Standard name | |
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Description | Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 1 | 4 | 0 | 0 | 1 | – | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 6 | – | 1 | 36 | 28 | 9 | 14 | 18 | 20 |
Bud | 4 | 1 | 1 | 1 | 1 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | – | 0 | 4 | 1 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 2 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 1 | 1 | 3 | 2 | – | 1 | 5 | 7 | 7 | 5 | 1 | 1 | 2 | 9 | – | 3 | 1 | 1 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 2 | 0 | 1 | 1 | 5 | – | 2 | 3 | 1 | 0 | 2 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 5 | 0 | 1 | 0 | 1 | 1 |
Golgi | 68 | 34 | 1 | 0 | 0 | 1 | – | 2 | 1 | 0 | 3 | 1 | 3 | 7 | 7 | 35 | – | 5 | 137 | 88 | 26 | 47 | 41 | 49 |
Mitochondria | 713 | 276 | 105 | 21 | 44 | 90 | – | 178 | 165 | 167 | 122 | 142 | 223 | 128 | 169 | 7 | – | 5 | 680 | 228 | 71 | 95 | 75 | 110 |
Nucleus | 4 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 1 | 1 | 0 | 0 | 0 | – | 1 | 2 | 1 | 3 | 6 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 90 | 41 | 16 | 3 | 18 | 22 | – | 39 | 40 | 31 | 21 | 30 | 29 | 25 | 10 | 16 | – | 18 | 20 | 11 | 3 | 4 | 11 | 9 |
Peroxisomes | 4 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 7 | 5 | 1 | 5 | 6 | 3 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 89 | 61 | 3 | 0 | 3 | 5 | – | 8 | 2 | 4 | 0 | 1 | 18 | 18 | 15 | 24 | – | 7 | 30 | 8 | 1 | 3 | 4 | 6 |
Unique Cell Count | 830 | 325 | 108 | 22 | 48 | 97 | 192 | 176 | 180 | 138 | 155 | 230 | 147 | 178 | 87 | 35 | 944 | 385 | 120 | 180 | 163 | 209 | ||
Labelled Cell Count | 1012 | 419 | 132 | 26 | 69 | 124 | 231 | 217 | 213 | 164 | 189 | 276 | 182 | 207 | 104 | 43 | 944 | 385 | 120 | 180 | 163 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 43.9 | 90.8 | 39.4 | 54.6 | 60.8 | 42.7 | – | 35.0 | 33.0 | 29.9 | 30.2 | 32.2 | 63.7 | 67.9 | 66.7 | 68.7 | – | 68.6 | 73.6 | 75.4 | 78.8 |
Std Deviation (1e-4) | 14.9 | 29.6 | 14.9 | 23.5 | 25.9 | 20.0 | – | 14.2 | 14.8 | 13.4 | 14.2 | 16.7 | 19.7 | 25.1 | 25.0 | 34.2 | – | 35.2 | 28.4 | 32.4 | 34.6 |
Intensity Change (Log2) | – | – | – | 0.47 | 0.62 | 0.11 | – | -0.17 | -0.26 | -0.4 | -0.38 | -0.29 | 0.69 | 0.78 | 0.76 | 0.8 | – | 0.8 | 0.9 | 0.94 | 1.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.0 | – | 0 |
Mitochondria | -0.4 | -1.5 | -1.5 | – | -1.6 | -1.3 | -1.6 | -2.6 | -1.9 | -0.1 | -2.9 | -0.9 | -12.5 | – | -10.1 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 3.2 | 1.4 | – | 1.2 | 1.6 | 0.5 | 0.1 | 1.0 | -0.6 | 0.5 | -2.6 | 0.7 | – | 4.4 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.7 | 1.9 | 5.0 | – | 0 |