Standard name
Human Ortholog
Description Inner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 – 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.06 0.05 0 – 0.12 0 0 0.06 0.07 0.06 0.08 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 – 0 0.09 0.07 0 0 0 0 0 0.07 0.13 0.19 0.12 0.1 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 – 0 0.06 0.05 0 0 0.06 0 0 0.08 0.1 0.15 0.13 0.08 0.15 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 – 0.06 0.09 0 0 0.17 0.14 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0.06 0.12 0 0.06 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0.13 0.16 – 0 0.24 0.3 0.33 0.26 0.28 0.29 0.21 0.14 0.14 0.17 0.12 0.12 0.1 0 0 0 0 0 0
Peroxisomes 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.16 0 0.06 0 0 0 0 0 0
SpindlePole 0.84 0.89 0.95 – 0.94 0.79 0.8 0.88 0.8 0.81 0.76 0.79 0.84 0.82 0.72 0.8 0.83 0.73 0.88 0.85 0.86 0.83 0.8 0.78
Vac/Vac Membrane 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 1 0 1 1 1 1 0 0 0 0 2 3 0 0 2 1
Bud 4 13 1 – 0 0 3 1 5 2 7 5 0 0 2 3 2 4 4 6 0 1 7 9
Bud Neck 2 10 0 – 0 0 6 1 0 0 1 1 2 1 1 4 4 3 1 5 0 1 2 7
Bud Site 12 30 0 – 2 1 4 5 7 2 13 8 6 2 2 7 1 3 – – – – – –
Cell Periphery 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 15 1 – 0 3 7 3 3 1 6 2 15 28 51 28 20 33 2 4 1 2 6 7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 3 1 0 0 0 1 2 0
Endosome 14 16 0 – 0 2 5 2 0 2 3 3 16 21 40 32 16 27 5 26 0 4 7 5
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 2 1
Mitochondria 7 30 0 – 1 3 1 1 17 5 15 14 0 1 2 7 6 5 2 6 0 1 5 3
Nucleus 0 11 0 – 1 4 4 5 6 1 8 8 4 1 3 1 3 3 0 3 0 2 0 0
Nuclear Periphery 0 5 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 2
Nucleolus 48 78 3 – 0 8 31 29 26 10 47 27 28 30 45 28 26 19 0 15 0 1 4 2
Peroxisomes 11 17 0 – 0 0 1 1 4 1 3 0 5 4 15 38 8 12 1 5 0 2 4 3
SpindlePole 177 519 18 – 16 26 83 78 80 29 124 101 173 171 194 195 174 135 191 522 46 115 215 176
Vac/Vac Membrane 3 6 0 – 0 0 2 1 0 0 0 0 1 1 1 1 5 1 1 4 0 0 2 0
Unique Cell Count 210 586 19 17 33 104 89 100 36 164 128 206 209 269 243 209 186 219 612 54 138 269 227
Labelled Cell Count 295 750 23 20 47 147 127 149 53 228 171 251 261 357 345 268 248 219 612 54 138 269 227


