Standard name
Human Ortholog
Description Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.98 0.99 0.97 0.92 0.91 0.9 0.74 0.77 0.7 0.72 0.98 0.98 1.0 0.94 0.94 0.88 0.71 0.96 0.87 0.7 0.74 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.08 0.35 0.32 0.43 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.14 0 0.05 0.13 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 0 2 6 1 0 0 2 3 1 0 0 0 0 1 0 0 1 0 2 1
Bud 0 0 4 1 2 0 2 0 6 8 8 5 0 2 1 0 0 0 0 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 8 3 7 5 13 15 19 6 15 11 12 15 7 9 7 18 18 14 0 0 0 0 0 0
Cytoplasm 154 267 215 226 366 320 508 333 264 279 195 255 274 369 552 346 173 138 138 268 198 140 411 350
Endoplasmic Reticulum 2 8 3 2 3 10 13 14 1 1 3 4 3 3 2 12 4 7 7 1 2 18 68 47
Endosome 0 1 0 0 2 0 0 0 2 2 0 6 0 0 0 0 0 1 0 0 1 0 1 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 3 0 0 1 3 3
Mitochondria 3 3 1 3 10 30 56 28 126 116 120 104 2 0 2 3 1 2 4 1 6 5 16 9
Nucleus 6 0 1 4 1 0 3 4 3 0 1 3 2 1 1 6 2 4 3 0 2 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 3 1 1 1 3 5 1 9 10 12 6 8 1 3 0 19 5 8 27 5 10 25 41 28
Unique Cell Count 163 276 219 228 378 348 557 372 356 362 280 353 281 375 553 368 185 157 194 281 227 200 558 450
Labelled Cell Count 177 284 234 244 400 382 609 395 427 430 349 404 291 388 565 404 204 176 194 281 227 200 558 450


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 8.3 7.8 7.3 7.9 6.3 6.2 6.3 5.4 5.2 5.1 5.6 8.0 8.1 8.1 11.7 12.6 11.4 7.7 7.6 8.0
Std Deviation (1e-4) 1.2 1.1 1.0 1.1 1.1 1.1 1.0 1.1 1.2 1.0 0.9 0.8 1.1 1.0 1.0 1.9 1.7 1.8 1.2 1.2 1.3
Intensity Change (Log2) -0.1 0.03 -0.3 -0.32 -0.3 -0.52 -0.57 -0.61 -0.47 0.05 0.07 0.06 0.6 0.7 0.56 -0.01 -0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 0.2 0.7 0.1 0 0.6 -0.1 0.6 0.6 -0.5 -0.6 0 1.0 2.7 2.4
Cytoplasm 0.9 -1.0 -3.1 -3.5 -3.9 -7.5 -6.9 -8.3 -7.9 -0.5 0.2 2.6 -2.4 -2.4 -4.1
Endoplasmic Reticulum 0 0 1.2 0 1.7 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 4.6 3.8 9.8 9.2 11.0 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3722 6.3466 6.0958 5.9886 5.7027 6.0607 2.3235 4.5962 3.7684 3.1739 2.8324 3.4878 4.1302 6.1252 5.4265 5.012 3.9653 5.1833
Actin 0.0305 0.001 0.0091 0.0007 0.039 0.001 0.0075 0.0026 0.0009 0.0013 0.0047 0.0009 0.0366 0.0119 0.0119 0.0071 0.0048 0.0089
Bud 0.0024 0.0003 0.0006 0.0005 0.0002 0.0004 0.0005 0.0004 0.0005 0.0196 0.0027 0.0002 0.0058 0.023 0.022 0.0094 0.0009 0.0026
Bud Neck 0.0209 0.0003 0.0004 0.0006 0.0004 0.0009 0.0007 0.0002 0.0003 0.0006 0.0016 0.0007 0.0009 0.0011 0.0014 0.0011 0.0007 0.0023
Bud Periphery 0.0018 0.0002 0.001 0.0004 0.0002 0.0014 0.0006 0.0001 0.0001 0.0148 0.0054 0.0001 0.0044 0.0103 0.0102 0.0073 0.0007 0.0017
