ORF
Human Ortholog
Description Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.14 0.05 0 0.08 0 0.08 0.05 0 0.07 0.07 0.14 0.12 0.07 0.12 0.09 0.11 0.22 0.17 0.12 0.14 0.19 0.19
Bud 0 0.05 0 0 0 0 0.05 0 0.05 0.08 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0.08 0.08 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.19 0.21 0.2 0.22 0.15 0.17 0.13 0.14 0.13 0.15 0.18 0.31 0.31 0.26 0.11 0.15 0.14 0.42 0.43 0.45 0.57 0.53 0.55
Cytoplasm 0.85 0.79 0.88 0.9 0.84 0.87 0.83 0.66 0.64 0.52 0.59 0.81 0.83 0.89 0.78 0.81 0.79 0.15 0.16 0.17 0.07 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.09 0 0.06 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.14 0.3 0.35 0.42 0.35 0 0 0 0 0 0 0 0.06 0.11 0.08 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 58 16 6 28 10 31 17 15 20 20 60 65 41 46 26 23 86 73 44 54 119 119
Bud 10 20 7 1 10 7 19 11 16 24 21 31 19 21 4 7 2 3 0 0 3 8 9
Bud Neck 4 4 15 3 10 1 2 2 3 1 0 1 4 2 5 4 0 22 29 24 31 47 39
Bud Site 0 0 1 0 0 1 1 1 0 2 2 0 0 0 0 0 0
Cell Periphery 70 85 70 55 52 50 53 44 45 42 51 137 174 162 42 42 29 166 183 173 230 336 357
Cytoplasm 316 320 305 228 292 259 330 208 215 147 168 355 467 554 291 225 164 61 66 64 27 37 31
Endoplasmic Reticulum 8 7 4 0 4 1 2 5 2 13 5 0 1 2 32 12 12 19 14 13 8 16 19
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 8 5 1 3 5 5
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 4 0 0 2 2
Mitochondria 5 5 0 2 10 44 56 94 118 120 100 4 0 3 6 4 5 16 25 42 30 39 38
Nucleus 0 0 0 0 2 0 1 1 3 4 1 1 0 1 5 1 2 0 2 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 1
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 0 0 0 1 1 1 1 0 10 18 0 0 0 0 0 0 0 0 1
SpindlePole 4 4 3 2 7 3 5 4 1 2 7 3 15 3 15 9 9 1 1 1 0 0 3
Vac/Vac Membrane 0 1 0 0 1 1 0 0 1 0 0 0 0 1 3 4 2 4 10 6 6 6 3
Unique Cell Count 370 404 345 253 348 299 397 314 336 283 283 438 562 622 375 278 207 400 428 385 404 629 645
Labelled Cell Count 447 505 422 297 416 378 500 388 420 377 376 592 755 809 452 335 248 400 428 385 404 629 645


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.4 5.5 5.3 5.5 4.5 4.7 4.3 4.1 3.8 4.4 4.9 5.1 5.2 6.7 7.1 6.2 4.5 4.6 5.1
Std Deviation (1e-4) 1.0 0.8 0.8 1.0 1.3 1.1 1.0 0.9 1.0 0.8 1.1 0.9 1.2 1.2 1.2 1.6 1.2 1.0 1.3 1.5
Intensity Change (Log2) -0.05 0.0 -0.28 -0.23 -0.36 -0.42 -0.55 -0.32 -0.18 -0.1 -0.07 0.29 0.38 0.18 -0.29 -0.26 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 1.8 0 0 0 0 0 0 0 3.6 1.2 3.6 2.3 2.9
Bud 0 0.7 0 0 0 0 0 0 0 1.2 1.2 -1.1 0.4 0
Bud Neck -2.2 -1.0 0 0 0 0 0 0 0 -3.7 -4.6 -2.5 -2.1 -3.0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -1.8 0 0 0 0 0 0 0 3.5 2.0 -3.4 -1.7 -1.9
Cytoplasm 0.7 -1.7 -0.7 -2.0 -6.8 -7.5 -10.1 -8.4 -2.8 -2.2 0.3 -3.8 -2.6 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 4.5 2.5 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1.9 0 2.7 2.1 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5267 2.9713 2.6558 2.4211 2.1536 2.7935 0.0158 1.4581 1.2229 1.3613 0.952 0.7899 0.8092 1.0059 0.8444 1.0189 0.9905 0.4962
