Standard name | |
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Description | Sorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 2 | 1 | 2 | 3 | 1 | 10 | 4 | 4 | 6 | 1 | 8 | 18 | 4 | 14 | 7 | 6 | 8 | 5 | 2 | 2 | 1 | 1 | 2 |
Bud | 8 | 5 | 5 | 6 | 12 | 22 | 28 | 32 | 28 | 30 | 38 | 41 | 8 | 3 | 4 | 4 | 3 | 2 | 1 | 3 | 1 | 2 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 0 | 4 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 2 | 2 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 46 | 27 | 16 | 19 | 31 | 33 | 42 | 42 | 28 | 57 | 49 | 55 | 32 | 67 | 66 | 30 | 26 | 17 | 19 | 5 | 2 | 16 | 20 | 19 |
Endoplasmic Reticulum | 4 | 12 | 4 | 1 | 2 | 7 | 5 | 2 | 3 | 8 | 2 | 4 | 6 | 0 | 6 | 3 | 9 | 6 | 5 | 1 | 2 | 2 | 2 | 1 |
Endosome | 193 | 65 | 173 | 103 | 171 | 155 | 139 | 173 | 124 | 85 | 68 | 97 | 166 | 349 | 335 | 137 | 93 | 103 | 252 | 143 | 181 | 218 | 313 | 297 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 8 | 4 | 2 | 7 | 1 | 4 | 1 | 3 | 3 |
Mitochondria | 69 | 18 | 3 | 26 | 26 | 60 | 93 | 65 | 93 | 130 | 164 | 127 | 2 | 1 | 0 | 11 | 9 | 3 | 15 | 2 | 4 | 11 | 15 | 11 |
Nucleus | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 3 |
Nucleolus | 8 | 6 | 5 | 8 | 10 | 11 | 23 | 12 | 18 | 22 | 19 | 22 | 11 | 15 | 12 | 20 | 12 | 9 | 0 | 0 | 0 | 2 | 2 | 2 |
Peroxisomes | 10 | 0 | 15 | 37 | 40 | 46 | 31 | 44 | 23 | 14 | 9 | 26 | 0 | 7 | 12 | 1 | 1 | 2 | 3 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 41 | 6 | 53 | 46 | 68 | 69 | 83 | 95 | 69 | 48 | 39 | 86 | 63 | 136 | 119 | 62 | 41 | 41 | 29 | 4 | 16 | 31 | 27 | 25 |
Vac/Vac Membrane | 41 | 37 | 16 | 2 | 14 | 8 | 6 | 6 | 10 | 15 | 14 | 12 | 29 | 85 | 87 | 35 | 27 | 18 | 12 | 11 | 2 | 23 | 16 | 28 |
Unique Cell Count | 321 | 152 | 211 | 164 | 259 | 282 | 327 | 327 | 296 | 318 | 308 | 356 | 245 | 484 | 482 | 225 | 160 | 148 | 362 | 182 | 226 | 325 | 411 | 412 |
Labelled Cell Count | 427 | 180 | 292 | 252 | 378 | 413 | 463 | 479 | 405 | 423 | 410 | 490 | 336 | 669 | 656 | 318 | 231 | 212 | 362 | 182 | 226 | 325 | 411 | 412 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 6.8 | 7.0 | 6.2 | 7.0 | 6.3 | 6.1 | 6.3 | 6.0 | 5.7 | 5.4 | 5.7 | 7.9 | 8.7 | 8.5 | 10.7 | 9.9 | 10.4 | 7.8 | 8.0 | 8.6 |
Std Deviation (1e-4) | 0.7 | 1.2 | 1.2 | 1.1 | 1.7 | 1.0 | 1.1 | 1.4 | 1.4 | 1.5 | 1.3 | 1.2 | 1.1 | 1.0 | 1.0 | 1.9 | 1.8 | 1.8 | 1.0 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.0 | -0.15 | -0.19 | -0.15 | -0.2 | -0.28 | -0.35 | -0.29 | 0.19 | 0.32 | 0.29 | 0.62 | 0.51 | 0.58 | 0.16 | 0.2 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.3 | 2.6 | 2.9 | 3.3 | 3.2 | 3.2 | 4.0 | 3.9 | 0.6 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 1.6 | 1.5 | 1.9 | 1.9 | 0.7 | 3.4 | 2.8 | 2.7 | 1.9 | 2.3 | 2.3 | 2.0 | 2.6 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 |
Endosome | -4.2 | -3.9 | -6.3 | -9.1 | -6.9 | -9.0 | -12.5 | -13.4 | -12.6 | -3.5 | -2.8 | -3.4 | -4.9 | -5.1 | -2.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.2 | 3.9 | 6.5 | 8.0 | 6.3 | 8.5 | 10.2 | 12.4 | 9.4 | 0 | 0 | 0 | 2.1 | 2.3 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.3 | 0.9 | 0.9 | 2.4 | 0.8 | 2.0 | 2.3 | 2.0 | 2.1 | 1.2 | 0.5 | 0.1 | 2.9 | 2.3 | 1.8 |
Peroxisomes | 4.3 | 2.8 | 3.1 | 1.0 | 2.3 | 0.3 | -1.3 | -2.2 | 0.1 | -4.2 | -3.9 | -2.9 | -3.7 | -3.0 | -2.5 |
SpindlePole | 0.6 | 0.3 | -0.2 | 0.1 | 1.0 | -0.5 | -2.9 | -3.6 | -0.3 | 0.1 | 0.8 | -0.1 | 0.6 | 0.1 | 0.5 |
Vacuole | -2.9 | -1.0 | -2.4 | -3.3 | -3.3 | -2.1 | -1.4 | -1.5 | -2.2 | 1.5 | 3.4 | 3.6 | 2.6 | 2.8 | 1.5 |