Standard name
Human Ortholog
Description Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.05 0.09 0.13 0.1 0.09 0.1 0.06 0.06 0.07 0.15 0.14 0.11 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.5 0 0.05 0 0.28 0.51 0.14 0.69 0.45 0.42 0.55 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Nucleus 0.93 0.79 0.94 0.93 0.92 0.85 0.63 0.77 0.68 0.79 0.78 0.72 0.91 0.82 0.84 0.83 0.76 0.8 0.91 0.8 0.91 0.82 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0.09 0.11 0.07 0 0.11 0.08 0.1 0.12 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 5 0 4 5 3 4 0 0 0 1 0 0 2 1 0 1 0 2
Bud Neck 0 0 0 0 0 0 1 3 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 2 12 8 6 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 19 4 3 8 4 18 17 16 15 16 19 9 34 35 29 19 22 4 5 0 6 2 7
Endoplasmic Reticulum 0 2 0 0 0 0 0 2 0 0 0 0 0 1 2 9 10 5 0 0 0 0 0 0
Endosome 0 1 0 1 0 1 0 1 0 0 0 1 0 1 0 3 0 2 0 7 0 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0
Mitochondria 20 207 3 6 4 13 69 22 122 69 114 175 5 4 2 15 14 8 2 11 0 1 2 7
Nucleus 262 328 92 113 153 39 85 124 120 122 211 231 116 182 205 222 133 160 254 355 101 172 132 129
Nuclear Periphery 0 0 0 0 0 0 2 1 0 1 0 0 0 0 1 2 0 2 0 0 0 0 0 0
Nucleolus 0 0 1 1 1 0 1 0 1 2 3 4 0 0 1 1 0 1 0 0 0 0 4 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 1 1 2 0 2 0 3 1 7 0 2 3 0 1 1 0 7 0 2 3 3
Vac/Vac Membrane 10 7 1 2 3 0 1 11 4 1 5 9 1 9 3 23 20 15 12 49 9 21 20 44
Unique Cell Count 282 415 98 121 167 46 134 162 177 155 270 320 128 223 244 269 176 201 281 446 112 210 172 207
Labelled Cell Count 302 565 102 127 171 61 185 185 280 227 359 467 131 233 252 306 198 216 281 446 112 210 172 207


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.7 5.5 5.3 5.9 4.6 3.4 5.2 3.8 4.3 4.2 4.1 5.4 5.5 5.4 4.9 5.9 6.5 5.7 6.2 6.2
Std Deviation (1e-4) 0.9 0.7 1.0 1.3 1.3 1.2 1.1 1.7 1.1 1.2 1.3 0.8 0.9 1.3 1.1 1.2 1.3 1.7 1.0 1.4 1.1
Intensity Change (Log2) – – – -0.06 0.1 -0.26 -0.68 -0.07 -0.55 -0.35 -0.39 -0.43 -0.04 -0.0 -0.04 -0.16 0.09 0.24 0.04 0.17 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.1 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4222 4.4672 4.4912 4.1159 3.8727 4.3684 4.1423 4.665 3.9241 4.156 4.1738 4.0224 2.2997 2.0175 1.9613 2.181 1.6801 1.7932
Actin 0.0441 0.0001 0.0124 0.0014 0.0233 0.0114 0.02 0.0001 0.0017 0 0.0016 0.0001 0.0587 0.0001 0.0126 0.0398 0.0021 0.0043
Bud 0.0004 0 0.0002 0 0.0004 0.0003 0.0003 0 0.0003 0 0.0004 0.0001 0.001 0 0.0003 0.0006 0.0001 0.0002
Bud Neck 0.006 0.0001 0.0012 0.0002 0.001 0.0015 0.0024 0.0001 0.0046 0.0001 0.0003 0.0009 0.0192 0.0003 0.0006 0.0016 0.0007 0.0012
