Standard name
Description Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0 0.07 0.08 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 1.0 0.97 0.93 0.88 0.82 0.78 0.81 0.75 0.75 0.99 0.99 0.98 0.97 0.99 0.99 0.97 0.98 0.94 0.92 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.09 0.13 0.14 0.12 0.18 0.14 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 8 12 14 8 14 27 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 3 2 3 1 6 0 0 1 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 13 3 14 17 29 21 32 26 28 53 0 1 5 4 2 1 0 0 1 0 0 0
Cytoplasm 149 98 349 263 348 365 439 389 338 270 245 339 123 221 247 215 176 178 143 98 345 73 184 157
Endoplasmic Reticulum 1 2 5 1 9 23 47 62 60 41 58 64 1 2 3 9 11 6 0 0 8 0 4 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 0 4 0 2 4 3 0 0 0 2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 2 14 11 14 27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 0 0 0 1 2 1 3 5 0 1 0 3 0 2 0 0 4 0 0 0
Unique Cell Count 150 100 359 264 357 393 501 475 431 333 326 453 124 223 251 221 178 180 148 101 367 80 199 173
Labelled Cell Count 150 101 370 267 371 411 523 495 462 362 367 525 124 225 256 233 190 187 148 101 367 80 199 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 109.1 97.1 64.0 63.7 56.5 45.3 41.0 37.2 32.8 31.0 29.3 28.4 97.3 92.5 81.6 123.7 135.3 136.4 99.3 108.8 112.1
Std Deviation (1e-4) 28.8 21.4 14.7 14.1 10.9 9.9 9.4 8.3 8.5 8.1 7.3 6.8 25.3 18.1 15.0 40.3 43.7 46.0 33.2 35.5 35.2
Intensity Change (Log2) -0.01 -0.18 -0.5 -0.64 -0.78 -0.97 -1.04 -1.13 -1.17 0.6 0.53 0.35 0.95 1.08 1.09 0.63 0.77 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.0 3.4 0 4.0 4.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 0.2 0.5 1.5 0.6 2.3 2.4 2.7 4.2 0 -2.4 -1.2 -1.3 0 0
Cytoplasm 2.3 0.2 -2.7 -5.0 -6.9 -7.8 -6.9 -8.5 -8.8 1.3 1.6 1.0 0.1 1.2 1.2
Endoplasmic Reticulum 0 1.1 3.2 4.8 6.1 6.4 5.8 7.4 6.5 0 0 0 2.0 3.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.4 3.5 4.0 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc60-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available