Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.99 0.93 0.78 0.74 0.73 0.65 0.58 0.5 0.43 1.0 1.0 1.0 0.97 0.98 0.94 0.85 0.91 0.85 0.92 0.91 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0.11 0.41 0.43 0.42 0.63 0.69 0.7 0.74 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 3
Bud 2 0 3 2 0 5 4 7 5 7 4 11 0 0 0 0 1 0 1 1 3 0 1 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 1 2
Bud Site 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 4 0 2 2 4 4 6 2 7 1 1 1 2 6 2 0 1 0 0 0 0
Cytoplasm 90 317 180 147 192 241 295 367 208 211 119 153 214 339 306 201 119 109 83 297 163 121 193 163
Endoplasmic Reticulum 0 5 3 0 1 0 1 4 0 3 2 3 1 1 0 9 3 7 0 0 1 0 1 2
Endosome 0 0 0 0 3 4 4 9 0 1 2 1 0 0 1 1 3 3 1 3 3 1 0 1
Golgi 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 1 2
Mitochondria 10 12 6 6 23 125 173 212 200 250 167 262 0 0 0 5 1 2 4 8 8 3 4 2
Nucleus 0 0 0 0 1 2 1 5 1 0 3 1 0 0 0 2 1 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 7 4 1 2 1 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 0 0 2 2 5 8 9 6 2 4 8 0 0 0 2 1 0 4 3 1 0 1 10
Unique Cell Count 93 328 187 148 206 308 400 503 318 361 238 354 215 340 307 207 122 116 98 327 193 133 212 201
Labelled Cell Count 103 338 196 161 223 385 492 626 430 482 306 448 216 341 308 222 135 125 98 327 193 133 212 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.4 4.8 4.8 4.4 3.8 3.2 3.9 3.5 3.3 3.5 3.3 5.0 4.9 5.0 5.2 6.1 6.2 4.6 4.7 5.4
Std Deviation (1e-4) 0.9 0.7 1.4 1.8 1.6 1.6 0.8 1.4 1.3 1.1 1.0 1.1 1.2 1.5 1.4 1.2 1.4 1.2 1.4 1.1 1.7
Intensity Change (Log2) – – – -0.02 -0.14 -0.34 -0.6 -0.3 -0.46 -0.54 -0.47 -0.56 0.05 0.03 0.05 0.11 0.34 0.36 -0.06 -0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.437 -0.1149 -0.3949 -0.5722 -0.2163 -0.2062 0.859 0.7238 0.8721 0.7255 1.1649 0.8453 2.0406 1.1177 1.391 1.1146 2.3593 1.5452
Actin 0.0776 0.0004 0.0033 0.0003 0.0149 0.0077 0.0372 0.0008 0.015 0.0295 0.0203 0.0141 0.1309 0.0005 0.0071 0.0001 0.0158 0.0008
Bud 0.0016 0.0001 0.0001 0.0001 0.0007 0.0001 0.001 0.0019 0.0014 0.0006 0.0004 0.0005 0.002 0.0003 0.0007 0 0.0004 0.0004
Bud Neck 0.004 0.0001 0.0028 0.0001 0.002 0.0101 0.0022 0.0001 0.0006 0.0001 0.0005 0.0006 0.0016 0.0001 0.0001 0.0001 0.0012 0.0001
Bud Periphery 0.0032 0.0002 0.0002 0.0002 0.0007 0.0004 0.0022 0.0057 0.0033 0.0008 0.0008 0.0012 0.0084 0.0006 0.0033 0 0.0003 0.0009
Bud Site 0.0191 0.0007 0.0017 0.0001 0.0185 0.0002 0.0104 0.0063 0.0098 0.0003 0.0015 0.0004 0.0093 0.0019 0.0048 0 0.0036 0.0001
