Standard name
Description Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 – 0 0 0.07 0.08 0.08 0.11 0.1
Bud 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 – 0 0 0 0.05 0 0 0 0 0.1 – 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0.08 0.1 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.4 – 0.14 0.15 0.23 0.22 0.27 0.26 0.24
Mitochondria 0.86 0.85 0.97 0.95 0.92 0.93 – 0.93 0.94 0.93 0.88 0.92 0.97 0.87 0.95 0.08 – 0.14 0.72 0.59 0.6 0.53 0.46 0.53
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.11 0.13 0.15 0.14 0.38 0.23 – 0.2 0.23 0.17 0.15 0.19 0.13 0.17 0.06 0.18 – 0.51 0 0 0 0 0.07 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.19 0 0 0.06 0.05 – 0 0 0 0 0 0.08 0.12 0.08 0.28 – 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 1 4 0 0 1 – 0 0 2 1 0 0 1 3 6 – 1 36 28 9 14 18 20
Bud 4 1 1 1 1 0 – 1 0 0 1 1 0 1 0 0 – 0 4 1 1 1 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 3 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 10 2 0 0 0 1 – 0 0 0 0 0 2 0 0 1 – 0 0 0 0 0 0 0
Cytoplasm 4 2 1 1 3 2 – 1 5 7 7 5 1 1 2 9 – 3 1 1 0 1 0 0
Endoplasmic Reticulum 1 0 0 0 0 0 – 0 0 1 5 2 0 1 1 5 – 2 3 1 0 2 0 1
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 1 5 0 1 0 1 1
Golgi 68 34 1 0 0 1 – 2 1 0 3 1 3 7 7 35 – 5 137 88 26 47 41 49
Mitochondria 713 276 105 21 44 90 – 178 165 167 122 142 223 128 169 7 – 5 680 228 71 95 75 110
Nucleus 4 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0
Nuclear Periphery 3 1 1 0 0 0 – 1 2 1 3 6 0 0 0 0 – 0 3 1 0 0 0 0
Nucleolus 90 41 16 3 18 22 – 39 40 31 21 30 29 25 10 16 – 18 20 11 3 4 11 9
Peroxisomes 4 0 0 0 0 1 – 1 1 0 0 0 0 0 0 1 – 1 7 5 1 5 6 3
SpindlePole 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 – 0 2 0 0 0 0 1
Vac/Vac Membrane 89 61 3 0 3 5 – 8 2 4 0 1 18 18 15 24 – 7 30 8 1 3 4 6
Unique Cell Count 830 325 108 22 48 97 192 176 180 138 155 230 147 178 87 35 944 385 120 180 163 209
Labelled Cell Count 1012 419 132 26 69 124 231 217 213 164 189 276 182 207 104 43 944 385 120 180 163 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.9 90.8 39.4 54.6 60.8 42.7 – 35.0 33.0 29.9 30.2 32.2 63.7 67.9 66.7 68.7 – 68.6 73.6 75.4 78.8
Std Deviation (1e-4) 14.9 29.6 14.9 23.5 25.9 20.0 – 14.2 14.8 13.4 14.2 16.7 19.7 25.1 25.0 34.2 – 35.2 28.4 32.4 34.6
Intensity Change (Log2) – – – 0.47 0.62 0.11 – -0.17 -0.26 -0.4 -0.38 -0.29 0.69 0.78 0.76 0.8 – 0.8 0.9 0.94 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 7.0 – 0
Mitochondria -0.4 -1.5 -1.5 – -1.6 -1.3 -1.6 -2.6 -1.9 -0.1 -2.9 -0.9 -12.5 – -10.1
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 3.2 1.4 – 1.2 1.6 0.5 0.1 1.0 -0.6 0.5 -2.6 0.7 – 4.4
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 – 0 0 0 0 0 1.8 2.7 1.9 5.0 – 0