Bud Periphery 0.0006 0 0.0003 0.0001 0.0028 0.0013 0.0006 0 0.0007 0 0.0005 0.0001 0.0012 0 0.0004 0.0023 0.0001 0.0004
Bud Site 0.0118 0.0001 0.005 0.0001 0.0004 0.0014 0.0066 0 0.0057 0 0.0006 0.0001 0.0287 0.0002 0.0066 0.0049 0.0001 0.0005
Cell Periphery 0.0006 0 0.0008 0 0.0001 0.0001 0.0004 0 0.0004 0 0.0001 0 0.0004 0 0.0001 0.0029 0 0.0001
Cytoplasm 0.0043 0.0012 0.0069 0.0004 0.0002 0.0023 0.0092 0.0031 0.003 0.0001 0.0045 0.0006 0.0197 0.0112 0.003 0.0143 0.0033 0.0018
Cytoplasmic Foci 0.0149 0 0.003 0.0005 0.0014 0.0015 0.0193 0.0003 0.0023 0 0.0111 0.0007 0.026 0.0003 0.0118 0.0013 0.001 0.0027
Eisosomes 0.0008 0 0.0001 0 0.0002 0.0002 0.0003 0 0.0002 0 0 0 0.0004 0 0.0002 0.0004 0 0
Endoplasmic Reticulum 0.0022 0.0005 0.0017 0 0.0007 0.0041 0.0026 0.0001 0.0008 0 0.0044 0 0.0037 0.0008 0.0002 0.0093 0.002 0.0005
Endosome 0.0236 0.0001 0.006 0.0009 0.0039 0.0087 0.0093 0.0001 0.0015 0 0.0243 0.0003 0.0198 0.0009 0.0047 0.0021 0.017 0.0059
Golgi 0.0103 0 0.0053 0.0014 0.0064 0.0021 0.0031 0 0.0006 0 0.0032 0.0003 0.0123 0 0.0041 0.0057 0.0008 0.0025
Lipid Particles 0.0139 0 0.0029 0.0013 0.007 0.0013 0.0057 0.0001 0.0016 0 0.0088 0.0044 0.0184 0.0001 0.0093 0.002 0.0004 0.0052
Mitochondria 0.0033 0.0005 0.0028 0.0302 0.0083 0.003 0.0018 0.0002 0.003 0.0001 0.0114 0.0004 0.0086 0.0003 0.0211 0.0014 0.0014 0.0074
None 0.0376 0.0061 0.0138 0.0002 0.0003 0.0006 0.0289 0.0655 0.0075 0 0.0006 0.0052 0.0123 0.0169 0.0017 0.0039 0.0018 0.0002
Nuclear Periphery 0.0057 0.0116 0.0095 0.0014 0.0143 0.0072 0.0082 0.0086 0.0054 0.0003 0.0064 0.0005 0.0125 0.0083 0.0018 0.0033 0.0245 0.0019
Nucleolus 0.0055 0.0072 0.0049 0.0076 0.0197 0.0092 0.0078 0.0051 0.0044 0.0044 0.0038 0.0091 0.0056 0.0054 0.0041 0.0024 0.0091 0.0097
Nucleus 0.7932 0.9681 0.9155 0.9306 0.8887 0.9311 0.841 0.9087 0.9455 0.9946 0.8673 0.9677 0.6762 0.9505 0.9052 0.8947 0.9292 0.9497
Peroxisomes 0.0105 0 0.0042 0.0022 0.0037 0.0014 0.0081 0 0.0033 0 0.043 0.0052 0.0449 0 0.0095 0.0015 0.0002 0.0032
Punctate Nuclear 0.0086 0.0037 0.0022 0.0211 0.0113 0.0061 0.0208 0.0076 0.0057 0.0004 0.0029 0.0039 0.0277 0.0038 0.0014 0.0045 0.0037 0.0013
Vacuole 0.0012 0.0002 0.0011 0.0001 0.0017 0.0039 0.0029 0.0002 0.0015 0 0.0031 0.0002 0.002 0.0006 0.0011 0.001 0.0011 0.0009
Vacuole Periphery 0.0006 0.0002 0.0004 0.0001 0.0041 0.0014 0.0007 0.0001 0.0003 0 0.0019 0 0.0007 0.0002 0.0003 0.0002 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.0057 3.2661 12.926 18.7102 17.3695 12.1721 14.5856 21.9175 22.2998 17.6117
Translational Efficiency 0.5585 2.7396 0.7783 0.4797 0.499 0.7851 0.6704 0.5746 0.5389 0.619

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2527 1036 1410 1613 1610 1939 228 1716 4137 2975 1638 3329

