Standard name
Description COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.57 0.4 0.6 0.54 0.48 0.43 0.25 0.19 0.2 0.15 0.11 0.14 0.51 0.5 0.45 0.45 0.41 0.45 0.46 0.29 0.49 0.5 0.6 0.52
Bud 0 0 0.13 0.13 0.15 0.09 0.15 0.09 0.08 0.08 0.11 0.09 0.05 0 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.08 0.12 0.1 0.08 0 0 0 0.05 0 0.05 0.08 0 0.06 0.09 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0 0.13 0.07 0.07 0.05 0.06 0.07 0 0 0.05 0 0 0.09 0.09 0.06 0.1 0.14 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.45 0.75 0.41 0.3 0.3 0.39 0.2 0.2 0.22 0.17 0.2 0.16 0.64 0.52 0.45 0.09 0.07 0 0.28 0.38 0.18 0.26 0.21 0.27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.62 0.06 0.09 0 0.06 0 0 0 0 0 0 0 0.25 0.31 0.16 0.74 0.67 0.67 0.19 0.18 0.18 0.1 0.11 0.12
Mitochondria 0.06 0.07 0.26 0.62 0.42 0.59 0.72 0.76 0.83 0.83 0.85 0.87 0 0.11 0.34 0.05 0.08 0.08 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.12 0 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 189 111 285 199 210 261 173 147 123 100 56 97 99 126 117 54 43 53 171 85 270 73 129 100
Bud 11 10 63 47 66 53 101 71 48 54 56 60 9 11 22 0 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 1 1 7 1 1 0 0 0 0 0 0
Cell Periphery 14 13 40 44 42 49 31 21 21 36 22 37 16 9 17 11 10 14 2 2 13 2 5 7
Cytoplasm 9 37 31 24 23 35 48 29 27 34 19 21 18 23 17 12 15 20 2 6 2 3 0 0
Endoplasmic Reticulum 149 210 195 109 130 236 139 153 136 110 98 111 124 132 117 11 7 4 104 112 97 39 44 52
Endosome 9 3 15 0 16 5 23 21 2 2 0 2 1 1 3 3 1 0 6 6 8 1 1 1
Golgi 207 18 41 9 25 5 6 1 3 2 3 3 49 78 43 88 71 79 71 51 97 15 22 23
Mitochondria 21 19 126 227 183 358 496 576 502 546 415 611 5 28 88 6 9 10 4 12 28 6 4 2
Nucleus 0 0 0 0 0 1 0 0 3 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 3 0 2 1 2 3 2 1 0 4 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 26 4 52 16 40 41 12 38 18 24 3 5 5 1 0 1 3 1 17 0 1 0
SpindlePole 0 0 0 0 4 0 4 3 1 6 7 2 0 1 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 4 1 2 0 2 1 3 18 0 2 1 2 0 1 1 1 3 2 0 5 2 2 0 0
Unique Cell Count 333 281 476 367 433 603 691 762 607 656 490 702 194 254 262 119 106 118 369 293 546 149 214 193
Labelled Cell Count 614 423 824 663 753 1024 1065 1085 880 941 701 975 328 415 434 188 160 185 369 293 546 149 214 193


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 10.3 7.6 6.8 7.2 6.3 6.0 5.9 5.3 5.2 4.9 4.8 10.6 10.4 9.3 12.1 14.0 13.0 10.9 12.1 12.4
Std Deviation (1e-4) 1.9 1.7 1.0 1.1 1.0 0.9 0.9 1.0 1.0 1.0 0.9 0.9 1.7 1.9 1.6 3.0 2.5 2.6 2.2 2.0 2.1
Intensity Change (Log2) -0.15 -0.07 -0.26 -0.33 -0.35 -0.52 -0.54 -0.62 -0.65 0.48 0.46 0.31 0.68 0.89 0.79 0.52 0.68 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -3.4 -5.4 -12.0 -14.6 -13.4 -15.6 -15.8 0 -2.1 -2.7 -4.0 -2.9 -3.6 -2.9
Bud -0.2 0.9 -2.3 0.7 -2.2 -2.9 -2.7 -0.9 0 -3.3 -3.8 -2.0 -4.2 -3.7 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 0.7 -0.2 -2.8 -4.5 -3.5 -1.9 -2.5 0 -0.1 -2.5 -0.9 0.3 0.3 1.2
Cytoplasm 0 -0.8 -0.5 0.3 -2.2 -1.5 -0.9 -1.8 -2.9 1.2 1.3 0 1.3 2.6 3.6
Endoplasmic Reticulum -3.4 -3.4 -0.6 -7.7 -8.0 -6.6 -9.1 -7.1 0 5.4 2.8 1.0 -6.5 -6.7 -7.7
Endosome -3.4 0.5 -2.8 0.2 -0.4 -3.7 -3.9 -4.0 0 0 -2.4 -1.7 0 0 0
Golgi -3.8 -1.6 -6.3 -6.6 -8.0 -6.7 -7.2 -6.0 0 5.7 7.7 3.2 15.5 13.8 14.1
Mitochondria 10.3 5.0 10.8 15.2 17.0 18.6 19.2 18.2 0 -7.1 -4.9 2.0 -5.0 -4.0 -4.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.4 3.5 -2.4 0.2 -0.1 -3.1 0.2 -1.3 0 -2.3 -2.2 -2.3 -2.2 0 -2.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0