ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.5 0.57 0.4 0.58 0.43 0.24 0.26 0.18 0.2 0.2 0.77 0.85 0.9 0.54 0.49 0.43 0.6 0.46 0.49 0.59 0.56 0.55
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.38 0.6 0.13 0.52 0.61 0.7 0.71 0.74 0.75 0 0 0 0 0 0.07 0 0 0.07 0 0 0
Nucleus 0 0 0.05 0 0.1 0.14 0.28 0.26 0.28 0.29 0.27 0 0 0 0.1 0.08 0.11 0.15 0.26 0.15 0.16 0.13 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.46 0.17 0.19 0.27 0.19 0.2 0.13 0.1 0.1 0.1 0.33 0.2 0.11 0.38 0.47 0.45 0.13 0.21 0.2 0.16 0.18 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 1 0 2 0 0 2 3 1 5 3 3 0 0 0 0 0 0 8 1 1 1 6 9
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 2 0 3 7
Bud Site 0 0 0 0 0 0 4 0 1 1 2 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0
Cytoplasm 258 12 46 17 67 98 71 43 37 51 47 153 311 370 70 24 43 274 35 66 115 131 137
Endoplasmic Reticulum 3 1 0 1 0 0 0 0 0 1 0 0 3 3 7 3 3 1 0 0 0 0 0
Endosome 9 0 0 0 6 0 1 0 1 0 0 0 7 2 7 1 6 5 0 1 0 3 1
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 2 0 0 1 1 2
Mitochondria 31 1 31 26 15 119 178 117 147 186 177 1 7 3 3 1 7 5 0 9 4 3 2
Nucleus 8 1 4 0 12 31 83 44 58 72 64 8 5 0 13 4 11 69 20 19 31 31 35
Nuclear Periphery 0 0 0 1 0 0 3 0 0 1 3 0 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 1 1 0 1 0 1 0 0 3 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 1 1 3 6 4
Vac/Vac Membrane 140 11 14 8 31 43 58 22 21 24 24 65 73 47 50 23 45 61 16 27 30 41 41
Unique Cell Count 386 24 81 43 115 228 294 168 207 252 236 199 368 409 130 49 100 460 78 136 195 237 248
Labelled Cell Count 453 28 99 54 131 294 401 227 274 339 320 227 407 428 153 56 119 460 78 136 195 237 248


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.8 3.6 4.2 4.7 3.7 3.9 3.5 3.9 3.6 3.3 4.8 5.1 4.8 6.8 6.8 6.7 4.7 5.3 5.4
Std Deviation (1e-4) 0.6 0.6 0.6 2.0 1.5 1.5 1.4 1.3 3.2 1.2 0.7 0.7 1.0 0.9 1.5 1.9 1.9 0.8 1.5 1.4
Intensity Change (Log2) 0.23 0.39 0.06 0.14 -0.03 0.13 0.03 -0.08 0.44 0.52 0.45 0.94 0.95 0.91 0.39 0.58 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 3.6 4.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl071c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available