Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.31 0.33 0.34 0.23 0.15 0.08 0 – 0 0 0 0.18 0.22 0.13 – – – 0.09 0.09 0 0.09 0.08 0.09
Bud 0.05 0.1 0.06 0.11 0.09 0.15 0.11 0.07 – 0.08 0.06 0.06 0 0.05 0 – – – 0 0 0 0.05 0.08 0.07
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.05 0 0 0 0.05 0.07
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.58 0.66 0.66 0.65 0.38 0.24 0.17 0.09 – 0.05 0.05 0 0.64 0.7 0.73 – – – 0.59 0.71 0.76 0.58 0.57 0.53
Cytoplasm 0.36 0.28 0.24 0.3 0.5 0.53 0.6 0.67 – 0.66 0.64 0.6 0.35 0.28 0.29 – – – 0 0 0 0.06 0 0.05
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.11 0.06 0.05 0.09 0.09 0.1
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.08 0.08 0.08 0.08 0.27 0.3 0.28 – 0.41 0.49 0.48 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.06 0 0.06 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 120 140 91 63 62 39 27 16 – 8 5 6 44 47 37 – – – 54 47 12 39 29 35
Bud 29 44 16 21 25 39 37 29 – 30 18 20 6 11 5 – – – 20 14 3 23 28 28
Bud Neck 1 0 0 0 0 0 0 1 – 0 0 1 1 0 1 – – – 29 17 4 20 17 29
Bud Site 2 4 1 0 1 7 2 6 – 2 4 4 0 3 0 – – – – – – – – –
Cell Periphery 309 300 179 122 101 63 58 40 – 19 15 11 156 151 206 – – – 366 371 230 269 213 218
Cytoplasm 192 128 65 57 133 141 206 296 – 239 189 198 84 61 81 – – – 18 13 6 27 15 19
Endoplasmic Reticulum 22 16 13 3 5 1 3 1 – 0 0 1 5 6 11 – – – 66 30 16 40 34 41
Endosome 3 0 1 0 1 1 0 2 – 0 0 0 0 0 0 – – – 3 4 0 0 1 1
Golgi 2 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 2 1 0 1 1 2
Mitochondria 17 38 23 15 20 72 102 125 – 148 144 159 4 5 5 – – – 13 7 4 16 12 9
Nucleus 3 3 1 0 1 1 2 3 – 2 0 2 5 1 2 – – – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 2 3 – 2 1 0 0 0 0 – – – 1 0 0 0 0 0
Nucleolus 1 0 0 0 1 0 0 3 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 3 0 4 9 6 3 3 3 – 0 1 2 0 2 0 – – – 0 0 0 0 0 1
SpindlePole 13 10 10 5 2 1 5 10 – 7 5 17 9 8 5 – – – 0 0 0 0 1 2
Vac/Vac Membrane 10 1 3 3 7 3 7 8 – 3 4 1 0 0 3 – – – 38 12 19 17 16 13
Unique Cell Count 537 456 273 188 264 265 341 440 361 295 331 242 216 283 620 524 301 461 377 410
Labelled Cell Count 728 685 407 298 365 372 454 546 460 386 422 314 295 356 620 524 301 461 377 410


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.9 8.4 7.6 6.1 4.9 4.3 4.4 – 3.2 3.3 3.1 8.8 9.5 9.8 – – – 8.7 8.8 8.3
Std Deviation (1e-4) 2.4 3.2 2.5 2.4 2.2 2.4 1.7 1.8 – 1.0 1.2 1.2 3.3 2.7 2.8 – – – 6.7 5.5 4.3
Intensity Change (Log2) – – – -0.13 -0.46 -0.76 -0.96 -0.91 – -1.41 -1.36 -1.43 0.08 0.18 0.23 – – – 0.05 0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.5 -5.0 0 0 – 0 0 0 -3.9 -2.8 -5.7 – – –
Bud 2.1 1.6 3.4 0 0 – 0 0 0 -1.9 -0.4 -2.5 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery -0.1 -6.3 -9.7 0 0 – 0 0 0 -0.3 1.0 1.8 – – –
