Standard name
Human Ortholog
Description Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0 0 0 – 0 0 0 0 0 0.1 0.1 0 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.13 0.24 0.12 0.15 0.07 0.1 – 0 0 0 0 0.2 0.16 0.28 0.18 0.2 0.22 0.1 0.13 0.13 0.12 0.1 0.06
Golgi 0 0 0 0 0.07 0 0 – 0 0 0 0 0.14 0.14 0.12 0.11 0.1 0.09 0 0 0 0 0 0
Mitochondria 0.91 0.65 0.33 0.67 0.52 0.75 0.74 – 0.9 0.85 0.96 0.92 0.54 0.43 0.25 0.13 0.16 0.13 0.67 0.65 0.58 0.78 0.7 0.65
Nucleus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.1 0 0.13 0.09 0 0 – 0 0 0 0 0.06 0.08 0.24 0.23 0.23 0.23 0.06 0.06 0.08 0 0 0
SpindlePole 0.1 0.1 0.33 0.13 0.16 0.07 0.08 – 0.06 0.07 0 0 0.12 0.08 0.24 0.3 0.21 0.25 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0.2 0.24 0 0.14 0.09 0.13 – 0.1 0.07 0 0.06 0.08 0.07 0.11 0.2 0.22 0.2 0 0 0.06 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 6 2 0 0 – 0 1 1 0 4 8 0 2 2 1 3 3 2 0 3 7
Bud 2 0 1 8 7 7 11 – 9 12 9 10 1 5 7 6 10 6 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 2 1 0 0 0 0 1 2
Bud Site 0 0 0 0 0 2 2 – 5 3 6 3 0 1 0 2 1 4 – – – – – –
Cell Periphery 0 5 1 5 7 2 5 – 2 5 3 1 3 7 5 12 11 7 0 0 0 0 0 1
Cytoplasm 3 8 1 15 12 8 15 – 5 6 1 5 3 10 16 7 15 15 0 1 0 0 0 5
Endoplasmic Reticulum 1 0 0 2 4 0 2 – 0 5 1 3 1 3 1 2 7 7 0 0 1 0 0 1
Endosome 6 17 11 22 51 16 37 – 9 11 0 5 17 16 46 37 46 40 13 18 12 6 18 13
Golgi 0 2 1 8 24 0 3 – 1 0 0 3 12 14 19 23 24 17 4 6 1 1 4 9
Mitochondria 107 85 15 125 180 177 276 – 252 382 247 297 45 44 41 28 37 24 84 92 57 38 132 152
Nucleus 0 1 0 1 2 12 15 – 4 6 3 8 1 2 0 2 7 5 0 0 0 0 0 1
Nuclear Periphery 1 1 0 0 2 3 11 – 11 33 18 15 1 0 0 1 1 0 1 0 0 0 0 1
Nucleolus 7 1 1 0 2 12 11 – 8 18 11 11 1 0 1 2 6 4 1 0 0 0 0 0
Peroxisomes 4 13 2 24 30 7 16 – 3 3 2 2 5 8 40 48 53 42 7 7 7 0 6 8
SpindlePole 12 13 15 25 54 17 29 – 17 31 7 13 10 8 39 62 50 45 3 3 5 0 1 4
Vac/Vac Membrane 5 26 11 4 49 21 48 – 29 33 11 18 7 7 18 43 51 37 0 3 6 1 13 20
Unique Cell Count 117 131 46 187 343 236 374 281 451 258 324 83 103 165 210 234 183 126 142 99 50 190 236
Labelled Cell Count 148 174 59 245 426 284 481 355 549 320 394 111 133 233 279 323 255 126 142 99 50 190 236


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.9 6.1 6.3 6.9 5.5 5.9 – 5.3 5.4 5.0 5.3 6.4 6.5 6.4 7.6 7.9 7.5 – 6.1 6.2
Std Deviation (1e-4) 0.6 0.9 1.0 1.7 2.3 1.3 1.3 – 0.9 1.1 1.2 1.2 1.1 0.9 1.1 1.5 2.1 2.0 – 1.2 1.2
Intensity Change (Log2) – – – 0.06 0.18 -0.15 -0.04 – -0.21 -0.17 -0.27 -0.19 0.08 0.11 0.08 0.33 0.39 0.31 – 0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -2.1 -1.6 0 -2.8 – 0 0 0 0 -0.5 -1.2 0.5 -1.0 -0.7 -0.3
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 2.5 5.6 5.7 – 9.3 8.4 11.4 10.3 2.4 1.2 -1.1 -3.2 -2.7 -3.1
