1 25 23 8 29 32 113 129 69 90 54 69 4 6 6 5 7 7 3 27 52 14 20 11
Endosome 0 1 1 0 1 0 1 1 1 1 0 1 1 0 1 0 2 1 2 4 0 0 4 0
Golgi 0 2 1 1 0 0 2 2 0 1 1 2 1 0 0 0 1 0 0 2 1 0 3 0
Mitochondria 0 0 0 4 14 96 94 98 136 139 158 121 0 0 0 1 1 1 0 2 2 1 3 2
Nucleus 0 2 1 1 2 1 5 2 2 3 4 0 0 0 1 4 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 6 8 3 0 3 2 7 0 0 0 0 0 0 0 1 0 0 2 0
Nucleolus 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 5 5 26 22 46 63 69 54 26 66 2 0 4 4 7 6 1 6 1 4 6 4
Unique Cell Count 120 764 446 264 511 520 719 808 673 751 622 664 243 342 276 208 194 228 141 755 451 192 374 287
Labelled Cell Count 123 792 468 277 543 589 792 895 767 857 725 762 263 362 284 229 225 252 141 755 451 192 374 287


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 13.8 12.5 11.9 11.0 9.9 9.9 10.4 10.0 10.0 9.8 10.2 13.5 13.0 13.0 11.8 11.9 12.0 11.1 11.4 11.4
Std Deviation (1e-4) 1.5 1.9 2.0 2.0 1.8 1.9 1.9 1.9 2.2 2.1 2.1 2.1 1.5 1.3 1.6 2.2 2.4 2.1 1.2 1.3 1.3
Intensity Change (Log2) -0.07 -0.17 -0.34 -0.33 -0.26 -0.32 -0.32 -0.35 -0.3 0.12 0.06 0.07 -0.08 -0.07 -0.06 -0.16 -0.13 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 1.0 -0.6 -0.9 0.5 1.1 2.1 2.2 3.0 2.4 1.1 -0.3 2.7 5.0 2.6
Cytoplasm 0.5 -4.0 -6.5 -10.2 -10.5 -10.9 -10.7 -10.5 -11.3 1.5 3.1 0.9 0.3 -1.4 0.5
Endoplasmic Reticulum -1.3 0.4 0.7 5.5 5.6 3.0 3.9 2.2 3.1 -2.3 -2.5 -2.0 -1.6 -0.9 -1.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 9.6 8.0 7.7 10.1 9.7 11.5 9.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.5 2.9 4.3 5.0 6.0 4.7 2.9 5.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2246 10.0472 8.507 8.7002 7.0039 9.1108 10.1587 12.6368 11.5673 10.8542 10.4775 11.7355 8.3619 9.4822 8.4417 8.7841 9.3831 9.7182
Actin 0.0261 0.0003 0.0102 0.0122 0.0605 0.0108 0.0187 0.0083 0.0119 0.0207 0.0152 0.0092 0.0098 0.0006 0.036 0.0003 0.001 0.0004
Bud 0.0005 0.0001 0.0002 0.0009 0.0021 0.0003 0.0029 0.0104 0.0041 0.0331 0.0034 0.003 0.0005 0.0001 0.0014 0.0001 0.0002 0.0001
Bud Neck 0.0019 0.0001 0.0004 0.0007 0.0017 0.0011 0.0009 0.0003 0.0008 0.0014 0.0012 0.0021 0.0008 0.0003 0.0004 0.0002 0.0003 0.0005
Bud Periphery 0.0003 0 0.0002 0.0011 0.0019 0.0003 0.0024 0.004 0.0025 0.0163 0.0016 0.0022 0.0009 0.0001 0.0012 0 0 0
Bud Site 0.0025 0.0002 0.0005 0.003 0.0029 0.0004 0.0032 0.0128 0.01 0.0115 0.0011 0.0017 0.006 0.0008 0.0051 0 0.0001 0
Cell Periphery 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0005 0.0005 0.0004 0.001 0.0002 0.0002 0.0003 0.0001 0.0002 0 0 0