Cytoplasm 1.6 6.4 7.0 9.1 11.3 – 10.6 9.6 8.9 2.7 1.1 1.3 – – –
Endoplasmic Reticulum -1.8 -1.8 -3.2 0 0 – 0 0 0 -1.7 -1.1 -0.5 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria -0.2 -0.4 5.7 0 0 – 0 0 0 -3.4 -2.9 -3.6 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 2.1 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole -0.6 -2.3 -2.7 0 0 – 0 0 0 0 0 -1.4 – – –
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0759 0.0042 0.0176 0.0284 0.0001 0.0125 0.0529 0.0208 0.0104 0.0504 0.0204 0.0046 – – – – – –
Bud 0.0034 0.0018 0.0042 0.0038 0.0006 0.0014 0.015 0.0006 0.0022 0.0071 0.0033 0.0021 – – – – – –
Bud Neck 0.0047 0.0021 0.0275 0.0432 0.0024 0.0241 0.0032 0.0037 0.0227 0.0042 0.0068 0.0123 – – – – – –
Bud Periphery 0.0336 0.0073 0.0215 0.0454 0.014 0.0248 0.0237 0.0042 0.0143 0.0332 0.0469 0.0303 – – – – – –
Bud Site 0.0258 0.0821 0.0541 0.0444 0.0011 0.0058 0.0169 0.151 0.0817 0.0575 0.0108 0.0037 – – – – – –
Cell Periphery 0.6536 0.6273 0.6418 0.6276 0.9193 0.7668 0.5076 0.6066 0.5352 0.4859 0.671 0.6194 – – – – – –
Cytoplasm 0.0386 0.2227 0.1563 0.169 0.0336 0.0941 0.1314 0.1675 0.2434 0.1549 0.1151 0.2246 – – – – – –
Cytoplasmic Foci 0.002 0.0069 0.0086 0.0012 0 0.0004 0.0271 0.0004 0.0055 0.0293 0.0373 0.0105 – – – – – –
Eisosomes 0.0094 0.0011 0.0013 0.0027 0.0002 0.0009 0.0022 0.0006 0.0035 0.0008 0.0006 0.0007 – – – – – –
Endoplasmic Reticulum 0.0368 0.0134 0.01 0.0101 0.0125 0.0189 0.0142 0.0083 0.0123 0.0071 0.0077 0.0037 – – – – – –
Endosome 0.0021 0.0006 0.0043 0.0003 0 0.0108 0.014 0 0.0006 0.0033 0.0055 0.0017 – – – – – –
Golgi 0.0032 0.0006 0.0039 0.001 0.0001 0.0009 0.0094 0.0001 0.0006 0.0059 0.0034 0.0017 – – – – – –
Lipid Particles 0.0052 0.0042 0.0031 0.0016 0.0012 0.0056 0.0333 0.0015 0.0029 0.0114 0.0116 0.0018 – – – – – –
Mitochondria 0.0034 0.0006 0.0023 0.0002 0 0.0001 0.0064 0 0.0046 0.002 0.0005 0.0044 – – – – – –
None 0.037 0.0081 0.0039 0.0005 0.0001 0.0003 0.0725 0.0278 0.0423 0.0891 0.005 0.0574 – – – – – –
Nuclear Periphery 0.0117 0.0012 0.0014 0.0009 0.0008 0.0009 0.0057 0.0004 0.0008 0.0005 0.0014 0.0003 – – – – – –
Nucleolus 0.0007 0.0002 0.0004 0.0002 0 0.0001 0.0015 0.0002 0.0013 0.0018 0.0004 0.0039 – – – – – –
Nucleus 0.0277 0.0005 0.001 0.0007 0.0002 0.0003 0.0062 0.0003 0.0034 0.0017 0.0006 0.002 – – – – – –
Peroxisomes 0.0002 0.0004 0.0149 0.0001 0 0 0.0244 0 0.0001 0.0176 0.0239 0.0004 – – – – – –
Punctate Nuclear 0.0003 0.0002 0.0006 0.0001 0 0 0.0075 0 0.0008 0.0037 0.0191 0.0003 – – – – – –
Vacuole 0.0235 0.0145 0.0211 0.0182 0.0135 0.0307 0.0232 0.0057 0.0111 0.0322 0.0086 0.0135 – – – – – –
