Standard name
Human Ortholog
Description Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.96 0.97 0.97 0.95 0.79 0.7 0.7 0.55 0.58 0.48 0.47 1.0 1.0 1.0 0.97 0.96 0.97 0.88 0.91 0.8 0.91 0.87 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.05 0.08 0.42 0.47 0.42 0.66 0.63 0.67 0.76 0 0 0 0.05 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 7 0 1 3
Bud 0 0 0 0 0 8 8 16 7 15 7 4 1 1 0 0 0 1 1 0 5 1 6 5
Bud Neck 0 0 0 0 0 1 1 2 1 0 1 0 0 1 0 0 1 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 6 2 4 0 1 4 2 3 0 1 0 3 2 6 3 5 0 0 0 0 0 2
Cytoplasm 149 334 317 234 303 272 338 381 202 237 95 109 340 388 488 454 257 194 147 318 263 136 298 255
Endoplasmic Reticulum 0 3 5 0 2 2 0 2 4 0 4 1 0 1 1 30 19 12 0 1 4 0 2 0
Endosome 0 0 1 4 5 0 2 5 1 0 0 0 0 0 0 5 4 0 4 3 7 1 4 1
Golgi 0 2 1 1 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 4 1 1 3 0
Mitochondria 25 6 7 13 24 144 224 231 242 259 132 175 1 0 0 24 1 2 3 6 22 1 6 10
Nucleus 0 0 1 0 0 0 0 1 0 0 0 2 0 2 1 1 2 1 0 2 0 0 0 0
Nuclear Periphery 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 1 10 15 3 5 5 0 0 0 0 1 3 0 1 2 1 2 6 4
Vac/Vac Membrane 1 3 2 0 5 9 9 11 4 4 3 6 0 0 0 12 9 5 3 3 3 1 6 12
Unique Cell Count 161 347 328 241 318 344 480 548 364 409 198 231 340 389 490 470 267 200 167 348 329 150 344 306
Labelled Cell Count 175 351 342 255 343 437 594 670 467 524 250 298 342 396 493 533 300 221 167 348 329 150 344 306


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.1 4.6 4.5 4.1 3.3 2.9 3.2 3.0 2.7 2.9 2.6 4.7 4.4 4.2 5.5 6.9 6.2 4.4 4.7 5.4
Std Deviation (1e-4) 0.7 0.9 1.3 1.9 1.7 0.9 1.2 1.3 0.9 0.7 1.0 0.6 1.3 1.4 1.2 1.4 1.7 1.4 1.4 1.8 1.6
Intensity Change (Log2) – – – -0.03 -0.16 -0.48 -0.66 -0.52 -0.62 -0.79 -0.69 -0.82 0.02 -0.05 -0.13 0.25 0.58 0.43 -0.08 0.04 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.9 -6.9 -9.3 -9.7 -12.5 -12.1 -13.1 -13.5 3.4 3.2 3.3 0 -0.3 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.4 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.0 0 0 0 0 0 0 0 0 0 2.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1523 0.3577 0.8053 0.3162 0.3884 0.5531 1.8713 2.1242 2.2996 2.4892 1.8236 2.3233 2.6107 2.1924 3.4185 2.8559 4.3242 2.7886
Actin 0.0054 0.0015 0.0003 0.0001 0.0006 0.0005 0.0801 0.0033 0.0194 0.0017 0.0001 0.0047 0.006 0.0004 0.0491 0.1003 0.0004 0.0012
Bud 0.0001 0.0021 0.0001 0 0 0 0.0018 0.0069 0.0007 0.0001 0.0001 0.0003 0.0029 0.0069 0.0005 0.0009 0.0003 0.0001
Bud Neck 0.0001 0.0003 0.0004 0 0.0001 0.0002 0.0058 0.0006 0.0003 0.0002 0 0.0001 0.0095 0.0002 0.0298 0.0009 0.0052 0.0003
Bud Periphery 0.0002 0.0083 0.0002 0 0.0001 0.0001 0.0035 0.0122 0.0013 0.0001 0 0.0011 0.0044 0.0028 0.001 0.0021 0.0003 0.0002
