Standard name | |
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Description | COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 189 | 111 | 285 | 199 | 210 | 261 | 173 | 147 | 123 | 100 | 56 | 97 | 99 | 126 | 117 | 54 | 43 | 53 | 171 | 85 | 270 | 73 | 129 | 100 |
Bud | 11 | 10 | 63 | 47 | 66 | 53 | 101 | 71 | 48 | 54 | 56 | 60 | 9 | 11 | 22 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 13 | 40 | 44 | 42 | 49 | 31 | 21 | 21 | 36 | 22 | 37 | 16 | 9 | 17 | 11 | 10 | 14 | 2 | 2 | 13 | 2 | 5 | 7 |
Cytoplasm | 9 | 37 | 31 | 24 | 23 | 35 | 48 | 29 | 27 | 34 | 19 | 21 | 18 | 23 | 17 | 12 | 15 | 20 | 2 | 6 | 2 | 3 | 0 | 0 |
Endoplasmic Reticulum | 149 | 210 | 195 | 109 | 130 | 236 | 139 | 153 | 136 | 110 | 98 | 111 | 124 | 132 | 117 | 11 | 7 | 4 | 104 | 112 | 97 | 39 | 44 | 52 |
Endosome | 9 | 3 | 15 | 0 | 16 | 5 | 23 | 21 | 2 | 2 | 0 | 2 | 1 | 1 | 3 | 3 | 1 | 0 | 6 | 6 | 8 | 1 | 1 | 1 |
Golgi | 207 | 18 | 41 | 9 | 25 | 5 | 6 | 1 | 3 | 2 | 3 | 3 | 49 | 78 | 43 | 88 | 71 | 79 | 71 | 51 | 97 | 15 | 22 | 23 |
Mitochondria | 21 | 19 | 126 | 227 | 183 | 358 | 496 | 576 | 502 | 546 | 415 | 611 | 5 | 28 | 88 | 6 | 9 | 10 | 4 | 12 | 28 | 6 | 4 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 2 | 3 | 2 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 26 | 4 | 52 | 16 | 40 | 41 | 12 | 38 | 18 | 24 | 3 | 5 | 5 | 1 | 0 | 1 | 3 | 1 | 17 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 4 | 0 | 4 | 3 | 1 | 6 | 7 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 1 | 2 | 0 | 2 | 1 | 3 | 18 | 0 | 2 | 1 | 2 | 0 | 1 | 1 | 1 | 3 | 2 | 0 | 5 | 2 | 2 | 0 | 0 |
Unique Cell Count | 333 | 281 | 476 | 367 | 433 | 603 | 691 | 762 | 607 | 656 | 490 | 702 | 194 | 254 | 262 | 119 | 106 | 118 | 369 | 293 | 546 | 149 | 214 | 193 |
Labelled Cell Count | 614 | 423 | 824 | 663 | 753 | 1024 | 1065 | 1085 | 880 | 941 | 701 | 975 | 328 | 415 | 434 | 188 | 160 | 185 | 369 | 293 | 546 | 149 | 214 | 193 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 10.3 | 7.6 | 6.8 | 7.2 | 6.3 | 6.0 | 5.9 | 5.3 | 5.2 | 4.9 | 4.8 | 10.6 | 10.4 | 9.3 | 12.1 | 14.0 | 13.0 | 10.9 | 12.1 | 12.4 |
Std Deviation (1e-4) | 1.9 | 1.7 | 1.0 | 1.1 | 1.0 | 0.9 | 0.9 | 1.0 | 1.0 | 1.0 | 0.9 | 0.9 | 1.7 | 1.9 | 1.6 | 3.0 | 2.5 | 2.6 | 2.2 | 2.0 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.07 | -0.26 | -0.33 | -0.35 | -0.52 | -0.54 | -0.62 | -0.65 | 0.48 | 0.46 | 0.31 | 0.68 | 0.89 | 0.79 | 0.52 | 0.68 | 0.71 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.6 | -3.4 | -5.4 | -12.0 | -14.6 | -13.4 | -15.6 | -15.8 | 0 | -2.1 | -2.7 | -4.0 | -2.9 | -3.6 | -2.9 |
Bud | -0.2 | 0.9 | -2.3 | 0.7 | -2.2 | -2.9 | -2.7 | -0.9 | 0 | -3.3 | -3.8 | -2.0 | -4.2 | -3.7 | -3.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.7 | 0.7 | -0.2 | -2.8 | -4.5 | -3.5 | -1.9 | -2.5 | 0 | -0.1 | -2.5 | -0.9 | 0.3 | 0.3 | 1.2 |
Cytoplasm | 0 | -0.8 | -0.5 | 0.3 | -2.2 | -1.5 | -0.9 | -1.8 | -2.9 | 1.2 | 1.3 | 0 | 1.3 | 2.6 | 3.6 |
Endoplasmic Reticulum | -3.4 | -3.4 | -0.6 | -7.7 | -8.0 | -6.6 | -9.1 | -7.1 | 0 | 5.4 | 2.8 | 1.0 | -6.5 | -6.7 | -7.7 |
Endosome | -3.4 | 0.5 | -2.8 | 0.2 | -0.4 | -3.7 | -3.9 | -4.0 | 0 | 0 | -2.4 | -1.7 | 0 | 0 | 0 |
Golgi | -3.8 | -1.6 | -6.3 | -6.6 | -8.0 | -6.7 | -7.2 | -6.0 | 0 | 5.7 | 7.7 | 3.2 | 15.5 | 13.8 | 14.1 |
Mitochondria | 10.3 | 5.0 | 10.8 | 15.2 | 17.0 | 18.6 | 19.2 | 18.2 | 0 | -7.1 | -4.9 | 2.0 | -5.0 | -4.0 | -4.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -3.4 | 3.5 | -2.4 | 0.2 | -0.1 | -3.1 | 0.2 | -1.3 | 0 | -2.3 | -2.2 | -2.3 | -2.2 | 0 | -2.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |