Standard name
Human Ortholog
Description Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.06 0.12 0.12 0.13 0.16 0.14 – 0.19 0.29 0.23 0.11 0.23 0.37 0.05 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.13 0.14 0 0 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.12 0 0.09 0.07 0.07 0.05 – 0.06 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.21 0.13 0.2 0.24 0.08 0.24 0.23 0.19 – 0.13 0.17 0.13 0.08 0.06 0 0.53 0.43 0.35 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.7 0.86 0.77 0.77 0.83 0.72 0.7 0.77 – 0.76 0.69 0.72 0.86 0.75 0.65 0.55 0.64 0.65 0.84 0.9 0.89 0.86 0.86 0.86
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 – 0 0 1 0 1 2 0 0 0 0 1 0 0 1 0
Bud 0 0 0 0 2 0 0 3 – 0 2 3 0 0 0 0 0 0 4 3 2 2 2 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 10 17 18 46 85 106 159 175 – 185 218 246 39 99 166 15 7 20 1 1 1 2 1 1
Endoplasmic Reticulum 0 1 0 0 2 1 10 4 – 2 0 3 0 3 0 15 9 6 0 0 1 1 1 2
Endosome 14 8 5 2 21 2 5 8 – 4 2 5 23 56 65 6 6 7 17 7 11 12 18 24
Golgi 8 2 4 0 3 0 1 0 – 0 0 0 12 8 2 0 2 2 9 1 4 4 4 5
Mitochondria 50 10 40 12 61 55 67 58 – 57 100 102 4 3 2 5 6 2 6 0 4 5 7 5
Nucleus 82 32 64 93 59 188 233 232 – 130 129 136 28 24 16 166 109 83 18 4 5 5 6 4
Nuclear Periphery 6 0 4 3 6 4 10 3 – 5 3 15 0 1 0 0 1 1 7 1 2 1 3 4
Nucleolus 20 1 2 2 5 1 10 3 – 6 1 8 1 1 2 2 1 3 9 0 0 0 2 1
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 – 0 0 0 0 0 5 0 0 2 2 1 0 2 2 0
Vac/Vac Membrane 278 219 247 292 590 566 715 932 – 750 519 768 295 321 296 173 161 157 404 240 298 255 323 358
Unique Cell Count 400 254 321 381 708 789 1023 1214 989 755 1071 343 426 452 315 253 240 484 266 336 298 376 418
Labelled Cell Count 468 290 384 450 834 924 1210 1419 1140 975 1287 402 517 557 383 303 283 484 266 336 298 376 418


