Standard name
Description Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.96 0.97 0.97 0.95 0.79 0.7 0.7 0.55 0.58 0.48 0.47 1.0 1.0 1.0 0.97 0.96 0.97 0.88 0.91 0.8 0.91 0.87 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0.05 0.08 0.42 0.47 0.42 0.66 0.63 0.67 0.76 0 0 0 0.05 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 7 0 1 3
Bud 0 0 0 0 0 8 8 16 7 15 7 4 1 1 0 0 0 1 1 0 5 1 6 5
Bud Neck 0 0 0 0 0 1 1 2 1 0 1 0 0 1 0 0 1 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0
Cell Periphery 0 0 6 2 4 0 1 4 2 3 0 1 0 3 2 6 3 5 0 0 0 0 0 2
Cytoplasm 149 334 317 234 303 272 338 381 202 237 95 109 340 388 488 454 257 194 147 318 263 136 298 255
Endoplasmic Reticulum 0 3 5 0 2 2 0 2 4 0 4 1 0 1 1 30 19 12 0 1 4 0 2 0
Endosome 0 0 1 4 5 0 2 5 1 0 0 0 0 0 0 5 4 0 4 3 7 1 4 1
Golgi 0 2 1 1 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 4 1 1 3 0
Mitochondria 25 6 7 13 24 144 224 231 242 259 132 175 1 0 0 24 1 2 3 6 22 1 6 10
Nucleus 0 0 1 0 0 0 0 1 0 0 0 2 0 2 1 1 2 1 0 2 0 0 0 0
Nuclear Periphery 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 1 10 15 3 5 5 0 0 0 0 1 3 0 1 2 1 2 6 4
Vac/Vac Membrane 1 3 2 0 5 9 9 11 4 4 3 6 0 0 0 12 9 5 3 3 3 1 6 12
Unique Cell Count 161 347 328 241 318 344 480 548 364 409 198 231 340 389 490 470 267 200 167 348 329 150 344 306
Labelled Cell Count 175 351 342 255 343 437 594 670 467 524 250 298 342 396 493 533 300 221 167 348 329 150 344 306


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.1 4.6 4.5 4.1 3.3 2.9 3.2 3.0 2.7 2.9 2.6 4.7 4.4 4.2 5.5 6.9 6.2 4.4 4.7 5.4
Std Deviation (1e-4) 0.7 0.9 1.3 1.9 1.7 0.9 1.2 1.3 0.9 0.7 1.0 0.6 1.3 1.4 1.2 1.4 1.7 1.4 1.4 1.8 1.6
Intensity Change (Log2) -0.03 -0.16 -0.48 -0.66 -0.52 -0.62 -0.79 -0.69 -0.82 0.02 -0.05 -0.13 0.25 0.58 0.43 -0.08 0.04 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.9 -6.9 -9.3 -9.7 -12.5 -12.1 -13.1 -13.5 3.4 3.2 3.3 0 -0.3 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.4 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.0 0 0 0 0 0 0 0 0 0 2.1 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available