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.6 0 0 0 – 0 0 0 0 0 0 3.0 2.9 2.9 2.9
SpindlePole -3.1 -2.8 -5.0 0 – 0 0 0 0 -2.8 -3.9 -1.2 -0.4 -1.7 -1.1
Vacuole 0 -1.7 -2.9 -2.0 – -2.6 0 0 0 -2.4 -3.0 -2.3 -0.5 -0.3 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0324 0.0169 0.0361 0.0033 0.0006 0.0364 – – – – – – 0.0392 0.01 0.0116 0.0186 0.0101 0.0108
Bud 0.0039 0.0069 0.004 0.0023 0.0001 0.0014 – – – – – – 0.0008 0.0064 0.0024 0.0016 0.0077 0.0007
Bud Neck 0.0015 0.0015 0.0022 0.0267 0.0001 0.0039 – – – – – – 0.0044 0.002 0.0014 0.0011 0.0015 0.0096
Bud Periphery 0.005 0.0112 0.0114 0.0055 0.0002 0.0057 – – – – – – 0.0016 0.0047 0.0068 0.0035 0.0241 0.0016
Bud Site 0.0088 0.0403 0.0102 0.0029 0.0001 0.0026 – – – – – – 0.0041 0.0156 0.0139 0.003 0.0021 0.0013
Cell Periphery 0.0011 0.0013 0.0022 0.0019 0.0002 0.0041 – – – – – – 0.0007 0.0012 0.0013 0.0004 0.0004 0.0005
Cytoplasm 0.0131 0.0113 0.0073 0.0026 0.0001 0.0188 – – – – – – 0.0153 0.0258 0.0168 0.0035 0.0045 0.0121
Cytoplasmic Foci 0.0582 0.0613 0.0467 0.0253 0.0024 0.0321 – – – – – – 0.0577 0.061 0.0416 0.0389 0.053 0.0427
Eisosomes 0.0006 0.0005 0.0015 0.0016 0.0001 0.0009 – – – – – – 0.0006 0.0002 0.0005 0.0004 0.0003 0.0003
Endoplasmic Reticulum 0.0073 0.003 0.0027 0.0005 0.0003 0.0036 – – – – – – 0.0071 0.0034 0.002 0.0023 0.0012 0.0029
Endosome 0.1145 0.0698 0.066 0.0728 0.032 0.0635 – – – – – – 0.1691 0.1782 0.1068 0.1026 0.1291 0.0966
Golgi 0.0948 0.0813 0.0524 0.0561 0.0146 0.075 – – – – – – 0.0662 0.0822 0.0476 0.0377 0.0646 0.055
Lipid Particles 0.04 0.0424 0.0322 0.0162 0.0237 0.0332 – – – – – – 0.051 0.0234 0.0284 0.0114 0.0207 0.0289
Mitochondria 0.4517 0.5436 0.6117 0.6378 0.8823 0.637 – – – – – – 0.4353 0.4766 0.6415 0.7026 0.6257 0.6674
None 0.0122 0.0046 0.0394 0.0678 0.0001 0.01 – – – – – – 0.0293 0.0199 0.0064 0.0014 0.0028 0.008
Nuclear Periphery 0.0095 0.0031 0.0009 0.0007 0.0008 0.0015 – – – – – – 0.0113 0.002 0.0047 0.0017 0.0013 0.0037
Nucleolus 0.0056 0.0011 0.0013 0.0053 0.0003 0.0005 – – – – – – 0.002 0.0043 0.0009 0.0004 0.0004 0.0012
Nucleus 0.0064 0.0038 0.0009 0.0024 0.0002 0.0009 – – – – – – 0.004 0.002 0.0072 0.0005 0.0007 0.0011
Peroxisomes 0.0966 0.0725 0.0468 0.0336 0.0133 0.0597 – – – – – – 0.0606 0.0462 0.0335 0.0427 0.0346 0.0338
Punctate Nuclear 0.0044 0.001 0.0007 0.0024 0 0.0008 – – – – – – 0.0106 0.0008 0.0012 0.0006 0.0007 0.0026
Vacuole 0.0179 0.0165 0.0132 0.0253 0.0089 0.0052 – – – – – – 0.0197 0.0255 0.0156 0.0136 0.0085 0.0119
Vacuole Periphery 0.0144 0.0061 0.0104 0.007 0.0198 0.0031 – – – – – – 0.0094 0.0087 0.0078 0.0117 0.0059 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0927 10.9764 6.2938 9.878 12.3017 7.8094 12.3594 12.4275 14.8402 13.9164
