Standard name
Description Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0 0.05 0 0 0
Golgi 0 0.09 0 0 0 0 0 0 0 0 0 0.07 0.09 0.12 0.38 0.41 0.05 0.07 0 0.05 0.06 0.07
Mitochondria 0.85 0.88 0.96 0.95 0.84 0.86 0.86 0.95 0.91 0.93 0.94 0.87 0.86 0.81 0.19 0.06 0.71 0.81 0.75 0.75 0.73 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.05 0.05 0.06 0.09 0.14 0.1 0.14 0.13 0.1 0.14 0.1 0.06 0.06 0 0.12 0 0 0 0 0 0
Nucleolus 0.06 0 0.11 0.07 0.12 0.16 0.14 0.14 0.14 0.11 0.12 0.06 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0.07 0.05 0 0 0.06 0 0 0.11 0.05 0.06 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0 0 0.06 0 0 0 0 0 0 0.11 0.08 0.12 0.12 0.06 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 0 0 1 0 1 0 0 0 0 2 3 1 1 0 4 5 1 2 3 13
Bud 10 1 7 9 0 5 6 4 5 8 10 1 0 0 2 2 2 1 0 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2
Bud Site 1 0 3 1 0 2 1 4 4 0 2 0 0 0 0 0
Cell Periphery 3 6 3 4 2 3 1 0 4 1 1 3 8 8 1 1 0 0 0 0 0 0
Cytoplasm 16 1 4 6 3 6 6 3 2 3 2 0 4 8 4 2 11 0 0 0 0 0
Endoplasmic Reticulum 3 2 2 3 3 2 2 3 3 3 2 0 1 1 0 1 0 0 0 0 0 0
Endosome 7 1 1 0 13 5 5 1 0 0 1 8 14 13 0 0 27 2 10 3 3 9
Golgi 2 19 0 2 1 1 0 1 0 0 0 15 27 31 6 7 18 18 6 7 10 17
Mitochondria 318 193 202 244 206 274 323 327 258 300 298 186 256 216 3 1 268 205 159 111 127 153
Nucleus 4 0 0 1 0 1 3 0 1 2 0 0 0 0 0 0 2 0 0 1 0 0
Nuclear Periphery 17 11 10 15 23 43 36 49 38 32 43 22 17 16 0 2 2 1 1 2 2 2
Nucleolus 21 6 23 19 29 52 53 49 39 36 38 13 7 15 0 0 2 2 2 3 3 4
Peroxisomes 8 8 5 9 13 4 5 3 3 3 4 14 14 9 0 1 13 4 22 7 10 16
SpindlePole 2 1 1 1 2 0 4 4 4 1 0 2 4 2 0 0 3 0 1 0 1 1
Vac/Vac Membrane 59 37 8 5 15 10 15 7 5 7 8 24 24 31 2 1 19 8 1 4 7 8
Unique Cell Count 372 220 210 258 244 317 377 344 284 322 317 215 297 266 16 17 380 254 214 149 175 236
Labelled Cell Count 474 288 269 319 311 408 461 455 366 396 409 290 379 351 19 18 380 254 214 149 175 236


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 13.0 7.2 8.7 8.7 8.0 8.5 7.8 7.9 8.0 7.8 10.3 10.8 10.1 9.6 10.5 11.6 12.8 12.6
Std Deviation (1e-4) 2.0 2.6 1.9 2.9 2.2 2.2 2.8 2.7 2.7 2.5 2.6 2.1 2.3 2.3 2.3 2.8 2.9 3.2 3.1
Intensity Change (Log2) 0.27 0.28 0.15 0.24 0.13 0.14 0.16 0.13 0.52 0.59 0.49 0.43 0.55 0.69 0.83 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.1 0 0 0 0 -1.1 -0.6 -0.1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.8 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.0 0 0 0 0 0 0 0 2.8 2.8 0 0
Golgi 0 0 0 0 0 0 0 0 3.9 4.5 5.1 0 0
Mitochondria -0.8 -4.1 -3.7 -4.0 -0.6 -2.3 -1.5 -1.1 -3.5 -3.8 -5.0 -10.3 -11.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.5 1.9 3.3 2.1 3.5 3.2 2.2 3.3 2.1 0.5 0.6 0 0
Nucleolus -1.4 0.3 1.8 1.1 1.1 0.9 0.1 0.4 -1.8 -4.0 -2.1 0 0
Peroxisomes 0.7 1.6 0 0 0 0 0 0 2.1 1.4 0.6 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 1.1 -0.4 0.1 -1.2 -1.4 -1.1 -0.8 2.9 1.9 3.1 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available