Standard name
Description Sorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.08 0.09 0.1 0.09 0.09 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.18 0.08 0.12 0.12 0.12 0.13 0.13 0.09 0.18 0.16 0.15 0.13 0.14 0.14 0.13 0.16 0.11 0.05 0 0 0.05 0.05 0.05
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.6 0.43 0.82 0.63 0.66 0.55 0.43 0.53 0.42 0.27 0.22 0.27 0.68 0.72 0.7 0.61 0.58 0.7 0.7 0.79 0.8 0.67 0.76 0.72
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.12 0 0.16 0.1 0.21 0.28 0.2 0.31 0.41 0.53 0.36 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0 0 0.07 0 0.06 0.07 0.06 0.06 0 0 0 0.09 0.07 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.23 0.15 0.16 0.09 0.13 0.08 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.13 0 0.25 0.28 0.26 0.24 0.25 0.29 0.23 0.15 0.13 0.24 0.26 0.28 0.25 0.28 0.26 0.28 0.08 0 0.07 0.1 0.07 0.06
Vac/Vac Membrane 0.13 0.24 0.08 0 0.05 0 0 0 0 0.05 0.05 0 0.12 0.18 0.18 0.16 0.17 0.12 0 0.06 0 0.07 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 1 2 3 1 10 4 4 6 1 8 18 4 14 7 6 8 5 2 2 1 1 2
Bud 8 5 5 6 12 22 28 32 28 30 38 41 8 3 4 4 3 2 1 3 1 2 1 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 1 3 0 4
Bud Site 1 0 0 1 0 1 3 1 2 2 3 5 0 0 0 0 0 1
Cell Periphery 0 0 0 1 0 0 0 1 1 5 4 6 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 46 27 16 19 31 33 42 42 28 57 49 55 32 67 66 30 26 17 19 5 2 16 20 19
Endoplasmic Reticulum 4 12 4 1 2 7 5 2 3 8 2 4 6 0 6 3 9 6 5 1 2 2 2 1
Endosome 193 65 173 103 171 155 139 173 124 85 68 97 166 349 335 137 93 103 252 143 181 218 313 297
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 8 4 2 7 1 4 1 3 3
Mitochondria 69 18 3 26 26 60 93 65 93 130 164 127 2 1 0 11 9 3 15 2 4 11 15 11
Nucleus 1 0 1 0 1 0 0 1 1 0 0 0 1 1 0 0 0 0 0 0 0 1 1 1
Nuclear Periphery 1 1 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 1 1 1 2 1 3
Nucleolus 8 6 5 8 10 11 23 12 18 22 19 22 11 15 12 20 12 9 0 0 0 2 2 2
Peroxisomes 10 0 15 37 40 46 31 44 23 14 9 26 0 7 12 1 1 2 3 0 2 0 0 0
SpindlePole 41 6 53 46 68 69 83 95 69 48 39 86 63 136 119 62 41 41 29 4 16 31 27 25
Vac/Vac Membrane 41 37 16 2 14 8 6 6 10 15 14 12 29 85 87 35 27 18 12 11 2 23 16 28
Unique Cell Count 321 152 211 164 259 282 327 327 296 318 308 356 245 484 482 225 160 148 362 182 226 325 411 412
Labelled Cell Count 427 180 292 252 378 413 463 479 405 423 410 490 336 669 656 318 231 212 362 182 226 325 411 412


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.8 7.0 6.2 7.0 6.3 6.1 6.3 6.0 5.7 5.4 5.7 7.9 8.7 8.5 10.7 9.9 10.4 7.8 8.0 8.6
Std Deviation (1e-4) 0.7 1.2 1.2 1.1 1.7 1.0 1.1 1.4 1.4 1.5 1.3 1.2 1.1 1.0 1.0 1.9 1.8 1.8 1.0 1.1 1.3
Intensity Change (Log2) -0.17 0.0 -0.15 -0.19 -0.15 -0.2 -0.28 -0.35 -0.29 0.19 0.32 0.29 0.62 0.51 0.58 0.16 0.2 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0
Bud 0 1.3 2.6 2.9 3.3 3.2 3.2 4.0 3.9 0.6 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.6 1.5 1.9 1.9 0.7 3.4 2.8 2.7 1.9 2.3 2.3 2.0 2.6 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.9 0
Endosome -4.2 -3.9 -6.3 -9.1 -6.9 -9.0 -12.5 -13.4 -12.6 -3.5 -2.8 -3.4 -4.9 -5.1 -2.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.2 3.9 6.5 8.0 6.3 8.5 10.2 12.4 9.4 0 0 0 2.1 2.3 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.3 0.9 0.9 2.4 0.8 2.0 2.3 2.0 2.1 1.2 0.5 0.1 2.9 2.3 1.8
Peroxisomes 4.3 2.8 3.1 1.0 2.3 0.3 -1.3 -2.2 0.1 -4.2 -3.9 -2.9 -3.7 -3.0 -2.5
SpindlePole 0.6 0.3 -0.2 0.1 1.0 -0.5 -2.9 -3.6 -0.3 0.1 0.8 -0.1 0.6 0.1 0.5
Vacuole -2.9 -1.0 -2.4 -3.3 -3.3 -2.1 -1.4 -1.5 -2.2 1.5 3.4 3.6 2.6 2.8 1.5