TheCellVision

HOS3

Standard name	HOS3 ORF YPL116W Aliases histone deacetylase
Human Ortholog
Description	Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats

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Scale images

HOS3

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0.05	0	0.05	0	0	0	0	–	0	0	0	0	0	0.05	0.11	0	0	0	0	0	0	0
Bud	0	0	0	0	0.05	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0.1	0.07	0.06	0.05	0	0
Bud Site	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0.07	0.11	0.08	0	0	0	0	0	0
Cytoplasm	0.93	0.88	0.91	0.91	0.85	0.79	0.77	0.74	0.69	–	0.59	0.7	0.96	0.94	0.88	0.8	0.78	0.92	0.79	0.74	0.76	0.7	0.8	0.79
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0.06	0	0.05	0	0	0	0.08	0.1	0.07
Endosome	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0.08	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0.05	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0.05	0.16	0.17	0.13	0.37	–	0.39	0.24	0	0	0	0.05	0.07	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0.05	0.09	0	–	0.08	0.07	0	0	0	0.08	0	0.08	0	0	0	0.07	0.05	0.05

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	3	9	24	14	26	12	17	9	8	–	3	10	3	7	12	4	5	1	7	11	5	1	3	2
Bud	5	15	11	11	23	25	28	8	22	–	20	26	8	3	12	2	2	0	6	19	17	3	1	7
Bud Neck	1	0	1	4	3	2	2	1	0	–	1	0	0	0	0	0	0	0	31	29	31	7	1	3
Bud Site	0	0	3	0	0	1	2	1	1	–	0	3	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	2	4	19	13	8	14	13	8	9	–	15	14	2	12	6	6	5	5	0	0	0	0	0	0
Cytoplasm	284	357	439	404	408	490	660	475	393	–	325	425	333	419	467	69	36	59	252	310	401	118	271	349
Endoplasmic Reticulum	5	9	5	3	7	12	21	4	9	–	4	5	7	10	6	5	2	3	4	5	4	13	33	30
Endosome	3	16	1	6	5	15	10	13	4	–	2	7	0	7	41	0	0	1	9	10	12	5	0	2
Golgi	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	4	1	0	1	2	3	0	0	2
Mitochondria	10	9	8	9	22	99	143	84	209	–	216	144	8	1	2	4	3	0	0	1	19	3	9	13
Nucleus	0	3	1	0	4	2	11	8	8	–	5	13	1	1	0	0	0	1	0	1	1	0	0	0
Nuclear Periphery	2	0	0	1	1	2	9	0	1	–	1	2	0	0	0	0	0	0	2	1	2	1	0	0
Nucleolus	4	3	0	0	1	4	6	8	3	–	5	5	0	0	1	0	0	0	0	1	0	0	0	0
Peroxisomes	0	2	5	0	2	5	3	2	1	–	1	1	0	1	8	0	0	1	0	1	3	0	1	0
SpindlePole	0	0	6	2	6	8	3	18	3	–	5	9	0	1	2	1	0	0	1	5	10	0	0	1
Vac/Vac Membrane	4	11	2	10	8	20	46	60	25	–	45	42	0	4	2	7	1	5	1	15	7	11	15	22
Unique Cell Count	304	407	482	445	481	623	853	641	568		554	604	346	448	530	86	46	64	321	420	526	169	341	443
Labelled Cell Count	323	438	525	477	524	711	974	699	696		648	706	362	466	559	102	55	76	321	420	526	169	341	443

Yeast GFP Assignment

Bud Neck

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	6.7	6.8	6.4	5.9	6.5	5.7	5.8	6.2	5.3	–	4.9	5.8	6.1	6.8	7.0	13.6	13.1	13.0	6.0	6.3	6.7
Std Deviation (1e^-4)	0.8	1.2	0.8	0.9	1.1	0.9	0.8	0.9	1.0	–	0.8	1.0	0.9	0.8	1.0	2.1	1.8	2.7	0.8	1.0	1.2
Intensity Change (Log₂)	–	–	–	-0.13	0.01	-0.18	-0.15	-0.06	-0.28	–	-0.41	-0.16	-0.07	0.07	0.12	1.08	1.02	1.01	-0.11	-0.04	0.04

