Standard name
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.99 0.93 0.78 0.74 0.73 0.65 0.58 0.5 0.43 1.0 1.0 1.0 0.97 0.98 0.94 0.85 0.91 0.85 0.92 0.91 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0.11 0.41 0.43 0.42 0.63 0.69 0.7 0.74 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 3
Bud 2 0 3 2 0 5 4 7 5 7 4 11 0 0 0 0 1 0 1 1 3 0 1 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 1 2
Bud Site 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0
Cell Periphery 0 2 4 4 0 2 2 4 4 6 2 7 1 1 1 2 6 2 0 1 0 0 0 0
Cytoplasm 90 317 180 147 192 241 295 367 208 211 119 153 214 339 306 201 119 109 83 297 163 121 193 163
Endoplasmic Reticulum 0 5 3 0 1 0 1 4 0 3 2 3 1 1 0 9 3 7 0 0 1 0 1 2
Endosome 0 0 0 0 3 4 4 9 0 1 2 1 0 0 1 1 3 3 1 3 3 1 0 1
Golgi 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 1 2
Mitochondria 10 12 6 6 23 125 173 212 200 250 167 262 0 0 0 5 1 2 4 8 8 3 4 2
Nucleus 0 0 0 0 1 2 1 5 1 0 3 1 0 0 0 2 1 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 7 4 1 2 1 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 0 0 2 2 5 8 9 6 2 4 8 0 0 0 2 1 0 4 3 1 0 1 10
Unique Cell Count 93 328 187 148 206 308 400 503 318 361 238 354 215 340 307 207 122 116 98 327 193 133 212 201
Labelled Cell Count 103 338 196 161 223 385 492 626 430 482 306 448 216 341 308 222 135 125 98 327 193 133 212 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.4 4.8 4.8 4.4 3.8 3.2 3.9 3.5 3.3 3.5 3.3 5.0 4.9 5.0 5.2 6.1 6.2 4.6 4.7 5.4
Std Deviation (1e-4) 0.9 0.7 1.4 1.8 1.6 1.6 0.8 1.4 1.3 1.1 1.0 1.1 1.2 1.5 1.4 1.2 1.4 1.2 1.4 1.1 1.7
Intensity Change (Log2) -0.02 -0.14 -0.34 -0.6 -0.3 -0.46 -0.54 -0.47 -0.56 0.05 0.03 0.05 0.11 0.34 0.36 -0.06 -0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available