Cytoplasm 0.7916 0.969 0.8729 0.8273 0.702 0.911 0.7759 0.9293 0.8697 0.7731 0.8744 0.9291 0.8402 0.9682 0.8162 0.8801 0.9779 0.9344
Cytoplasmic Foci 0.0416 0.0025 0.0101 0.0066 0.0209 0.0082 0.0235 0.0042 0.0117 0.021 0.017 0.0119 0.0276 0.0034 0.0199 0.0055 0.0028 0.0058
Eisosomes 0.0002 0 0.0001 0.0001 0.0017 0.0001 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0402 0.0209 0.0417 0.1007 0.0198 0.023 0.0463 0.0112 0.0378 0.0511 0.0142 0.0144 0.0385 0.0065 0.0201 0.0716 0.0098 0.0287
Endosome 0.0303 0.0029 0.032 0.008 0.0738 0.0166 0.0535 0.0026 0.014 0.0148 0.0248 0.0083 0.0159 0.003 0.0309 0.0245 0.0023 0.0191
Golgi 0.0072 0.0002 0.0075 0.004 0.0145 0.0043 0.014 0.0005 0.0061 0.0022 0.0112 0.0007 0.003 0.0016 0.0207 0.0007 0.0003 0.0007
Lipid Particles 0.0078 0 0.0038 0.0029 0.0382 0.0049 0.0045 0.0001 0.0035 0.0051 0.0012 0.0003 0.0068 0.006 0.0059 0.0004 0 0
Mitochondria 0.0021 0.0001 0.0035 0.0139 0.005 0.0016 0.0144 0.0003 0.005 0.0135 0.0038 0.0008 0.0037 0.0016 0.0101 0.0003 0.0001 0.0011
None 0.0044 0.0007 0.0016 0.0009 0.0113 0.0014 0.0088 0.0026 0.0069 0.0154 0.0026 0.0032 0.0037 0.0006 0.0025 0.0007 0.001 0.0007
Nuclear Periphery 0.0145 0.0016 0.0073 0.0047 0.0115 0.0033 0.011 0.001 0.0033 0.0049 0.0015 0.005 0.0039 0.0025 0.0037 0.0103 0.0017 0.0029
Nucleolus 0.0003 0 0.0001 0.0001 0.0012 0.0001 0.0005 0.0001 0.0003 0.001 0.0181 0.0003 0.0002 0.0003 0.0003 0 0 0
Nucleus 0.0037 0.001 0.0018 0.0024 0.0034 0.0019 0.0046 0.0093 0.003 0.004 0.0036 0.003 0.0016 0.0012 0.0028 0.0027 0.0018 0.0028
Peroxisomes 0.0148 0 0.0021 0.0083 0.0127 0.0047 0.0022 0.0001 0.0016 0.0015 0.0015 0.0003 0.0152 0.0018 0.0093 0 0 0
Punctate Nuclear 0.0037 0.0001 0.0006 0.0003 0.0076 0.0032 0.004 0.0007 0.0043 0.0028 0.0008 0.0022 0.0116 0.0002 0.0112 0.0003 0.0002 0.0001
Vacuole 0.0048 0.0004 0.0027 0.0014 0.0047 0.002 0.006 0.0014 0.0022 0.0039 0.0018 0.0016 0.0075 0.0008 0.0012 0.0017 0.0004 0.0018
Vacuole Periphery 0.0014 0.0001 0.0009 0.0005 0.0023 0.0007 0.002 0.0002 0.0007 0.0013 0.0009 0.0005 0.0024 0.0002 0.0008 0.0007 0.0001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.1002 33.791 44.5767 45.6305 36.5627 43.7759 37.9623 46.6686 35.7289 46.7322
Translational Efficiency 0.6803 0.9455 0.6521 0.6847 0.7869 0.6746 0.7747 0.6077 0.7736 0.6356

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1859 1591 1897 1313 1645 1973 2061 2003 3504 3564 3958 3316

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1029.06 1166.70 1350.80 1413.79 979.32 1108.22 1414.44 1514.30 1005.71 1134.33 1383.94 1474.50
