Standard name
Human Ortholog
Description WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0 0 0.06 0 0 0 0 0 0.06 0.06 0 0 0.07 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.98 0.97 0.94 0.93 0.91 0.86 0.87 0.87 0.79 0.84 0.99 0.99 0.98 0.9 0.9 0.92 0.95 0.93 0.93 0.91 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.09 0.2 0.23 0.28 0.34 0.34 0.33 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 1 0 0 0 0 1 0 0 0 2 0 2 1 0 0 0 0 0 1 2
Bud 0 1 16 4 9 6 13 12 12 7 12 5 1 1 3 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 13 5 16 5 26 18 11 7 13 12 15 16 4 9 25 18 14 10 0 0 1 0 0 0
Cytoplasm 253 262 465 344 400 407 579 534 359 387 200 242 246 353 332 223 149 193 258 264 451 138 293 266
Endoplasmic Reticulum 7 7 1 1 5 0 1 0 0 3 0 0 1 2 2 10 7 6 1 1 8 6 11 16
Endosome 0 0 1 0 1 0 1 1 0 0 0 1 0 0 0 0 1 2 3 2 2 0 3 1
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 0 2 2 1 0 1 0
Mitochondria 0 4 8 34 37 86 145 170 141 152 83 89 0 1 6 3 3 4 2 5 7 1 0 3
Nucleus 1 0 1 2 0 3 0 2 0 3 1 3 1 1 0 3 4 4 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 0 7 2 0 0 0 2 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 7 1 7 2 4 11 0 2 1 1 1 1 1 22 14 11 1 3 8 1 1 4
Unique Cell Count 261 272 475 353 424 438 639 618 415 445 254 289 248 358 338 249 166 210 273 283 485 152 320 305
Labelled Cell Count 280 279 515 392 486 523 754 739 526 569 319 360 254 370 369 288 196 231 273 283 485 152 320 305


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 9.2 6.4 5.9 6.3 5.3 4.3 4.2 3.8 3.7 6.1 4.6 8.7 8.3 7.5 11.9 13.1 12.3 9.7 10.2 10.3
Std Deviation (1e-4) 1.7 1.4 0.8 1.0 3.2 3.3 1.0 0.9 0.8 1.0 6.9 4.7 1.2 1.1 1.0 2.0 1.7 1.9 1.6 1.6 1.6
Intensity Change (Log2) -0.11 -0.03 -0.25 -0.55 -0.6 -0.76 -0.76 -0.06 -0.46 0.45 0.39 0.23 0.9 1.05 0.95 0.6 0.68 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -2.1 -1.1 -2.0 0 0 0 0 0.9 0 -2.5 -3.1 -2.3 -2.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 2.0 0.6 0 0 0 0 1.6 0 -1.4 -0.7 2.6 2.3 2.7 0.9
Cytoplasm -0.4 -2.8 -3.6 -5.0 -6.7 -6.5 -6.3 -8.7 -7.2 1.3 0.8 0.3 -4.9 -4.5 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.2 4.8 8.9 0 0 0 0 12.1 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.2 0 0 0.4 0 0 0 0 0 0 0 4.8 4.3 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7685 8.7752 7.6719 7.5159 6.7339 7.815 8.0226 10.1353 10.0272 9.5528 9.0648 9.7668 9.1021 10.907 9.8683 9.3582 9.9464 9.9769
Actin 0.0317 0.0002 0.0131 0.0018 0.0017 0.005 0.0145 0.0019 0.0072 0.001 0.0257 0.0005 0.0009 0.0002 0.0021 0.027 0.0001 0.0011
Bud 0.0005 0.0002 0.0005 0.0003 0.0005 0.0003 0.0011 0.0006 0.0011 0.0008 0.0029 0.0001 0.0169 0.0002 0.0108 0.0081 0.0001 0.008
