Standard name
Human Ortholog
Description COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.57 0.4 0.6 0.54 0.48 0.43 0.25 0.19 0.2 0.15 0.11 0.14 0.51 0.5 0.45 0.45 0.41 0.45 0.46 0.29 0.49 0.5 0.6 0.52
Bud 0 0 0.13 0.13 0.15 0.09 0.15 0.09 0.08 0.08 0.11 0.09 0.05 0 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.08 0.12 0.1 0.08 0 0 0 0.05 0 0.05 0.08 0 0.06 0.09 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0 0.13 0.07 0.07 0.05 0.06 0.07 0 0 0.05 0 0 0.09 0.09 0.06 0.1 0.14 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.45 0.75 0.41 0.3 0.3 0.39 0.2 0.2 0.22 0.17 0.2 0.16 0.64 0.52 0.45 0.09 0.07 0 0.28 0.38 0.18 0.26 0.21 0.27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.62 0.06 0.09 0 0.06 0 0 0 0 0 0 0 0.25 0.31 0.16 0.74 0.67 0.67 0.19 0.18 0.18 0.1 0.11 0.12
Mitochondria 0.06 0.07 0.26 0.62 0.42 0.59 0.72 0.76 0.83 0.83 0.85 0.87 0 0.11 0.34 0.05 0.08 0.08 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.12 0 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 189 111 285 199 210 261 173 147 123 100 56 97 99 126 117 54 43 53 171 85 270 73 129 100
Bud 11 10 63 47 66 53 101 71 48 54 56 60 9 11 22 0 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 1 1 7 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 13 40 44 42 49 31 21 21 36 22 37 16 9 17 11 10 14 2 2 13 2 5 7
Cytoplasm 9 37 31 24 23 35 48 29 27 34 19 21 18 23 17 12 15 20 2 6 2 3 0 0
Endoplasmic Reticulum 149 210 195 109 130 236 139 153 136 110 98 111 124 132 117 11 7 4 104 112 97 39 44 52
Endosome 9 3 15 0 16 5 23 21 2 2 0 2 1 1 3 3 1 0 6 6 8 1 1 1
Golgi 207 18 41 9 25 5 6 1 3 2 3 3 49 78 43 88 71 79 71 51 97 15 22 23
Mitochondria 21 19 126 227 183 358 496 576 502 546 415 611 5 28 88 6 9 10 4 12 28 6 4 2
Nucleus 0 0 0 0 0 1 0 0 3 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 3 0 2 1 2 3 2 1 0 4 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 26 4 52 16 40 41 12 38 18 24 3 5 5 1 0 1 3 1 17 0 1 0
SpindlePole 0 0 0 0 4 0 4 3 1 6 7 2 0 1 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 4 1 2 0 2 1 3 18 0 2 1 2 0 1 1 1 3 2 0 5 2 2 0 0
Unique Cell Count 333 281 476 367 433 603 691 762 607 656 490 702 194 254 262 119 106 118 369 293 546 149 214 193
Labelled Cell Count 614 423 824 663 753 1024 1065 1085 880 941 701 975 328 415 434 188 160 185 369 293 546 149 214 193


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 10.3 7.6 6.8 7.2 6.3 6.0 5.9 5.3 5.2 4.9 4.8 10.6 10.4 9.3 12.1 14.0 13.0 10.9 12.1 12.4
Std Deviation (1e-4) 1.9 1.7 1.0 1.1 1.0 0.9 0.9 1.0 1.0 1.0 0.9 0.9 1.7 1.9 1.6 3.0 2.5 2.6 2.2 2.0 2.1
Intensity Change (Log2) – – – -0.15 -0.07 -0.26 -0.33 -0.35 -0.52 -0.54 -0.62 -0.65 0.48 0.46 0.31 0.68 0.89 0.79 0.52 0.68 0.71

