Standard name
Human Ortholog
Description Heme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.05 0 0.07 0 0 0.05 0.06 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.67 0.69 0.79 0.74 0.84 0.71 0.62 0.55 0.57 0.59 0.59 0.62 0.81 0.91 0.93 0.73 0.71 0.73 0.26 0.51 0.4 0.38 0.53 0.51
Endoplasmic Reticulum 0.33 0.28 0.17 0 0.14 0.2 0.2 0.15 0.19 0.2 0.15 0.21 0.17 0.08 0.08 0 0 0 0.56 0.28 0.51 0.38 0.21 0.21
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0 0.05 0 0.1 0.05 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0.25 0 0.06 0.15 0.27 0.18 0.15 0.12 0.11 0.05 0 0 0.23 0.25 0.21 0.06 0.09 0 0.12 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 5 0 2 7 2 2 0 0 0 0 1 0 0 2 1 3 5 10
Bud 0 2 2 3 5 9 7 11 7 7 7 9 4 2 0 1 1 0 0 2 0 4 10 25
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 3 6 13
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 0 2 3 14 12 15 17 25 6 24 11 22 40 17 22 12 0 1 0 2 3 6
Cytoplasm 18 109 55 143 335 299 354 399 212 319 119 211 351 583 789 193 174 123 22 102 37 93 215 297
Endoplasmic Reticulum 9 44 12 5 54 85 117 112 71 110 31 70 75 51 68 5 1 2 48 55 48 94 85 123
Endosome 0 0 0 1 1 0 0 1 0 0 1 0 0 2 1 4 0 0 0 1 0 2 1 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 2 0
Mitochondria 0 0 1 10 3 14 30 29 36 26 25 31 0 0 0 1 0 0 3 4 0 8 9 10
Nucleus 1 3 1 1 0 8 4 8 7 7 6 6 8 4 5 8 3 7 0 0 0 0 3 3
Nuclear Periphery 0 1 1 1 0 1 5 12 0 8 5 2 0 0 0 0 0 0 0 1 0 1 0 3
Nucleolus 0 0 0 0 1 0 1 3 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Vac/Vac Membrane 0 10 2 49 11 24 87 195 67 79 24 37 23 16 9 62 61 36 4 17 3 30 59 69
Unique Cell Count 27 158 70 193 399 421 573 727 371 537 200 340 434 639 846 264 245 168 86 201 95 248 408 582
Labelled Cell Count 28 171 74 215 414 456 622 785 419 589 227 393 472 681 912 292 263 180 86 201 95 248 408 582


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.7 6.1 6.6 7.9 8.4 8.2 8.9 8.6 9.4 8.9 9.1 8.2 8.3 8.6 11.5 11.9 12.4 8.7 8.5 9.4
Std Deviation (1e-4) 1.1 2.2 1.4 2.5 2.0 1.8 1.7 2.1 2.4 2.3 2.4 2.4 1.1 1.6 1.3 3.6 4.1 3.6 1.5 1.4 2.0
Intensity Change (Log2) 0.12 0.37 0.46 0.44 0.54 0.5 0.62 0.55 0.58 0.42 0.44 0.49 0.91 0.96 1.03 0.52 0.49 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 1.1 -1.3 -2.8 -3.8 -3.4 -3.1 -2.9 -2.6 0.5 3.4 4.4 -0.9 -1.3 -0.9
Endoplasmic Reticulum 0 -0.8 0.6 0.6 -0.4 0.4 0.7 -0.3 0.7 0 -2.6 -2.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.3 0 2.7 2.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.1 0 0 2.8 4.4 3.2 2.7 2.2 2.1 0 0 0 3.9 4.1 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0817 6.8126 6.2048 6.0653 5.161 5.9915 3.9148 4.373 3.9786 4.3129 3.9115 4.1903 2.5317 2.8673 2.9447 2.4244 2.726 2.7737