Bud Neck 0.0026 0.0002 0.0006 0.0006 0.0009 0.0016 0.0019 0.0005 0.0015 0.0005 0.0088 0.0006 0.0002 0.0002 0.0011 0.0007 0.0003 0.0012
Bud Periphery 0.0006 0.0001 0.0006 0.0001 0.0003 0.0003 0.0011 0.0003 0.0008 0.0011 0.0066 0.0001 0.0035 0.0001 0.0035 0.0041 0 0.0023
Bud Site 0.0028 0.0004 0.001 0.0003 0.0005 0.0002 0.0041 0.0088 0.0039 0.0016 0.0054 0.0001 0.0039 0.0005 0.0093 0.0025 0.0001 0.0003
Cell Periphery 0.0009 0.0003 0.0003 0.0002 0.0002 0.0004 0.0003 0.0002 0.0002 0.0004 0.0005 0 0.001 0.0001 0.0004 0.0002 0 0.0001
Cytoplasm 0.8082 0.9759 0.9094 0.9046 0.9074 0.9335 0.8172 0.9546 0.9037 0.8537 0.8271 0.9398 0.8979 0.9432 0.8958 0.8927 0.9452 0.9397
Cytoplasmic Foci 0.0114 0.0029 0.0084 0.0088 0.0089 0.0064 0.0247 0.0085 0.0139 0.0172 0.0178 0.01 0.0178 0.0046 0.016 0.0173 0.0145 0.0121
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0294 0.0072 0.0149 0.013 0.0032 0.0204 0.018 0.0043 0.0068 0.0127 0.002 0.0071 0.0106 0.0029 0.0062 0.0025 0.0076 0.0058
Endosome 0.0337 0.0048 0.021 0.0194 0.0324 0.0141 0.0475 0.0079 0.0261 0.0334 0.0374 0.0205 0.0172 0.0096 0.03 0.0299 0.0238 0.0207
Golgi 0.0078 0.0004 0.0038 0.0016 0.0133 0.0028 0.0088 0.0013 0.0069 0.0132 0.0276 0.0072 0.0012 0.0007 0.003 0.0015 0.0007 0.0017
Lipid Particles 0.0152 0 0.0017 0.0016 0.0032 0.0024 0.002 0.0001 0.0007 0.0103 0.003 0.0003 0.0006 0 0.0007 0.0002 0.0001 0.0001
Mitochondria 0.0038 0.0002 0.0097 0.0004 0.0047 0.0046 0.0064 0.0032 0.0039 0.0139 0.0215 0.0082 0.0015 0.0004 0.0009 0.0005 0.0001 0.0007
None 0.0085 0.0018 0.0028 0.001 0.0022 0.0012 0.0119 0.0033 0.0025 0.0017 0.0011 0.0017 0.0178 0.0344 0.0107 0.0016 0.0014 0.0024
Nuclear Periphery 0.0136 0.001 0.0024 0.0014 0.0077 0.0012 0.0116 0.0005 0.0032 0.0015 0.0005 0.0004 0.0011 0.0004 0.0008 0.0008 0.0005 0.0004
Nucleolus 0.0006 0 0.0002 0 0.0003 0 0.0007 0.0001 0.0002 0.0001 0.0002 0 0.0004 0 0.0012 0 0 0
Nucleus 0.0039 0.0008 0.0016 0.001 0.0024 0.001 0.0148 0.0008 0.0056 0.0014 0.0011 0.0007 0.0015 0.0005 0.0012 0.0009 0.0006 0.0007
Peroxisomes 0.0049 0 0.0008 0.0319 0.0008 0.0009 0.0023 0.0008 0.0009 0.0104 0.0052 0.0004 0.0002 0 0.0004 0.0007 0 0.0001
Punctate Nuclear 0.0088 0 0.0009 0.0002 0.0015 0.0006 0.0022 0.0001 0.0006 0.0003 0.0002 0.0001 0.0004 0 0.0003 0.0006 0.0001 0.0002
Vacuole 0.009 0.0031 0.0052 0.0102 0.006 0.0023 0.0069 0.0019 0.0089 0.0222 0.0042 0.0015 0.0048 0.0014 0.0048 0.0069 0.004 0.0016
Vacuole Periphery 0.0019 0.0003 0.0012 0.0017 0.0018 0.0007 0.0017 0.0003 0.0013 0.0025 0.0013 0.0005 0.0008 0.0004 0.0008 0.0014 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.2874 27.8857 28.3919 31.0233 30.3335 16.3135 24.261 22.2384 22.0615 32.5324