Standard Deviation 149.56 189.58 179.12 236.53 143.79 163.51 204.67 227.92 148.96 178.02 195.45 236.53
Intensity Change Log 2 0.181107 0.392487 0.458241 0.178392 0.530379 0.628799 0.179784 0.461372 0.543928

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000239 0.000319 0.000566 0.002104 0.000166 0.000392 0.000238 0.000491 0.000205 0.000360 0.000395 0.001130
Bud Neck 0.001157 0.000841 0.001667 0.005344 0.000803 0.001234 0.001467 0.001629 0.000991 0.001058 0.001563 0.003100
Bud Site 0.012818 0.013513 0.013023 0.041456 0.011409 0.021829 0.008531 0.019865 0.012156 0.018117 0.010684 0.028414
Cell Periphery 0.000265 0.000248 0.000399 0.000649 0.000189 0.000226 0.000279 0.000590 0.000229 0.000236 0.000337 0.000613
Cytoplasm 0.656457* 0.785220* 0.823452* 0.724004* 0.720590* 0.723265* 0.867898* 0.840211* 0.686565* 0.750922* 0.846596* 0.794198*
Cytoplasmic Foci 0.191196* 0.111788 0.044609 0.046234 0.150715 0.163938 0.022466 0.035727 0.172192 0.140658 0.033079 0.039888
Eisosomes 0.000023 0.000009 0.000009 0.000017 0.000012 0.000020 0.000007 0.000007 0.000018 0.000015 0.000008 0.000011
Endoplasmic Reticulum 0.028768 0.012756 0.004696 0.001180 0.019429 0.008817 0.001680 0.002128 0.024383 0.010575 0.003125 0.001753
Endosome 0.012413 0.007121 0.004600 0.007762 0.009807 0.008543 0.002489 0.003890 0.011189 0.007908 0.003500 0.005423
Golgi 0.015709 0.010479 0.003241 0.004686 0.011465 0.016335 0.002214 0.004521 0.013717 0.013721 0.002706 0.004586
Lipid Particles 0.004254 0.002715 0.012536 0.013471 0.003030 0.002783 0.007434 0.009599 0.003679 0.002753 0.009879 0.011133
Mitochondria 0.002582 0.000852 0.000114 0.001614 0.001185 0.001548 0.000213 0.000233 0.001926 0.001237 0.000165 0.000780
Mitotic Spindle 0.000549 0.000913 0.002887 0.033758 0.001317 0.002357 0.001181 0.002668 0.000910 0.001712 0.001999 0.014978
None 0.001825 0.000993 0.000826 0.002324 0.001035 0.001282 0.001422 0.000645 0.001454 0.001153 0.001136 0.001310
Nuclear Periphery 0.000085 0.000087 0.000119 0.000373 0.000144 0.000031 0.000110 0.000036 0.000113 0.000056 0.000114 0.000169
Nuclear Periphery Foci 0.001215 0.002151 0.009544 0.008779 0.001436 0.000521 0.007254 0.002986 0.001319 0.001249 0.008352 0.005280
Nucleolus 0.000181 0.000160 0.000163 0.000374 0.000224 0.000149 0.000294 0.000198 0.000201 0.000154 0.000231 0.000267
Nucleus 0.010415 0.007176 0.018658 0.040125 0.006576 0.005068 0.019382 0.008044 0.008613 0.006009 0.019035 0.020747