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.4 4.8 – 4.7 5.5 5.5 5.1 4.6 4.6 4.7 4.7 5.9 6.1 5.5 4.9 6.6 6.2 6.0 6.1 6.2
Std Deviation (1e-4) 1.3 0.8 1.1 – 0.9 1.0 1.5 1.2 1.2 0.7 1.3 0.9 1.1 1.3 1.3 1.9 2.3 1.7 1.3 1.4 1.6
Intensity Change (Log2) – – – – -0.04 0.18 0.19 0.08 -0.08 -0.06 -0.03 -0.05 0.28 0.33 0.2 0.02 0.45 0.36 0.31 0.33 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.2758 0.0071 0.0001 0 0 0 0.0326 0.0002 0.0007 0.004 0.0115 0.0004 – – – – – –
Bud 0.0047 0.0005 0.0002 0 0 0 0.0016 0.0004 0.0003 0.0006 0.0005 0.0002 – – – – – –
Bud Neck 0.0253 0.0059 0.0096 0.0037 0 0.0001 0.1377 0.001 0.0014 0.0004 0.0298 0.0006 – – – – – –
Bud Periphery 0.0061 0.0006 0.0003 0 0 0 0.0029 0.0007 0.0007 0.0046 0.0011 0.0006 – – – – – –
Bud Site 0.049 0.0079 0.0004 0.0001 0 0 0.0616 0.0041 0.0067 0.0005 0.0028 0.0006 – – – – – –
Cell Periphery 0.0026 0.0001 0 0 0 0 0.0188 0.0001 0.0001 0.0001 0.0003 0.0001 – – – – – –
Cytoplasm 0.0077 0.0022 0.0002 0.0001 0 0 0.0556 0.0011 0.0028 0.0003 0.0011 0.0006 – – – – – –
Cytoplasmic Foci 0.1159 0.0129 0.0061 0.025 0.0046 0.0023 0.3144 0.0183 0.0204 0.0116 0.0741 0.0109 – – – – – –
Eisosomes 0.0032 0 0 0 0 0 0.0171 0 0 0 0.0002 0 – – – – – –
Endoplasmic Reticulum 0.0287 0.0003 0 0 0 0 0.0312 0.0002 0.0002 0.0003 0.0006 0.0005 – – – – – –
Endosome 0.0381 0.002 0.0006 0.0007 0 0 0.0208 0.0046 0.0118 0.0055 0.0327 0.0055 – – – – – –
Golgi 0.0251 0.0006 0.0004 0.0008 0 0.0001 0.0276 0.0021 0.004 0.0098 0.0208 0.0014 – – – – – –
Lipid Particles 0.0831 0.0047 0.0045 0.0118 0.0014 0.0015 0.0496 0.0101 0.0208 0.0245 0.231 0.0126 – – – – – –
Mitochondria 0.0653 0.0002 0.0001 0 0 0 0.0062 0.0006 0.0101 0.0174 0.0067 0.0011 – – – – – –
None 0.0065 0.0027 0.0002 0.0001 0 0 0.1103 0.0106 0.0016 0.0003 0.0011 0.0001 – – – – – –
Nuclear Periphery 0.0374 0.0078 0.0047 0.0014 0.0001 0.0002 0.0038 0.0057 0.0034 0.0061 0.0029 0.0013 – – – – – –
Nucleolus 0.0051 0.0779 0.0499 0.0043 0.0039 0.0009 0.0057 0.0547 0.0324 0.0023 0.0081 0.0067 – – – – – –
Nucleus 0.0139 0.036 0.0167 0.0008 0.0002 0.0003 0.0038 0.028 0.0085 0.0012 0.0018 0.0011 – – – – – –
Peroxisomes 0.1844 0.0012 0.0018 0.0022 0 0.0001 0.0436 0.0092 0.0078 0.0037 0.0706 0.0019 – – – – – –
Punctate Nuclear 0.0145 0.8268 0.904 0.949 0.9897 0.9945 0.0495 0.8471 0.8646 0.9048 0.4979 0.9524 – – – – – –
Vacuole 0.0052 0.0019 0.0002 0 0 0 0.0047 0.0009 0.0013 0.0006 0.0028 0.0011 – – – – – –
Vacuole Periphery 0.0026 0.0007 0.0001 0 0 0 0.0007 0.0004 0.0004 0.0012 0.0015 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4804 6.4723 4.8027 5.1387 16.897 7.2362 3.5993 4.8585 5.0722 13.0874
Translational Efficiency 2.4801 2.0181 0.4931 1.6382 1.3218 5.5045 2.6238 1.3828 1.6484 2.0177

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
13 10 1811 1583 1738 1593 339 9 1751 1603 2150 1592

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 518.05 324.82 815.21 870.94 626.02 816.35 854.22 386.90 625.22 813.28 821.36 868.20
Standard Deviation 185.44 5.35 113.72 116.96 87.18 119.95 109.28 13.50 88.80 125.68 113.92 122.15
Intensity Change Log 2 — -0.673451 0.654080 0.749482 — 0.382979 0.448399 -0.694248 — -0.003662 0.545180 0.136773

