Standard name | |
---|---|
Human Ortholog | |
Description | Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec66p, and Sec72p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 12 | 16 | 10 | 18 | 28 | 35 | 15 | – | 34 | 23 | 24 | 26 | 47 | 21 | 23 | 21 | 19 | 2 | 5 | 5 | 6 | 16 | 13 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 2 | 6 | – | 1 | 3 | 4 | 5 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 18 | 13 | 10 | 14 | 20 | 25 | 28 | – | 43 | 38 | 39 | 47 | 103 | 140 | 81 | 64 | 51 | 1 | 4 | 4 | 3 | 8 | 17 |
Cytoplasm | – | 25 | 8 | 2 | 15 | 22 | 33 | 34 | – | 43 | 28 | 37 | 47 | 25 | 35 | 31 | 23 | 21 | 1 | 3 | 1 | 3 | 6 | 5 |
Endoplasmic Reticulum | – | 184 | 118 | 41 | 157 | 191 | 220 | 145 | – | 231 | 127 | 125 | 268 | 421 | 448 | 1 | 5 | 0 | 30 | 191 | 123 | 258 | 390 | 404 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Golgi | – | 0 | 5 | 10 | 14 | 0 | 3 | 1 | – | 2 | 0 | 0 | 34 | 24 | 8 | 37 | 24 | 57 | 0 | 0 | 0 | 3 | 5 | 5 |
Mitochondria | – | 0 | 14 | 8 | 9 | 36 | 84 | 73 | – | 69 | 91 | 74 | 0 | 2 | 1 | 13 | 10 | 8 | 2 | 0 | 0 | 2 | 5 | 9 |
Nucleus | – | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 5 | 3 | 2 | 1 | 9 | 5 | – | 6 | 6 | 10 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 2 | 1 | 3 | 8 | 4 | 17 | 9 | – | 41 | 8 | 6 | 54 | 11 | 5 | 4 | 2 | 27 | 0 | 2 | 0 | 3 | 6 | 10 |
Unique Cell Count | 207 | 140 | 54 | 186 | 243 | 305 | 240 | 365 | 227 | 219 | 376 | 507 | 541 | 166 | 120 | 130 | 42 | 214 | 138 | 287 | 448 | 475 | ||
Labelled Cell Count | 241 | 180 | 87 | 238 | 303 | 429 | 317 | 472 | 324 | 319 | 481 | 636 | 661 | 192 | 149 | 183 | 42 | 214 | 138 | 287 | 448 | 475 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 22.0 | 15.2 | 15.3 | 13.4 | 11.1 | 10.2 | 10.0 | – | 10.0 | 9.6 | 9.9 | 20.2 | 20.7 | 20.0 | 21.9 | 22.8 | 23.0 | 21.8 | 22.7 | 22.5 |
Std Deviation (1e-4) | – | 4.4 | 2.9 | 2.2 | 2.7 | 1.7 | 2.0 | 1.8 | – | 2.3 | 2.0 | 2.2 | 2.9 | 3.5 | 3.3 | 4.2 | 4.6 | 4.3 | 3.9 | 4.4 | 4.1 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.19 | -0.46 | -0.59 | -0.61 | – | -0.62 | -0.67 | -0.63 | 0.41 | 0.44 | 0.39 | 0.52 | 0.58 | 0.59 | 0.52 | 0.57 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.3 | -0.5 | 0 | 0 | -1.8 | – | -0.7 | -0.4 | -0.1 | -1.7 | -0.8 | -3.5 | 0.6 | 1.4 | 0.8 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | -0.6 | -0.4 | -0.4 | 0.7 | – | 0.8 | 2.0 | 2.2 | 1.0 | 3.0 | 4.2 | 7.5 | 7.8 | 5.8 |
Cytoplasm | 0 | 0.8 | 1.2 | 1.7 | 2.5 | – | 2.0 | 2.1 | 3.1 | 2.2 | -0.4 | 0.3 | 3.4 | 3.3 | 2.8 |
Endoplasmic Reticulum | -1.4 | 0 | -1.4 | -2.8 | -4.9 | – | -4.6 | -5.6 | -5.4 | -3.0 | -0.4 | -0.4 | -15.0 | -12.9 | -14.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.1 | 0.6 | 0 | 4.7 | 4.2 | 7.9 |