Actin 0.0516 0.0082 0.03 0.0069 0.065 0.0251 0.0851 0.049 0.0676 0.1114 0.0476 0.0305 0.0356 0.0122 0.0353 0.0072 0.0331 0.0187
Bud 0.0021 0.0012 0.002 0.0008 0.0043 0.0013 0.0009 0.0008 0.0085 0.0059 0.0159 0.001 0.0105 0.0123 0.015 0.0249 0.0139 0.024
Bud Neck 0.0162 0.0053 0.0254 0.0204 0.0288 0.0375 0.012 0.0091 0.0331 0.0174 0.0075 0.0624 0.0053 0.0044 0.0099 0.0076 0.0034 0.017
Bud Periphery 0.0088 0.0058 0.0159 0.0086 0.0205 0.0117 0.0051 0.0029 0.0106 0.0146 0.0221 0.0114 0.0159 0.0056 0.0177 0.0272 0.0156 0.0295
Bud Site 0.0349 0.129 0.0834 0.007 0.0184 0.0028 0.0286 0.1211 0.1158 0.0158 0.0082 0.005 0.0299 0.1316 0.074 0.0386 0.0131 0.0063
Cell Periphery 0.1345 0.1342 0.1169 0.1754 0.1721 0.126 0.0937 0.1245 0.1272 0.0463 0.0212 0.162 0.0635 0.0707 0.0722 0.0478 0.053 0.1078
Cytoplasm 0.3156 0.4798 0.4026 0.4726 0.4382 0.5311 0.3658 0.5229 0.3831 0.4737 0.3574 0.6106 0.2864 0.4981 0.4081 0.4687 0.4869 0.49
Cytoplasmic Foci 0.0169 0.0036 0.0071 0.0041 0.0075 0.0071 0.0349 0.0085 0.0194 0.0483 0.0128 0.0105 0.0416 0.0115 0.0178 0.026 0.0116 0.0146
Eisosomes 0.0085 0.0074 0.0106 0.0035 0.0043 0.005 0.0121 0.0184 0.0074 0.0027 0.0024 0.0029 0.0057 0.0042 0.0058 0.0018 0.001 0.0031
Endoplasmic Reticulum 0.0075 0.0044 0.0051 0.0058 0.0021 0.0039 0.007 0.0046 0.0044 0.007 0.0052 0.0077 0.0136 0.0072 0.0049 0.0113 0.0032 0.0045
Endosome 0.0246 0.0006 0.001 0.0005 0.0069 0.0021 0.0177 0.001 0.0099 0.0224 0.0241 0.002 0.0269 0.0027 0.0114 0.0358 0.0129 0.0036
Golgi 0.0062 0.0009 0.0023 0.0006 0.0081 0.0032 0.0123 0.0029 0.0064 0.0056 0.0046 0.0017 0.0118 0.0045 0.0085 0.0127 0.0124 0.0033
Lipid Particles 0.0088 0.001 0.0027 0.0015 0.0048 0.0053 0.0277 0.0019 0.017 0.0037 0.0071 0.0023 0.0199 0.0035 0.0043 0.0089 0.0162 0.0053
Mitochondria 0.0076 0.0004 0.0062 0.0005 0.0284 0.0029 0.0296 0.0088 0.0069 0.0134 0.0082 0.0003 0.026 0.0012 0.0059 0.0108 0.0032 0.0011
None 0.3161 0.2144 0.2739 0.2816 0.1745 0.2292 0.2265 0.1194 0.1631 0.1832 0.3951 0.0858 0.3304 0.219 0.2964 0.2298 0.3038 0.2384
Nuclear Periphery 0.0137 0.0003 0.0002 0.0006 0.0013 0.0003 0.0022 0.0003 0.0018 0.0076 0.0279 0.0002 0.0151 0.0005 0.001 0.004 0.0016 0.0009
Nucleolus 0.0029 0.0004 0.0004 0.0009 0.0009 0.0004 0.0031 0.0003 0.0028 0.0018 0.0039 0.0004 0.0062 0.0007 0.0013 0.001 0.0014 0.0009
Nucleus 0.0047 0.0008 0.0006 0.001 0.0011 0.0006 0.0032 0.0006 0.0029 0.0046 0.0111 0.0006 0.0164 0.0053 0.0024 0.0031 0.0026 0.0063
Peroxisomes 0.0064 0.0002 0.0118 0.0004 0.007 0.0014 0.0191 0.0011 0.0057 0.0077 0.0016 0.0002 0.0085 0.0009 0.0025 0.0046 0.0049 0.0131
Punctate Nuclear 0.0045 0.0003 0.0005 0.0047 0.0039 0.0011 0.0098 0.0004 0.0027 0.004 0.0054 0.0006 0.019 0.0021 0.0017 0.0224 0.0032 0.004