Vacuole Periphery 0.001 0.0001 0.0004 0.0004 0.0001 0.0004 0.0018 0 0.0003 0.0003 0.0002 0.0007 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.827 92.2009 28.7223 24.2255 19.5542 57.3764 84.2097 45.8545 25.7173 16.7478
Translational Efficiency 0.5538 0.6705 0.5419 0.5232 0.4295 0.693 0.6052 0.395 0.3238 0.2165

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
349 1352 224 971 1910 2095 444 1825 2259 3447 668 2796

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 898.18 1088.59 1972.06 1811.41 674.98 1113.03 2905.40 2039.87 709.46 1103.44 2592.42 1960.53
Standard Deviation 183.41 249.69 422.04 517.00 96.89 224.14 579.25 444.56 140.15 234.80 690.59 483.38
Intensity Change Log 2 — 0.277384 1.134627 1.012037 — 0.721576 2.105820 1.595561 — 0.484900 1.632465 1.291673

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.022897 0.004149 0.006607 0.003926 0.012017 0.002082 0.000710 0.001536 0.013698 0.002892 0.002687 0.002366
Bud Neck 0.061698 0.009652 0.029505 0.002147 0.113430 0.006271 0.001498 0.001115 0.105438 0.007597 0.010889 0.001474
Bud Site 0.164921* 0.091829 0.102639* 0.027007 0.104851* 0.034300 0.004809 0.011615 0.114131* 0.056864 0.037614 0.016961
Cell Periphery 0.359254* 0.780888* 0.554664* 0.939006* 0.073139 0.922149* 0.957691* 0.970971* 0.117342* 0.866743* 0.822544* 0.959871*
Cytoplasm 0.210449* 0.080648 0.064202 0.006214 0.471662* 0.025691 0.000222 0.003400 0.431306* 0.047246 0.021677 0.004378
Cytoplasmic Foci 0.012065 0.005499 0.015488 0.000330 0.057064 0.000692 0.000054 0.000162 0.050112 0.002578 0.005230 0.000220
Eisosomes 0.018393 0.000855 0.006628 0.002282 0.028646 0.001246 0.007794 0.001033 0.027062 0.001093 0.007403 0.001467
Endoplasmic Reticulum 0.010847 0.007633 0.020107 0.002373 0.004488 0.002343 0.000632 0.001509 0.005471 0.004418 0.007163 0.001809
Endosome 0.007990 0.003182 0.012187 0.001272 0.008364 0.000446 0.000198 0.000391 0.008306 0.001519 0.004218 0.000697
Golgi 0.006403 0.002889 0.005620 0.001515 0.010311 0.000255 0.000452 0.000664 0.009707 0.001288 0.002185 0.000959
Lipid Particles 0.006124 0.002287 0.058474 0.001262 0.011178 0.000228 0.000283 0.000729 0.010397 0.001036 0.019796 0.000914
Mitochondria 0.005823 0.002266 0.000082 0.000740 0.007717 0.001300 0.000171 0.000490 0.007425 0.001679 0.000141 0.000577
Mitotic Spindle 0.000788 0.000285 0.000918 0.000542 0.000084 0.000220 0.000541 0.000257 0.000192 0.000246 0.000668 0.000356
None 0.000051 0.000099 0.000035 0.000026 0.003930 0.000039 0.000015 0.000026 0.003331 0.000063 0.000022 0.000026
Nuclear Periphery 0.000153 0.000124 0.002341 0.000802 0.000416 0.000103 0.001233 0.000415 0.000375 0.000111 0.001605 0.000550
Nuclear Periphery Foci 0.000982 0.000397 0.003481 0.000167 0.000386 0.000083 0.000087 0.000103 0.000478 0.000206 0.001225 0.000125
