Standard name
Human Ortholog
Description Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0.11 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.2 – – – 0 0.06 0.06 0.08 0.1 0.07
Mitochondria 0.91 0.91 0.87 0.92 0.91 0.86 0.89 0.87 0.92 0.93 0.95 0.94 0.89 0.9 0.87 – – – 0.82 0.8 0.75 0.73 0.71 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 0.08 0.07 0.1 0.07 0.09 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.1 0 0.09 0 0.11 0.14 0.12 0.17 0.18 0.11 0.07 0.13 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.07 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.16 0 0 0.06 0 0 0 0 0 0 0 0.11 0.09 0.11 – – – 0 0.08 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 1 1 0 1 1 2 2 3 – – – 3 3 4 5 5 11
Bud 0 0 4 2 1 1 3 8 4 8 9 0 2 1 0 – – – 1 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0
Bud Site 1 0 0 0 0 0 1 2 1 1 0 1 0 0 0 – – – – – – – – –
Cell Periphery 1 6 2 3 0 0 1 0 1 1 0 1 3 5 5 – – – 0 0 0 0 0 0
Cytoplasm 5 0 3 5 2 5 3 9 6 6 5 2 9 6 3 – – – 1 0 0 0 0 0
Endoplasmic Reticulum 0 2 0 0 1 1 0 1 2 2 0 0 5 5 1 – – – 0 0 0 0 1 1
Endosome 0 0 0 0 0 0 2 0 0 1 0 0 0 1 1 – – – 3 0 1 1 7 5
Golgi 2 21 1 3 4 1 0 0 0 0 0 0 39 34 67 – – – 8 13 6 11 26 22
Mitochondria 188 168 80 101 106 144 200 209 132 172 140 137 254 316 289 – – – 170 170 76 101 182 219
Nucleus 1 1 1 0 1 1 1 1 0 0 0 1 1 0 0 – – – 2 0 0 0 0 1
Nuclear Periphery 4 3 3 0 5 7 15 18 10 19 10 13 2 5 7 – – – 0 1 0 1 2 3
Nucleolus 21 6 8 4 13 24 28 40 26 20 10 19 7 10 3 – – – 3 1 2 1 4 2
Peroxisomes 2 0 4 0 1 1 2 3 0 0 1 0 1 7 5 – – – 1 2 7 6 10 12
SpindlePole 1 0 0 0 0 1 1 2 0 1 0 0 0 0 0 – – – 1 0 0 1 0 0
Vac/Vac Membrane 26 29 3 2 7 6 10 5 0 6 1 4 31 30 38 – – – 6 17 0 2 8 22
Unique Cell Count 207 185 92 110 117 167 224 239 143 184 147 145 285 352 334 207 213 101 140 257 312
Labelled Cell Count 252 237 109 120 141 192 267 299 183 237 177 179 356 422 422 207 213 101 140 257 312


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 17.6 15.0 16.1 14.9 13.9 13.1 11.5 12.0 11.1 10.4 11.6 17.8 17.9 17.4 – – – 14.5 15.4 16.1
Std Deviation (1e-4) 3.0 3.3 3.5 3.9 4.0 3.9 3.5 3.0 3.6 2.9 2.8 3.3 3.5 4.1 3.5 – – – 3.4 3.3 3.3
Intensity Change (Log2) – – – 0.11 -0.01 -0.1 -0.19 -0.38 -0.31 -0.43 -0.53 -0.37 0.25 0.26 0.22 – – – -0.05 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0.6 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 3.4 2.7 4.4 – – –
Mitochondria 1.1 0.8 -0.2 0.6 0.1 1.3 1.8 2.3 2.0 0.6 0.8 -0.1 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 1.2 1.4 1.2 2.0 1.2 1.7 0 0 0 – – –
Nucleolus -1.5 0.6 1.3 1.0 1.9 2.0 0.6 -0.5 1.0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 2.2 1.7 2.3 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.8962 16.417 16.4297 15.2402 14.6916 16.9901 19.3456 18.7628 18.3358 19.0969 17.3087 18.7716 22.3302 21.5178 20.9603 19.1646 21.0447 21.1898