Actin 0.0021 0.0023 0.0009 0.002 0.0003 0.0005 0.0185 0.0007 0.0078 0.034 0.0016 0.0008 0.043 0.0007 0.0129 0.0108 0.0013 0.014
Bud 0.0001 0.0003 0.0001 0.0006 0 0.0001 0.0008 0.0003 0.0003 0.0007 0.0001 0.0001 0.0003 0 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0007 0.0035 0.0011 0.0036 0.001 0.003 0.0025 0.0004 0.0007 0.0081 0.002 0.002 0.001 0.0002 0.0005 0.0049 0.0067 0.0014
Bud Periphery 0.0001 0.0002 0.0001 0.0008 0 0.0001 0.0015 0.0002 0.0004 0.0007 0.0001 0.0001 0.0006 0 0.0005 0.0003 0.0001 0.0003
Bud Site 0.0017 0.0258 0.0041 0.0007 0.0001 0.0001 0.0043 0.0062 0.0016 0.0224 0.0002 0.0001 0.0017 0.0007 0.0013 0.0048 0.0006 0.0002
Cell Periphery 0.0003 0.0014 0.0004 0.0004 0.0002 0.0001 0.0009 0.0012 0.001 0.0005 0.0007 0.0002 0.0004 0.0004 0.0006 0.001 0.0002 0.0001
Cytoplasm 0.5712 0.6401 0.515 0.601 0.5684 0.6248 0.5165 0.6393 0.5161 0.5726 0.7224 0.586 0.5108 0.7376 0.6328 0.6619 0.5284 0.675
Cytoplasmic Foci 0.0063 0.0056 0.0033 0.0017 0.0028 0.0045 0.0145 0.0048 0.0265 0.0229 0.007 0.0065 0.0054 0.0028 0.0059 0.0147 0.0183 0.004
Eisosomes 0.0001 0.0001 0 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0007 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.2658 0.1971 0.3742 0.2632 0.3379 0.2515 0.2892 0.2308 0.2922 0.2147 0.1425 0.3 0.2745 0.2069 0.2895 0.2316 0.3238 0.2339
Endosome 0.0084 0.0078 0.0056 0.0039 0.0047 0.0109 0.0186 0.0058 0.0333 0.0132 0.0114 0.0137 0.0141 0.0043 0.0073 0.0084 0.0066 0.0062
Golgi 0.0007 0.0005 0.0002 0.0002 0.0001 0.0005 0.0068 0.0004 0.0099 0.004 0.0004 0.0007 0.0072 0.0003 0.0034 0.0021 0.0051 0.0038
Lipid Particles 0.0019 0.001 0.0007 0.0006 0.0011 0.0008 0.0068 0.0023 0.0106 0.0061 0.0016 0.0015 0.0259 0.0013 0.0037 0.0032 0.0274 0.0036
Mitochondria 0.0024 0.0003 0.0002 0.0002 0.0001 0.0003 0.0022 0.0001 0.0039 0.001 0.0001 0.0066 0.0061 0.0001 0.0073 0.0004 0.0339 0.0052
None 0.0718 0.0363 0.0349 0.0398 0.0166 0.04 0.0712 0.0797 0.0644 0.0397 0.077 0.0567 0.0379 0.0254 0.0073 0.0421 0.0039 0.0295
Nuclear Periphery 0.0221 0.0225 0.0255 0.0302 0.0125 0.0238 0.0155 0.0114 0.014 0.0121 0.0123 0.0096 0.0217 0.0097 0.0133 0.0063 0.0042 0.0089
Nucleolus 0.0006 0.0008 0.0003 0.0003 0.0001 0.0002 0.0004 0.0002 0.0001 0.0009 0.0003 0.0002 0.0012 0 0.0001 0.0001 0.0002 0.0001
Nucleus 0.0309 0.0392 0.0244 0.0433 0.0289 0.0314 0.011 0.0085 0.0038 0.0054 0.0111 0.0086 0.0332 0.0067 0.0044 0.0032 0.0026 0.01
Peroxisomes 0.0007 0.0002 0.0001 0 0 0.0001 0.0044 0.0001 0.0041 0.0204 0.0001 0.0001 0.0035 0 0.0026 0.0004 0.0345 0.0008
Punctate Nuclear 0.0076 0.0094 0.0053 0.0038 0.0215 0.004 0.0035 0.0015 0.0037 0.017 0.0042 0.0023 0.0053 0.0007 0.0044 0.0011 0.0004 0.0008
Vacuole 0.004 0.0053 0.003 0.0031 0.0032 0.0029 0.008 0.0057 0.0045 0.0031 0.0047 0.0036 0.0043 0.0021 0.0015 0.0018 0.0013 0.0016