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -3.4 -5.4 -12.0 -14.6 -13.4 -15.6 -15.8 0 -2.1 -2.7 -4.0 -2.9 -3.6 -2.9
Bud -0.2 0.9 -2.3 0.7 -2.2 -2.9 -2.7 -0.9 0 -3.3 -3.8 -2.0 -4.2 -3.7 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 0.7 -0.2 -2.8 -4.5 -3.5 -1.9 -2.5 0 -0.1 -2.5 -0.9 0.3 0.3 1.2
Cytoplasm 0 -0.8 -0.5 0.3 -2.2 -1.5 -0.9 -1.8 -2.9 1.2 1.3 0 1.3 2.6 3.6
Endoplasmic Reticulum -3.4 -3.4 -0.6 -7.7 -8.0 -6.6 -9.1 -7.1 0 5.4 2.8 1.0 -6.5 -6.7 -7.7
Endosome -3.4 0.5 -2.8 0.2 -0.4 -3.7 -3.9 -4.0 0 0 -2.4 -1.7 0 0 0
Golgi -3.8 -1.6 -6.3 -6.6 -8.0 -6.7 -7.2 -6.0 0 5.7 7.7 3.2 15.5 13.8 14.1
Mitochondria 10.3 5.0 10.8 15.2 17.0 18.6 19.2 18.2 0 -7.1 -4.9 2.0 -5.0 -4.0 -4.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.4 3.5 -2.4 0.2 -0.1 -3.1 0.2 -1.3 0 -2.3 -2.2 -2.3 -2.2 0 -2.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5791 8.7948 7.9992 6.842 7.6838 8.1845 8.8559 11.4853 10.374 9.0988 9.5807 10.4539 10.8078 11.8212 11.0547 10.8076 11.0423 11.2234
Actin 0.2528 0.2018 0.1973 0.2067 0.0788 0.3162 0.202 0.2873 0.235 0.2415 0.169 0.2004 0.1431 0.1901 0.1943 0.1294 0.3093 0.2294
Bud 0.0006 0.0003 0.0006 0.0007 0.0024 0.0003 0.0005 0.0029 0.0005 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003
Bud Neck 0.0081 0.0019 0.0021 0.0029 0.0012 0.0076 0.0084 0.0021 0.0015 0.0011 0.0014 0.0033 0.001 0.0014 0.0015 0.0021 0.0039 0.0031
Bud Periphery 0.0008 0.0003 0.0015 0.0011 0.0024 0.0004 0.0005 0.0007 0.0004 0.0003 0.0002 0.0003 0.0004 0.0002 0.0002 0.0001 0.0007 0.0005
Bud Site 0.0134 0.0033 0.0035 0.0015 0.0052 0.0006 0.0107 0.0186 0.0055 0.0004 0.0003 0.0002 0.0012 0.0007 0.0004 0.0002 0.0007 0.0033
Cell Periphery 0.0013 0.002 0.0018 0.0033 0.0006 0.0013 0.0011 0.001 0.0009 0.0015 0.0006 0.0013 0.0008 0.0012 0.0014 0.0027 0.0021 0.0012
Cytoplasm 0.0234 0.024 0.0147 0.004 0.0027 0.0065 0.0085 0.0269 0.0128 0.0047 0.006 0.007 0.0032 0.0097 0.0079 0.0067 0.0037 0.0113
Cytoplasmic Foci 0.029 0.0431 0.0311 0.0296 0.0156 0.0232 0.0392 0.0465 0.034 0.0294 0.026 0.0263 0.0251 0.025 0.0159 0.0257 0.018 0.0178
Eisosomes 0.0025 0.0033 0.0013 0.0036 0.0004 0.002 0.0022 0.0009 0.002 0.0024 0.0011 0.0047 0.003 0.0036 0.0113 0.0068 0.0169 0.0064
Endoplasmic Reticulum 0.0268 0.0329 0.0287 0.0138 0.0145 0.0144 0.0162 0.0244 0.0078 0.0141 0.0348 0.0355 0.005 0.0114 0.008 0.0432 0.0073 0.0199
Endosome 0.0576 0.0546 0.0508 0.0867 0.039 0.077 0.05 0.047 0.0308 0.0376 0.0686 0.0461 0.0402 0.0274 0.0231 0.0273 0.0228 0.0281
Golgi 0.4917 0.5802 0.6098 0.5491 0.7522 0.5105 0.5754 0.5212 0.6394 0.5947 0.662 0.6432 0.7333 0.7177 0.7191 0.7183 0.6053 0.6621
Lipid Particles 0.0564 0.0477 0.0391 0.0265 0.0667 0.0323 0.0189 0.0164 0.0147 0.0595 0.0195 0.0184 0.0203 0.0084 0.0132 0.0122 0.0043 0.0068
Mitochondria 0.0098 0.0013 0.0074 0.0623 0.0136 0.0022 0.0066 0.0014 0.0024 0.0052 0.0055 0.0077 0.0092 0.0015 0.0015 0.0041 0.0035 0.007
None 0.0046 0.0002 0.001 0.0004 0.0002 0.0001 0.0054 0.0002 0.0002 0.0003 0.0002 0.0003 0.0021 0.0001 0.0001 0.0002 0.0002 0.0004
Nuclear Periphery 0.0032 0.0006 0.0013 0.0012 0.0009 0.0005 0.0058 0.0003 0.0002 0.0003 0.0003 0.0007 0.001 0.0002 0.0002 0.0001 0.0002 0.0003
Nucleolus 0.0006 0 0.0001 0.0001 0.0001 0 0.0166 0 0 0 0 0 0.0004 0 0 0 0 0
Nucleus 0.0055 0.0001 0.0003 0.0002 0.0004 0.0001 0.0146 0.0001 0 0 0.0001 0.0001 0.0003 0 0 0 0 0.0001
Peroxisomes 0.0064 0.001 0.005 0.0026 0.0013 0.0027 0.0042 0.0011 0.0112 0.0057 0.0018 0.0034 0.0071 0.0006 0.0011 0.0202 0.0006 0.0011
Punctate Nuclear 0.0027 0.0002 0.0004 0.0005 0.0003 0.0002 0.0111 0.0002 0.0003 0.0003 0.0001 0.0002 0.001 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0015 0.0006 0.0014 0.0016 0.0007 0.0009 0.0011 0.0004 0.0002 0.0004 0.0012 0.0004 0.0013 0.0002 0.0002 0.0002 0.0002 0.0003
Vacuole Periphery 0.0013 0.0005 0.0008 0.0018 0.0009 0.0009 0.0011 0.0003 0.0002 0.0004 0.0014 0.0006 0.0008 0.0002 0.0003 0.0002 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2412 47.0131 55.3283 58.6992 45.6024 45.0291 57.0721 58.6993 48.9609 55.2699
Translational Efficiency 1.8038 1.7512 1.3619 1.1771 1.4576 1.6381 1.1259 1.1045 1.2582 1.1494

