Standard name
Human Ortholog
Description UbiB family protein; contains transmembrane domain and mitochondrial targeting sequence; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0.05
Bud 0 0 0.05 0.05 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.15 0.18 0.15 0.12 0.15 0.08 0.12 0.14 0.07 0.1 0.13 0.12 0.35 0.44 0.55 0.37 0.3 0.33 0 0.06 0 0 0.06 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0
Endosome 0 0.05 0 0.05 0.15 0 0 0 0 0 0 0 0.12 0.12 0.19 0.2 0.17 0.16 0.08 0.08 0.09 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.88 0.72 0.74 0.87 0.73 0.88 0.86 0.83 0.95 0.91 0.82 0.86 0.55 0.28 0.16 0.15 0.1 0.09 0.69 0.61 0.66 0.7 0.63 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0 0.07 0 0 0 0 0 0 0 0.06 0 0.07 0.22 0.08 0.1 0 0 0.07 0 0 0
SpindlePole 0 0 0.05 0 0.07 0 0.08 0.09 0 0 0 0 0 0 0 0.09 0.12 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.12 0 0 0.08 0 0 0.05 0 0 0 0 0.09 0.16 0.14 0.16 0.18 0.13 0.06 0.09 0 0 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 3 0 0 0 0 0 0 0 0 0 0 0 0 1 2 7 2 8 16 0 5 7
Bud 3 6 12 7 10 3 16 24 9 11 4 2 1 5 0 7 4 1 1 2 0 1 2 1
Bud Neck 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 2 0 2 2 4 1 3 6
Bud Site 1 0 1 1 0 5 4 4 6 5 0 0 0 0 0 1 0 0
Cell Periphery 0 3 4 3 1 4 2 1 1 2 0 1 6 3 1 14 12 6 0 0 1 0 1 1
Cytoplasm 20 27 34 18 36 30 50 62 22 43 8 7 48 53 89 109 57 41 1 10 1 1 9 3
Endoplasmic Reticulum 0 7 3 3 0 4 2 2 3 10 5 0 2 2 3 20 13 7 3 3 2 1 1 1
Endosome 5 7 7 7 38 4 6 13 0 0 0 0 16 15 30 59 33 20 12 13 26 3 8 8
Golgi 0 1 0 0 0 2 0 0 0 0 0 0 1 0 0 0 1 1 1 3 9 2 1 4
Mitochondria 119 106 170 132 180 328 362 370 300 402 51 48 75 34 25 44 19 11 105 111 196 55 93 90
Nucleus 6 1 1 0 1 8 8 15 7 4 1 0 0 1 0 2 7 2 0 0 0 1 1 0
Nuclear Periphery 0 0 1 0 0 3 1 2 2 10 1 1 0 0 0 0 0 0 3 0 2 0 0 0
Nucleolus 7 0 0 1 0 2 3 3 1 2 0 0 0 0 0 0 0 0 1 0 0 1 2 0
Peroxisomes 1 2 23 6 17 5 5 9 1 0 1 0 8 4 12 66 15 13 2 5 21 2 2 4
SpindlePole 0 2 11 2 16 13 32 38 14 16 2 0 4 0 1 26 23 17 1 1 3 0 3 2
Vac/Vac Membrane 8 18 5 3 20 12 17 21 10 13 1 1 13 19 22 47 34 16 9 16 5 1 9 17
Unique Cell Count 136 147 231 151 246 372 419 444 317 443 62 56 137 121 161 298 189 124 152 184 298 79 148 158
Labelled Cell Count 170 182 275 183 320 423 509 566 376 518 74 60 174 136 183 396 222 142 152 184 298 79 148 158


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.7 4.6 4.2 4.1 3.8 3.2 3.7 3.4 2.9 3.6 3.4 4.9 4.9 4.9 5.6 6.5 6.3 4.4 4.9 5.8
Std Deviation (1e-4) 0.5 0.7 1.2 0.9 1.2 1.2 0.9 1.2 0.9 0.7 1.5 1.3 1.0 1.3 1.4 1.7 1.8 1.2 1.5 1.8 1.9
Intensity Change (Log2) -0.14 -0.15 -0.28 -0.52 -0.31 -0.43 -0.64 -0.35 -0.42 0.11 0.08 0.1 0.29 0.51 0.46 -0.07 0.1 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 5.2 5.6 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8529 1.0118 0.5438 0.9635 0.2925 0.7866 1.8052 2.1708 2.1774 1.4882 2.2336 1.8077 3.5648 3.3797 3.2341 3.2775 3.1271 3.4774