Vacuole Periphery 0.0005 0.0003 0.0003 0.0003 0.0002 0.0003 0.0026 0.0002 0.0009 0.0004 0.0003 0.0005 0.0011 0.0001 0.0003 0.0002 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.8373 25.7876 23.9194 25.5927 23.376 43.2468 19.0122 22.7308 31.7684 24.4108
Translational Efficiency 1.9608 1.4327 1.0841 1.2386 1.5814 1.2065 1.6398 1.6629 0.8488 1.6151

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1540 898 1444 558 267 1466 752 1438 1807 2364 2196 1996

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 622.95 703.89 974.04 1587.85 661.77 687.77 968.76 1455.52 628.69 693.89 972.23 1492.51
Standard Deviation 128.47 125.56 239.14 333.00 220.13 172.34 259.85 378.27 146.34 156.43 246.44 370.97
Intensity Change Log 2 0.176234 0.644865 1.349886 0.055596 0.549809 1.137133 0.115353 0.596683 1.244216

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000287 0.000159 0.000789 0.005378 0.000156 0.000212 0.001552 0.002815 0.000268 0.000192 0.001051 0.003532
Bud Neck 0.009864 0.024998 0.005874 0.017432 0.006656 0.025120 0.009857 0.011859 0.009390 0.025074 0.007238 0.013417
Bud Site 0.009151 0.011671 0.010391 0.035435 0.002957 0.011879 0.020106 0.032651 0.008236 0.011800 0.013718 0.033429
Cell Periphery 0.002199 0.000169 0.001252 0.011228 0.001196 0.000671 0.001774 0.002184 0.002050 0.000480 0.001431 0.004713
Cytoplasm 0.351060* 0.341491* 0.388308* 0.312506* 0.353128* 0.346346* 0.492277* 0.410071* 0.351366* 0.344502* 0.423911* 0.382796*
Cytoplasmic Foci 0.119988 0.129305 0.015792 0.019518 0.144185 0.134906 0.039835 0.028432 0.123563 0.132778 0.024026 0.025940
Eisosomes 0.002713 0.000084 0.000100 0.000250 0.001232 0.000938 0.000119 0.000148 0.002494 0.000614 0.000106 0.000176
Endoplasmic Reticulum 0.218852* 0.001806 0.159556* 0.093467 0.121721* 0.108400* 0.098197 0.104220* 0.204500* 0.067909 0.138544* 0.101214*
Endosome 0.009783 0.048494 0.004838 0.008631 0.019528 0.031497 0.010208 0.013123 0.011223 0.037953 0.006677 0.011867
Golgi 0.009804 0.001417 0.000457 0.001774 0.002067 0.004736 0.001766 0.005651 0.008661 0.003475 0.000905 0.004567
Lipid Particles 0.052263 0.007287 0.005147 0.131139* 0.046180 0.044440 0.017129 0.037180 0.051364 0.030327 0.009250 0.063447
Mitochondria 0.010937 0.003090 0.000709 0.000356 0.000951 0.006382 0.000915 0.002248 0.009461 0.005132 0.000780 0.001719
Mitotic Spindle 0.000236 0.001911 0.001144 0.009011 0.000170 0.001685 0.009763 0.014469 0.000227 0.001771 0.004095 0.012943
None 0.045879 0.005257 0.020115 0.001826 0.040379 0.026385 0.003732 0.021245 0.045067 0.018359 0.014505 0.015816
Nuclear Periphery 0.006154 0.000911 0.012119 0.001802 0.007356 0.006141 0.004658 0.003020 0.006332 0.004154 0.009564 0.002679
Nuclear Periphery Foci 0.008053 0.001886 0.036330 0.088683 0.030975 0.006250 0.059601 0.061440 0.011440 0.004592 0.044299 0.069056