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 771.63 793.71 852.15 892.08 659.82 852.61 954.41 926.45 728.12 832.10 866.38 909.80
Standard Deviation 90.14 111.23 110.31 133.55 91.79 103.62 109.73 125.03 105.89 109.97 115.77 130.37
Intensity Change Log 2 — 0.040703 0.143198 0.209264 — 0.369813 0.532537 0.489641 — 0.201768 0.335768 0.345295

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000035 0.000234 0.000328 0.001536 0.000102 0.000123 0.000460 0.001098 0.000061 0.000162 0.000346 0.001310
Bud Neck 0.002427 0.003466 0.006360 0.015867 0.001658 0.004729 0.004434 0.014938 0.002127 0.004289 0.006092 0.015388
Bud Site 0.000731 0.000945 0.001184 0.014629 0.000819 0.003268 0.000667 0.005870 0.000765 0.002459 0.001112 0.010114
Cell Periphery 0.000110 0.000108 0.000078 0.000176 0.000157 0.000191 0.000057 0.000131 0.000128 0.000162 0.000075 0.000153
Cytoplasm 0.035944 0.027846 0.005101 0.029310 0.047175 0.030546 0.002893 0.022775 0.040315 0.029606 0.004794 0.025941
Cytoplasmic Foci 0.002420 0.003654 0.000120 0.003892 0.004878 0.006318 0.000035 0.001326 0.003377 0.005390 0.000108 0.002569
Eisosomes 0.000010 0.000017 0.000017 0.000028 0.000017 0.000021 0.000023 0.000020 0.000013 0.000019 0.000018 0.000024
Endoplasmic Reticulum 0.001235 0.004852 0.004506 0.007894 0.001526 0.003059 0.005610 0.007341 0.001348 0.003683 0.004659 0.007609
Endosome 0.000293 0.001454 0.000251 0.004110 0.000763 0.002006 0.000182 0.001229 0.000475 0.001814 0.000242 0.002625
Golgi 0.000081 0.000299 0.000029 0.004783 0.000240 0.000555 0.000026 0.001019 0.000143 0.000466 0.000028 0.002843
Lipid Particles 0.000557 0.001450 0.000140 0.000524 0.001335 0.001546 0.000138 0.000185 0.000860 0.001513 0.000140 0.000349
Mitochondria 0.001858 0.002197 0.000678 0.006702 0.002188 0.004917 0.000597 0.003272 0.001987 0.003970 0.000666 0.004934
Mitotic Spindle 0.000810 0.001500 0.000603 0.029203 0.001357 0.002667 0.000070 0.008788 0.001023 0.002261 0.000529 0.018680
None 0.003099 0.008073 0.003079 0.004394 0.013467 0.007300 0.003097 0.005125 0.007134 0.007569 0.003082 0.004771
Nuclear Periphery 0.001504 0.000795 0.000540 0.003249 0.000589 0.001335 0.000445 0.001566 0.001148 0.001147 0.000527 0.002381
Nuclear Periphery Foci 0.000107 0.000325 0.000085 0.001669 0.000693 0.000209 0.000057 0.000236 0.000335 0.000249 0.000081 0.000930
Nucleolus 0.001731 0.003440 0.001983 0.001414 0.003871 0.003629 0.001886 0.001950 0.002564 0.003563 0.001969 0.001690
Nucleus 0.944999* 0.931816* 0.973025* 0.847477* 0.916765* 0.919381* 0.977126* 0.913869* 0.934011* 0.923711* 0.973596* 0.881700*