Bud Site 0.0003 0.0135 0.0003 0 0.0005 0 0.0163 0.0289 0.0035 0.0005 0 0 0.0151 0.0241 0.0188 0.0008 0.0221 0.0001
Cell Periphery 0.0002 0.0003 0.0003 0 0.0001 0 0.0009 0.0003 0.0003 0 0 0.0001 0.0005 0.0002 0.0018 0.0002 0.0002 0.0001
Cytoplasm 0.0542 0.089 0.0986 0.1693 0.0983 0.1479 0.1767 0.2113 0.2197 0.1402 0.1591 0.2092 0.0983 0.2041 0.1198 0.1042 0.1892 0.1938
Cytoplasmic Foci 0.0123 0.0132 0.0044 0.0049 0.017 0.0066 0.0224 0.0106 0.0295 0.0805 0.0039 0.0099 0.0235 0.0035 0.0082 0.0365 0.0687 0.0093
Eisosomes 0.0006 0.0004 0.0003 0 0.0001 0.0001 0.0006 0.0001 0.0005 0.0001 0 0.0001 0.0003 0.0001 0.0023 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.0055 0.0048 0.0022 0.0007 0.001 0.0013 0.0043 0.0015 0.0015 0.0006 0.0006 0.0018 0.0103 0.0058 0.0019 0.0005 0.0004 0.0011
Endosome 0.0529 0.0079 0.0105 0.0011 0.0059 0.0037 0.022 0.0011 0.0148 0.0288 0.0014 0.0071 0.0408 0.0022 0.0047 0.0131 0.007 0.0035
Golgi 0.0029 0.0008 0.0026 0.0001 0.0024 0.0002 0.0091 0.0008 0.0122 0.042 0.0001 0.0013 0.0043 0.0001 0.0054 0.0095 0.0034 0.0029
Lipid Particles 0.02 0.0011 0.0004 0.0004 0.0246 0.0004 0.006 0.0015 0.0185 0.0205 0.0001 0.0039 0.0044 0.0003 0.0039 0.0206 0.0444 0.0024
Mitochondria 0.0032 0.0012 0.0073 0.0002 0.0025 0.0003 0.002 0.0021 0.0032 0.0043 0.0001 0.0005 0.0024 0.0008 0.016 0.0262 0.0005 0.0084
None 0.8096 0.8476 0.8673 0.8213 0.8375 0.8368 0.63 0.7064 0.6498 0.598 0.833 0.7558 0.7234 0.7309 0.7299 0.6244 0.6096 0.7731
Nuclear Periphery 0.0135 0.0016 0.0005 0.0004 0.0002 0.0004 0.0019 0.0003 0.0006 0.0004 0.0002 0.0009 0.0207 0.0055 0.0005 0.0003 0.0002 0.0004
Nucleolus 0.0005 0.0008 0.0002 0.0001 0.0002 0.0001 0.0004 0.0003 0.0013 0.0001 0 0.0001 0.0021 0.0004 0.0004 0.0004 0.0042 0.0001
Nucleus 0.0019 0.0009 0.0005 0.0002 0.0002 0.0004 0.0009 0.0006 0.0007 0.0002 0.0002 0.0004 0.0082 0.0072 0.0004 0.0004 0.0006 0.0004
Peroxisomes 0.0025 0.0005 0.0002 0.0001 0.0076 0.0002 0.0103 0.0099 0.0141 0.0794 0 0.0006 0.0027 0.0002 0.0043 0.0542 0.0278 0.0008
Punctate Nuclear 0.0034 0.0021 0.0004 0.0002 0.0006 0.0004 0.0027 0.0005 0.0062 0.0007 0.0002 0.0013 0.0098 0.0024 0.0006 0.0017 0.0143 0.001
Vacuole 0.0067 0.0015 0.002 0.0007 0.0005 0.0006 0.002 0.0007 0.0014 0.0012 0.0006 0.0005 0.0084 0.0015 0.0005 0.0016 0.001 0.0006
Vacuole Periphery 0.0039 0.0005 0.0012 0.0001 0.0001 0.0001 0.0005 0.0001 0.0004 0.0003 0.0001 0.0002 0.002 0.0004 0.0001 0.0006 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.1455 35.8682 33.2697 50.0462 39.0167 36.0914 43.5196 47.3228 45.4647 54.3606
Translational Efficiency 0.6516 0.6626 0.7304 0.4261 0.5725 0.9282 0.6309 0.5981 0.6972 0.78

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