Actin 0.0075 0.0036 0.0075 0.0065 0.0004 0.0002 0.0143 0.0048 0.0162 0.0046 0.0007 0.0067 0.0039 0.0016 0.0024 0.0208 0.0002 0.0004
Bud 0.0004 0.0002 0.0004 0.0131 0.0001 0 0.0003 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0017 0.0006 0 0
Bud Neck 0.0017 0.0001 0.0005 0 0 0 0.0002 0.0001 0.0002 0.0001 0 0 0.0002 0.0001 0 0.0008 0 0
Bud Periphery 0.0006 0.0004 0.0008 0.0178 0.0005 0 0.0005 0.0005 0.001 0.0004 0.0002 0.0001 0.0001 0.0002 0.0028 0.0009 0 0
Bud Site 0.0121 0.0004 0.001 0.0032 0.0001 0 0.0012 0.0038 0.0026 0.0001 0.0001 0 0.0005 0.0003 0.0009 0.0101 0 0
Cell Periphery 0.0011 0.0005 0.0007 0.0002 0.0004 0.0001 0.0003 0.0003 0.0009 0.0001 0.0001 0.0001 0.0003 0.0005 0.0002 0.0008 0 0.0001
Cytoplasm 0.0013 0 0.0001 0 0.0001 0 0.0008 0 0.0007 0.0002 0.0074 0.0001 0.0014 0 0.0001 0.0021 0 0.0002
Cytoplasmic Foci 0.0136 0.0008 0.0022 0.0002 0.0003 0 0.0139 0.0002 0.0029 0.0044 0.0019 0.0024 0.0035 0.0009 0.0054 0.0293 0.0076 0.0005
Eisosomes 0.0006 0.0005 0.0007 0.0002 0.0001 0.0001 0.0005 0.001 0.0003 0.0004 0.0001 0.0001 0.0003 0.0001 0.0002 0.002 0 0.0001
Endoplasmic Reticulum 0.0013 0 0.0004 0.0001 0.0001 0 0.0008 0.0002 0.0004 0.0006 0.0003 0.0002 0.0028 0 0.0001 0.0006 0 0.0001
Endosome 0.012 0.0031 0.004 0.0046 0.0024 0.0003 0.0073 0.0014 0.003 0.0049 0.0062 0.0028 0.0079 0.0035 0.0019 0.0018 0.0002 0.0013
Golgi 0.0132 0.0138 0.01 0.0017 0.0009 0.001 0.0058 0.0033 0.0058 0.0041 0.0006 0.0083 0.0017 0.0131 0.0014 0.0014 0.0003 0.0065
Lipid Particles 0.0155 0.0046 0.0039 0.0021 0.0025 0.0018 0.017 0.0009 0.0074 0.0136 0.0003 0.0082 0.0043 0.0102 0.0092 0.018 0.0195 0.0013
Mitochondria 0.8934 0.9513 0.9445 0.9358 0.9449 0.9908 0.9074 0.9702 0.9431 0.9463 0.9601 0.9624 0.9389 0.9482 0.9515 0.8727 0.9653 0.9789
None 0.0013 0 0.0001 0.0001 0.0001 0 0.0049 0 0.0008 0.0003 0.0003 0.0001 0.0042 0 0.0004 0.0082 0 0.0001
Nuclear Periphery 0.0041 0.0001 0.0045 0.0002 0.0002 0 0.0017 0.0013 0.0003 0.005 0.0034 0.0004 0.0077 0 0.0007 0.0013 0 0.0016
Nucleolus 0.0025 0.0001 0.0002 0.0001 0.0021 0 0.0012 0.0001 0.0018 0.0001 0.0008 0.0001 0.002 0.0028 0.0003 0.0114 0.0044 0.0014
Nucleus 0.001 0.0001 0.0003 0.0001 0.0016 0 0.0006 0.0001 0.0003 0.0002 0.0055 0 0.0017 0.0005 0.0002 0.003 0.0002 0.0022
Peroxisomes 0.0066 0.009 0.0025 0.0014 0.0005 0.0002 0.0054 0.0021 0.0026 0.0023 0.0011 0.0013 0.0024 0.0017 0.0024 0.0043 0.0008 0.0005
Punctate Nuclear 0.0006 0 0.0002 0 0 0 0.0048 0 0.0003 0.0061 0.0018 0.0002 0.0021 0 0.0024 0.0026 0.0005 0.0018
Vacuole 0.0044 0.0026 0.0025 0.0023 0.0124 0.0003 0.0026 0.0017 0.0053 0.0016 0.0045 0.0004 0.0041 0.0056 0.0046 0.0031 0.0001 0.0004