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1232 1508 1936 1236 1597 1664 953 1611 2829 3172 2889 2847

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 879.00 1151.66 1623.48 1372.39 966.92 1095.05 1321.06 1441.97 928.63 1121.96 1523.72 1411.76
Standard Deviation 157.91 190.25 236.14 214.49 182.30 163.84 181.38 212.14 177.54 179.13 261.60 215.93
Intensity Change Log 2 — 0.389780 0.885155 0.642755 — 0.179528 0.450228 0.576573 — 0.283474 0.673702 0.608467

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.268164 0.118694 0.043586 0.079825 0.101854 0.127762 0.105396 0.050790 0.174280 0.123451 0.063975 0.063395
Bud Neck 0.000769 0.000844 0.000959 0.000896 0.000478 0.000569 0.000987 0.001398 0.000605 0.000700 0.000968 0.001180
Bud Site 0.002629 0.002437 0.004838 0.004799 0.001557 0.002424 0.005049 0.003861 0.002024 0.002431 0.004908 0.004268
Cell Periphery 0.005772 0.008599 0.009410 0.011139 0.013243 0.011146 0.005695 0.032810 0.009989 0.009935 0.008185 0.023402
Cytoplasm 0.006087 0.001602 0.001711 0.002238 0.002545 0.002267 0.002009 0.001590 0.004087 0.001951 0.001810 0.001872
Cytoplasmic Foci 0.022932 0.029173 0.048945 0.059626 0.017409 0.025855 0.030599 0.021342 0.019814 0.027433 0.042893 0.037963
Eisosomes 0.042099 0.071457 0.009583 0.023768 0.095941 0.097362 0.014227 0.080100 0.072493 0.085046 0.011115 0.055644
Endoplasmic Reticulum 0.129662 0.187462 0.386252 0.222455 0.153002 0.160791 0.323312 0.316549 0.142837 0.173471 0.365490 0.275699
Endosome 0.072962 0.068337 0.059408 0.079795 0.051530 0.049793 0.110163 0.048340 0.060864 0.058609 0.076151 0.061996
Golgi 0.278606 0.282687 0.202374 0.165768 0.290507 0.292737 0.124900 0.135194 0.285324 0.287959 0.176818 0.148468
Lipid Particles 0.132416 0.191562 0.214524 0.320518 0.180272 0.162453 0.264514 0.264965 0.159432 0.176292 0.231014 0.289083
Mitochondria 0.004022 0.000475 0.000149 0.000413 0.001743 0.000483 0.000072 0.000360 0.002736 0.000480 0.000124 0.000383
Mitotic Spindle 0.000084 0.000705 0.000078 0.002101 0.000223 0.000285 0.000083 0.000464 0.000163 0.000485 0.000079 0.001175
None 0.001430 0.000337 0.000236 0.000110 0.001165 0.001309 0.000042 0.000236 0.001281 0.000847 0.000172 0.000181
Nuclear Periphery 0.000061 0.000146 0.000126 0.000109 0.000083 0.000071 0.000053 0.000439 0.000073 0.000106 0.000102 0.000296
Nuclear Periphery Foci 0.000155 0.000731 0.000812 0.000635 0.000213 0.000106 0.000860 0.000390 0.000188 0.000403 0.000828 0.000496
Nucleolus 0.000234 0.000038 0.000002 0.000002 0.000021 0.000005 0.000001 0.000002 0.000114 0.000020 0.000001 0.000002
Nucleus 0.002785 0.000211 0.000083 0.000071 0.000263 0.000172 0.000047 0.000126 0.001361 0.000191 0.000071 0.000102
Peroxisomes 0.027052 0.032611 0.010974 0.020656 0.086573 0.063640 0.009713 0.038834 0.060652 0.048888 0.010558 0.030942
Vacuole 0.001152 0.000722 0.004438 0.003283 0.000431 0.000336 0.001989 0.001420 0.000745 0.000519 0.003630 0.002229
Vacuole Periphery 0.000926 0.001171 0.001512 0.001794 0.000947 0.000432 0.000289 0.000789 0.000938 0.000783 0.001108 0.001225

