Standard name
Human Ortholog
Description GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.18 0 0 0 0 0 0 0 0 0 0 0.06 0 0.11 0.05 0.19 0 0.07 0.07 0.09 0.08 0.1
Bud 0 0.07 0.05 0 0.08 0 0.05 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.34 0.13 0.57 0.44 0.27 0.3 0.29 0.22 0.26 0.29 0.25 0.58 0.61 0.51 0.29 0.27 0.24 0.06 0.13 0.08 0.22 0.27 0.19
Endoplasmic Reticulum 0 0.07 0.05 0 0 0 0 0.06 0.09 0.05 0 0 0.11 0.07 0.06 0.05 0 0.07 0 0 0 0 0.06 0.11
Endosome 0.53 0.29 0.08 0.05 0.29 0.17 0.09 0.13 0 0 0 0 0.22 0.2 0.29 0.37 0.44 0.27 0.42 0.38 0.39 0.31 0.28 0.23
Golgi 0 0.21 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.08 0.24 0.28 0.19 0.13 0.12 0.13 0.15
Mitochondria 0.35 0.21 0.64 0.41 0.27 0.64 0.58 0.56 0.76 0.74 0.8 0.79 0.05 0.06 0.05 0.14 0.12 0.11 0.07 0 0.13 0.11 0.08 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 0.05 0 0.08 0 0 0.07 0 0 0 0 0 0 0 0.19 0.15 0.07 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 4 7 2 2 3 0 5 5 4 3 1 8 22 14 24 8 14 21 7 6 24 37 56
Bud 15 4 2 2 4 3 8 12 9 9 6 11 3 13 1 4 3 2 2 3 0 3 2 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 0 2 6 8
Bud Site 0 0 1 0 0 0 1 1 1 2 0 1 0 0 0 0 0 0
Cell Periphery 2 1 0 0 0 0 1 0 2 1 1 3 1 4 1 3 0 1 0 0 0 0 0 1
Cytoplasm 78 20 5 35 23 26 51 60 49 53 41 51 113 232 189 64 41 18 26 13 6 63 122 113
Endoplasmic Reticulum 5 4 2 2 0 4 4 12 19 10 5 9 22 25 24 11 4 5 9 1 2 11 27 64
Endosome 220 17 3 3 15 16 16 26 7 6 4 7 44 77 107 82 68 20 199 40 32 87 129 131
Golgi 7 12 1 0 0 2 1 0 2 1 0 1 5 14 29 12 13 18 132 20 10 34 57 86
Mitochondria 146 12 25 25 14 61 99 115 169 153 113 161 9 21 17 32 18 8 35 4 11 30 34 48
Nucleus 5 1 0 0 0 0 1 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 2
Nucleolus 7 0 0 1 0 0 0 1 0 2 0 1 0 0 0 1 0 1 0 0 0 0 0 1
Peroxisomes 16 0 0 0 0 2 2 8 0 2 0 2 0 3 1 0 2 0 11 1 3 2 2 4
SpindlePole 9 0 2 0 4 1 6 14 5 7 0 1 0 5 6 42 23 5 12 3 4 7 10 13
Vac/Vac Membrane 11 3 1 1 1 1 5 7 1 7 1 4 3 10 16 8 13 4 10 7 2 10 19 32
Unique Cell Count 415 58 39 61 52 96 172 206 222 207 142 204 196 381 372 223 154 74 475 107 84 285 461 585
Labelled Cell Count 531 78 49 71 63 120 195 262 269 257 174 253 208 428 406 283 195 96 475 107 84 285 461 585


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.8 5.9 5.5 6.0 5.0 4.7 4.9 4.5 4.5 4.4 4.3 7.9 7.7 7.8 7.1 8.5 9.7 7.7 7.7 8.5
Std Deviation (1e-4) 0.9 1.1 0.8 0.7 1.3 0.7 1.0 1.2 1.0 1.1 1.2 1.0 1.2 1.0 1.1 1.0 1.4 1.1 1.3 1.3 1.4
Intensity Change (Log2) -0.11 0.0 -0.25 -0.35 -0.29 -0.39 -0.41 -0.42 -0.46 0.42 0.38 0.39 0.27 0.51 0.71 0.37 0.37 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.4 3.2 1.8 2.1 2.1 1.3 1.7 2.0 1.7 5.1 5.8 4.5 2.1 1.8 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.5 1.4 0 0 0 0 0 0 2.1 1.9 2.8 3.6 4.2 2.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9