Vacuole Periphery 0.0052 0.0088 0.0131 0.0102 0.0302 0.0052 0.0086 0.0078 0.0039 0.0044 0.0044 0.0062 0.01 0.0106 0.0115 0.0041 0.0007 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.987 57.9005 56.2632 67.1612 61.9832 77.0645 67.4291 64.9137 70.9608 72.5185
Translational Efficiency 1.8692 1.6884 1.8273 1.541 1.5062 1.2727 1.2885 1.2817 1.23 1.2582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1529 856 568 1473 400 2287 492 156 1929 3143 1060 1629

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1005.13 1381.70 1587.00 1441.99 1246.89 1327.01 1376.19 1452.29 1055.26 1341.90 1489.15 1442.98
Standard Deviation 164.70 276.39 312.22 253.86 211.26 271.28 275.32 231.02 200.91 273.77 313.80 251.78
Intensity Change Log 2 — 0.459062 0.658920 0.520679 — 0.089845 0.142345 0.219995 — 0.266386 0.395931 0.361985

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001006 0.002449 0.002684 0.002736 0.000870 0.000620 0.002897 0.002986 0.000978 0.001118 0.002783 0.002760
Bud Neck 0.000629 0.001283 0.004725 0.001762 0.001942 0.000530 0.013415 0.006611 0.000901 0.000735 0.008759 0.002227
Bud Site 0.001564 0.001483 0.004111 0.007197 0.001241 0.000607 0.018532 0.011878 0.001497 0.000846 0.010805 0.007645
Cell Periphery 0.000317 0.000163 0.000143 0.000682 0.000233 0.000134 0.000540 0.000347 0.000300 0.000142 0.000327 0.000650
Cytoplasm 0.000228 0.000288 0.000641 0.000664 0.000209 0.000109 0.001906 0.000713 0.000224 0.000158 0.001228 0.000669
Cytoplasmic Foci 0.009922 0.014600 0.038487 0.008897 0.023872 0.006076 0.029058 0.013654 0.012815 0.008398 0.034111 0.009352
Eisosomes 0.000101 0.000276 0.000260 0.000120 0.000205 0.000150 0.000188 0.000153 0.000123 0.000184 0.000227 0.000124
Endoplasmic Reticulum 0.000229 0.000170 0.000213 0.001215 0.000178 0.000066 0.000712 0.000308 0.000219 0.000094 0.000445 0.001128
Endosome 0.005316 0.004975 0.006412 0.007789 0.007338 0.002748 0.023511 0.015345 0.005735 0.003354 0.014349 0.008513
Golgi 0.012507 0.023346 0.039582 0.061306 0.016936 0.010331 0.031019 0.054481 0.013425 0.013876 0.035608 0.060653
Lipid Particles 0.005282 0.003783 0.008168 0.002413 0.007617 0.001895 0.009042 0.000511 0.005766 0.002409 0.008574 0.002230
Mitochondria 0.936393* 0.919523* 0.803936* 0.825474* 0.902171* 0.956922* 0.746893* 0.816008* 0.929296* 0.946737* 0.777460* 0.824568*
Mitotic Spindle 0.000938 0.001166 0.009139 0.006135 0.003629 0.000252 0.016057 0.006444 0.001496 0.000501 0.012350 0.006165
None 0.000972 0.001479 0.002905 0.001742 0.001788 0.001289 0.001495 0.001107 0.001141 0.001341 0.002251 0.001681
Nuclear Periphery 0.000203 0.000188 0.004523 0.000912 0.001348 0.000212 0.004957 0.000342 0.000441 0.000205 0.004724 0.000858
Nuclear Periphery Foci 0.000251 0.000256 0.001948 0.001016 0.001079 0.000225 0.004984 0.000301 0.000423 0.000233 0.003357 0.000948