Cell Periphery 0.0013 0.0003 0.0003 0.0001 0.0004 0.0002 0.0005 0.0005 0.0004 0.0001 0.0001 0.0001 0.0012 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0566 0.0945 0.1824 0.1538 0.2646 0.2767 0.11 0.1236 0.1132 0.0877 0.1173 0.1697 0.037 0.285 0.2019 0.1908 0.0374 0.1993
Cytoplasmic Foci 0.0048 0.0038 0.0097 0.0154 0.0229 0.009 0.0313 0.0059 0.0071 0.0075 0.0168 0.0072 0.0085 0.0083 0.0089 0.001 0.0447 0.0054
Eisosomes 0.0011 0.0003 0.0015 0.0001 0.0004 0.0004 0.0009 0.0003 0.0003 0.0002 0.0003 0.0002 0.0017 0.0008 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0019 0.0008 0.0026 0.0018 0.0025 0.002 0.0075 0.0006 0.0025 0.0016 0.0022 0.0009 0.0215 0.0025 0.0013 0.0007 0.0004 0.0005
Endosome 0.0046 0.0004 0.0032 0.0024 0.0134 0.0011 0.0194 0.0032 0.0033 0.0023 0.0551 0.0051 0.0301 0.0026 0.003 0.0002 0.003 0.0008
Golgi 0.0032 0.0001 0.001 0.0002 0.005 0.0006 0.009 0.0015 0.0021 0.0024 0.0141 0.0088 0.0352 0.0004 0.0018 0 0.0056 0.0006
Lipid Particles 0.0031 0.001 0.0014 0.0009 0.0102 0.0005 0.0147 0.0055 0.0015 0.0074 0.0295 0.0049 0.0061 0.0006 0.0046 0.0001 0.0355 0.0009
Mitochondria 0.001 0.0001 0.0009 0.0003 0.0017 0.0005 0.008 0.0006 0.0009 0.0014 0.0069 0.0063 0.0259 0.0003 0.008 0.0001 0.0312 0.0012
None 0.8084 0.8954 0.7657 0.8196 0.6056 0.6883 0.7061 0.8404 0.8277 0.8292 0.7046 0.7692 0.6229 0.6921 0.7408 0.806 0.7928 0.7772
Nuclear Periphery 0.0016 0.0003 0.001 0.0006 0.0022 0.0004 0.0055 0.0002 0.0012 0.0009 0.0061 0.0004 0.0079 0.0005 0.0005 0.0002 0.0001 0.0001
Nucleolus 0.0005 0.0001 0.0032 0.0002 0.002 0.0001 0.0004 0.0002 0.0003 0.0001 0.0005 0.0001 0.0073 0.0003 0.0004 0 0.0007 0
Nucleus 0.0006 0.0003 0.0042 0.0007 0.0021 0.0004 0.0012 0.0004 0.0008 0.0005 0.0011 0.0003 0.0317 0.0006 0.0005 0.0003 0.0002 0.0001
Peroxisomes 0.0018 0.0001 0.0104 0.0002 0.0081 0.0003 0.0241 0.0015 0.0068 0.0255 0.01 0.0089 0.0034 0.0001 0.0093 0 0.0245 0.011
Punctate Nuclear 0.0025 0.0005 0.0025 0.0018 0.0205 0.0006 0.0045 0.0003 0.0008 0.0003 0.009 0.0005 0.0026 0.0012 0.0016 0.0001 0.0019 0.0003
Vacuole 0.0013 0.0004 0.0017 0.0009 0.0013 0.0004 0.0025 0.0005 0.001 0.0013 0.0016 0.0005 0.0029 0.0009 0.0006 0.0002 0.0002 0.0002
Vacuole Periphery 0.0003 0 0.0002 0.0002 0.0003 0.0001 0.0014 0.0001 0.0002 0.0004 0.0012 0.0002 0.0018 0.0001 0.0002 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0681 5.8365 7.6995 7.8862 7.5246 7.2505 8.5241 8.6298 8.0854 9.7192
Translational Efficiency 0.9306 1.5639 0.8369 0.7626 1.0206 1.5661 0.9578 1.1053 0.8478 0.9607

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
127 209 145 1164 1407 1212 668 107 1534 1421 813 1271

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.18 713.54 948.47 853.56 711.37 738.07 998.54 911.02 710.94 734.46 989.61 858.40
Standard Deviation 65.96 87.81 106.62 115.93 79.35 100.33 100.40 149.43 78.34 98.97 103.33 120.18
Intensity Change Log 2 — 0.014958 0.425566 0.273457 — 0.053158 0.489220 0.356883 — 0.034254 0.457861 0.315925

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000321 0.000766 0.000444 0.000112 0.000844 0.000148 0.001251 0.000108 0.000767 0.000258 0.000512
Bud Neck 0.011175 0.068065 0.002487 0.006823 0.011197 0.042133 0.002404 0.008720 0.011195 0.045948 0.002419 0.006982
Bud Site 0.004234 0.035545 0.018130 0.054043 0.004657 0.026179 0.006273 0.011308 0.004622 0.027557 0.008388 0.050445
Cell Periphery 0.000165 0.000242 0.000292 0.000150 0.000118 0.000195 0.000107 0.000249 0.000122 0.000202 0.000140 0.000158
Cytoplasm 0.557450* 0.286537* 0.343869* 0.550978* 0.561119* 0.352788* 0.543457* 0.294354* 0.560816* 0.343044* 0.507860* 0.529374*
Cytoplasmic Foci 0.237144* 0.335907* 0.033679 0.027938 0.222603* 0.305951* 0.028871 0.068342 0.223807* 0.310357* 0.029729 0.031339
Eisosomes 0.000209 0.000234 0.000111 0.000048 0.000150 0.000270 0.000053 0.000110 0.000155 0.000265 0.000063 0.000053
Endoplasmic Reticulum 0.000275 0.000978 0.007049 0.001469 0.000625 0.000621 0.001826 0.003178 0.000596 0.000673 0.002758 0.001613
Endosome 0.017487 0.058183 0.009511 0.006907 0.013075 0.050911 0.005824 0.064069 0.013440 0.051980 0.006482 0.011720
Golgi 0.000866 0.006397 0.000184 0.003361 0.002526 0.014526 0.000108 0.035236 0.002388 0.013331 0.000122 0.006045
Lipid Particles 0.004213 0.008866 0.003957 0.001007 0.005765 0.007363 0.001607 0.010475 0.005636 0.007584 0.002026 0.001804
Mitochondria 0.000479 0.021093 0.000407 0.001281 0.003183 0.014032 0.000185 0.022780 0.002960 0.015070 0.000224 0.003091
Mitotic Spindle 0.000016 0.001059 0.000974 0.034306 0.003724 0.009248 0.002464 0.040723 0.003417 0.008043 0.002198 0.034846
None 0.001066 0.000741 0.002080 0.004926 0.002743 0.001371 0.002175 0.004335 0.002604 0.001278 0.002158 0.004877
Nuclear Periphery 0.000199 0.000207 0.001757 0.000951 0.000319 0.000789 0.000956 0.002242 0.000309 0.000703 0.001099 0.001060
Nuclear Periphery Foci 0.000271 0.000498 0.006156 0.001222 0.000435 0.000521 0.003468 0.011011 0.000422 0.000518 0.003947 0.002046
Nucleolus 0.000492 0.001093 0.000587 0.000256 0.000723 0.001059 0.000245 0.000321 0.000704 0.001064 0.000306 0.000261
Nucleus 0.074262 0.046619 0.370529* 0.119920 0.100883 0.044174 0.153944* 0.209895* 0.098679 0.044533 0.192572* 0.127495
Peroxisomes 0.001831 0.018951 0.000782 0.000639 0.003427 0.029020 0.000199 0.013486 0.003295 0.027539 0.000303 0.001720