Vacuole 0.0059 0.0016 0.0013 0.0025 0.0015 0.0019 0.0028 0.0013 0.0031 0.0023 0.0061 0.0017 0.008 0.0017 0.0035 0.0044 0.0024 0.0072
Vacuole Periphery 0.0021 0.0001 0.0001 0.0001 0.0004 0.0002 0.0008 0.0001 0.0005 0.0008 0.0048 0.0001 0.004 0.0001 0.0005 0.0014 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.2329 5.8322 8.6555 11.3748 10.3093 9.7809 11.9782 12.562 12.0109 13.3757
Translational Efficiency 0.5525 0.9438 0.6767 0.5682 0.4639 0.6253 0.6236 0.5021 0.6074 0.5497

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2409 2107 565 1606 1121 2220 2515 1261 3530 4327 3080 2867

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.46 789.51 972.11 860.55 651.95 771.53 859.50 802.18 676.87 780.29 880.16 834.88
Standard Deviation 70.05 91.31 102.44 122.59 98.76 110.62 92.16 126.42 82.07 102.07 103.73 127.62
Intensity Change Log 2 0.197585 0.497747 0.321886 0.242961 0.398736 0.299165 0.219833 0.450438 0.310880

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.015262 0.020506 0.006145 0.026684 0.011040 0.011902 0.001200 0.032458 0.013921 0.016092 0.002107 0.029223
Bud Neck 0.057622 0.053165 0.017270 0.028383 0.039613 0.078033 0.016344 0.022533 0.051903 0.065924 0.016514 0.025810
Bud Site 0.020650 0.037447 0.043091 0.100317* 0.017068 0.039288 0.019358 0.107908* 0.019512 0.038392 0.023712 0.103656*
Cell Periphery 0.029931 0.010643 0.007935 0.017518 0.030462 0.007170 0.003045 0.021727 0.030100 0.008861 0.003942 0.019370
Cytoplasm 0.514181* 0.519410* 0.194832* 0.334123* 0.509452* 0.490170* 0.456573* 0.380962* 0.512679* 0.504408* 0.408559* 0.354724*
Cytoplasmic Foci 0.049873 0.120075 0.060109 0.032817 0.108291 0.127117 0.027858 0.058651 0.068424 0.123688 0.033774 0.044180
Eisosomes 0.142043* 0.037912 0.002736 0.011488 0.073902 0.028472 0.001365 0.024408 0.120403* 0.033068 0.001617 0.017170
Endoplasmic Reticulum 0.003322 0.002686 0.037463 0.012834 0.005506 0.002352 0.002434 0.010736 0.004016 0.002514 0.008860 0.011911
Endosome 0.002044 0.005433 0.006306 0.003523 0.007315 0.010428 0.001375 0.002841 0.003718 0.007996 0.002280 0.003223
Golgi 0.013846 0.014375 0.000640 0.003667 0.019838 0.017287 0.000163 0.000944 0.015749 0.015869 0.000250 0.002470
Lipid Particles 0.015089 0.009517 0.012257 0.003892 0.050828 0.014729 0.002111 0.006839 0.026439 0.012191 0.003972 0.005188
Mitochondria 0.006515 0.007171 0.000557 0.002140 0.008017 0.009502 0.000555 0.001034 0.006992 0.008367 0.000556 0.001653
Mitotic Spindle 0.000262 0.001928 0.002416 0.009600 0.000652 0.001852 0.002274 0.002510 0.000386 0.001889 0.002300 0.006481
None 0.005286 0.001588 0.000703 0.001001 0.007841 0.001437 0.001612 0.004013 0.006098 0.001511 0.001445 0.002326
Nuclear Periphery 0.000103 0.000091 0.001449 0.000356 0.000371 0.000142 0.000528 0.000272 0.000188 0.000117 0.000697 0.000319
