Standard name
Description Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.05 0.09 0.13 0.1 0.09 0.1 0.06 0.06 0.07 0.15 0.14 0.11 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.5 0 0.05 0 0.28 0.51 0.14 0.69 0.45 0.42 0.55 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Nucleus 0.93 0.79 0.94 0.93 0.92 0.85 0.63 0.77 0.68 0.79 0.78 0.72 0.91 0.82 0.84 0.83 0.76 0.8 0.91 0.8 0.91 0.82 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0.09 0.11 0.07 0 0.11 0.08 0.1 0.12 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 5 0 4 5 3 4 0 0 0 1 0 0 2 1 0 1 0 2
Bud Neck 0 0 0 0 0 0 1 3 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 2 12 8 6 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 19 4 3 8 4 18 17 16 15 16 19 9 34 35 29 19 22 4 5 0 6 2 7
Endoplasmic Reticulum 0 2 0 0 0 0 0 2 0 0 0 0 0 1 2 9 10 5 0 0 0 0 0 0
Endosome 0 1 0 1 0 1 0 1 0 0 0 1 0 1 0 3 0 2 0 7 0 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0
Mitochondria 20 207 3 6 4 13 69 22 122 69 114 175 5 4 2 15 14 8 2 11 0 1 2 7
Nucleus 262 328 92 113 153 39 85 124 120 122 211 231 116 182 205 222 133 160 254 355 101 172 132 129
Nuclear Periphery 0 0 0 0 0 0 2 1 0 1 0 0 0 0 1 2 0 2 0 0 0 0 0 0
Nucleolus 0 0 1 1 1 0 1 0 1 2 3 4 0 0 1 1 0 1 0 0 0 0 4 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 1 1 2 0 2 0 3 1 7 0 2 3 0 1 1 0 7 0 2 3 3
Vac/Vac Membrane 10 7 1 2 3 0 1 11 4 1 5 9 1 9 3 23 20 15 12 49 9 21 20 44
Unique Cell Count 282 415 98 121 167 46 134 162 177 155 270 320 128 223 244 269 176 201 281 446 112 210 172 207
Labelled Cell Count 302 565 102 127 171 61 185 185 280 227 359 467 131 233 252 306 198 216 281 446 112 210 172 207


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.7 5.5 5.3 5.9 4.6 3.4 5.2 3.8 4.3 4.2 4.1 5.4 5.5 5.4 4.9 5.9 6.5 5.7 6.2 6.2
Std Deviation (1e-4) 0.9 0.7 1.0 1.3 1.3 1.2 1.1 1.7 1.1 1.2 1.3 0.8 0.9 1.3 1.1 1.2 1.3 1.7 1.0 1.4 1.1
Intensity Change (Log2) – – – -0.06 0.1 -0.26 -0.68 -0.07 -0.55 -0.35 -0.39 -0.43 -0.04 -0.0 -0.04 -0.16 0.09 0.24 0.04 0.17 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.1 2.3