Mitochondria -2.3 -3.5 -0.1 0 0 0 0 0 0 -9.6 0 0 -6.9 -7.0 -5.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.1 1.6 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1235 3.2517 2.9291 1.8638 2.2978 2.8446 2.1094 2.9468 2.3988 1.832 1.6282 2.2448 3.8212 4.8622 4.1459 4.1092 3.6625 3.9389
Actin 0.1892 0.0255 0.0326 0.0006 0.0057 0.0155 0.0309 0.0249 0.0054 0.0062 0.0011 0.0038 0.0691 0.0137 0.0146 0.0222 0.0063 0.0149
Bud 0.0004 0.001 0.0005 0.0006 0.0004 0.0005 0.0004 0.0021 0.0004 0.0001 0.0002 0.0002 0.0006 0.001 0.0005 0.0027 0.0003 0.0003
Bud Neck 0.0007 0.0024 0.0005 0.001 0.0014 0.009 0.0007 0.0018 0.0005 0.0004 0.0005 0.002 0.0008 0.0007 0.0009 0.0012 0.0004 0.0017
Bud Periphery 0.0003 0.0005 0.0007 0.0004 0.0007 0.0003 0.0004 0.0022 0.0003 0 0.0001 0.0001 0.0006 0.0003 0.0002 0.0014 0.001 0.0001
Bud Site 0.0013 0.0084 0.0017 0.0027 0.0042 0.0007 0.0011 0.0494 0.0044 0.0003 0.0005 0.0008 0.0029 0.0091 0.003 0.001 0.0004 0.0004
Cell Periphery 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0006 0.0003 0 0 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.1148 0.0978 0.1734 0.1009 0.007 0.1419 0.0928 0.0992 0.0705 0.1116 0.0327 0.1232 0.0459 0.1382 0.0949 0.0799 0.0055 0.1139
Cytoplasmic Foci 0.1779 0.2864 0.1863 0.2785 0.2999 0.2822 0.2792 0.337 0.3275 0.3249 0.3732 0.3492 0.2954 0.3216 0.3124 0.2701 0.3717 0.3119
Eisosomes 0.0002 0.0001 0.0004 0 0.0002 0 0.0003 0.0001 0.0004 0 0 0 0.0003 0.0002 0 0.0001 0 0
Endoplasmic Reticulum 0.0037 0.0057 0.0087 0.0034 0.0005 0.0043 0.0083 0.0019 0.0016 0.0006 0.0005 0.0027 0.0062 0.004 0.0024 0.0016 0.0011 0.0024
Endosome 0.1989 0.2581 0.3504 0.3119 0.2838 0.2258 0.319 0.2278 0.2884 0.2921 0.399 0.3313 0.3085 0.2712 0.3237 0.3296 0.3386 0.2792
Golgi 0.1471 0.1628 0.1147 0.1018 0.0666 0.1596 0.0882 0.1745 0.1401 0.119 0.1504 0.1413 0.1071 0.1568 0.1693 0.137 0.174 0.1695
Lipid Particles 0.01 0.0153 0.0142 0.0114 0.0545 0.0222 0.0152 0.0046 0.0253 0.0036 0.0157 0.0037 0.0237 0.0098 0.0069 0.0062 0.0154 0.0138
Mitochondria 0.0048 0.0052 0.0102 0.0027 0.0457 0.0142 0.0084 0.0052 0.007 0.001 0.003 0.0037 0.0099 0.0061 0.007 0.0027 0.0239 0.0113
None 0.0501 0.0572 0.0085 0.0038 0.0003 0.0192 0.0942 0.0095 0.0527 0.0044 0.0021 0.0018 0.0361 0.0181 0.0101 0.064 0.0006 0.0119
Nuclear Periphery 0.0179 0.0104 0.004 0.0074 0.001 0.0072 0.0033 0.0003 0.0004 0.0001 0.0001 0.0003 0.0081 0.0008 0.0002 0.0003 0.0003 0.0002
Nucleolus 0.0001 0.0003 0.0002 0.0023 0.0017 0.0005 0.0011 0.0001 0.0002 0 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0001 0
Nucleus 0.0532 0.0218 0.0134 0.13 0.1988 0.0143 0.0024 0.0004 0.0003 0.0001 0.0001 0.0002 0.0015 0.0002 0.0001 0.0011 0.0001 0.0002
Peroxisomes 0.0244 0.0309 0.0273 0.0219 0.0202 0.071 0.042 0.0521 0.0691 0.1337 0.0179 0.031 0.0749 0.042 0.05 0.0728 0.0559 0.0637
Punctate Nuclear 0.0021 0.0036 0.0432 0.0045 0.0025 0.0041 0.0069 0.0004 0.0013 0.0004 0.0008 0.0004 0.0035 0.001 0.0006 0.0005 0.0005 0.0011
Vacuole 0.0018 0.0053 0.0064 0.0115 0.003 0.0047 0.0037 0.0046 0.0031 0.0011 0.0012 0.0029 0.0028 0.003 0.0018 0.0041 0.0025 0.0023
Vacuole Periphery 0.001 0.0011 0.0025 0.0026 0.0017 0.0025 0.0015 0.001 0.001 0.0005 0.0008 0.0013 0.0015 0.0016 0.001 0.0014 0.0013 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.821 19.3407 27.1087 40.6747 28.0512 25.6277 24.8064 31.0411 41.3372 34.976
Translational Efficiency 0.7351 0.5422 0.7379 0.529 0.4084 0.4714 0.5217 0.5817 0.4573 0.4201

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
306 1312 1604 1031 1542 1034 1871 130 1848 2346 3475 1161

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 798.68 930.56 948.63 968.45 912.91 863.29 929.42 967.04 894.00 900.91 938.29 968.29
Standard Deviation 110.38 140.30 112.79 123.58 125.03 118.33 103.74 139.21 129.86 135.26 108.43 125.43
Intensity Change Log 2 0.220482 0.248228 0.278060 -0.080627 0.025858 0.083103 0.067722 0.133898 0.177362

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002004 0.001242 0.009742 0.012471 0.001783 0.002284 0.003440 0.007551 0.001819 0.001701 0.006349 0.011920
Bud Neck 0.004061 0.001200 0.002580 0.007504 0.001505 0.002358 0.008159 0.008708 0.001928 0.001710 0.005584 0.007639
Bud Site 0.020027 0.011029 0.030620 0.063535 0.014771 0.015157 0.013903 0.087445 0.015641 0.012848 0.021619 0.066212
Cell Periphery 0.000105 0.000043 0.000054 0.000115 0.000077 0.000129 0.000039 0.001020 0.000082 0.000081 0.000046 0.000216
Cytoplasm 0.027801 0.026798 0.078184 0.135625* 0.049911 0.029450 0.049491 0.138964* 0.046250 0.027967 0.062736 0.135999*
Cytoplasmic Foci 0.431080* 0.449053* 0.437451* 0.324146* 0.453360* 0.409734* 0.347757* 0.266613* 0.449671* 0.431723* 0.389158* 0.317704*
Eisosomes 0.000085 0.000032 0.000043 0.000068 0.000058 0.000054 0.000053 0.000069 0.000063 0.000042 0.000048 0.000068
Endoplasmic Reticulum 0.002808 0.000326 0.000915 0.000705 0.001203 0.000664 0.000699 0.000791 0.001469 0.000475 0.000799 0.000715
Endosome 0.103655 0.135231 0.131178 0.089970 0.111467 0.170814 0.316398* 0.111619 0.110174 0.150915 0.230903* 0.092394
Golgi 0.316224* 0.286257* 0.107045 0.023661 0.292465* 0.299272* 0.052873 0.025392 0.296399* 0.291994* 0.077878 0.023855
Lipid Particles 0.009072 0.003046 0.010039 0.006469 0.006134 0.004549 0.014588 0.005806 0.006621 0.003708 0.012488 0.006395
Mitochondria 0.014478 0.007663 0.001497 0.003277 0.008490 0.009350 0.003380 0.001947 0.009481 0.008407 0.002511 0.003129
Mitotic Spindle 0.021158 0.043258 0.109334* 0.080523 0.028972 0.020347 0.047489 0.050194 0.027678 0.033160 0.076036 0.077127
None 0.000142 0.000509 0.000045 0.000110 0.000204 0.000058 0.000058 0.000568 0.000194 0.000310 0.000052 0.000162
Nuclear Periphery 0.000070 0.000047 0.000146 0.000194 0.000101 0.000023 0.000431 0.000234 0.000096 0.000037 0.000299 0.000199