Translational Efficiency 0.8657 0.8912 0.8726 0.6531 0.7252 1.1045 0.8658 0.631 0.6401 0.6681

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
292 1490 2597 260 1469 1856 987 97 1761 3346 3584 357

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 841.34 1095.22 1244.17 1133.81 945.97 1045.93 1091.19 1452.34 928.62 1067.88 1202.04 1220.36
Standard Deviation 106.15 157.54 160.28 130.76 118.01 151.15 191.55 197.53 122.48 155.96 182.73 207.68
Intensity Change Log 2 0.380460 0.564423 0.430418 0.144920 0.206036 0.618513 0.260596 0.385855 0.533016

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000199 0.000237 0.000060 0.000242 0.000271 0.000385 0.000132 0.000087 0.000259 0.000319 0.000080 0.000200
Bud Neck 0.004590 0.000802 0.000392 0.001803 0.001758 0.001581 0.002301 0.004144 0.002228 0.001234 0.000918 0.002439
Bud Site 0.030116 0.018314 0.004394 0.017089 0.022441 0.020379 0.006463 0.033550 0.023714 0.019459 0.004964 0.021562
Cell Periphery 0.000288 0.000111 0.000032 0.000089 0.000261 0.000108 0.000459 0.002257 0.000266 0.000109 0.000150 0.000678
Cytoplasm 0.692165* 0.800131* 0.941380* 0.667506* 0.720860* 0.738842* 0.579151* 0.868828* 0.716102* 0.766134* 0.841626* 0.722207*
Cytoplasmic Foci 0.188674* 0.120928 0.009108 0.089963 0.175950 0.151147 0.056793 0.004025 0.178060 0.137690 0.022240 0.066613
Eisosomes 0.000069 0.000009 0.000001 0.000014 0.000034 0.000014 0.000118 0.000009 0.000039 0.000012 0.000033 0.000013
Endoplasmic Reticulum 0.000971 0.001437 0.000115 0.000438 0.004022 0.001225 0.000971 0.000014 0.003516 0.001320 0.000351 0.000323
Endosome 0.014258 0.008237 0.000255 0.007277 0.011353 0.011343 0.006113 0.000539 0.011835 0.009960 0.001869 0.005446
Golgi 0.007926 0.008510 0.000208 0.000443 0.012579 0.009520 0.000255 0.000678 0.011807 0.009070 0.000221 0.000507
Lipid Particles 0.002229 0.000696 0.000181 0.000968 0.001651 0.000748 0.002461 0.000076 0.001747 0.000724 0.000809 0.000726
Mitochondria 0.003326 0.001319 0.000037 0.000020 0.003146 0.001343 0.000253 0.000326 0.003176 0.001332 0.000097 0.000103
Mitotic Spindle 0.000170 0.003443 0.000453 0.000475 0.000929 0.004962 0.002934 0.000335 0.000803 0.004286 0.001137 0.000437
None 0.001528 0.000700 0.002392 0.001164 0.001506 0.000745 0.006727 0.000784 0.001509 0.000725 0.003586 0.001061
Nuclear Periphery 0.000032 0.000040 0.000025 0.000091 0.000029 0.000041 0.000346 0.000005 0.000029 0.000040 0.000113 0.000068
Nuclear Periphery Foci 0.000696 0.000506 0.000588 0.003659 0.000426 0.000386 0.003278 0.000039 0.000471 0.000440 0.001329 0.002675