415 1351 276 1476 2199 1424 445 417 2614 2775 721 1893

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 692.48 687.71 853.84 839.74 672.60 679.58 855.33 1156.27 675.76 683.54 854.76 909.47
Standard Deviation 71.07 111.47 80.21 130.48 72.20 87.25 103.10 174.06 72.39 99.86 95.00 192.76
Intensity Change Log 2 — -0.009972 0.302193 0.278170 — 0.014895 0.346732 0.781658 — 0.002334 0.324310 0.548133

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000307 0.000247 0.000890 0.000298 0.000291 0.000207 0.000149 0.000265 0.000299 0.000222 0.000727
Bud Neck 0.012076 0.021158 0.002080 0.016442 0.014386 0.024729 0.002092 0.025524 0.014019 0.022990 0.002087 0.018443
Bud Site 0.002706 0.014141 0.005713 0.051544 0.003256 0.009159 0.006680 0.037407 0.003169 0.011584 0.006310 0.048430
Cell Periphery 0.000206 0.000119 0.000151 0.000356 0.000087 0.000109 0.000132 0.000074 0.000106 0.000114 0.000139 0.000294
Cytoplasm 0.531358* 0.509298* 0.294443* 0.474826* 0.526064* 0.480189* 0.327487* 0.628375* 0.526904* 0.494360* 0.314838* 0.508651*
Cytoplasmic Foci 0.235695* 0.180501* 0.018942 0.011011 0.195644* 0.227750* 0.015644 0.005210 0.202003* 0.204747* 0.016907 0.009733
Eisosomes 0.000205 0.000084 0.000074 0.000031 0.000120 0.000093 0.000069 0.000010 0.000133 0.000089 0.000071 0.000027
Endoplasmic Reticulum 0.000572 0.000812 0.004084 0.001783 0.000747 0.000487 0.002443 0.000338 0.000720 0.000645 0.003071 0.001465
Endosome 0.014805 0.021959 0.004263 0.006141 0.009550 0.021076 0.002606 0.002683 0.010384 0.021506 0.003240 0.005379
Golgi 0.001116 0.002632 0.000076 0.006111 0.001717 0.003144 0.000206 0.001339 0.001622 0.002895 0.000156 0.005060
Lipid Particles 0.008527 0.005066 0.002979 0.000742 0.006071 0.004520 0.001869 0.000175 0.006461 0.004786 0.002294 0.000617
Mitochondria 0.000842 0.006334 0.000334 0.006146 0.002129 0.005851 0.000397 0.009712 0.001925 0.006086 0.000373 0.006931
Mitotic Spindle 0.000031 0.004021 0.000200 0.090149 0.000473 0.007477 0.009923 0.032610 0.000403 0.005794 0.006201 0.077474
None 0.004378 0.005660 0.002663 0.007752 0.001672 0.002637 0.007630 0.008158 0.002101 0.004109 0.005729 0.007841
Nuclear Periphery 0.000327 0.000509 0.001666 0.001530 0.000536 0.000383 0.001563 0.000662 0.000503 0.000445 0.001603 0.001339
Nuclear Periphery Foci 0.000414 0.000604 0.002266 0.000985 0.000253 0.000409 0.001128 0.000043 0.000279 0.000504 0.001564 0.000778
Nucleolus 0.000706 0.002267 0.000600 0.001087 0.000896 0.001936 0.000710 0.000359 0.000866 0.002097 0.000668 0.000926
Nucleus 0.109912 0.104381 0.561325* 0.242910* 0.170431* 0.095589 0.519247* 0.202417* 0.160823* 0.099870 0.535354* 0.233990*
Peroxisomes 0.002670 0.007127 0.000403 0.001155 0.003356 0.009825 0.000281 0.000429 0.003247 0.008512 0.000328 0.000995