Actin 0.0879 0.0283 0.0305 0.0168 0.182 0.0224 0.056 0.046 0.0203 0.0472 0.0406 0.0074 0.0382 0.0111 0.0153 0.0151 0.0459 0.0178
Bud 0.0025 0.0063 0.0096 0.0018 0.0018 0.0027 0.002 0.0038 0.0052 0.0091 0.0048 0.0028 0.0018 0.0053 0.0055 0.0105 0.0027 0.0016
Bud Neck 0.0017 0.0044 0.0046 0.0013 0.006 0.0236 0.0064 0.0038 0.0013 0.002 0.0011 0.0034 0.0025 0.0051 0.0055 0.0095 0.0014 0.0138
Bud Periphery 0.0037 0.0094 0.0389 0.0067 0.0022 0.0124 0.005 0.0104 0.0141 0.0331 0.007 0.0079 0.0032 0.0098 0.022 0.0392 0.0058 0.0052
Bud Site 0.0073 0.1246 0.0326 0.003 0.0041 0.0041 0.0516 0.0689 0.0112 0.01 0.0117 0.0009 0.0152 0.0475 0.0404 0.0098 0.0097 0.003
Cell Periphery 0.0015 0.0092 0.0061 0.051 0.0002 0.0114 0.0052 0.0075 0.0042 0.0073 0.0005 0.001 0.0027 0.0071 0.0071 0.0117 0.0015 0.0039
Cytoplasm 0.0138 0.067 0.0612 0.1197 0.0201 0.0713 0.0886 0.0886 0.0785 0.1058 0.0232 0.0549 0.0662 0.1191 0.0651 0.0679 0.0207 0.0617
Cytoplasmic Foci 0.1588 0.0455 0.1609 0.0343 0.1907 0.1063 0.0781 0.1033 0.0636 0.0985 0.1394 0.0775 0.1046 0.1101 0.0897 0.0675 0.0921 0.0797
Eisosomes 0.0068 0.0021 0.001 0.0022 0.0007 0.0018 0.0015 0.0036 0.0026 0.0016 0.0003 0.0006 0.0015 0.0014 0.002 0.0018 0.0003 0.0021
Endoplasmic Reticulum 0.0057 0.0202 0.0045 0.0431 0.0008 0.0235 0.0673 0.0119 0.0073 0.0056 0.001 0.0039 0.0144 0.0042 0.0037 0.0058 0.0027 0.0146
Endosome 0.1446 0.1222 0.1108 0.14 0.0485 0.0894 0.1478 0.1006 0.0812 0.0589 0.0816 0.091 0.1534 0.1407 0.1405 0.1598 0.1761 0.1387
Golgi 0.0581 0.0337 0.0672 0.0735 0.0453 0.0749 0.0467 0.0591 0.0557 0.0428 0.0571 0.0558 0.0831 0.0657 0.0726 0.0528 0.0766 0.0871
Lipid Particles 0.0465 0.0415 0.0196 0.0159 0.0929 0.0309 0.0421 0.0125 0.0196 0.0186 0.0107 0.0147 0.0397 0.0216 0.0178 0.0099 0.0097 0.0181
Mitochondria 0.154 0.2561 0.3248 0.4444 0.0188 0.3057 0.1741 0.2428 0.3724 0.4526 0.2931 0.4622 0.322 0.3297 0.4016 0.4244 0.4617 0.4077
None 0.0459 0.1709 0.0695 0.0163 0.1609 0.1418 0.1079 0.1649 0.2025 0.028 0.2579 0.165 0.0869 0.0842 0.0575 0.069 0.0541 0.0931
Nuclear Periphery 0.0399 0.0051 0.0007 0.0029 0.0021 0.0077 0.0179 0.0015 0.0022 0.0018 0.0008 0.001 0.0085 0.0008 0.0008 0.0011 0.0006 0.0033
Nucleolus 0.0121 0.0037 0.0009 0.0005 0.0044 0.0016 0.0018 0.0008 0.0011 0.0006 0.0005 0.0005 0.0006 0.0014 0.001 0.0009 0.0004 0.0007
Nucleus 0.0557 0.0045 0.0012 0.0015 0.0022 0.0017 0.0038 0.0013 0.0034 0.0014 0.0006 0.0008 0.0021 0.002 0.001 0.0014 0.0007 0.0011
Peroxisomes 0.0434 0.0114 0.0347 0.0094 0.1773 0.0427 0.0465 0.041 0.037 0.0503 0.0589 0.037 0.0351 0.0172 0.0299 0.0178 0.0156 0.0318
Punctate Nuclear 0.0107 0.0038 0.0022 0.0006 0.0364 0.0013 0.0187 0.0012 0.0007 0.0034 0.0008 0.0011 0.0015 0.002 0.0041 0.001 0.0006 0.0006