Vacuole 0.087234 0.104848 0.196406 0.181884 0.061626 0.094178 0.245231* 0.187615 0.063746 0.095748 0.236523* 0.182366
Vacuole Periphery 0.000872 0.003617 0.000288 0.001446 0.000988 0.003828 0.000458 0.010299 0.000979 0.003797 0.000428 0.002192

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.52 -4.62 -7.74 -0.10 2.92 -6.01 -2.69 -2.88 0.57 -2.63 -6.30 -4.93 -6.59 3.69 -1.68
Bud Neck -5.68 5.15 3.66 6.40 -3.60 -10.53 8.42 2.61 10.65 -1.51 -11.88 9.15 6.00 14.54 -4.22
Bud Site -4.83 -1.20 -5.83 1.82 -1.43 -10.16 -0.19 -1.41 7.13 -1.30 -11.17 -1.05 -5.97 1.88 -5.00
Cell Periphery -2.05 -4.08 3.09 4.07 7.28 -6.01 3.59 -2.75 0.56 -3.78 -6.60 -0.85 1.16 6.56 1.67
Cytoplasm 10.76 9.89 6.59 -7.16 -6.11 19.89 8.84 10.42 2.40 5.85 22.47 12.01 14.49 -6.14 0.95
Cytoplasmic Foci -4.37 13.70 14.69 18.59 2.97 -9.89 37.58 19.31 25.93 -3.10 -10.94 39.68 40.11 46.11 1.19
Eisosomes -0.77 3.85 7.43 7.40 6.96 -6.48 11.64 2.30 7.42 -5.30 -6.67 11.21 12.50 13.44 2.10
Endoplasmic Reticulum -2.10 -9.25 -13.71 -3.46 6.97 0.03 -11.47 -3.59 -3.59 -1.10 -0.65 -13.09 -11.21 -10.21 4.81
Endosome -5.38 2.49 4.62 8.05 5.53 -13.55 8.44 -1.83 2.44 -2.83 -14.71 8.23 6.45 17.95 0.13
Golgi -4.31 3.44 -0.28 3.96 -1.72 -7.79 5.03 -1.94 -0.09 -2.33 -8.16 5.13 0.26 7.72 -2.98
Lipid Particles -2.76 0.01 4.60 4.93 7.44 -2.09 9.03 0.01 0.50 -1.30 -2.74 8.29 8.22 9.46 1.70
Mitochondria -2.91 0.53 -1.10 2.88 -2.15 -5.30 3.41 -0.87 0.54 -1.28 -5.89 3.39 1.47 6.85 -1.94
Mitotic Spindle -3.91 -2.48 -6.47 -6.04 -6.02 -2.48 0.73 -1.55 -0.95 -1.69 -2.36 0.80 -4.85 -3.06 -5.60
None 0.76 -1.63 -4.74 -6.45 -3.36 1.79 1.31 -0.19 -1.18 -0.78 1.95 1.17 -2.02 -4.11 -4.03
Nuclear Periphery -0.13 -13.10 -20.38 -19.71 1.24 -0.85 -8.22 -2.87 -1.44 -0.95 -0.83 -10.10 -12.10 -2.47 -3.57
Nuclear Periphery Foci -2.06 -7.54 -12.45 -6.46 6.19 -0.43 -12.82 -1.18 -1.16 -0.37 -0.59 -14.49 -3.82 -3.31 5.79
Nucleolus -2.50 -0.26 1.42 4.38 2.08 -2.21 6.71 4.31 5.24 -1.19 -2.65 5.85 5.82 6.71 -0.53
Nucleus 2.86 -11.28 -11.04 -16.25 7.21 15.19 -8.92 -6.42 -8.89 -3.43 15.49 -12.81 -13.78 -23.36 1.82
Peroxisomes -5.94 3.14 4.87 6.50 1.56 -11.63 7.50 -0.84 6.31 -1.96 -12.55 7.57 5.28 14.05 -2.14
Vacuole -1.36 -7.90 -17.35 -16.67 -2.11 -7.68 -24.23 -9.21 -7.85 1.20 -7.99 -25.74 -30.37 -25.16 0.32
Vacuole Periphery -3.86 2.01 1.14 4.70 -1.25 -5.07 3.71 -1.35 -0.25 -1.64 -5.73 4.00 0.51 5.58 -2.03
Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (62%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mkk2

Mkk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available