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	-1.4	0.3	-2.8	-3.0	-3.5	-3.4	–	0	-3.1	-3.3	-2.9	-2.3	0	0	0
Bud	0.2	2.1	1.6	1.0	-1.3	1.5	–	0	1.8	0	-2.0	0	0	0	0
Bud Neck	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cell Periphery	-0.9	-2.1	-1.6	-2.8	-2.9	-2.4	–	0	-1.5	-3.0	-1.1	-2.9	0	0	0
Cytoplasm	-0.2	-3.0	-5.6	-6.3	-7.2	-8.7	–	-11.8	-8.4	2.9	1.4	-1.5	-3.0	-2.8	0.3
Endoplasmic Reticulum	0	0.6	1.2	1.8	0	0.8	–	0	0	1.2	1.4	0.1	0	0	0
Endosome	0	0	3.0	0	2.7	0	–	0	0	0	0	6.0	0	0	0
Golgi	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Mitochondria	0.4	2.6	7.9	8.4	6.9	14.0	–	0	10.5	0.7	0	0	0	0	0
Nucleus	0	0	0	0	0	0	–	0	2.8	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	–	0	0	0	0	0.7	0	0	0
SpindlePole	0	0	0.1	0	1.8	0	–	0	0.3	0	0	0	0	0	0
Vacuole	2.5	0	3.3	4.7	6.5	4.1	–	5.9	5.4	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	1.1079	1.6477	1.4266	1.6763	1.3909	1.6134	1.7846	2.5472	2.4757	2.3875	2.3438	2.457	1.4927	2.3709	2.1946	2.3628	2.5399	2.1542
Actin	0.0154	0.0038	0.0042	0.0011	0.0609	0.007	0.0281	0.006	0.0268	0.0234	0.1093	0.0134	0.0331	0.0008	0.0157	0.0057	0.0481	0.013
Bud	0.0049	0.0066	0.002	0.0006	0.0034	0.0025	0.0017	0.0055	0.0021	0.0013	0.0042	0.0017	0.0016	0.0019	0.0027	0.0009	0.001	0.0009
Bud Neck	0.0111	0.0067	0.1246	0.6848	0.5924	0.1145	0.0088	0.0042	0.1263	0.5063	0.5428	0.1324	0.011	0.0064	0.1317	0.6057	0.7523	0.1259
Bud Periphery	0.0053	0.0053	0.0017	0.0006	0.0066	0.0032	0.0016	0.0053	0.0024	0.0013	0.0116	0.0042	0.0013	0.0007	0.0024	0.001	0.0018	0.0016
Bud Site	0.0437	0.1186	0.041	0.0034	0.0097	0.002	0.0305	0.055	0.0206	0.0051	0.0119	0.0042	0.066	0.0702	0.028	0.0045	0.0023	0.0012
Cell Periphery	0.0025	0.0013	0.001	0.0003	0.0011	0.0004	0.0008	0.0006	0.0009	0.0004	0.0011	0.0004	0.0009	0.0004	0.0009	0.0006	0.0003	0.0002
Cytoplasm	0.5699	0.6213	0.619	0.2175	0.1044	0.6696	0.6324	0.771	0.6403	0.3195	0.0051	0.7267	0.6246	0.828	0.7032	0.3238	0.0139	0.7392