Translational Efficiency 0.5775 0.4192 0.613 0.548 0.3502 0.8165 0.5496 0.6152 0.4957 0.4968

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1955 904 1729 1500 1868 1456 667 2589 3823 2360 2396 4089

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.58 672.95 933.50 944.60 729.69 742.12 996.17 931.44 678.50 715.62 950.95 936.27
Standard Deviation 73.92 113.00 111.22 139.52 93.74 105.57 118.76 120.48 97.94 113.57 116.80 127.95
Intensity Change Log 2 — 0.096110 0.568260 0.585314 — 0.024369 0.449108 0.352179 — 0.058041 0.505522 0.464859

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001032 0.002540 0.007979 0.023811 0.000640 0.001049 0.006591 0.008107 0.000840 0.001620 0.007593 0.013868
Bud Neck 0.042529 0.011416 0.003106 0.003986 0.005159 0.003747 0.002344 0.002800 0.024269 0.006684 0.002894 0.003235
Bud Site 0.043955 0.017848 0.027056 0.042824 0.007922 0.006042 0.025515 0.028675 0.026348 0.010564 0.026627 0.033866
Cell Periphery 0.000761 0.000605 0.000348 0.000775 0.000232 0.000244 0.000313 0.000422 0.000503 0.000382 0.000338 0.000551
Cytoplasm 0.028576 0.007457 0.004434 0.009010 0.003130 0.001753 0.005043 0.008871 0.016143 0.003938 0.004603 0.008922
Cytoplasmic Foci 0.115514 0.059259 0.076733 0.065413 0.043804 0.048581 0.129783 0.037345 0.080475 0.052671 0.091501 0.047642
Eisosomes 0.000135 0.000122 0.000099 0.000142 0.000063 0.000065 0.000087 0.000095 0.000100 0.000087 0.000096 0.000112
Endoplasmic Reticulum 0.001070 0.000717 0.001946 0.001347 0.000312 0.000252 0.001372 0.000440 0.000700 0.000430 0.001786 0.000773
Endosome 0.097576 0.119194 0.097444 0.082716 0.103060 0.127369 0.118599 0.064434 0.100256 0.124237 0.103334 0.071141
Golgi 0.087201 0.094234 0.111663* 0.125049* 0.107772 0.119152* 0.189133* 0.095682 0.097253 0.109608 0.133229* 0.106455
Lipid Particles 0.012893 0.014925 0.002067 0.001622 0.005732 0.004612 0.001353 0.000878 0.009394 0.008562 0.001868 0.001151
Mitochondria 0.436172* 0.525764* 0.494509* 0.463325* 0.644834* 0.591375* 0.347703* 0.634435* 0.538129* 0.566243* 0.453641* 0.571665*
Mitotic Spindle 0.014102 0.014132 0.061399 0.067263 0.008731 0.012122 0.074082 0.041203 0.011478 0.012892 0.064930 0.050763
None 0.004572 0.002000 0.000260 0.000225 0.000313 0.000168 0.000211 0.000226 0.002491 0.000870 0.000247 0.000226
Nuclear Periphery 0.000211 0.000113 0.000589 0.000600 0.000267 0.000078 0.000703 0.000301 0.000238 0.000091 0.000620 0.000411
Nuclear Periphery Foci 0.001924 0.000951 0.001443 0.000725 0.000460 0.000248 0.000678 0.000247 0.001209 0.000517 0.001230 0.000422
Nucleolus 0.003682 0.004232 0.000175 0.000114 0.000519 0.000538 0.000175 0.000237 0.002136 0.001953 0.000175 0.000192
Nucleus 0.005264 0.002191 0.000822 0.000223 0.000605 0.000605 0.000375 0.000221 0.002988 0.001212 0.000698 0.000222