Mitochondria | 0.9 | -1.8 | 1.3 | 4.1 | 4.6 | – | 2.4 | 6.2 | 5.1 | 0 | 0 | 0 | -0.7 | -0.5 | -1.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.4 | 0 | – | 3.8 | 0 | 0 | 4.5 | 0 | 0 | 0 | 0 | 5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.9828 | 16.5588 | 16.5446 | 16.7797 | 15.892 | 16.0794 | 19.8902 | 20.6201 | 19.7866 | 18.6998 | 19.7706 | 19.6921 | 18.6704 | 16.7111 | 17.684 | 15.925 | 16.4171 | 16.837 |
Actin | 0.0118 | 0.009 | 0.0217 | 0.0122 | 0.0074 | 0.0186 | 0.0369 | 0.0006 | 0.0104 | 0.001 | 0.0001 | 0.0046 | 0.0041 | 0.0018 | 0.0005 | 0.0218 | 0.0001 | 0.0006 |
Bud | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Bud Neck | 0.0003 | 0.0007 | 0.0025 | 0.001 | 0.0083 | 0.0007 | 0.0006 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0012 | 0.0002 | 0.0005 | 0.0001 | 0.0017 |
Bud Periphery | 0.0002 | 0.001 | 0.0007 | 0.0001 | 0.0006 | 0.0002 | 0.0012 | 0.0005 | 0.0003 | 0.0012 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 |
Bud Site | 0.0004 | 0.0029 | 0.0037 | 0 | 0.0136 | 0.0001 | 0.0013 | 0.0007 | 0.0053 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0059 | 0 | 0.0004 | 0 | 0 |
Cell Periphery | 0.0022 | 0.022 | 0.0066 | 0.0047 | 0.0025 | 0.0009 | 0.0064 | 0.0169 | 0.0079 | 0.005 | 0.002 | 0.0016 | 0.0039 | 0.0099 | 0.0029 | 0.0016 | 0.0032 | 0.0015 |
Cytoplasm | 0.019 | 0.0351 | 0.017 | 0.0028 | 0.0423 | 0.0061 | 0.0078 | 0.0095 | 0.0052 | 0.0111 | 0.0017 | 0.0019 | 0.0052 | 0.0347 | 0.0082 | 0.0143 | 0.0022 | 0.0025 |
Cytoplasmic Foci | 0.0069 | 0.0021 | 0.0076 | 0.0023 | 0.0276 | 0.0051 | 0.0054 | 0.0004 | 0.0046 | 0.0036 | 0.0001 | 0.0002 | 0.0031 | 0.0019 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Eisosomes | 0.0006 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0011 | 0.0003 | 0.0013 | 0.0036 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.8923 | 0.8864 | 0.9023 | 0.9236 | 0.8287 | 0.9225 | 0.85 | 0.9487 | 0.9211 | 0.9476 | 0.9903 | 0.9738 | 0.9386 | 0.9232 | 0.977 | 0.8992 | 0.9834 | 0.979 |
Endosome | 0.0165 | 0.0063 | 0.0059 | 0.0076 | 0.0147 | 0.0102 | 0.0151 | 0.0018 | 0.0086 | 0.005 | 0.0005 | 0.0027 | 0.0044 | 0.0011 | 0.0009 | 0.0038 | 0.001 | 0.0016 |
Golgi | 0.0087 | 0.01 | 0.0069 | 0.0371 | 0.0138 | 0.0166 | 0.0147 | 0.0029 | 0.0076 | 0.0017 | 0.0007 | 0.003 | 0.003 | 0.0021 | 0.0011 | 0.0046 | 0.0021 | 0.0007 |
Lipid Particles | 0.0067 | 0.0076 | 0.0079 | 0.0018 | 0.0076 | 0.0045 | 0.0103 | 0.0082 | 0.0109 | 0.0022 | 0.0006 | 0.0008 | 0.0035 | 0.0068 | 0.0005 | 0.0013 | 0.0009 | 0.0005 |
Mitochondria | 0.0013 | 0.0005 | 0.0058 | 0.0008 | 0.0027 | 0.0067 | 0.0054 | 0.0007 | 0.0015 | 0.0007 | 0.0001 | 0.0009 | 0.001 | 0.0001 | 0.0003 | 0.0386 | 0.0001 | 0.0002 |
None | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0078 | 0.0001 | 0.0003 | 0.006 | 0 | 0.0001 | 0.0012 | 0 | 0 | 0.0003 | 0 | 0.0001 |
Nuclear Periphery | 0.0199 | 0.0085 | 0.0046 | 0.0032 | 0.0039 | 0.0048 | 0.0196 | 0.0045 | 0.0044 | 0.005 | 0.0022 | 0.0057 | 0.0232 | 0.0081 | 0.0057 | 0.007 | 0.005 | 0.0089 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0012 | 0.0009 | 0.0003 | 0.0001 | 0.0005 | 0.0003 | 0.0031 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0019 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0005 |
Peroxisomes | 0.0015 | 0.0001 | 0.0022 | 0.0001 | 0.0187 | 0.0005 | 0.0013 | 0 | 0.0065 | 0.0002 | 0 | 0 | 0.0006 | 0.0001 | 0 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0.005 | 0.0002 | 0.0006 | 0 | 0.0005 | 0.0001 | 0.0049 | 0 | 0.0004 | 0.0003 | 0 | 0 | 0.0015 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 |
Vacuole | 0.0025 | 0.0049 | 0.0023 | 0.0015 | 0.0046 | 0.0013 | 0.004 | 0.003 | 0.0021 | 0.004 | 0.0012 | 0.0015 | 0.0025 | 0.0018 | 0.0014 | 0.0013 | 0.0013 | 0.0012 |
Vacuole Periphery | 0.0016 | 0.0005 | 0.0004 | 0.0007 | 0.0008 | 0.0004 | 0.0016 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0.0007 | 0.0022 | 0.0002 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 123.715 | 98.0398 | 80.7345 | 90.2995 | 93.6401 | 129.6618 | 91.5725 | 97.5013 | 91.5682 | 102.8904 |
Translational Efficiency | 1.1616 | 1.0455 | 1.2335 | 1.0146 | 1.089 | 1.0265 | 1.0239 | 0.9637 | 1.0282 | 0.9818 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2188 | 1456 | 3019 | 781 | 2251 | 1633 | 2455 | 1121 | 4439 | 3089 | 5474 | 1902 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1398.15 | 1455.21 | 1969.03 | 1991.44 | 1485.74 | 1579.14 | 2049.62 | 2028.66 | 1442.57 | 1520.73 | 2005.17 | 2013.38 |
Standard Deviation | 233.94 | 319.61 | 279.99 | 336.85 | 253.39 | 277.27 | 304.44 | 335.89 | 247.90 | 304.33 | 293.95 | 336.78 |
Intensity Change Log 2 | — | 0.057708 | 0.493966 | 0.510293 | — | 0.087957 | 0.464175 | 0.449345 | — | 0.073371 | 0.478695 | 0.479215 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000362 | 0.001205 | 0.000131 | 0.002280 | 0.000260 | 0.001901 | 0.000910 | 0.001302 | 0.000310 | 0.001573 | 0.000481 | 0.001704 |
Bud Neck | 0.000081 | 0.000440 | 0.000111 | 0.000887 | 0.000114 | 0.000170 | 0.000566 | 0.000762 | 0.000098 | 0.000297 | 0.000315 | 0.000813 |
Bud Site | 0.001416 | 0.004709 | 0.000476 | 0.005953 | 0.001636 | 0.003348 | 0.002868 | 0.005488 | 0.001527 | 0.003989 | 0.001549 | 0.005679 |
Cell Periphery | 0.004192 | 0.004288 | 0.011009 | 0.036051 | 0.005634 | 0.005854 | 0.026606 | 0.030333 | 0.004923 | 0.005115 | 0.018004 | 0.032681 |
Cytoplasm | 0.001822 | 0.006866 | 0.002669 | 0.001466 | 0.001698 | 0.000743 | 0.003808 | 0.001847 | 0.001759 | 0.003629 | 0.003180 | 0.001690 |
Cytoplasmic Foci | 0.005000 | 0.015564 | 0.001047 | 0.001173 | 0.005258 | 0.002907 | 0.001895 | 0.002064 | 0.005131 | 0.008873 | 0.001427 | 0.001698 |
Eisosomes | 0.000102 | 0.000108 | 0.000082 | 0.000640 | 0.000109 | 0.000206 | 0.000451 | 0.000488 | 0.000105 | 0.000160 | 0.000248 | 0.000550 |
Endoplasmic Reticulum | 0.968653* | 0.888131* | 0.961371* | 0.892041* | 0.966545* | 0.966729* | 0.917242* | 0.906714* | 0.967584* | 0.929682* | 0.941580* | 0.900689* |
Endosome | 0.004977 | 0.018772 | 0.003064 | 0.006801 | 0.004662 | 0.004220 | 0.005542 | 0.006300 | 0.004817 | 0.011079 | 0.004175 | 0.006506 |
Golgi | 0.003190 | 0.018152 | 0.001829 | 0.014693 | 0.003727 | 0.004091 | 0.005689 | 0.011372 | 0.003463 | 0.010719 | 0.003560 | 0.012735 |
Lipid Particles | 0.003829 | 0.013870 | 0.009259 | 0.018187 | 0.004219 | 0.004382 | 0.018857 | 0.019883 | 0.004026 | 0.008854 | 0.013563 | 0.019187 |
Mitochondria | 0.000880 | 0.000769 | 0.000590 | 0.001616 | 0.001023 | 0.000955 | 0.001162 | 0.000491 | 0.000952 | 0.000867 | 0.000846 | 0.000953 |
Mitotic Spindle | 0.000060 | 0.000294 | 0.000072 | 0.001074 | 0.000055 | 0.000400 | 0.000429 | 0.000473 | 0.000057 | 0.000350 | 0.000232 | 0.000719 |
None | 0.001256 | 0.002283 | 0.001301 | 0.000364 | 0.000943 | 0.000443 | 0.000735 | 0.000419 | 0.001097 | 0.001310 | 0.001047 | 0.000397 |
Nuclear Periphery | 0.000145 | 0.000544 | 0.000463 | 0.002141 | 0.000162 | 0.000170 | 0.001325 | 0.001480 | 0.000154 | 0.000346 | 0.000849 | 0.001751 |
Nuclear Periphery Foci | 0.000735 | 0.009905 | 0.000965 | 0.002324 | 0.000351 | 0.000364 | 0.001179 | 0.001201 | 0.000540 | 0.004861 | 0.001061 | 0.001662 |
Nucleolus | 0.000004 | 0.000034 | 0.000008 | 0.000130 | 0.000007 | 0.000003 | 0.000018 | 0.000012 | 0.000006 | 0.000018 | 0.000012 | 0.000060 |
Nucleus | 0.000054 | 0.000191 | 0.000081 | 0.000348 | 0.000060 | 0.000039 | 0.000217 | 0.000134 | 0.000057 | 0.000111 | 0.000142 | 0.000222 |
Peroxisomes | 0.001275 | 0.000454 | 0.001756 | 0.000561 | 0.001398 | 0.000574 | 0.003861 | 0.000743 | 0.001337 | 0.000517 | 0.002700 | 0.000668 |
Vacuole | 0.000918 | 0.008587 | 0.003155 | 0.008205 | 0.001170 | 0.001448 | 0.005661 | 0.006349 | 0.001046 | 0.004813 | 0.004279 | 0.007111 |
Vacuole Periphery | 0.001049 | 0.004834 | 0.000560 | 0.003066 | 0.000970 | 0.001052 | 0.000982 | 0.002148 | 0.001009 | 0.002835 | 0.000749 | 0.002525 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.28 | 2.25 | -2.33 | -1.24 | -2.60 | -2.78 | -3.99 | -2.32 | 0.54 | -0.97 | -3.54 | -2.03 | -3.25 | -0.47 | -2.84 |