Peroxisomes 0.000592 0.000548 0.000312 0.002161 0.000452 0.000920 0.000312 0.000880 0.000526 0.000754 0.000312 0.001387
Vacuole 0.048056 0.036451 0.057681 0.060705 0.048161 0.033496 0.054226 0.063256 0.048105 0.034816 0.055882 0.062246
Vacuole Periphery 0.011202 0.005659 0.000897 0.002881 0.011856 0.007247 0.000906 0.002394 0.011509 0.006538 0.000902 0.002587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.28 -7.06 -4.34 -4.09 -3.37 -4.97 -4.96 -5.38 -1.27 -3.38 -4.05 -8.27 -5.31 -4.29 -3.83
Bud Neck 3.29 -7.10 -11.53 -12.30 -9.24 -3.05 -7.57 -9.14 -4.10 -1.82 -0.83 -10.17 -13.76 -12.72 -8.81
Bud Site -0.61 -0.85 -9.74 -9.22 -9.11 -8.16 2.66 -5.84 1.93 -8.47 -6.81 1.21 -11.08 -5.93 -11.80
Cell Periphery 0.38 -4.56 -4.53 -4.33 -2.12 -1.19 -3.79 -7.28 -5.74 -5.12 -0.40 -5.74 -8.22 -7.16 -4.88
Cytoplasm -15.17 -19.29 0.06 13.00 16.04 -0.32 -19.06 -12.32 -12.79 7.41 -10.73 -27.49 -10.41 -0.05 15.29
Cytoplasmic Foci 14.17 31.27 25.93 11.79 -3.19 -2.31 30.32 25.66 29.34 -7.98 7.79 43.61 37.69 30.17 -6.33
Eisosomes 10.42 10.11 3.39 -4.94 -4.48 -3.73 4.95 5.41 6.78 0.24 2.27 11.29 7.34 3.38 -3.34
Endoplasmic Reticulum 5.97 10.41 12.22 8.27 6.25 5.05 9.74 9.43 5.98 -0.30 8.06 14.21 15.22 9.92 3.41
Endosome 6.20 10.72 7.39 1.56 -1.58 1.99 14.21 10.97 9.33 -4.14 6.13 17.00 13.02 7.36 -3.10
Golgi 4.87 22.26 18.08 7.27 0.33 -5.20 14.57 10.14 14.09 -2.85 0.04 25.83 19.42 15.05 -2.20
Lipid Particles 5.59 -11.13 -10.29 -11.65 -2.15 0.75 -8.99 -9.72 -9.72 -1.02 4.18 -14.13 -13.78 -15.17 -1.70
Mitochondria 2.43 3.78 2.21 -0.09 -2.01 -0.87 2.91 2.89 5.34 -0.11 1.61 4.64 3.52 3.16 -2.04
Mitotic Spindle -1.49 -5.48 -10.10 -9.99 -9.10 -1.37 -0.59 -3.33 -2.13 -3.22 -1.91 -4.08 -10.55 -9.83 -9.36
None 1.41 1.83 -0.10 -3.65 -5.67 -0.48 -1.31 1.78 1.31 4.47 0.72 1.22 0.97 0.07 -0.60
Nuclear Periphery -0.10 -4.54 -5.86 -5.42 -4.66 3.87 0.39 2.70 -6.73 2.85 3.20 -1.34 -3.34 -5.98 -2.42
Nuclear Periphery Foci -4.28 -14.37 -10.39 -9.13 0.34 7.08 -10.11 -8.17 -12.45 6.51 0.45 -17.11 -12.87 -12.92 5.26
Nucleolus 0.99 1.49 -4.59 -4.76 -5.08 1.49 -1.28 0.20 -2.41 3.56 1.77 -1.04 -2.78 -5.19 -2.66
Nucleus 2.67 -7.75 -12.14 -14.45 -8.78 4.07 -21.85 -7.57 -10.61 13.04 3.98 -16.15 -12.40 -16.41 -2.36
Peroxisomes 0.37 7.41 -5.02 -4.74 -6.12 -4.60 3.58 -3.87 0.24 -5.23 -2.96 7.76 -6.17 -3.64 -7.99
Vacuole 4.19 -6.99 -14.83 -17.25 -10.12 5.54 -4.74 -10.45 -16.83 -6.84 7.05 -8.31 -17.93 -23.85 -11.68
Vacuole Periphery 6.07 13.47 11.24 6.43 -1.97 4.25 11.40 9.42 8.16 -2.63 7.07 17.49 14.35 10.25 -3.26