Standard name | |
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Description | Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 4 | 5 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 12 | 8 | 6 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 19 | 4 | 3 | 8 | 4 | 18 | 17 | 16 | 15 | 16 | 19 | 9 | 34 | 35 | 29 | 19 | 22 | 4 | 5 | 0 | 6 | 2 | 7 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 9 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 7 | 0 | 0 | 1 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 20 | 207 | 3 | 6 | 4 | 13 | 69 | 22 | 122 | 69 | 114 | 175 | 5 | 4 | 2 | 15 | 14 | 8 | 2 | 11 | 0 | 1 | 2 | 7 |
Nucleus | 262 | 328 | 92 | 113 | 153 | 39 | 85 | 124 | 120 | 122 | 211 | 231 | 116 | 182 | 205 | 222 | 133 | 160 | 254 | 355 | 101 | 172 | 132 | 129 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 3 | 4 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 3 | 1 | 7 | 0 | 2 | 3 | 0 | 1 | 1 | 0 | 7 | 0 | 2 | 3 | 3 |
Vac/Vac Membrane | 10 | 7 | 1 | 2 | 3 | 0 | 1 | 11 | 4 | 1 | 5 | 9 | 1 | 9 | 3 | 23 | 20 | 15 | 12 | 49 | 9 | 21 | 20 | 44 |
Unique Cell Count | 282 | 415 | 98 | 121 | 167 | 46 | 134 | 162 | 177 | 155 | 270 | 320 | 128 | 223 | 244 | 269 | 176 | 201 | 281 | 446 | 112 | 210 | 172 | 207 |
Labelled Cell Count | 302 | 565 | 102 | 127 | 171 | 61 | 185 | 185 | 280 | 227 | 359 | 467 | 131 | 233 | 252 | 306 | 198 | 216 | 281 | 446 | 112 | 210 | 172 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 3.7 | 5.5 | 5.3 | 5.9 | 4.6 | 3.4 | 5.2 | 3.8 | 4.3 | 4.2 | 4.1 | 5.4 | 5.5 | 5.4 | 4.9 | 5.9 | 6.5 | 5.7 | 6.2 | 6.2 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.0 | 1.3 | 1.3 | 1.2 | 1.1 | 1.7 | 1.1 | 1.2 | 1.3 | 0.8 | 0.9 | 1.3 | 1.1 | 1.2 | 1.3 | 1.7 | 1.0 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.1 | -0.26 | -0.68 | -0.07 | -0.55 | -0.35 | -0.39 | -0.43 | -0.04 | -0.0 | -0.04 | -0.16 | 0.09 | 0.24 | 0.04 | 0.17 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.6 | 3.1 | 2.3 |