Nucleolus 0.000286 0.000976 0.002189 0.000393 0.000991 0.000309 0.004132 0.000344 0.000432 0.000491 0.003091 0.000389
Nucleus 0.000124 0.000150 0.000274 0.000136 0.000221 0.000071 0.001460 0.000137 0.000144 0.000093 0.000824 0.000136
Peroxisomes 0.006843 0.009370 0.011843 0.002085 0.009046 0.002831 0.018089 0.008137 0.007300 0.004612 0.014742 0.002665
Vacuole 0.008030 0.007315 0.019718 0.019996 0.008605 0.007284 0.034546 0.033602 0.008150 0.007292 0.026600 0.021299
Vacuole Periphery 0.008859 0.006760 0.038097 0.047329 0.010482 0.007338 0.036565 0.026592 0.009195 0.007181 0.037386 0.045343

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.07 -3.64 -3.56 -0.41 -0.13 0.80 -2.97 -2.26 -2.55 -0.06 -0.45 -4.35 -4.07 -3.65 0.02
Bud Neck -2.06 -6.61 -3.81 -1.18 4.39 2.92 -6.20 -1.65 -2.16 2.01 0.94 -8.63 -3.39 -3.79 6.68
Bud Site 0.10 -2.29 -4.38 -4.52 -1.98 1.28 -5.50 -2.36 -2.51 1.20 1.31 -5.78 -5.05 -5.95 1.67
Cell Periphery 1.69 2.50 -2.46 -3.54 -4.01 0.63 -1.82 -0.70 -3.62 2.20 2.37 -0.43 -2.60 -4.14 -2.56
Cytoplasm -0.63 -1.82 -3.34 -2.81 -0.03 3.03 -1.94 -2.70 -3.27 1.41 1.17 -2.33 -3.83 -4.80 1.38
Cytoplasmic Foci -2.43 -7.72 0.70 3.11 8.10 5.12 -1.16 2.02 -2.02 3.20 3.41 -8.09 2.38 -0.89 9.70
Eisosomes -7.18 -6.72 -1.24 5.86 5.40 1.65 0.45 1.37 -0.14 1.40 -4.58 -6.26 -0.07 3.87 5.59
Endoplasmic Reticulum 1.21 0.38 -3.15 -3.33 -3.20 3.45 -4.83 -1.84 -3.83 3.30 4.23 -3.85 -3.21 -3.67 -2.38
Endosome 0.33 -1.15 -2.60 -2.58 -1.27 2.38 -5.01 -1.95 -3.46 1.83 3.38 -5.98 -2.85 -6.23 3.86
Golgi -3.43 -6.16 -11.65 -7.67 -3.74 2.07 -2.71 -3.33 -4.05 -2.01 -0.28 -6.91 -11.98 -11.94 -5.21
Lipid Particles 1.25 -1.99 2.73 1.48 4.66 3.10 -0.56 3.89 4.56 4.67 3.86 -2.13 3.76 0.30 5.58
Mitochondria 2.19 10.88 13.38 9.74 -1.61 -5.29 8.61 3.46 6.12 -2.55 -3.68 15.01 13.29 16.63 -4.11
Mitotic Spindle -0.49 -5.30 -4.83 -4.57 1.65 2.22 -3.66 -1.06 -2.91 2.61 2.44 -6.48 -4.51 -5.90 3.28
None -2.98 -6.91 -7.77 -1.44 4.07 2.56 1.28 3.04 1.38 2.15 -2.40 -6.47 -5.65 -3.61 3.21
Nuclear Periphery 0.30 -3.32 -4.64 -4.72 2.75 1.92 -2.82 1.67 -1.02 4.05 1.78 -4.86 -2.27 -4.61 4.37
Nuclear Periphery Foci -0.08 -3.15 -1.53 -1.52 1.25 2.82 -2.80 2.54 -0.87 3.43 2.32 -4.19 -1.16 -1.58 2.89
Nucleolus -1.32 -3.50 -0.96 1.10 3.26 1.49 -2.50 1.38 -0.32 3.18 -0.32 -4.20 0.31 0.58 4.28
Nucleus -0.40 -2.72 -0.31 0.21 2.51 2.34 -2.83 1.11 -1.50 3.04 1.53 -3.32 0.20 -1.32 3.36
Peroxisomes -1.34 -2.22 4.28 4.49 4.78 3.60 -2.86 0.26 -1.63 2.40 2.52 -3.97 4.60 2.61 6.97
Vacuole 0.39 -5.64 -6.46 -5.82 -0.11 0.82 -7.14 -3.15 -3.35 0.10 0.77 -9.07 -7.28 -7.99 2.15
Vacuole Periphery 1.59 -7.46 -13.93 -14.20 -1.98 1.25 -5.44 -2.30 -2.92 1.28 2.06 -9.64 -13.88 -14.99 -2.13
Standard name
Human Ortholog
Description Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tim50

Tim50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available