Nucleolus 0.000056 0.000072 0.000035 0.000037 0.000321 0.000018 0.000052 0.000038 0.000280 0.000039 0.000046 0.000038
Nucleus 0.015132 0.000389 0.000650 0.000324 0.026957 0.000260 0.000130 0.000133 0.025130 0.000311 0.000304 0.000200
Peroxisomes 0.001597 0.001165 0.000770 0.000334 0.007913 0.000579 0.000008 0.000042 0.006938 0.000809 0.000263 0.000144
Vacuole 0.089455 0.004366 0.114112 0.008155 0.056140 0.001319 0.022055 0.004021 0.061287 0.002515 0.052924 0.005457
Vacuole Periphery 0.004923 0.001326 0.001454 0.001538 0.000986 0.000376 0.001364 0.001348 0.001594 0.000749 0.001395 0.001414

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.45 4.57 5.30 0.16 1.80 11.74* 13.39 11.97 1.19 -1.83 12.11* 11.59 11.72 1.02 0.53
Bud Neck 9.41 4.46 10.88 8.36 5.85 29.90* 31.47* 31.62* 10.23 1.54 30.82 26.62 33.05 13.15 5.68
Bud Site 5.44 3.48 10.73 10.61 5.83 15.19* 23.25* 22.06* 7.84 -2.99 12.37 12.49 23.31 13.43 4.17
Cell Periphery -20.67* -6.45 -30.30* -15.18 -15.63 -153.10* -171.17* -203.20* -10.35 -3.10 -114.83 -57.96 -157.41 -18.37 -12.02
Cytoplasm 10.28 9.36 17.29 15.23 5.64 73.79* 82.97* 82.07* 10.48 -5.88 64.50* 62.08 77.14 18.27 4.74
Cytoplasmic Foci 2.74 -0.66 5.23 5.31 3.26 23.43 23.83 23.78 2.00 -3.06 22.43 17.09 23.98 5.67 3.17
Eisosomes 8.69 4.60 7.93 -5.69 2.73 20.36* 11.94 20.56 1.23 6.02 22.01 13.21 21.66 -2.68 6.47
Endoplasmic Reticulum 1.36 -2.01 3.93 4.60 4.33 3.30 6.38 4.31 1.78 -2.20 1.40 -1.10 5.58 4.88 3.71
Endosome 2.17 -1.20 3.02 2.17 3.81 15.52 16.11 15.49 0.38 -2.17 11.47 3.72 13.01 2.43 3.56
Golgi 1.58 0.32 2.14 1.45 3.12 18.14* 17.72 16.25 -1.87 -0.97 13.73 11.01 13.53 0.85 2.53
Lipid Particles 1.89 -5.12 2.29 0.92 5.67 13.97 13.27 12.33 -1.52 -1.08 12.16 -2.61 11.61 0.25 5.32
Mitochondria 1.07 1.76 1.55 2.26 -2.70 10.64 17.19 15.90 1.87 -2.63 8.03 11.68 10.79 2.97 -3.76
Mitotic Spindle 1.24 -0.31 0.60 -4.66 2.69 -11.76 -12.54 -12.28 -2.14 7.30 -0.85 -5.93 -2.50 -4.91 5.74
None -1.90 1.34 2.58 3.09 0.98 4.91 4.94 4.92 1.02 -1.51 4.87 4.93 4.92 3.00 -0.68
Nuclear Periphery 0.56 -4.65 -7.15 -8.92 3.25 1.07 -2.47 0.00 -9.09 5.18 1.07 -3.95 -0.70 -12.57 5.52
Nuclear Periphery Foci 3.48 -2.80 6.16 2.10 3.75 2.82 2.78 2.60 -1.62 -1.21 2.67 -2.36 3.75 1.81 3.64
Nucleolus -0.48 1.03 1.08 1.29 -0.22 13.20* 11.64 12.29 -10.75 4.52 10.77 11.59 12.26 0.13 1.85
Nucleus 5.06 4.97 5.08 0.83 2.92 23.53 23.66 23.66 2.59 -0.18 23.26 23.27 23.38 2.67 2.53
Peroxisomes 1.09 2.19 2.94 2.70 1.53 16.89* 19.18 19.10 3.91 -3.63 16.46 18.58 18.55 4.76 1.21
Vacuole 8.75 -1.78 8.35 -4.11 11.41 19.77 9.67 18.73 -7.09 8.12 20.97 1.81 19.88 -7.01 12.39
Vacuole Periphery 1.67 1.61 1.56 -0.83 -0.37 3.95 -3.77 -2.96 -5.54 0.06 2.38 0.57 0.48 -4.46 -0.25