Nucleolus 0.000214 0.000094 0.000075 0.000199 0.000089 0.000107 0.000451 0.000273 0.000109 0.000101 0.000179 0.000219
Nucleus 0.006879 0.003074 0.026108 0.020234 0.005187 0.004286 0.157281* 0.004643 0.005468 0.003746 0.062232 0.015998
Peroxisomes 0.001203 0.000780 0.000080 0.000794 0.000686 0.000951 0.000375 0.000556 0.000772 0.000874 0.000161 0.000729
Vacuole 0.042065 0.025935 0.014011 0.187093 0.029672 0.047465 0.172805 0.076378 0.031727 0.037878 0.057741 0.157011
Vacuole Periphery 0.002400 0.004699 0.000103 0.000438 0.007151 0.004422 0.000331 0.002452 0.006364 0.004545 0.000166 0.000985

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 4.57 -0.43 0.53 -5.01 -0.78 4.76 7.85 2.05 3.88 -0.74 8.74 2.75 1.63 -3.98
Bud Neck 7.30 7.99 5.35 -5.52 -7.87 0.81 -1.25 -2.37 -2.54 -2.05 5.66 8.34 -0.41 -3.64 -4.88
Bud Site 3.96 9.21 4.37 1.39 -5.33 1.25 11.03 -1.17 -1.40 -2.86 3.11 15.78 1.19 -0.20 -5.17
Cell Periphery 5.92 8.59 6.83 3.34 -5.60 9.08 -0.78 -1.40 -1.51 -1.33 10.84 4.37 -1.03 -1.44 -1.41
Cytoplasm -7.60 -17.65 1.74 8.23 16.03 -2.11 11.13 -8.05 -7.14 -13.85 -7.15 -17.39 0.21 3.66 8.35
Cytoplasmic Foci 6.66 18.99 9.68 5.51 -10.26 4.23 23.84 36.58 35.72 19.46 8.22 37.44 18.45 13.66 -6.72
Eisosomes 8.01 9.02 7.22 -3.34 -9.13 6.62 -6.47 8.82 2.13 9.42 11.05 4.16 10.71 -0.56 4.94
Endoplasmic Reticulum -1.18 3.69 2.60 3.74 -3.78 4.50 5.55 7.04 4.85 8.02 4.30 6.72 6.79 5.50 0.50
Endosome 2.84 6.87 3.79 2.15 -6.09 0.02 7.45 15.95 15.56 11.54 2.36 15.37 7.98 6.76 -3.84
Golgi -0.61 9.70 9.50 11.47 -3.87 3.32 26.28 24.23 10.80 -2.80 3.98 27.95 27.22 15.71 -4.48
Lipid Particles 3.19 4.14 2.80 -1.40 -5.98 6.64 -1.13 12.19 15.16 5.90 7.28 6.13 6.98 0.71 0.67
Mitochondria 0.68 1.12 1.13 2.56 4.88 1.64 2.92 2.80 2.25 -1.01 1.79 3.19 3.17 3.66 -0.62
Mitotic Spindle -2.96 -4.09 -1.46 2.26 -0.40 -3.44 -1.84 1.37 3.96 2.39 -4.15 -1.13 0.37 4.26 1.40
None 2.05 -1.62 1.08 -0.81 3.52 1.75 -2.41 1.71 -0.12 3.02 2.20 -2.80 1.40 -0.75 4.43
Nuclear Periphery -0.27 -0.64 -5.84 -4.26 -8.37 -0.74 -13.06 6.09 2.17 14.18 -0.91 -12.02 -7.22 -3.32 4.24
Nuclear Periphery Foci 0.92 -0.09 -5.96 -7.18 -6.66 0.37 -10.16 6.77 5.72 11.43 0.42 -9.59 -6.65 -6.79 -3.38
Nucleolus 1.18 1.40 0.49 -2.06 -4.25 -1.17 -5.03 -4.84 -3.90 1.41 0.29 -1.75 -3.26 -3.76 -1.93
Nucleus 2.90 -15.23 -5.38 -7.83 3.67 1.09 -17.58 0.40 -0.40 17.59 2.53 -23.92 -6.11 -7.42 14.25
Peroxisomes 2.65 8.70 3.60 1.16 -6.02 -2.99 6.03 0.85 2.37 -1.32 -1.58 16.27 1.81 2.68 -5.79
Vacuole 3.76 4.17 -11.53 -13.11 -13.23 -7.03 -22.12 -3.77 -2.32 7.97 -3.17 -13.70 -12.64 -12.10 -9.07
Vacuole Periphery -3.31 5.30 4.70 8.02 -5.21 3.33 9.79 4.82 2.45 -3.06 2.68 10.54 8.78 9.24 -3.84