Standard name
Description Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin; human homolog ELP3 can partially complement yeast elp3 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.07 0.05 0 0 0 0 0.06 0 0 0 0 0 0.17 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.96 0.95 0.95 0.89 0.83 0.84 0.78 0.85 1.0 0.98 0.99 0.91 0.97 0.91 0.94 0.97 0.94 0.94 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.14 0.07 0.26 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 2 13 7 8 12 5 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 2 4 5 3 9 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 14 18 17 15 31 20 27 16 16 0 0 3 27 10 14 0 0 0 0 0 0
Cytoplasm 286 88 201 360 452 452 638 447 366 391 421 17 60 140 146 169 144 267 89 203 18 35 43
Endoplasmic Reticulum 1 3 6 7 14 16 29 7 32 13 21 0 0 1 8 8 7 4 0 3 0 0 1
Endosome 1 0 0 1 1 0 4 2 0 4 2 0 0 0 0 0 0 2 0 0 0 0 0
Golgi 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 4 0 2 0 0 0
Mitochondria 4 0 0 1 3 12 35 77 31 131 67 0 0 0 0 2 0 2 0 1 0 0 0
Nucleus 0 1 1 2 3 0 5 0 2 0 3 0 1 1 4 1 1 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 4 9 2 12 20 4 12 5 0 0 0 4 3 11 1 1 2 0 3 4
Unique Cell Count 293 89 208 375 474 478 717 537 438 503 498 17 61 142 160 175 158 286 93 217 20 43 52
Labelled Cell Count 296 96 224 394 499 501 774 586 474 589 545 17 61 145 189 193 178 286 93 217 20 43 52


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 13.4 11.3 11.0 10.5 8.4 7.8 6.9 7.1 6.5 7.0 9.1 10.6 10.4 17.7 16.4 16.6
Std Deviation (1e-4) 1.4 2.0 1.3 1.3 1.3 1.0 1.3 1.2 1.0 1.2 1.1 0.8 2.0 1.5 3.2 2.7 3.0
Intensity Change (Log2) -0.04 -0.11 -0.43 -0.54 -0.7 -0.66 -0.78 -0.69 -0.3 -0.1 -0.12 0.65 0.54 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -1.8 -2.1 -1.4 -1.8 -0.3 -2.1 -2.1 0 0 -2.0 3.1 -0.4 0.8
Cytoplasm -0.4 -0.8 -1.2 -3.3 -4.9 -4.7 -6.1 -4.5 0.8 0.7 1.1 -2.2 0 -2.2
Endoplasmic Reticulum 0 0 0.3 0.8 0 2.2 -0.2 0.8 0 0 0 1.1 0.9 0.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.2 5.8 3.9 8.1 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.8 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available