Standard name
Description UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.96 0.98 0.96 0.93 0.93 0.8 0.81 0.71 0.6 0.57 0.94 0.95 0.99 0.98 0.97 0.44 0.79 0.72 0.67 0.7 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.25 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.05 0 0.05 0 0.16 0.37 0.29 0.42 0.59 0.59 0 0 0 0 0 0.07 0.06 0.11 0.09 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 7 3 1 4
Bud 0 2 3 0 1 0 2 1 1 5 6 0 0 0 0 0 0 4 1 4 3 2
Bud Neck 0 0 2 0 1 0 0 1 1 0 2 0 1 0 7 0 0 2 2 2 2 3
Bud Site 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cell Periphery 0 0 0 3 1 1 2 3 4 1 2 1 1 1 4 8 2 1 3 3 1 2
Cytoplasm 58 231 223 126 117 174 160 181 142 159 167 84 84 120 451 281 56 186 172 82 106 106
Endoplasmic Reticulum 1 2 1 1 4 2 0 1 1 1 7 0 0 1 37 21 30 2 8 5 5 2
Endosome 2 0 0 1 1 1 0 0 3 3 2 1 0 0 1 2 5 5 3 1 1 1
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 1 0
Mitochondria 6 11 5 7 3 31 74 65 84 157 172 2 2 0 15 7 9 13 26 11 13 13
Nucleus 1 3 0 1 0 0 0 0 0 0 1 1 1 0 1 3 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 0 1 2 6 6 0 0 0 0 0 0 1 2 0 1 1
Vac/Vac Membrane 1 0 0 0 1 2 1 2 2 3 2 1 2 0 2 1 9 5 3 1 5 4
Unique Cell Count 67 241 227 131 126 188 199 224 199 264 293 89 88 121 460 290 126 238 239 123 152 149
Labelled Cell Count 71 252 235 139 130 211 241 257 242 338 367 90 91 123 518 323 126 238 239 123 152 149


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.0 3.7 3.6 3.6 3.2 2.9 3.3 2.9 3.0 2.8 5.3 5.3 5.3 4.3 5.1 5.7 5.0 5.9 6.4
Std Deviation (1e-4) 0.9 1.0 1.2 0.9 0.8 1.0 0.9 1.3 0.7 1.4 0.9 1.4 1.4 0.7 1.1 1.4 1.5 1.7 2.4 3.2
Intensity Change (Log2) -0.01 -0.03 -0.21 -0.35 -0.13 -0.34 -0.26 -0.38 0.53 0.53 0.55 0.22 0.47 0.64 0.46 0.69 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available