Peroxisomes 0.000368 0.001247 0.000067 0.000789 0.000886 0.001784 0.000072 0.000143 0.000570 0.001597 0.000068 0.000456
Vacuole 0.001519 0.005807 0.001713 0.016433 0.001258 0.005665 0.002071 0.007705 0.001417 0.005715 0.001763 0.011934
Vacuole Periphery 0.000163 0.000474 0.000115 0.005923 0.000257 0.000751 0.000055 0.001415 0.000200 0.000654 0.000106 0.003599

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.32 -17.55 -5.68 -4.69 -4.09 -0.84 -6.28 -2.26 -2.21 -0.91 -5.82 -16.24 -4.37 -3.97 -2.98
Bud Neck -1.30 -4.90 -13.66 -10.44 -6.68 -4.04 -5.60 -14.39 -9.85 -6.60 -3.81 -6.01 -19.69 -14.11 -8.38
Bud Site -0.60 -1.63 -7.21 -7.12 -6.81 -3.30 -1.52 -5.39 -1.73 -4.30 -3.35 -2.06 -8.94 -5.88 -8.14
Cell Periphery 0.06 2.10 -1.62 -1.52 -3.65 -0.97 3.00 1.41 3.29 -3.00 -1.81 3.39 0.12 2.21 -4.28
Cytoplasm 2.35 14.97 2.53 -0.28 -10.99 4.59 13.59 7.38 3.24 -8.99 4.55 19.96 7.11 2.04 -13.99
Cytoplasmic Foci -1.48 6.56 1.27 2.19 -3.44 -1.00 7.07 5.60 6.37 -2.01 -2.69 9.25 5.21 6.37 -3.57
Eisosomes -5.20 -8.20 -15.55 -8.36 -8.67 -1.11 -3.34 -3.89 -3.07 1.30 -4.40 -5.92 -13.67 -7.13 -7.15
Endoplasmic Reticulum -11.79 -13.10 -16.94 -8.10 -8.00 -3.72 -7.61 -15.10 -9.25 -0.97 -8.12 -14.86 -22.72 -12.34 -8.62
Endosome -2.73 0.27 -4.36 -1.30 -4.39 -2.39 2.02 -0.48 2.19 -3.21 -3.95 2.13 -4.27 0.53 -5.43
Golgi -2.34 1.61 -3.26 -2.87 -3.34 -1.38 2.64 -0.97 -0.00 -1.70 -2.29 3.17 -3.41 -2.34 -3.76
Lipid Particles -1.60 4.05 0.82 1.78 -4.71 -0.40 3.88 3.74 4.02 -1.04 -2.05 5.39 3.89 4.05 -4.64
Mitochondria -0.42 2.50 -3.71 -2.57 -6.33 -2.20 1.80 -0.63 1.87 -3.37 -2.51 3.06 -2.95 0.29 -6.92
Mitotic Spindle -0.70 -0.03 -8.10 -7.42 -8.10 -1.22 -0.44 -5.15 -4.15 -3.54 -1.69 0.01 -9.68 -8.34 -9.65
None -3.92 0.14 -2.56 2.81 -2.08 3.45 6.63 5.30 2.15 -3.03 -0.43 5.24 3.39 3.47 -3.04
Nuclear Periphery 3.71 4.59 -5.29 -7.88 -8.37 -5.88 -1.81 -15.33 -6.68 -10.57 -0.03 3.97 -8.86 -9.43 -12.76
Nuclear Periphery Foci -2.85 -0.30 -2.67 -2.02 -2.64 1.65 1.72 0.86 -2.61 -2.34 0.79 1.77 -1.80 -2.75 -3.49
Nucleolus -3.30 -0.89 0.41 3.67 1.42 0.57 3.81 3.33 2.86 -0.62 -2.22 1.91 2.69 4.48 0.70
Nucleus 2.30 -7.40 12.80 8.82 17.32 -0.53 -7.50 1.50 2.10 9.47 2.40 -11.53 11.22 7.83 20.86
Peroxisomes -2.54 3.27 -0.45 2.21 -2.51 -1.76 2.86 2.84 4.53 -0.22 -3.42 4.11 2.14 4.77 -2.72
Vacuole -2.77 -1.83 -12.30 -7.77 -11.94 -5.32 -3.79 -9.60 -5.25 -5.45 -5.60 -3.59 -15.46 -9.78 -14.24
Vacuole Periphery -2.52 0.81 -3.55 -3.09 -3.61 -2.54 1.57 -1.95 -0.16 -2.89 -3.79 1.40 -4.06 -2.81 -4.30
Standard name
Human Ortholog
Description Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cwc27

Cwc27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwc27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available