Peroxisomes 0.068331 0.105805* 0.049937 0.069915 0.044111 0.059961 0.047525 0.036727 0.056497 0.077522 0.049265 0.048901
Vacuole 0.020585 0.008876 0.034141 0.025720 0.009232 0.011124 0.024368 0.022441 0.015038 0.010263 0.031420 0.023644
Vacuole Periphery 0.013912 0.007620 0.023850 0.015195 0.013102 0.010916 0.024049 0.016213 0.013517 0.009654 0.023906 0.015840

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.32 -7.77 -10.33 -9.44 -6.76 -2.31 -4.99 -9.18 -8.58 -1.05 -2.67 -9.55 -13.40 -12.42 -5.30
Bud Neck 9.95 15.81 15.33 3.74 -1.57 1.31 2.83 2.38 1.19 -0.66 10.98 15.30 14.95 3.80 -0.78
Bud Site 7.88 5.33 0.20 -7.10 -4.68 1.38 -5.67 -10.69 -11.73 -0.77 9.14 -0.37 -3.85 -12.99 -3.23
Cell Periphery 1.66 5.64 -0.05 -0.70 -1.83 -0.30 -1.76 -4.77 -3.87 -1.91 2.56 3.57 -0.51 -1.81 -2.29
Cytoplasm 9.10 12.08 9.24 -1.11 -5.56 2.37 -2.41 -7.32 -11.32 -4.10 10.42 10.39 6.14 -7.48 -7.88
Cytoplasmic Foci 11.70 8.58 10.94 -1.39 2.99 -1.68 -14.09 3.02 4.58 15.46 10.07 -3.04 12.91 2.46 15.14
Eisosomes 0.90 5.67 -0.77 -1.32 -6.01 -0.51 -4.30 -7.63 -6.88 -1.45 2.15 1.05 -2.79 -4.13 -3.91
Endoplasmic Reticulum 1.45 -2.46 -0.94 -2.27 1.58 1.01 -3.51 -1.71 -2.42 3.00 2.30 -4.23 -0.59 -3.03 3.92
Endosome -3.38 0.01 3.13 5.88 3.40 -4.01 -2.34 8.57 12.16 9.33 -5.47 -0.83 9.06 13.34 10.02
Golgi -1.27 -4.96 -6.69 -4.64 -2.23 -2.01 -9.34 2.53 4.50 11.09 -3.09 -8.30 -2.56 0.80 6.05
Lipid Particles -0.80 9.36 9.47 5.37 0.66 0.96 4.84 5.28 3.87 1.37 0.65 9.89 11.00 6.59 1.91
Mitochondria -5.86 -4.84 -2.14 3.86 2.36 4.25 18.98 1.01 -3.60 -19.00* -2.91 8.82 -3.99 -0.52 -12.54
Mitotic Spindle 0.02 -14.12 -13.47 -12.85 -1.17 -2.07 -10.58 -13.36 -10.75 4.99 -1.07 -18.25 -18.38 -16.49 4.04
None 2.13 5.78 5.89 1.81 0.67 2.00 1.37 1.23 -3.39 -0.60 3.06 5.79 5.95 1.71 0.61
Nuclear Periphery 2.04 -2.85 -1.99 -2.51 -0.05 1.43 -1.49 -0.29 -2.43 1.39 2.15 -2.83 -1.57 -3.48 1.34
Nuclear Periphery Foci 1.57 0.77 1.98 0.65 1.26 0.93 -1.08 1.38 0.57 3.55 2.07 0.09 2.53 0.92 2.19
Nucleolus -0.36 4.87 4.95 3.41 1.20 0.03 2.51 1.55 0.98 -0.60 0.36 5.23 5.07 3.52 -0.38
Nucleus 3.56 6.02 7.48 3.84 2.07 0.08 1.10 1.37 0.86 0.38 4.00 5.49 7.38 3.34 1.82
Peroxisomes -5.25 4.16 -0.30 4.73 -3.87 -3.36 -0.60 2.08 5.23 2.03 -5.22 2.13 2.59 7.13 0.17
Vacuole 7.26 -6.15 -2.35 -8.04 3.18 -1.85 -7.19 -8.96 -7.22 0.79 4.89 -10.24 -6.61 -10.68 4.30
Vacuole Periphery 5.24 -5.94 -0.93 -5.59 4.81 1.80 -4.36 -2.39 -4.02 3.05 4.41 -7.63 -2.42 -6.40 5.65
Standard name
Human Ortholog
Description Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
Localization
Cell Percentages mitochondrion (80%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Msd1

Msd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available