ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.5 0.57 0.4 0.58 0.43 – 0.24 0.26 0.18 0.2 0.2 0.77 0.85 0.9 0.54 0.49 0.43 0.6 0.46 0.49 0.59 0.56 0.55
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.38 0.6 0.13 0.52 – 0.61 0.7 0.71 0.74 0.75 0 0 0 0 0 0.07 0 0 0.07 0 0 0
Nucleus 0 0 0.05 0 0.1 0.14 – 0.28 0.26 0.28 0.29 0.27 0 0 0 0.1 0.08 0.11 0.15 0.26 0.15 0.16 0.13 0.14
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.46 0.17 0.19 0.27 0.19 – 0.2 0.13 0.1 0.1 0.1 0.33 0.2 0.11 0.38 0.47 0.45 0.13 0.21 0.2 0.16 0.18 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 1 0 2 0 0 2 – 3 1 5 3 3 0 0 0 0 0 0 8 1 1 1 6 9
Bud Neck 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 3 0 2 0 3 7
Bud Site 0 0 0 0 0 0 – 4 0 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 – 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0
Cytoplasm 258 12 46 17 67 98 – 71 43 37 51 47 153 311 370 70 24 43 274 35 66 115 131 137
Endoplasmic Reticulum 3 1 0 1 0 0 – 0 0 0 1 0 0 3 3 7 3 3 1 0 0 0 0 0
Endosome 9 0 0 0 6 0 – 1 0 1 0 0 0 7 2 7 1 6 5 0 1 0 3 1
Golgi 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 2 0 1 2 0 0 1 1 2
Mitochondria 31 1 31 26 15 119 – 178 117 147 186 177 1 7 3 3 1 7 5 0 9 4 3 2
Nucleus 8 1 4 0 12 31 – 83 44 58 72 64 8 5 0 13 4 11 69 20 19 31 31 35
Nuclear Periphery 0 0 0 1 0 0 – 3 0 0 1 3 0 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 1 1 0 1 0 1 – 0 0 3 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 12 1 1 3 6 4
Vac/Vac Membrane 140 11 14 8 31 43 – 58 22 21 24 24 65 73 47 50 23 45 61 16 27 30 41 41
Unique Cell Count 386 24 81 43 115 228 294 168 207 252 236 199 368 409 130 49 100 460 78 136 195 237 248
Labelled Cell Count 453 28 99 54 131 294 401 227 274 339 320 227 407 428 153 56 119 460 78 136 195 237 248


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.8 3.6 4.2 4.7 3.7 – 3.9 3.5 3.9 3.6 3.3 4.8 5.1 4.8 6.8 6.8 6.7 4.7 5.3 5.4
Std Deviation (1e-4) 0.6 0.6 0.6 2.0 1.5 1.5 – 1.4 1.3 3.2 1.2 0.7 0.7 1.0 0.9 1.5 1.9 1.9 0.8 1.5 1.4
Intensity Change (Log2) – – – 0.23 0.39 0.06 – 0.14 -0.03 0.13 0.03 -0.08 0.44 0.52 0.45 0.94 0.95 0.91 0.39 0.58 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.3 3.6 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6677 -0.3774 -0.3955 -0.5624 -0.4102 -0.3803 3.7872 1.8376 2.7542 -0.9114 -0.9023 3.1179 0.9023 0.8451 0.633 0.625 0.7408 0.8609
Actin 0.0326 0.0001 0.0022 0.0131 0.0116 0.0004 0.0271 0.0004 0.0033 0.0076 0.0008 0.0009 0.0318 0.0001 0.0117 0.0004 0.0553 0.0137
Bud 0.0003 0.0001 0.001 0.0001 0.0003 0.0001 0.0029 0.0098 0.001 0.001 0.0011 0.0005 0.0006 0.0002 0 0.0001 0.0008 0.0004
Bud Neck 0.0013 0.0018 0.0026 0.0002 0.0001 0.0026 0.0078 0.0002 0.0003 0.0004 0.0005 0.002 0.0008 0.0001 0.0001 0.0002 0.0003 0.0018