Nuclear Periphery Foci 0.000861 0.000618 0.003123 0.001166 0.001128 0.000257 0.001821 0.001188 0.001084 0.000459 0.002422 0.001168
Nucleolus 0.000216 0.000364 0.000085 0.000381 0.000180 0.000102 0.001174 0.000305 0.000186 0.000249 0.000672 0.000372
Nucleus 0.002024 0.000825 0.001299 0.004350 0.000523 0.000243 0.003553 0.013703 0.000771 0.000568 0.002513 0.005397
Peroxisomes 0.036362 0.018011 0.039555 0.131239* 0.010293 0.022280 0.041145 0.118518* 0.014610 0.019893 0.040411 0.129815*
Vacuole 0.004459 0.009844 0.035443 0.112644 0.010273 0.008158 0.091200 0.157233 0.009311 0.009101 0.065464 0.117637
Vacuole Periphery 0.003306 0.004604 0.001622 0.001847 0.007103 0.004715 0.002348 0.002132 0.006474 0.004653 0.002013 0.001879

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.40 -7.55 -5.05 -5.88 0.30 -0.93 -2.67 -2.36 -2.00 -1.34 0.34 -8.02 -5.69 -5.80 -1.94
Bud Neck 3.93 1.63 -4.65 -14.00 -10.24 -2.29 -10.81 -5.88 -4.91 0.15 0.89 -10.11 -13.59 -13.69 -3.96
Bud Site 2.21 -2.66 -6.98 -13.05 -6.52 -0.19 0.35 -5.62 -5.57 -5.67 1.79 -4.15 -12.54 -13.70 -10.93
Cell Periphery 2.57 2.08 0.26 -2.15 -1.67 -0.68 3.41 -4.22 -3.90 -4.35 0.02 3.49 -3.60 -2.85 -4.56
Cytoplasm 0.17 -9.35 -16.87 -19.44 -9.84 4.88 -0.89 -6.10 -7.18 -5.92 5.55 -6.07 -17.25 -20.88 -14.18
Cytoplasmic Foci -1.56 0.52 13.07 21.42 18.16 5.80 16.42 13.09 10.48 6.78 3.09 12.23 24.35 22.92 15.97
Eisosomes 4.73 3.82 2.17 -5.00 -3.40 0.41 0.51 -0.72 -1.58 -1.79 2.31 1.57 0.16 -3.97 -2.76
Endoplasmic Reticulum 1.32 0.95 1.10 -2.97 1.36 1.60 1.69 1.26 -0.34 -0.21 2.51 1.54 1.80 -1.82 0.70
Endosome -3.70 -2.73 3.93 12.64 11.63 -9.26 -29.13* 2.29 6.61 16.56 -9.11 -23.11 8.44 17.07 30.06*
Golgi 2.04 15.50 22.19 43.04* 22.13* -0.75 38.82* 35.76* 32.40 6.08 0.64 38.85 50.30* 56.62* 22.37*
Lipid Particles 2.73 -0.72 1.21 -3.77 4.54 1.55 -5.69 0.75 -0.24 4.83 3.75 -6.14 0.49 -3.18 6.53
Mitochondria 2.19 4.48 4.06 3.88 -0.94 -0.49 5.34 5.81 4.62 2.07 0.84 7.61 6.27 5.32 0.36
Mitotic Spindle -4.75 -14.79 -9.38 -6.14 3.28 2.33 -5.73 -2.33 -2.86 -0.81 -1.84 -14.27 -9.30 -8.26 -0.54
None -1.14 3.10 -1.33 0.82 -2.76 4.47 3.77 -2.56 -3.41 -3.30 -0.64 4.69 -1.37 0.12 -3.48
Nuclear Periphery 0.82 -2.60 -6.71 -10.67 -5.65 2.20 -3.24 -4.37 -6.30 0.69 1.95 -3.67 -7.54 -12.91 -0.58
Nuclear Periphery Foci 0.31 -3.84 -3.30 -3.59 1.96 3.63 -2.49 -1.74 -4.72 0.68 2.06 -4.34 -3.44 -5.31 1.41
Nucleolus -0.60 1.49 -2.52 -0.94 -3.66 1.16 -3.66 -1.86 -2.73 3.00 -0.50 -3.37 -3.10 -1.90 0.98
Nucleus 1.06 0.34 -2.98 -5.30 -4.72 3.29 -5.33 -4.01 -4.05 -3.47 0.74 -4.90 -6.73 -6.90 -5.16
Peroxisomes 4.19 -0.52 -9.39 -14.94 -10.62 -5.78 -13.97 -5.12 -4.23 -2.92 -3.32 -13.90 -16.58 -15.41 -11.22
Vacuole -4.39 -15.90 -24.70 -24.05 -18.90 1.89 -22.58 -8.91 -9.00 -5.02 0.26 -26.05 -25.75 -25.80 -16.13
Vacuole Periphery -1.75 2.66 2.76 6.67 0.72 2.82 8.03 7.61 4.08 1.29 3.02 8.92 8.88 7.33 1.87