Vacuole 0.0636 0.0238 0.0128 0.0086 0.0017 0.0165 0.0234 0.0217 0.0106 0.0164 0.0048 0.0067 0.0118 0.0105 0.0112 0.0161 0.0145 0.0097
Vacuole Periphery 0.0357 0.0061 0.0057 0.0066 0.0008 0.0064 0.0076 0.0046 0.0051 0.0049 0.0035 0.0041 0.0051 0.0035 0.0054 0.0069 0.0066 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8692 4.4972 4.4494 6.1769 2.1742 5.0279 7.9574 6.8199 8.0989 7.3105
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1985 1622 230 1689 1842 3133 123 1740 3827 4755 353 3429

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1040.47 1134.80 1281.62 1453.69 1066.63 1166.83 1438.65 1522.71 1053.06 1155.90 1336.34 1488.71
Standard Deviation 147.58 191.72 177.84 237.66 148.96 194.28 167.78 220.37 148.83 194.00 189.77 231.63
Intensity Change Log 2 0.125203 0.300733 0.482484 0.129535 0.431656 0.513581 0.127397 0.368491 0.498310

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000154 0.000986 0.000779 0.000208 0.000091 0.000435 0.000906 0.000179 0.000112 0.000794 0.000843
Bud Neck 0.000689 0.000450 0.001516 0.001195 0.000863 0.000774 0.002621 0.001365 0.000773 0.000664 0.001901 0.001281
Bud Site 0.015488 0.014927 0.021292 0.016063 0.016413 0.011662 0.026942 0.020241 0.015933 0.012776 0.023260 0.018183
Cell Periphery 0.000161 0.000130 0.000284 0.000467 0.000201 0.000110 0.000144 0.000543 0.000180 0.000117 0.000235 0.000505
Cytoplasm 0.814329* 0.881317* 0.805975* 0.913140* 0.778159* 0.872458* 0.470502* 0.867676* 0.796920* 0.875480* 0.689082* 0.890070*
Cytoplasmic Foci 0.119530 0.057238 0.074639 0.014251 0.137923 0.057024 0.113799 0.017585 0.128383 0.057097 0.088284 0.015943
Eisosomes 0.000009 0.000003 0.000011 0.000004 0.000013 0.000008 0.000023 0.000005 0.000011 0.000006 0.000015 0.000004
Endoplasmic Reticulum 0.003210 0.001512 0.002612 0.000138 0.004504 0.001057 0.001372 0.000193 0.003833 0.001212 0.002180 0.000165
Endosome 0.003575 0.003718 0.009370 0.001094 0.007359 0.002402 0.020154 0.003301 0.005396 0.002851 0.013128 0.002213
Golgi 0.010675 0.007928 0.004656 0.003398 0.011728 0.007341 0.000803 0.004566 0.011182 0.007541 0.003313 0.003991
Lipid Particles 0.000841 0.000499 0.002353 0.002996 0.001219 0.000434 0.002965 0.002831 0.001023 0.000456 0.002566 0.002913
Mitochondria 0.001427 0.000300 0.000034 0.000694 0.000509 0.000901 0.000009 0.000344 0.000985 0.000696 0.000025 0.000516
Mitotic Spindle 0.000605 0.001660 0.001585 0.003300 0.000334 0.000637 0.000507 0.003836 0.000474 0.000986 0.001210 0.003572
None 0.002753 0.000755 0.000638 0.000498 0.000694 0.001381 0.000076 0.000275 0.001762 0.001167 0.000442 0.000384
Nuclear Periphery 0.000051 0.000018 0.000082 0.000019 0.000039 0.000025 0.000123 0.000041 0.000045 0.000022 0.000096 0.000030
Nuclear Periphery Foci 0.000360 0.000705 0.007245 0.001224 0.000392 0.000100 0.007224 0.001049 0.000376 0.000306 0.007237 0.001135
Nucleolus 0.000129 0.000165 0.000041 0.000137 0.000069 0.000089 0.000065 0.000147 0.000100 0.000115 0.000049 0.000142
Nucleus 0.004877 0.003849 0.010830 0.007343 0.003656 0.005363 0.007103 0.005172 0.004290 0.004846 0.009531 0.006241
Peroxisomes 0.000368 0.000505 0.000376 0.000619 0.000437 0.000387 0.001361 0.000742 0.000401 0.000427 0.000719 0.000681
Vacuole 0.015057 0.019181 0.054273 0.031217 0.028365 0.031773 0.342581* 0.066698 0.021462 0.027477 0.154732 0.049221
Vacuole Periphery 0.005713 0.004987 0.001202 0.001423 0.006915 0.005985 0.001191 0.002486 0.006292 0.005644 0.001198 0.001963

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.12 -6.90 -3.29 -3.27 0.96 7.27 -3.71 -1.88 -2.24 -1.16 5.41 -7.43 -3.29 -3.63 0.03
Bud Neck 3.59 -5.62 -4.27 -5.67 0.66 0.87 -6.16 -4.17 -4.77 3.65 1.71 -8.03 -6.00 -6.87 2.71
Bud Site 0.41 -2.96 -0.43 -0.78 2.50 5.02 -2.00 -1.74 -5.18 1.47 3.99 -3.36 -1.56 -4.72 2.52
Cell Periphery 2.61 -4.04 -5.72 -6.33 -2.81 7.21 3.67 -8.13 -10.80 -9.45 7.17 -2.52 -9.44 -11.53 -6.47
Cytoplasm -10.27 1.45 -14.31 -3.03 -7.51 -15.97 11.84 -10.37 3.86 -14.64 -18.41 7.57 -16.83 0.22 -12.66
Cytoplasmic Foci 14.65 7.13 28.96 14.89 10.67 20.26 3.09 31.87 19.70 10.90 24.94 7.95 42.91 25.06 14.85
Eisosomes 7.19 -2.13 7.19 -1.57 6.01 1.64 -4.18 7.09 0.98 8.21 2.19 -3.77 9.63 0.84 9.57
Endoplasmic Reticulum 2.52 0.65 5.02 4.85 2.47 4.20 4.07 5.45 4.12 5.42 5.00 2.27 7.40 6.18 3.02
Endosome -0.37 -2.76 10.07 6.93 4.01 10.63 -4.04 7.13 -1.64 5.75 9.01 -4.18 10.22 2.90 6.25
Golgi 2.99 4.08 8.23 4.29 0.79 6.66 21.80 8.18 3.71 -3.69 7.07 7.85 11.57 5.81 -0.29
Lipid Particles 3.92 -5.06 -5.84 -6.64 -2.04 9.05 -3.89 -6.79 -10.24 -0.31 9.60 -6.18 -8.57 -10.97 -1.98
Mitochondria 2.66 3.31 1.33 -0.59 -1.15 -1.99 4.04 1.19 2.97 -2.07 1.16 4.23 1.65 0.98 -1.67
Mitotic Spindle -1.54 -3.20 -2.81 -1.59 -1.87 -0.99 -1.38 -3.39 -2.95 -3.12 -1.64 -3.79 -4.35 -3.40 -3.23
None 3.35 3.29 3.73 2.41 0.18 -2.67 5.22 3.25 4.60 -3.13 1.72 3.71 4.30 4.76 0.03
Nuclear Periphery 1.64 -1.91 1.03 -2.30 5.19 0.91 -5.26 -0.84 -2.46 5.44 1.73 -4.25 0.30 -2.78 6.69
Nuclear Periphery Foci -2.54 -8.86 -8.43 -4.93 7.21 9.37 -6.20 -7.23 -9.92 5.41 1.25 -10.82 -11.08 -11.22 9.13
Nucleolus -0.50 2.22 -0.56 0.15 -3.64 -3.05 0.46 -6.95 -5.01 -6.67 -0.52 2.41 -2.18 -1.42 -6.28
Nucleus 1.43 -4.12 -5.28 -6.72 1.81 -5.91 -3.46 -7.35 -3.97 0.47 -1.64 -5.21 -8.27 -7.63 2.08
Peroxisomes -0.92 -0.28 -2.77 -0.54 -2.30 1.20 -2.92 -2.94 -3.34 1.90 -0.54 -2.72 -4.06 -2.74 0.34
Vacuole -2.53 -6.53 -12.92 -9.82 3.11 -1.81 -13.01 -14.71 -13.74 10.72 -4.59 -11.61 -18.92 -15.48 8.21
Vacuole Periphery 1.03 8.02 7.69 6.21 -0.23 1.20 8.51 5.99 6.20 -2.22 1.25 11.42 9.36 9.16 -1.72