Standard name
Description Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec66p, and Sec72p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.06 0.11 0.19 0.1 0.12 0.11 0.06 – 0.09 0.1 0.11 0.07 0.09 0 0.14 0.17 0.15 0.07 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0.09 0.09 0.19 0.08 0.08 0.08 0.12 – 0.12 0.17 0.18 0.12 0.2 0.26 0.49 0.53 0.39 0 0 0 0 0 0
Cytoplasm – 0.12 0.06 0 0.08 0.09 0.11 0.14 – 0.12 0.12 0.17 0.12 0.05 0.06 0.19 0.19 0.16 0 0 0 0 0 0
Endoplasmic Reticulum – 0.89 0.84 0.76 0.84 0.79 0.72 0.6 – 0.63 0.56 0.57 0.71 0.83 0.83 0 0 0 0.73 0.9 0.9 0.9 0.87 0.85
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0.19 0.08 0 0 0 – 0 0 0 0.09 0.05 0 0.22 0.2 0.44 0 0 0 0 0 0
Mitochondria – 0 0.1 0.15 0.05 0.15 0.28 0.3 – 0.19 0.4 0.34 0 0 0 0.08 0.08 0.06 0.05 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0.06 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0.06 0 0 0.06 0 – 0.11 0 0 0.14 0 0 0 0 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 12 16 10 18 28 35 15 – 34 23 24 26 47 21 23 21 19 2 5 5 6 16 13
Bud – 0 0 0 0 0 2 6 – 1 3 4 5 2 1 1 0 0 0 0 0 0 2 1
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 18 13 10 14 20 25 28 – 43 38 39 47 103 140 81 64 51 1 4 4 3 8 17
Cytoplasm – 25 8 2 15 22 33 34 – 43 28 37 47 25 35 31 23 21 1 3 1 3 6 5
Endoplasmic Reticulum – 184 118 41 157 191 220 145 – 231 127 125 268 421 448 1 5 0 30 191 123 258 390 404
Endosome – 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Golgi – 0 5 10 14 0 3 1 – 2 0 0 34 24 8 37 24 57 0 0 0 3 5 5
Mitochondria – 0 14 8 9 36 84 73 – 69 91 74 0 2 1 13 10 8 2 0 0 2 5 9
Nucleus – 0 0 0 1 1 1 1 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 5 3 2 1 9 5 – 6 6 10 0 1 1 1 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 2 1 3 8 4 17 9 – 41 8 6 54 11 5 4 2 27 0 2 0 3 6 10
Unique Cell Count 207 140 54 186 243 305 240 365 227 219 376 507 541 166 120 130 42 214 138 287 448 475
Labelled Cell Count 241 180 87 238 303 429 317 472 324 319 481 636 661 192 149 183 42 214 138 287 448 475


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 22.0 15.2 15.3 13.4 11.1 10.2 10.0 – 10.0 9.6 9.9 20.2 20.7 20.0 21.9 22.8 23.0 21.8 22.7 22.5
Std Deviation (1e-4) – 4.4 2.9 2.2 2.7 1.7 2.0 1.8 – 2.3 2.0 2.2 2.9 3.5 3.3 4.2 4.6 4.3 3.9 4.4 4.1
Intensity Change (Log2) – – – 0.0 -0.19 -0.46 -0.59 -0.61 – -0.62 -0.67 -0.63 0.41 0.44 0.39 0.52 0.58 0.59 0.52 0.57 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 -0.5 0 0 -1.8 – -0.7 -0.4 -0.1 -1.7 -0.8 -3.5 0.6 1.4 0.8
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 -0.6 -0.4 -0.4 0.7 – 0.8 2.0 2.2 1.0 3.0 4.2 7.5 7.8 5.8
Cytoplasm 0 0.8 1.2 1.7 2.5 – 2.0 2.1 3.1 2.2 -0.4 0.3 3.4 3.3 2.8
Endoplasmic Reticulum -1.4 0 -1.4 -2.8 -4.9 – -4.6 -5.6 -5.4 -3.0 -0.4 -0.4 -15.0 -12.9 -14.0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 1.5 0 0 0 – 0 0 0 2.1 0.6 0 4.7 4.2 7.9
Mitochondria 0.9 -1.8 1.3 4.1 4.6 – 2.4 6.2 5.1 0 0 0 -0.7 -0.5 -1.2
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0.5 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.4 0 – 3.8 0 0 4.5 0 0 0 0 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.9828 16.5588 16.5446 16.7797 15.892 16.0794 19.8902 20.6201 19.7866 18.6998 19.7706 19.6921 18.6704 16.7111 17.684 15.925 16.4171 16.837