Cytoplasmic Foci	0.0635	0.0406	0.0461	0.0389	0.0407	0.0385	0.0737	0.0468	0.0566	0.0596	0.038	0.0237	0.0729	0.0101	0.0346	0.0228	0.0194	0.0216
Eisosomes	0.0004	0.0001	0.0004	0	0.0002	0.0001	0.0003	0.0001	0.0002	0.0001	0.0005	0.0001	0.0004	0	0.0001	0	0.0001	0
Endoplasmic Reticulum	0.0157	0.0072	0.0054	0.0014	0.0074	0.0148	0.0211	0.0068	0.0106	0.0013	0.0022	0.0094	0.0216	0.0048	0.0058	0.002	0.0028	0.0169
Endosome	0.0509	0.0381	0.0224	0.0197	0.0235	0.0453	0.0549	0.0351	0.04	0.041	0.0632	0.0363	0.0364	0.01	0.0227	0.0086	0.0258	0.0282
Golgi	0.0064	0.0043	0.0044	0.0018	0.0111	0.0044	0.0194	0.004	0.01	0.0102	0.0658	0.0081	0.0113	0.0011	0.0054	0.0018	0.0079	0.0041
Lipid Particles	0.0192	0.0036	0.0007	0.0004	0.0079	0.0022	0.0209	0.0013	0.0072	0.0016	0.03	0.0024	0.0133	0.0002	0.0019	0.0003	0.0084	0.0047
Mitochondria	0.0047	0.0016	0.0105	0.0005	0.0011	0.0015	0.0172	0.0023	0.0055	0.0022	0.0423	0.0028	0.0267	0.0003	0.0052	0.0002	0.0041	0.0021
None	0.1092	0.1	0.105	0.0191	0.0249	0.0463	0.0401	0.0271	0.0201	0.0016	0.0025	0.0097	0.041	0.0474	0.0276	0.0108	0.0013	0.0208
Nuclear Periphery	0.03	0.0054	0.0008	0.0007	0.0574	0.0187	0.0086	0.0027	0.0031	0.0006	0.0283	0.0084	0.0049	0.001	0.0016	0.0004	0.0155	0.0047
Nucleolus	0.0012	0.0022	0.0005	0.0004	0.0038	0.0011	0.0012	0.0007	0.0008	0.0001	0.0018	0.0004	0.0008	0.0016	0.0003	0.0024	0.0048	0.0011
Nucleus	0.0156	0.0175	0.0021	0.0029	0.0061	0.0105	0.0032	0.0028	0.002	0.0004	0.0022	0.0025	0.0052	0.0056	0.0013	0.0029	0.006	0.0035
Peroxisomes	0.0051	0.0016	0.0012	0.0005	0.0044	0.0021	0.0169	0.0075	0.0102	0.0206	0.021	0.0035	0.0131	0.0001	0.0015	0.0002	0.0023	0.0017
Punctate Nuclear	0.0067	0.004	0.0013	0.0011	0.025	0.0045	0.0089	0.0023	0.0042	0.001	0.0095	0.0048	0.0074	0.0008	0.0022	0.0008	0.0782	0.0036
Vacuole	0.0144	0.0091	0.0049	0.0039	0.0064	0.0093	0.008	0.0113	0.0086	0.0017	0.0041	0.0038	0.0051	0.0079	0.0042	0.0041	0.0022	0.0037
Vacuole Periphery	0.0042	0.0011	0.0006	0.0004	0.0017	0.0017	0.0018	0.0016	0.0015	0.0006	0.0026	0.0012	0.0012	0.0006	0.001	0.0006	0.0015	0.0011