Bud Periphery 0.0004 0.0002 0.0013 0.0002 0.0001 0.0001 0.0032 0.0027 0.0003 0.0007 0.0012 0.0005 0.0013 0.0003 0 0.0001 0.0017 0.0006
Bud Site 0.0028 0.0008 0.0009 0.0003 0.0002 0.0002 0.0117 0.0073 0.0009 0.002 0.0015 0.0019 0.0019 0.0005 0.0001 0 0.0024 0.0005
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.001 0.0005 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0 0 0.0001 0.0001
Cytoplasm 0.1317 0.2204 0.2469 0.2111 0.1553 0.3566 0.1968 0.3297 0.2863 0.1943 0.2432 0.3716 0.1198 0.2189 0.158 0.223 0.1531 0.2514
Cytoplasmic Foci 0.0341 0.0048 0.016 0.0041 0.0294 0.0178 0.0336 0.0071 0.0165 0.0288 0.0183 0.0242 0.0498 0.0044 0.0056 0.0032 0.0038 0.0074
Eisosomes 0.0007 0.0001 0.0001 0.0006 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0007 0.0001 0.0005 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0037 0.0023 0.0036 0.0023 0.0013 0.0019 0.0069 0.0032 0.003 0.0048 0.0043 0.0046 0.0084 0.002 0.001 0.007 0.0024 0.0052
Endosome 0.0202 0.0071 0.0076 0.009 0.0077 0.0053 0.0318 0.0028 0.0049 0.0104 0.0812 0.0102 0.0158 0.0012 0.0009 0.0029 0.0062 0.0071
Golgi 0.0071 0.0009 0.0029 0.0031 0.0137 0.0016 0.0097 0.0002 0.0007 0.0104 0.0316 0.0013 0.0047 0.0001 0.0006 0.0001 0.0068 0.0028
Lipid Particles 0.0159 0.0004 0.0018 0.0247 0.0146 0.0006 0.0125 0.0007 0.0019 0.0197 0.0246 0.001 0.0202 0.0002 0.0005 0.0003 0.0073 0.0023
Mitochondria 0.0037 0.0003 0.0101 0.0029 0.004 0.001 0.0066 0.0004 0.0012 0.0099 0.0108 0.0013 0.0042 0.0004 0.0005 0.001 0.017 0.0016
None 0.6867 0.7148 0.6334 0.6795 0.7344 0.5306 0.5628 0.5861 0.6077 0.6372 0.4715 0.4626 0.6107 0.6904 0.7942 0.7216 0.7275 0.5955
Nuclear Periphery 0.0135 0.0025 0.0053 0.0162 0.0013 0.0036 0.015 0.0045 0.0048 0.0132 0.0095 0.0093 0.0133 0.0036 0.0016 0.0067 0.0052 0.0089
Nucleolus 0.0013 0.0005 0.0005 0.0016 0.0002 0.0008 0.0018 0.0007 0.0012 0.001 0.0005 0.0013 0.0012 0.0008 0.0004 0.0009 0.0003 0.0008
Nucleus 0.0073 0.0336 0.0469 0.0132 0.0048 0.065 0.0367 0.0334 0.045 0.0264 0.0661 0.0876 0.0397 0.0671 0.0208 0.0202 0.0027 0.0855
Peroxisomes 0.0149 0.0001 0.0053 0.0051 0.0106 0.0018 0.0098 0.0003 0.0067 0.0119 0.009 0.0003 0.0434 0.0002 0.0003 0.0002 0.0031 0.0016
Punctate Nuclear 0.0178 0.0034 0.0084 0.0104 0.0098 0.009 0.0051 0.006 0.0105 0.0168 0.008 0.0147 0.0281 0.0079 0.0027 0.0107 0.0019 0.0108
Vacuole 0.003 0.0053 0.0022 0.0007 0.0004 0.0008 0.0134 0.0034 0.0026 0.0023 0.011 0.0031 0.0023 0.0013 0.0004 0.001 0.0006 0.0015
Vacuole Periphery 0.001 0.0003 0.0011 0.0013 0.0002 0.0002 0.0035 0.0004 0.0006 0.0011 0.0051 0.001 0.0012 0.0002 0.0001 0.0005 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1994 10.4711 13.986 10.9444 13.6683 9.3656 10.5873 16.4351 17.158 14.1986
Translational Efficiency 0.901 0.6715 0.5973 0.7044 0.3555 1.1842 0.8369 0.4768 0.3235 0.3859

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