Actin 0.0118 0.009 0.0217 0.0122 0.0074 0.0186 0.0369 0.0006 0.0104 0.001 0.0001 0.0046 0.0041 0.0018 0.0005 0.0218 0.0001 0.0006
Bud 0.0001 0.0003 0.0005 0 0.0006 0.0001 0.0005 0.0001 0.0001 0.0003 0 0.0003 0.0001 0.0001 0 0.0002 0 0
Bud Neck 0.0003 0.0007 0.0025 0.001 0.0083 0.0007 0.0006 0.0004 0.0007 0.0002 0.0002 0.001 0.0002 0.0012 0.0002 0.0005 0.0001 0.0017
Bud Periphery 0.0002 0.001 0.0007 0.0001 0.0006 0.0002 0.0012 0.0005 0.0003 0.0012 0.0001 0.0007 0.0002 0.0002 0.0001 0.0005 0 0.0001
Bud Site 0.0004 0.0029 0.0037 0 0.0136 0.0001 0.0013 0.0007 0.0053 0.0002 0 0.0002 0.0002 0.0059 0 0.0004 0 0
Cell Periphery 0.0022 0.022 0.0066 0.0047 0.0025 0.0009 0.0064 0.0169 0.0079 0.005 0.002 0.0016 0.0039 0.0099 0.0029 0.0016 0.0032 0.0015
Cytoplasm 0.019 0.0351 0.017 0.0028 0.0423 0.0061 0.0078 0.0095 0.0052 0.0111 0.0017 0.0019 0.0052 0.0347 0.0082 0.0143 0.0022 0.0025
Cytoplasmic Foci 0.0069 0.0021 0.0076 0.0023 0.0276 0.0051 0.0054 0.0004 0.0046 0.0036 0.0001 0.0002 0.0031 0.0019 0.0002 0.0003 0.0001 0.0001
Eisosomes 0.0006 0.0009 0.0002 0.0004 0.0001 0.0001 0.0011 0.0003 0.0013 0.0036 0 0.0001 0.0005 0.0001 0 0.0012 0.0001 0.0001
Endoplasmic Reticulum 0.8923 0.8864 0.9023 0.9236 0.8287 0.9225 0.85 0.9487 0.9211 0.9476 0.9903 0.9738 0.9386 0.9232 0.977 0.8992 0.9834 0.979
Endosome 0.0165 0.0063 0.0059 0.0076 0.0147 0.0102 0.0151 0.0018 0.0086 0.005 0.0005 0.0027 0.0044 0.0011 0.0009 0.0038 0.001 0.0016
Golgi 0.0087 0.01 0.0069 0.0371 0.0138 0.0166 0.0147 0.0029 0.0076 0.0017 0.0007 0.003 0.003 0.0021 0.0011 0.0046 0.0021 0.0007
Lipid Particles 0.0067 0.0076 0.0079 0.0018 0.0076 0.0045 0.0103 0.0082 0.0109 0.0022 0.0006 0.0008 0.0035 0.0068 0.0005 0.0013 0.0009 0.0005
Mitochondria 0.0013 0.0005 0.0058 0.0008 0.0027 0.0067 0.0054 0.0007 0.0015 0.0007 0.0001 0.0009 0.001 0.0001 0.0003 0.0386 0.0001 0.0002
None 0.0011 0.0001 0.0002 0.0001 0.0002 0.0001 0.0078 0.0001 0.0003 0.006 0 0.0001 0.0012 0 0 0.0003 0 0.0001
Nuclear Periphery 0.0199 0.0085 0.0046 0.0032 0.0039 0.0048 0.0196 0.0045 0.0044 0.005 0.0022 0.0057 0.0232 0.0081 0.0057 0.007 0.005 0.0089
Nucleolus 0.0002 0 0.0001 0 0.0002 0 0.0008 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0
Nucleus 0.0012 0.0009 0.0003 0.0001 0.0005 0.0003 0.0031 0.0003 0.0002 0.0004 0.0001 0.0003 0.0019 0.0006 0.0002 0.0005 0.0001 0.0005
Peroxisomes 0.0015 0.0001 0.0022 0.0001 0.0187 0.0005 0.0013 0 0.0065 0.0002 0 0 0.0006 0.0001 0 0.0003 0 0
Punctate Nuclear 0.005 0.0002 0.0006 0 0.0005 0.0001 0.0049 0 0.0004 0.0003 0 0 0.0015 0.0001 0 0.0002 0 0.0002
Vacuole 0.0025 0.0049 0.0023 0.0015 0.0046 0.0013 0.004 0.003 0.0021 0.004 0.0012 0.0015 0.0025 0.0018 0.0014 0.0013 0.0013 0.0012
Vacuole Periphery 0.0016 0.0005 0.0004 0.0007 0.0008 0.0004 0.0016 0.0005 0.0005 0.0006 0.0002 0.0007 0.0009 0.0002 0.0007 0.0022 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 123.715 98.0398 80.7345 90.2995 93.6401 129.6618 91.5725 97.5013 91.5682 102.8904
Translational Efficiency 1.1616 1.0455 1.2335 1.0146 1.089 1.0265 1.0239 0.9637 1.0282 0.9818

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