Bud Neck | -5.45 | -1.31 | -3.61 | -2.28 | -3.50 | -1.25 | -3.78 | -3.89 | -3.51 | -0.82 | -5.13 | -3.93 | -5.29 | -3.88 | -3.55 |
Bud Site | -4.92 | 2.93 | -3.23 | -1.14 | -3.86 | -1.93 | -2.00 | -2.88 | -1.48 | -1.89 | -4.38 | -0.17 | -4.30 | -1.82 | -4.24 |
Cell Periphery | -0.17 | -8.35 | -9.03 | -9.04 | -7.03 | -0.27 | -12.51 | -9.68 | -9.53 | -1.25 | -0.38 | -14.80 | -13.31 | -13.21 | -6.63 |
Cytoplasm | -6.23 | -3.03 | -0.27 | 3.86 | 0.60 | 3.92 | -4.20 | -0.51 | -2.17 | 2.56 | -4.63 | -5.21 | -0.52 | 2.54 | 2.08 |
Cytoplasmic Foci | -5.80 | 5.17 | 4.55 | 8.39 | -0.34 | 2.48 | 4.39 | 3.63 | 1.04 | -0.28 | -3.74 | 6.87 | 5.54 | 7.73 | -0.73 |
Eisosomes | -0.18 | 0.74 | -2.35 | -2.33 | -2.46 | -1.73 | -5.78 | -2.85 | -2.00 | -0.26 | -1.62 | -4.71 | -3.64 | -3.14 | -2.44 |
Endoplasmic Reticulum | 13.67 | 2.64 | 10.35 | -0.18 | 9.46 | -0.05 | 12.04 | 10.21 | 9.69 | 1.57 | 11.04 | 10.80 | 14.57 | 5.57 | 8.64 |
Endosome | -13.54 | 4.36 | -2.87 | 10.91 | -6.54 | 1.00 | -2.07 | -2.63 | -3.22 | -1.05 | -11.40 | 1.92 | -3.73 | 7.13 | -5.22 |
Golgi | -8.90 | 2.49 | -6.36 | 1.46 | -7.31 | -0.41 | -2.52 | -5.06 | -4.65 | -3.73 | -7.83 | -0.24 | -8.03 | -1.45 | -8.08 |
Lipid Particles | -7.15 | -5.40 | -6.53 | -1.77 | -3.85 | -0.21 | -9.56 | -7.86 | -7.56 | -0.35 | -6.23 | -10.81 | -10.23 | -6.41 | -3.34 |
Mitochondria | 0.41 | 1.21 | -1.13 | -1.34 | -1.65 | 0.16 | -0.50 | 1.76 | 1.26 | 3.70 | 0.32 | 0.51 | -0.06 | -0.31 | -0.43 |
Mitotic Spindle | -2.89 | -0.63 | -1.84 | -1.43 | -1.82 | -1.61 | -1.37 | -1.73 | -0.21 | -0.06 | -2.45 | -1.42 | -2.46 | -1.31 | -1.65 |
None | -1.39 | -0.14 | 3.17 | 2.68 | 3.81 | 1.98 | 1.05 | 2.16 | 0.08 | 1.47 | -0.58 | 0.25 | 3.69 | 2.47 | 3.74 |
Nuclear Periphery | -3.62 | -12.29 | -8.99 | -6.42 | -7.51 | -0.56 | -12.78 | -11.16 | -11.04 | -1.03 | -3.66 | -15.96 | -13.90 | -11.10 | -7.34 |
Nuclear Periphery Foci | -7.54 | -1.21 | -3.19 | 5.65 | -2.65 | -0.26 | -4.59 | -4.33 | -4.20 | 0.10 | -7.45 | -3.94 | -4.67 | 5.02 | -2.25 |
Nucleolus | -1.61 | -3.36 | -1.06 | -0.80 | -1.03 | 2.58 | -4.32 | -1.74 | -4.11 | 2.19 | -1.35 | -5.10 | -1.12 | -0.86 | -0.98 |
Nucleus | -2.95 | -3.09 | -1.71 | -0.98 | -1.56 | 2.43 | -5.56 | -2.94 | -3.70 | 2.01 | -2.40 | -6.25 | -2.27 | -1.56 | -1.18 |
Peroxisomes | 3.88 | -1.45 | 2.40 | -0.43 | 3.38 | 3.07 | -4.64 | 2.09 | -0.58 | 5.75 | 4.74 | -4.54 | 3.06 | -0.77 | 6.48 |
Vacuole | -7.96 | -5.45 | -6.39 | 0.02 | -4.35 | -0.53 | -7.23 | -6.28 | -5.58 | -0.49 | -7.06 | -8.92 | -9.03 | -2.93 | -3.87 |
Vacuole Periphery | -6.11 | 1.78 | -4.07 | 2.44 | -5.52 | -0.29 | -0.12 | -3.16 | -2.63 | -3.33 | -5.48 | 1.52 | -5.01 | 0.76 | -6.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|