Nuclear Periphery Foci 0.000222 0.000401 0.006478 0.001523 0.000731 0.000545 0.000559 0.001617 0.000384 0.000475 0.001645 0.001565
Nucleolus 0.000280 0.000242 0.000389 0.000108 0.000646 0.000433 0.000140 0.000169 0.000397 0.000340 0.000186 0.000135
Nucleus 0.089601 0.099654 0.408892* 0.250079* 0.075517 0.094870 0.399590* 0.170947* 0.085129 0.097200 0.401297* 0.215274*
Peroxisomes 0.020710 0.018417 0.001672 0.003066 0.017056 0.018520 0.001172 0.006674 0.019550 0.018470 0.001264 0.004653
Vacuole 0.012930 0.038866 0.188515 0.155898 0.015219 0.044753 0.061621 0.142543 0.013657 0.041887 0.084899 0.150024
Vacuole Periphery 0.000228 0.000470 0.000147 0.000980 0.000635 0.000997 0.000122 0.000211 0.000357 0.000740 0.000126 0.000642

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.15 7.19 -3.85 -1.36 -8.37 -0.61 8.42 -6.93 -6.93 -11.75 -1.97 14.66 -7.22 -5.97 -15.74
Bud Neck 1.59 16.55 12.83 11.07 -5.08 -10.49 9.10 6.94 18.97 -3.18 -5.85 19.63 15.14 21.44 -6.42
Bud Site -7.41 -4.26 -15.03 -11.08 -7.51 -8.69 -0.75 -14.02 -9.90 -13.61 -10.88 -1.80 -20.36 -14.78 -18.43
Cell Periphery 13.93 16.84 9.67 -3.43 -6.30 9.63 11.65 4.09 -7.87 -11.30 17.89 23.57 9.60 -8.28 -15.00
Cytoplasm -0.75 40.65 27.74 26.62 -14.82 2.24 7.90 14.66 14.59 9.05 1.70 20.17 30.24 29.05 8.76
Cytoplasmic Foci -19.93 -2.06 7.44 24.80 6.76 -3.69 19.30 10.15 16.14 -9.50 -19.72 16.17 10.33 29.38 -4.88
Eisosomes 27.01* 40.62* 37.56* 13.93 -7.27 11.17 18.87 12.08 2.96 -9.56 30.10* 44.24* 36.74 11.96 -12.47
Endoplasmic Reticulum 1.91 -14.23 -10.91 -11.73 9.73 3.76 3.31 -4.48 -9.32 -8.99 4.27 -8.57 -11.78 -14.98 -3.33
Endosome -8.61 -10.68 -2.02 3.78 5.72 -3.07 7.80 5.41 9.93 -4.35 -9.08 4.14 1.61 9.84 -1.83
Golgi -0.51 21.75 9.77 9.65 -2.47 1.71 16.96 16.34 16.69 -9.77 -0.09 27.96 17.94 17.09 -3.37
Lipid Particles 6.47 2.63 15.38 10.24 12.69 9.11 12.45 11.08 7.63 -5.84 9.99 16.30 15.11 12.32 -2.84
Mitochondria -0.74 12.81 7.36 6.25 -2.60 -1.06 7.19 6.47 8.75 -1.02 -1.83 14.17 10.31 10.54 -2.79
Mitotic Spindle -2.73 -1.41 -4.46 -3.31 -2.63 -2.02 -2.24 -2.34 -1.16 -0.76 -3.72 -3.22 -5.10 -3.50 -2.99
None 5.58 9.93 9.21 1.39 -1.57 3.77 3.64 2.22 -2.29 -2.06 6.84 6.65 5.39 -1.17 -1.35
Nuclear Periphery 0.85 -21.14 -12.84 -11.93 15.45 3.80 -3.95 0.62 -6.88 10.03 3.16 -21.14 -8.26 -13.17 14.54
Nuclear Periphery Foci -1.95 -14.94 -15.65 -10.10 11.24 0.19 0.88 -7.22 -4.22 -9.19 -1.16 -13.68 -16.93 -7.99 -0.15
Nucleolus 0.69 -1.44 7.97 2.57 3.82 1.54 4.85 4.35 3.23 -1.34 0.83 5.40 6.73 4.24 2.25
Nucleus -2.97 -26.70 -26.60 -23.90 11.90 -5.00 -54.26 -15.23 -12.45 31.21 -4.86 -62.73 -29.56 -26.37 30.43
Peroxisomes 1.75 20.87 18.78 15.43 -2.54 -1.08 14.61 7.43 9.55 -5.30 1.15 25.16 18.12 18.04 -6.02
Vacuole -18.94 -23.77 -33.34 -27.97 1.75 -15.40 -22.73 -25.44 -20.49 -15.08 -24.26 -31.35 -41.80 -34.23 -17.52
Vacuole Periphery -5.54 3.32 -1.91 -1.11 -2.15 -2.13 5.57 3.94 5.10 -1.24 -4.66 6.37 -1.05 1.06 -2.41