Vacuole 0.072650 0.110754 0.097297 0.076696 0.061609 0.102753 0.099117 0.039720 0.063362 0.106648 0.098420 0.068551
Vacuole Periphery 0.000711 0.002267 0.000192 0.001713 0.000706 0.001592 0.000570 0.004605 0.000707 0.001921 0.000425 0.002350

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.39 -4.01 -3.55 -1.98 -1.81 0.06 0.11 0.57 1.42 2.39 -0.19 -0.19 -1.13 -1.77 -1.87
Bud Neck -3.03 4.20 0.26 4.80 -8.15 -4.66 12.04 -0.18 2.76 -4.55 -5.49 12.73 1.46 6.50 -9.69
Bud Site -6.98 -0.70 -8.62 -3.39 -5.36 -6.58 -0.62 -5.19 -3.17 -4.45 -8.91 -0.84 -9.65 -5.14 -7.64
Cell Periphery 2.62 1.05 0.56 -1.12 -0.09 -0.80 -3.12 5.22 2.04 6.50 -0.40 -3.14 -1.04 -0.83 -0.19
Cytoplasm 1.83 14.12 13.09 14.35 -5.19 5.90 21.84 3.45 -0.34 -12.49 5.24 26.29 18.09 12.63 -11.17
Cytoplasmic Foci 5.49 23.65 25.46 33.03 5.51 -4.78 43.29 45.69 39.59 5.13 -0.48 48.21 51.40 50.86 6.26
Eisosomes 2.28 2.05 2.98 9.37 6.43 3.67 4.44 14.99 22.71 13.77 4.31 4.15 9.07 17.73 11.26
Endoplasmic Reticulum -2.12 -10.44 -14.36 -14.86 4.83 2.60 -11.52 1.14 -1.99 12.26 0.82 -15.20 -13.06 -16.02 7.99
Endosome -3.23 6.05 7.93 13.32 4.34 -8.38 11.85 13.14 15.79 4.03 -10.08 11.91 13.92 19.84 3.92
Golgi -3.00 4.71 -0.41 1.26 -1.83 -2.58 7.29 4.63 5.17 -1.29 -3.24 8.13 0.66 2.40 -1.91
Lipid Particles 1.91 2.73 4.00 9.20 6.51 3.08 9.13 13.89 14.62 8.29 3.34 8.08 12.14 15.11 8.66
Mitochondria -5.21 1.99 -2.31 3.12 -3.24 -3.38 6.42 -1.66 1.33 -3.44 -5.35 6.80 -1.38 3.51 -4.24
Mitotic Spindle -3.99 -2.88 -10.99 -9.61 -10.91 -5.48 -2.60 -5.01 -2.06 -2.79 -6.44 -2.59 -11.88 -8.71 -9.07
None -0.47 1.08 -1.37 -1.38 -10.30 -2.74 -5.48 -12.34 -9.53 -5.87 -3.10 -4.10 -12.67 -5.78 -6.57
Nuclear Periphery -2.95 -14.55 -24.84 -23.28 -9.59 1.31 -13.02 -8.39 -10.10 -0.19 0.50 -15.13 -20.20 -26.38 -8.09
Nuclear Periphery Foci -2.20 -7.66 -7.22 -4.18 5.71 -1.73 -6.19 4.94 2.05 6.86 -2.39 -9.94 -9.75 -0.20 6.47
Nucleolus -3.86 1.25 -0.90 3.22 -1.49 -3.63 2.64 -0.47 3.29 -1.63 -4.68 2.85 -0.17 4.51 -2.16
Nucleus 0.62 -22.74 -26.49 -30.94 9.62 15.44 -24.43 -14.06 -19.88 8.56 15.08 -32.06 -27.72 -38.13 13.84
Peroxisomes -3.85 2.75 2.77 9.53 0.29 -7.67 10.10 10.13 11.76 0.62 -8.69 10.07 9.68 14.93 -0.68
Vacuole -7.28 -6.19 -15.53 -9.98 -2.93 -10.05 -10.64 -3.85 1.94 7.25 -13.95 -12.59 -19.10 -9.91 0.51
Vacuole Periphery -3.86 6.64 1.22 4.18 -2.50 -4.54 3.25 -0.53 0.40 -1.02 -5.45 5.09 0.15 3.97 -1.86
Standard name
Human Ortholog
Description Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family
Localization
Cell Percentages cytoplasm (34%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ppq1

Ppq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available