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	19.4966	44.7496	9.088	12.8293	17.7631	17.3793	29.8864	28.288	19.0449	23.168
Translational Efficiency	0.8208	0.6034	0.7856	0.5039	0.7783	0.8076	0.7698	0.5623	0.6368	0.5799

Hit Data

Dataset	Hit
Protein Concentration	✔
Protein Localization	✔
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1528	1300	734	1270	1965	1102	1263	174	3493	2402	1997	1444

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	735.14	861.53	1187.27	1029.42	842.81	854.69	1063.74	1090.74	795.71	858.39	1109.14	1036.81
Standard Deviation	91.36	126.66	161.58	134.76	98.80	121.91	130.01	134.17	109.52	124.55	154.38	136.16
Intensity Change Log ₂	—	0.228882	0.691557	0.485741	—	0.020194	0.335866	0.372028	—	0.121183	0.512521	0.426122

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000414	0.000947	0.001616	0.002780	0.000483	0.000682	0.000891	0.003021	0.000453	0.000826	0.001158	0.002809
Bud Neck	0.048696	0.031225	0.044733	0.048907	0.057344	0.045970	0.085673	0.058732	0.053561	0.037990	0.070626	0.050091
Bud Site	0.050009	0.073493	0.201354*	0.214330*	0.072069	0.076012	0.243437*	0.265094*	0.062419	0.074649	0.227969*	0.220447*
Cell Periphery	0.000260	0.000179	0.000214	0.000172	0.000259	0.000229	0.000187	0.000170	0.000259	0.000202	0.000197	0.000171
Cytoplasm	0.470186*	0.476838*	0.524774*	0.369277*	0.480496*	0.436412*	0.367019*	0.236773*	0.475986*	0.458291*	0.425002*	0.353310*
Cytoplasmic Foci	0.234506*	0.233713*	0.059985	0.050930	0.218520*	0.221984*	0.049439	0.077004	0.225513*	0.228332*	0.053315	0.054072
Eisosomes	0.000148	0.000086	0.000024	0.000031	0.000129	0.000137	0.000045	0.000041	0.000137	0.000110	0.000037	0.000032
Endoplasmic Reticulum	0.004362	0.002521	0.001159	0.001567	0.005569	0.001628	0.001783	0.003949	0.005041	0.002111	0.001553	0.001854
Endosome	0.044246	0.045729	0.009002	0.021000	0.031792	0.056703	0.022081	0.037238	0.037240	0.050763	0.017273	0.022956
Golgi	0.010667	0.012027	0.000939	0.002718	0.011220	0.015270	0.000672	0.001339	0.010978	0.013514	0.000770	0.002552
Lipid Particles	0.012317	0.004308	0.001657	0.003753	0.007934	0.007250	0.002027	0.004573	0.009851	0.005658	0.001891	0.003852
Mitochondria	0.004537	0.002812	0.000250	0.002401	0.003735	0.006939	0.000581	0.001358	0.004086	0.004705	0.000460	0.002275
Mitotic Spindle	0.009245	0.015227	0.028388	0.153702*	0.010999	0.015124	0.036492	0.111581*	0.010232	0.015179	0.033513	0.148627*
None	0.003327	0.000549	0.001181	0.000479	0.001223	0.000405	0.000669	0.000349	0.002143	0.000483	0.000858	0.000463
Nuclear Periphery	0.000367	0.000212	0.001507	0.004229	0.000402	0.000239	0.001635	0.003702	0.000386	0.000224	0.001588	0.004165
Nuclear Periphery Foci	0.002069	0.002993	0.008996	0.022526	0.003797	0.001784	0.006960	0.023722	0.003041	0.002438	0.007708	0.022670
Nucleolus	0.000893	0.000617	0.003435	0.000215	0.000834	0.000849	0.001295	0.000829	0.000860	0.000723	0.002082	0.000289
Nucleus	0.015019	0.006624	0.037442	0.005323	0.013898	0.008396	0.018927	0.006451	0.014388	0.007437	0.025733	0.005459
Peroxisomes	0.008351	0.009992	0.009329	0.017959	0.008487	0.012855	0.013360	0.026891	0.008427	0.011305	0.011879	0.019035
Vacuole	0.075000	0.076323	0.063440	0.075157	0.067355	0.086179	0.146214	0.134129	0.070699	0.080845	0.115790	0.082263
Vacuole Periphery	0.005381	0.003585	0.000574	0.002545	0.003455	0.004954	0.000611	0.003054	0.004298	0.004213	0.000598	0.002607