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000243 0.000027 0.000417 0.000290 0.000204 0.000342 0.000259 0.000062 0.000205 0.000340 0.000392 0.000289
Bud Neck 0.001082 0.000012 0.003325 0.002810 0.003483 0.002764 0.003281 0.040963 0.003466 0.002746 0.003318 0.003025
Bud Site 0.000788 0.000028 0.008806 0.012162 0.004231 0.003947 0.007563 0.008758 0.004206 0.003922 0.008610 0.012143
Cell Periphery 0.000089 0.000050 0.000077 0.000040 0.000212 0.000174 0.000160 0.010353 0.000211 0.000173 0.000090 0.000099
Cytoplasm 0.000924 0.000047 0.008175 0.006536 0.028355 0.003565 0.090228 0.000749 0.028151 0.003543 0.021113 0.006503
Cytoplasmic Foci 0.048823 0.000015 0.029143 0.033534 0.192912* 0.018287 0.029905 0.000281 0.191842* 0.018173 0.029263 0.033346
Eisosomes 0.000102 0.000035 0.000025 0.000021 0.000089 0.000124 0.000053 0.001065 0.000089 0.000123 0.000029 0.000027
Endoplasmic Reticulum 0.000536 0.000001 0.000453 0.000221 0.000789 0.001408 0.001082 0.000002 0.000787 0.001399 0.000552 0.000220
Endosome 0.002049 0.000002 0.005973 0.005244 0.006965 0.004027 0.004341 0.000115 0.006928 0.004001 0.005716 0.005215
Golgi 0.018920 0.000005 0.003348 0.005755 0.010951 0.003418 0.000753 0.000006 0.011010 0.003397 0.002939 0.005723
Lipid Particles 0.022757 0.000018 0.003418 0.001867 0.043874 0.014745 0.002141 0.000061 0.043717 0.014653 0.003217 0.001856
Mitochondria 0.001439 0.000011 0.000664 0.001417 0.001745 0.001748 0.000248 0.000031 0.001743 0.001737 0.000598 0.001409
Mitotic Spindle 0.042760 0.000270 0.892433* 0.894740* 0.505134* 0.711341* 0.680348* 0.000573 0.501701* 0.706905* 0.858992* 0.889685*
None 0.760542* 0.999280* 0.001249 0.000250 0.014851 0.004376 0.018800 0.928792* 0.020387 0.010582 0.004017 0.005499
Nuclear Periphery 0.000076 0.000000 0.000387 0.000245 0.000488 0.000997 0.000713 0.000005 0.000485 0.000991 0.000439 0.000244
Nuclear Periphery Foci 0.035906 0.000059 0.014724 0.008948 0.091920 0.128034 0.022785 0.006450 0.091505 0.127236 0.015995 0.008934
Nucleolus 0.036272 0.000057 0.007254 0.003662 0.034170 0.061196 0.009834 0.000081 0.034185 0.060814 0.007661 0.003641
Nucleus 0.005228 0.000015 0.003795 0.002041 0.015196 0.021274 0.097929 0.000016 0.015121 0.021141 0.018638 0.002029
Peroxisomes 0.021265 0.000063 0.012235 0.013775 0.043604 0.017215 0.005033 0.001268 0.043438 0.017108 0.011099 0.013704
Vacuole 0.000153 0.000002 0.003266 0.004944 0.000726 0.000892 0.024408 0.000368 0.000722 0.000887 0.006600 0.004919
Vacuole Periphery 0.000045 0.000000 0.000833 0.001495 0.000100 0.000129 0.000136 0.000002 0.000100 0.000128 0.000723 0.001487

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.94 -4.29 -2.50 0.31 1.74
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.01 -6.71 -4.84 -3.34 1.10
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.84 -11.27 -9.15 -8.06 -0.63
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.10 4.76 0.99 1.00 -0.25
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 5.80 -1.23 1.32 -3.97 2.52
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 24.04 21.13 19.72 -5.99 -1.17
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.31 18.21 16.56 13.54 1.51
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -6.64 -0.51 8.07 11.74 4.52
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 7.53 2.52 3.95 -1.80 1.52
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 12.06 12.39 8.98 0.36 -0.77
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 9.70 15.69 16.25 7.75 2.21
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.83 1.73 -0.15 0.61 -0.90
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -14.65 -31.02 -32.56 -16.22 -2.80
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.04 6.00 3.10 1.83 -1.88
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -11.22 -1.86 3.13 11.37 3.84
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.54 20.36 22.70 21.73 3.95
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.59 8.24 10.46 13.95 3.04
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.10 -3.86 1.52 6.37 5.29
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 9.97 12.70 11.08 1.43 -1.08
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.66 -7.47 -4.84 -4.51 1.35
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.46 -2.71 -2.37 -2.18 -0.85
Standard name
Human Ortholog
Description Inner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
Localization
Cell Percentages spindle pole (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc29

Spc29


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc29-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available