Bud Site 0.0063 0.0037 0.0015 0.0009 0.0002 0.0002 0.0008 0.0013 0.0015 0.0011 0.0016 0.0002 0.0081 0.0605 0.0203 0.002 0.0008 0.0007
Cell Periphery 0.001 0.0015 0.0014 0.0004 0.0004 0.0008 0.0002 0.0002 0.0001 0.0004 0.0002 0 0.0007 0.0012 0.0007 0.0003 0.0002 0.0002
Cytoplasm 0.6047 0.9346 0.8677 0.6171 0.7713 0.8898 0.6843 0.9424 0.9035 0.7376 0.7124 0.8812 0.6825 0.803 0.794 0.7868 0.7132 0.7985
Cytoplasmic Foci 0.0368 0.0096 0.0164 0.0665 0.0174 0.014 0.054 0.0175 0.0205 0.0269 0.0532 0.0248 0.0448 0.0172 0.0355 0.0349 0.0357 0.0385
Eisosomes 0.0008 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0781 0.011 0.017 0.0879 0.0067 0.0185 0.0292 0.0093 0.0047 0.0039 0.0052 0.0045 0.0218 0.0031 0.0145 0.0158 0.0062 0.0128
Endosome 0.0447 0.0142 0.0281 0.1709 0.0719 0.036 0.1251 0.0133 0.0465 0.1089 0.1258 0.0485 0.035 0.0067 0.0316 0.0408 0.0651 0.0465
Golgi 0.0112 0.0024 0.0058 0.007 0.0099 0.0045 0.0226 0.0046 0.0061 0.0118 0.0163 0.0119 0.0052 0.0007 0.0053 0.0025 0.0056 0.0055
Lipid Particles 0.0219 0.0004 0.001 0.0006 0.0178 0.0001 0.0081 0.0001 0.0003 0.0027 0.0051 0.0048 0.0179 0.0005 0.0015 0.0019 0.0363 0.0058
Mitochondria 0.0105 0.0005 0.0057 0.0158 0.0015 0.0146 0.0173 0.0011 0.0015 0.0445 0.0218 0.0066 0.0355 0.0029 0.0033 0.0046 0.053 0.019
None 0.0726 0.0147 0.026 0.0078 0.0285 0.0122 0.0116 0.0027 0.0053 0.0056 0.0031 0.0009 0.0542 0.0435 0.024 0.0322 0.017 0.0299
Nuclear Periphery 0.0054 0.0006 0.0009 0.0064 0.0033 0.0007 0.0106 0.0005 0.001 0.0009 0.0027 0.0005 0.0104 0.0011 0.0025 0.013 0.0119 0.0076
Nucleolus 0.0008 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0001 0.0002 0 0.0012 0.0007 0.0008 0.001 0.0005 0.0016
Nucleus 0.0039 0.0006 0.0008 0.0019 0.0007 0.0006 0.0044 0.0004 0.0008 0.001 0.0016 0.0005 0.0059 0.0039 0.0081 0.01 0.0032 0.0053
Peroxisomes 0.0249 0.0001 0.0088 0.0003 0.0116 0.0001 0.0025 0.0005 0.0001 0.0027 0.0046 0.0084 0.0045 0.0009 0.0011 0.0008 0.0299 0.0013
Punctate Nuclear 0.0131 0.0001 0.0002 0.0006 0.0049 0.0001 0.0084 0.0001 0.0003 0.0002 0.0094 0.0002 0.0051 0.0018 0.0024 0.0076 0.0017 0.004
Vacuole 0.0063 0.0039 0.0063 0.0101 0.012 0.0027 0.0079 0.0022 0.0048 0.0122 0.0167 0.0037 0.0158 0.0055 0.0076 0.0165 0.0078 0.0049
Vacuole Periphery 0.0014 0.0003 0.001 0.0035 0.0017 0.0013 0.0032 0.0005 0.0011 0.0032 0.0058 0.0014 0.0034 0.0007 0.0012 0.0042 0.0045 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.6694 42.9364 35.018 36.7563 28.3317 25.5156 40.3818 41.4435 37.6858 40.7417
Translational Efficiency 1.471 1.1703 1.4153 1.6108 1.7873 1.8065 1.1032 1.1034 1.1845 1.1644

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency