Standard name
Description ATPase component of heat shock protein Hsp90 chaperone complex; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; plays a role in prion propagation and determining prion variants; binds unfolded proteins; member of Hsp110 subclass of HSP70 proteins; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0 0 0.08 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.98 0.98 0.97 0.94 0.9 0.9 0.88 0.86 0.99 0.98 1.0 0.97 0.96 0.99 0.96 0.96 0.96 0.89 0.89 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.08 0.08 0.1 0.09 0 0 0 0 0 0.05 0 0 0 0 0.05 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 4 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 2 3 2 1 3 2 2 12 18 6 1 0 1 3 1 0 0 2 2 1 2 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 13 12 16 24 30 30 44 25 43 2 0 2 62 44 28 1 0 0 0 0 0
Cytoplasm 159 188 312 375 502 528 709 499 639 584 584 331 427 469 761 472 564 157 189 309 228 326 425
Endoplasmic Reticulum 1 0 0 4 9 15 43 42 59 65 64 3 8 2 22 19 26 1 0 1 9 17 25
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 1 2 2 3 4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 1 0 0 1 2 3 1 1 0 1 8 2 3 0 0 2 9 4 7
Unique Cell Count 160 189 315 381 513 546 756 553 713 663 676 335 434 471 787 490 571 164 197 324 257 365 474
Labelled Cell Count 160 192 328 394 529 570 784 575 758 700 700 338 436 476 860 544 621 164 197 324 257 365 474


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 117.8 98.4 98.6 100.8 100.8 87.9 84.0 78.1 73.0 68.0 83.2 125.0 127.1 121.9 90.4 94.2 87.3 103.8 104.5 111.4
Std Deviation (1e-4) 31.2 31.6 26.2 26.5 22.0 20.0 19.2 20.7 19.2 19.3 22.2 31.0 35.5 36.7 29.0 30.5 25.9 26.7 32.0 30.9
Intensity Change (Log2) 0.03 0.03 -0.17 -0.23 -0.34 -0.43 -0.54 -0.24 0.34 0.37 0.31 -0.13 -0.07 -0.18 0.07 0.08 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -0.8 0.2 -0.1 0.8 1.3 -0.3 1.4 -3.0 -4.3 -3.7 2.2 2.6 0.5
Cytoplasm -0.7 -1.3 -2.1 -3.7 -5.0 -5.3 -5.8 -6.3 -0.3 -0.8 0.9 -2.2 -2.4 -0.4
Endoplasmic Reticulum 0 0 3.0 4.3 5.0 5.3 5.8 5.6 0 0 0 3.0 3.5 3.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sse1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available