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 11.6 8.9 9.3 10.3 9.9 9.5 9.7 – 9.6 8.6 9.3 9.6 8.2 7.4 13.8 13.2 14.0 11.9 13.1 13.2
Std Deviation (1e-4) 4.5 4.6 3.4 3.3 3.6 4.0 3.8 3.6 – 3.6 3.5 3.3 3.4 2.6 2.1 5.3 5.9 5.5 4.1 4.4 4.2
Intensity Change (Log2) – – – 0.07 0.21 0.16 0.09 0.12 – 0.12 -0.05 0.06 0.12 -0.12 -0.26 0.64 0.57 0.66 0.42 0.56 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 3.2 3.8 4.6 4.2 – 5.6 8.4 7.0 2.6 6.6 10.0 -0.5 -1.7 1.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 4.0 0 0
Endosome 0 1.3 0 0 0 – 0 0 0 3.3 5.7 6.1 0.3 0 1.1
Golgi 0 0 0 0 0 – 0 0 0 1.9 0.7 0 0 0 0
Mitochondria -4.7 -1.9 -3.0 -3.4 -5.0 – -4.0 0.3 -1.5 -5.8 -6.8 -7.3 -5.3 -4.4 -5.2
Nucleus 1.4 -5.3 1.4 1.1 -0.3 – -3.0 -1.1 -3.2 -4.4 -6.0 -7.4 8.6 6.0 3.9
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -0.1 2.4 -1.8 -2.4 -0.1 – -0.4 -2.7 -1.9 3.0 -0.5 -3.4 -5.9 -3.5 -3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8707 5.0718 4.6876 3.8873 3.3858 4.4756 5.3353 10.0148 7.8611 7.7512 7.4862 8.462 2.8887 5.8233 5.266 5.251 4.7443 5.1881
Actin 0.0141 0 0.0006 0.0001 0.0036 0.0008 0.0054 0 0.0024 0 0 0 0.016 0 0.0048 0.0001 0.0062 0.0001
Bud 0.0024 0.0031 0.0006 0.0002 0.0002 0.0005 0.0003 0.0003 0.0003 0.0001 0.0002 0.0003 0.0004 0.0004 0.0003 0.0004 0.0003 0.0002
Bud Neck 0.0011 0.0015 0.0008 0.0007 0.0009 0.0014 0.0006 0.0006 0.0007 0.0003 0.0007 0.0007 0.0033 0.0008 0.0015 0.0008 0.0008 0.0007
Bud Periphery 0.0017 0.0015 0.0009 0.0004 0.0005 0.0012 0.001 0.0007 0.0008 0.0002 0.0006 0.001 0.0011 0.0008 0.0008 0.0008 0.0007 0.0008
Bud Site 0.0029 0.0037 0.0022 0.0004 0.0007 0.0006 0.0007 0.0004 0.0008 0.0001 0.0008 0.0004 0.0017 0.001 0.0008 0.0011 0.0004 0.0004
Cell Periphery 0.0017 0.0025 0.0012 0.002 0.0009 0.003 0.0017 0.0023 0.0032 0.0016 0.0024 0.0026 0.0012 0.0025 0.0015 0.0014 0.0015 0.0017
Cytoplasm 0.0101 0.0061 0.0035 0.0012 0.0014 0.0015 0.002 0.0036 0.0016 0.0005 0.0006 0.0012 0.0059 0.0029 0.0019 0.0019 0.0037 0.0011
Cytoplasmic Foci 0.0241 0.0209 0.0241 0.0192 0.0029 0.0127 0.0062 0.0018 0.0049 0.0009 0.0007 0.0014 0.0089 0.0063 0.0078 0.0036 0.0061 0.0022
Eisosomes 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0033 0.0023 0.0027 0.0008 0.0019 0.0022 0.0025 0.0031 0.0027 0.0003 0.0047 0.0022 0.0067 0.0045 0.0031 0.0018 0.0031 0.0018
Endosome 0.3057 0.154 0.2428 0.1593 0.1151 0.1041 0.1843 0.0948 0.1577 0.0669 0.0787 0.1078 0.2007 0.1176 0.2036 0.1343 0.1719 0.1384
Golgi 0.0198 0.01 0.0204 0.0234 0.0188 0.0113 0.0091 0.0079 0.0095 0.0008 0.0015 0.0043 0.0098 0.0034 0.0152 0.0029 0.0072 0.0058
Lipid Particles 0.0524 0.008 0.0093 0.0187 0.01 0.0099 0.0208 0.0024 0.0136 0.0033 0.0032 0.005 0.027 0.0066 0.0145 0.0021 0.006 0.0089
Mitochondria 0.043 0.0084 0.0333 0.0058 0.0114 0.0144 0.0218 0.0052 0.0227 0.0003 0.0098 0.0043 0.0298 0.0086 0.0274 0.0019 0.003 0.0163
None 0.0038 0.001 0.0003 0.0001 0.0001 0.0002 0.0007 0.0001 0.0001 0 0 0.0001 0.005 0.0006 0.0003 0.0001 0.0013 0.0001
Nuclear Periphery 0.0159 0.0049 0.0041 0.0023 0.0046 0.005 0.0063 0.0091 0.0043 0.0008 0.0025 0.0042 0.0175 0.0106 0.0113 0.0023 0.0069 0.0121
Nucleolus 0.0115 0.0085 0.0066 0.0058 0.0013 0.0083 0.0075 0.0008 0.0052 0.0008 0.0033 0.0015 0.0042 0.005 0.0024 0.002 0.0015 0.002
Nucleus 0.0057 0.0039 0.0029 0.002 0.0052 0.0043 0.0054 0.0031 0.0022 0.0011 0.0035 0.0028 0.0049 0.0034 0.003 0.0025 0.0025 0.0038
Peroxisomes 0.0058 0.0011 0.0024 0.0011 0.0011 0.0028 0.0017 0.0001 0.0007 0.0002 0.0003 0.0003 0.0068 0.0004 0.0022 0.0002 0.0007 0.0005
Punctate Nuclear 0.0042 0.0003 0.0004 0.0004 0.0001 0.0001 0.0014 0 0.0001 0 0 0 0.0039 0.0001 0.0003 0.0001 0.0011 0
Vacuole 0.3273 0.5695 0.4322 0.6114 0.641 0.612 0.4626 0.6118 0.5065 0.702 0.6626 0.5978 0.4291 0.6159 0.4603 0.666 0.5969 0.555
Vacuole Periphery 0.1435 0.1888 0.2085 0.1447 0.1783 0.2038 0.2578 0.2516 0.26 0.2199 0.2237 0.2619 0.2156 0.2086 0.237 0.1739 0.1781 0.2481

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.6547 24.037 16.5625 58.1932 34.2401 25.9142 29.7288 21.0901 40.9775 52.9443
Translational Efficiency 0.7464 0.7013 0.7561 0.6441 0.8646 1.3428 0.5556 0.4787 0.7274 0.5865

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