Nucleolus 0.001691 0.002607 0.000967 0.000266 0.004941 0.003009 0.000460 0.000758 0.002171 0.002856 0.000793 0.000621
Nucleus 0.098508 0.067379 0.284957* 0.074433 0.155804* 0.096823 0.094899 0.069692 0.106974 0.085638 0.219873* 0.071017
Peroxisomes 0.006624 0.004279 0.000294 0.000318 0.002062 0.004936 0.000560 0.000492 0.005950 0.004687 0.000385 0.000444
Vacuole 0.033485 0.341766* 0.050586 0.186151 0.057295 0.135966 0.132111 0.174833 0.037003 0.214142* 0.078503 0.177997
Vacuole Periphery 0.002468 0.004032 0.000275 0.000394 0.001061 0.003278 0.000483 0.003470 0.002260 0.003564 0.000346 0.002610

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.07 -4.17 -5.13 -5.26 -4.45 -0.78 -7.16 -6.68 -6.54 -2.90 1.61 -7.42 -8.24 -8.40 -5.86
Bud Neck -5.51 2.99 -6.87 1.25 -10.42 -7.81 -2.70 -4.30 5.73 -1.52 -8.96 1.10 -5.81 5.69 -9.10
Bud Site -1.21 -1.38 -7.97 -7.04 -7.27 -5.72 -8.33 -12.33 -7.68 -2.94 -2.48 -4.60 -11.96 -10.29 -8.44
Cell Periphery 7.36 3.04 -5.66 -7.80 -6.68 1.56 -1.26 -2.35 -9.80 -0.66 6.49 2.15 -4.35 -10.49 -6.79
Cytoplasm 0.78 -3.46 5.20 3.87 7.70 0.36 -6.06 -2.63 -5.03 5.23 0.81 -7.27 -1.64 -2.48 5.43
Cytoplasmic Foci -1.37 26.30 23.70 20.23 -2.93 1.15 10.93 12.81 24.18 5.18 -1.87 26.74 26.74 31.18 -0.31
Eisosomes 5.82 5.77 5.51 -5.24 -4.15 0.64 2.97 2.94 3.43 -0.74 4.53 6.12 6.00 3.12 -3.57
Endoplasmic Reticulum 28.28* 6.72 16.25 -9.36 11.04 0.71 2.49 3.40 5.40 1.54 17.32 9.15 17.77 0.31 10.88
Endosome -14.00 8.92 2.70 14.64 -4.47 -4.17 4.54 4.44 11.86 -0.24 -16.89 7.92 2.69 18.24 -4.86
Golgi 12.37 14.67 13.11 0.39 -5.00 -5.75 1.19 -1.19 1.54 -1.74 8.63 13.62 7.01 1.12 -2.78
Lipid Particles 20.51* 22.35 -7.43 -13.79 -14.06 0.24 5.47 1.52 2.19 -6.86 9.14 20.62 -2.03 -9.30 -17.42
Mitochondria 6.08 9.48 9.85 3.48 7.82 -7.90 0.79 -1.05 6.43 -1.48 4.16 9.44 8.66 6.87 -1.28
Mitotic Spindle -1.81 -3.58 -3.83 -3.02 -3.29 -2.13 -4.97 -5.78 -4.80 -0.81 -2.74 -5.82 -6.84 -5.63 -3.98
None 9.43 5.83 10.83 2.40 6.95 1.30 3.77 2.77 3.77 -4.15 6.20 7.81 8.76 3.58 1.71
Nuclear Periphery 6.73 -5.10 5.51 -4.04 12.17 0.70 1.69 2.75 3.40 2.05 2.64 -3.42 4.63 2.39 9.90
Nuclear Periphery Foci 6.58 -13.55 -14.40 -15.74 -7.52 6.04 -4.69 -4.79 -17.38 0.60 7.18 -15.77 -19.60 -23.30 -5.36
Nucleolus -2.48 3.32 7.33 6.51 5.00 1.73 3.97 3.87 7.21 -1.46 -2.07 5.61 6.96 9.47 2.75
Nucleus 5.80 -26.80 1.94 -2.73 24.70 4.73 4.81 5.72 2.69 2.01 5.00 -19.72 5.22 0.22 25.22
Peroxisomes 2.05 5.86 5.94 7.65 0.53 -3.93 5.28 5.89 7.01 1.86 1.23 6.07 6.13 9.59 0.48
Vacuole -31.29* -9.06 -23.28 5.55 -20.24 -9.39 -12.66 -22.25 -12.33 -6.87 -30.08 -17.41 -40.07 -4.10 -24.93
Vacuole Periphery -3.17 10.41 10.30 9.26 -0.36 -6.96 2.77 -1.34 1.95 -2.45 -4.58 10.65 1.56 3.99 -2.59