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-3.06	-5.19	-5.45	-3.59	-1.46	-2.31	-4.15	-1.49	-1.31	-1.18	-3.70	-6.54	-5.57	-4.28	-3.34
Bud Neck	3.98	2.85	3.42	-0.94	0.21	2.26	-1.28	2.75	1.03	3.63	4.68	0.10	5.75	0.95	5.52
Bud Site	-4.70	-10.04	-14.06	-10.37	-0.36	-0.65	-13.14	-5.30	-5.08	0.16	-3.18	-16.84	-13.84	-11.76	1.49
Cell Periphery	4.42	0.87	7.83	2.37	6.66	1.13	4.35	5.23	4.27	1.89	3.43	4.58	7.90	4.46	4.87
Cytoplasm	-0.64	-3.32	3.34	3.74	5.62	3.81	7.76	8.57	6.39	4.38	2.17	4.22	6.17	4.25	2.15
Cytoplasmic Foci	0.19	30.55	33.10	29.93	1.15	-0.47	35.21	16.39	15.32	-3.68	-0.49	46.95	42.42	37.20	-1.69
Eisosomes	5.47	14.63	14.18	6.96	-0.66	-0.58	10.96	10.42	8.19	0.73	3.19	17.51	18.02	11.27	2.74
Endoplasmic Reticulum	1.97	4.18	4.68	1.86	1.09	5.02	4.90	1.94	-0.91	-0.86	4.62	6.34	6.58	1.60	0.81
Endosome	-0.43	17.46	12.69	11.24	-4.59	-8.27	7.43	0.77	4.90	-1.83	-6.34	15.93	11.96	15.40	-1.57
Golgi	-1.29	16.42	9.62	9.89	-1.38	-2.96	17.60	15.71	11.55	-1.45	-3.03	24.02	12.30	12.68	-1.69
Lipid Particles	5.65	7.32	6.41	1.40	-1.82	0.46	7.41	3.14	2.21	-1.22	4.38	10.34	7.78	3.05	-2.73
Mitochondria	2.18	8.54	4.52	1.53	-3.59	-2.01	6.00	4.44	3.94	-1.08	-0.69	9.80	4.95	3.71	-3.31
Mitotic Spindle	-2.24	-4.89	-14.80	-13.60	-11.13	-1.43	-6.67	-4.13	-3.85	-2.61	-2.53	-8.32	-15.34	-14.33	-11.42
None	4.46	3.48	4.54	0.13	4.26	6.49	3.41	5.40	0.36	3.00	5.81	4.38	5.61	-0.19	3.55
Nuclear Periphery	1.34	-2.55	-4.11	-4.37	-1.82	1.40	-2.39	-2.50	-2.69	-1.03	1.96	-3.36	-4.48	-4.77	-2.12
Nuclear Periphery Foci	-0.94	-5.54	-5.47	-4.91	-1.84	2.46	-2.70	-2.54	-2.88	-1.92	0.94	-5.47	-5.63	-5.88	-3.08
Nucleolus	1.43	-1.60	4.93	2.42	2.16	-0.10	-0.69	0.98	0.81	1.28	0.70	-1.72	5.08	2.81	2.93
Nucleus	8.26	-12.38	3.36	-4.26	13.83	5.69	-7.54	3.87	0.39	8.90	10.12	-13.80	4.10	-3.52	15.32
Peroxisomes	-1.17	1.23	-2.62	-1.65	-3.64	-2.59	-1.35	-2.62	-1.32	-2.18	-2.71	-0.50	-3.47	-1.35	-3.18
Vacuole	-0.56	-6.44	-10.17	-9.72	-1.92	-4.50	-18.74	-8.51	-7.56	-1.83	-3.72	-19.12	-13.75	-11.59	3.25
Vacuole Periphery	3.44	10.55	5.30	3.19	-2.16	-3.17	13.97	1.34	2.54	-1.33	0.31	16.16	4.83	4.60	-2.47