Standard name
Description Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.09 0 0 0 0 0 0 0 0 0.05 0 0.23 0.27 0.30 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.98 0.99 0.98 0.98 0.97 0.97 0.95 0.94 0.91 0.93 0.84 0.81 0.73 0.98 0.99 0.97 0.95 0.96 0.93 0.95 0.95 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.10 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 3 1 1 2 0 2 4 0 1 0 1 1 2 1 0 0 0 1 0 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 32 2 6 3 5 7 3 5 8 11 8 46 79 85 1 0 0 0 3 0 0 0 0
Cytoplasm 258 358 294 247 254 266 320 285 207 252 184 210 166 239 205 120 83 66 235 417 268 141 234 196
Endoplasmic Reticulum 1 0 2 2 0 0 5 3 6 11 11 7 0 0 1 5 8 6 0 1 4 1 0 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2 0 2 1 0 2 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 2 0 0 0 2 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 0 0 0 1 0 0 0 0 0 2 0 0 0 0 1 2 1 2 3
Unique Cell Count 260 373 300 250 258 272 329 294 217 269 202 226 197 295 280 122 84 68 248 435 288 149 246 221
Labelled Cell Count 262 393 302 257 262 273 334 296 219 274 206 228 213 320 295 128 92 73 248 435 288 149 246 221

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 245.8 430.5 253.4 327.2 299.2 261.0 232.1 209.2 213.3 197.3 201.4 196.6 514.6 503.4 513.2 235.7 293.7 293.8 303.0 323.5 329.5
Std Deviation (1e-4) 69.5 101.2 75.0 96.6 83.1 76.5 63.5 67.7 72.0 61.4 63.9 61.5 127.0 117.3 133.3 103.6 114.6 86.0 92.1 94.6 110.4
Intensity Change (Log2) 0.4 0.2 0.0 -0.1 -0.3 -0.2 -0.4 -0.3 -0.4 1.0 1.0 1.0 -0.1 0.2 0.2 0.3 0.4 0.4

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 3.3 0 8.4 9.3 10.0 0 0 0
Cytoplasm 0 0 0 0.8 1.4 0 2.3 0 0 0 0.9 1.3 4.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 2.7 3.3 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0