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 15.87 26.65 20.55 5.90 -7.15 -3.90 -0.48 9.32 13.17 8.43 8.84 20.98 20.98 13.77 0.15
Bud Neck -0.33 -1.96 -1.44 -0.22 0.68 -1.82 -5.14 -7.31 -6.34 -2.64 -0.88 -5.54 -7.12 -3.75 -2.25
Bud Site 0.35 -6.19 -5.32 -3.94 0.03 -1.72 -3.87 -4.33 -2.05 1.13 -1.14 -7.72 -6.42 -3.88 1.28
Cell Periphery -4.26 -7.56 -6.51 -2.64 -2.05 1.89 7.29 -11.17 -13.97 -18.09 0.08 2.86 -12.48 -13.57 -16.02
Cytoplasm 2.98 2.92 2.56 -2.22 -2.08 0.63 1.30 1.37 1.09 0.68 3.05 3.29 2.90 0.21 -0.20
Cytoplasmic Foci -2.67 -9.67 -10.91 -8.99 -2.92 -4.22 -5.68 -2.12 1.99 3.60 -5.05 -12.43 -9.87 -5.29 2.17
Eisosomes -6.36 11.92 5.59 11.17 -6.82 -0.26 19.34 2.98 3.25 -16.62 -3.37 23.32 4.95 8.27 -18.66
Endoplasmic Reticulum -7.48 -30.60 -10.46 -3.83 16.96 -1.06 -16.26 -18.67 -17.73 0.59 -5.72 -34.24 -20.96 -15.94 12.15
Endosome 1.37 4.54 -1.91 -3.57 -7.24 0.70 -13.79 1.32 0.73 15.56 1.09 -6.76 -0.56 -1.86 6.82
Golgi -0.49 9.50 13.47 15.83 5.50 -0.27 23.33 21.98 22.86 -1.92 -0.45 19.62 25.29 27.80 6.33
Lipid Particles -7.81 -11.37 -19.83 -13.57 -11.49 2.68 -9.05 -10.95 -13.89 -0.05 -3.34 -12.84 -21.44 -19.07 -9.12
Mitochondria 4.58 5.05 4.61 0.34 -1.64 2.35 3.13 2.54 0.91 -2.38 4.97 5.80 5.12 0.86 -2.67
Mitotic Spindle -1.35 0.37 -2.36 -1.43 -2.37 -0.38 1.58 -0.82 -0.56 -1.34 -1.36 1.65 -2.49 -1.47 -2.71
None 2.39 2.60 2.88 6.15 3.73 -0.27 5.15 4.25 2.29 -7.61 1.29 4.71 4.68 2.69 -0.34
Nuclear Periphery -1.28 -11.77 -3.76 0.54 1.28 1.75 4.18 -13.93 -14.80 -15.48 -1.04 -5.66 -14.08 -5.39 -12.36
Nuclear Periphery Foci -1.08 -10.28 -4.94 0.17 1.66 1.61 -6.73 -1.40 -2.57 3.56 -0.84 -10.91 -3.69 -0.36 3.84
Nucleolus 1.08 1.30 1.29 1.50 -0.97 1.78 2.26 2.09 1.71 -10.33 1.18 1.44 1.42 1.62 -3.59
Nucleus 2.46 2.58 2.60 1.69 3.33 0.93 2.49 1.57 0.99 -5.11 2.54 2.81 2.74 1.90 -3.46
Peroxisomes -2.07 7.84 2.50 4.40 -4.58 4.91 20.19 10.99 6.56 -11.06 4.04 20.93 10.78 7.43 -11.59
Vacuole 1.10 -5.56 -3.62 -5.62 1.88 0.59 -6.71 -4.72 -7.03 2.48 1.16 -8.09 -5.27 -7.82 3.86
Vacuole Periphery -0.59 -5.93 -1.84 -1.00 -0.60 1.37 1.76 0.39 -2.52 -3.65 0.53 -0.79 -0.95 -1.51 -0.53
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
Localization
Cell Percentages ER (93%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sec16

Sec16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available