Standard name
Human Ortholog
Description Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0.06 0.17 0.2 0.11 0.07 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.19 0.19 0.05 0.26 0.33 0.45 0.43 0.36 0.54 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.91 0.93 0.93 0.86 0.85 0.77 0.73 0.72 0.69 0.74 0.68 0.88 0.79 0.77 0.79 0.78 0.71 0.91 0.89 0.86 0.71 0.69 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.1 0.13 0.16 0.11 0.25 0.23 0.25 0.25 0.27 0.22 0.13 0.08 0.08 0 0.08 0.09 0 0 0.07 0.19 0.21 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 2 1 3 3 0 0 0 0 0 0 0 0 3 1 0 1 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 1 1 0 6 8 6 4 7 3 1 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 6 10 12 4 8 7 8 5 4 17 47 67 14 9 18 0 0 0 0 0 1
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 1 0 1 2 1 1 0 0 1 0 0 0
Endosome 0 2 0 0 1 0 0 0 0 1 0 1 1 2 2 1 2 1 4 1 0 4 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 4 0 1 1 0 1 0
Mitochondria 14 7 60 35 13 66 140 131 109 67 96 4 0 1 3 4 9 1 2 2 1 0 4
Nucleus 215 389 291 161 209 198 313 211 173 140 120 237 224 259 102 94 108 224 384 279 147 163 172
Nuclear Periphery 0 1 0 1 0 1 3 1 2 0 1 0 1 1 0 0 0 0 0 0 0 0 1
Nucleolus 29 40 41 29 28 64 98 73 63 51 39 35 23 27 4 10 13 8 19 21 39 49 50
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 2 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 0 1 2 0 1 2 4 0 0 1 1 1 2 3 1 6
Vac/Vac Membrane 0 5 1 0 2 4 6 9 3 2 1 0 2 1 3 5 7 6 8 6 9 10 31
Unique Cell Count 237 418 314 187 246 257 429 294 252 188 177 269 282 338 129 120 152 247 431 324 207 236 288
Labelled Cell Count 260 451 402 238 265 344 579 439 366 278 264 299 303 368 133 127 163 247 431 324 207 236 288


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 9.8 6.0 7.2 7.7 5.8 5.5 5.0 5.2 5.2 4.8 9.7 9.4 8.8 13.5 13.2 12.1 9.6 10.1 9.6
Std Deviation (1e-4) 1.3 1.7 1.5 2.0 1.9 1.2 1.3 1.5 1.6 1.5 0.9 2.6 2.8 2.6 3.7 3.0 3.2 2.1 1.9 2.4
Intensity Change (Log2) 0.26 0.36 -0.05 -0.12 -0.26 -0.22 -0.21 -0.34 0.69 0.64 0.55 1.16 1.12 1.0 0.67 0.75 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 2.0 0 0 0.4 1.0 0 0 2.7 6.3 7.2 4.1 0 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 -4.8 1.9 4.1 6.8 6.2 4.1 0 -6.8 -7.7 -8.2 -4.6 -4.1 -3.8
Nucleus -2.4 -2.9 -5.3 -6.8 -6.8 -7.4 -5.7 -7.2 -1.9 -4.7 -5.6 -4.1 -4.2 -6.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 -0.6 3.6 3.4 3.7 3.6 3.9 0 0 -1.9 -2.1 -3.2 -1.4 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3207 5.2079 5.0714 5.0925 4.7804 5.1879 3.5095 3.7287 3.617 3.6466 3.4853 3.7623 7.6011 7.328 6.986 6.4737 6.6337 6.9885
Actin 0.0029 0 0.0077 0 0 0 0.0274 0.0001 0.0004 0.0037 0.0047 0.0019 0 0 0 0 0 0
Bud 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0007 0.0001 0.0002 0.0008 0.0011 0.0001 0 0 0 0 0 0.0001
Bud Neck 0.0005 0.0006 0.0006 0.0001 0.0011 0.0006 0.0058 0.0002 0.0004 0.0012 0.003 0.0008 0.0001 0.0001 0.0001 0 0.0001 0.0004
Bud Periphery 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0019 0.0003 0.0005 0.0014 0.0015 0.0001 0 0 0 0 0 0.0002
Bud Site 0.002 0.0008 0.001 0 0.0003 0.0001 0.0059 0.0019 0.0022 0.0016 0.0036 0.0001 0.0001 0.0003 0.0001 0 0 0.0001
Cell Periphery 0.0001 0 0.0002 0 0 0 0.0007 0.0001 0.0003 0.0003 0.0003 0 0 0 0 0 0 0
Cytoplasm 0.0063 0.0048 0.0005 0.001 0.1097 0.0003 0.0075 0.0014 0.0057 0.0056 0.0132 0.0001 0.0002 0.003 0.0001 0 0 0.0001
Cytoplasmic Foci 0.0047 0.0004 0.0005 0.0103 0.0114 0.0006 0.0103 0.0012 0.0042 0.0012 0.0069 0.0007 0 0.0003 0 0 0 0
Eisosomes 0 0 0.0003 0 0 0 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0.0002 0.0007 0 0.0002 0 0.0046 0.0002 0.0012 0.0036 0.0073 0.0001 0 0.0002 0 0 0 0.0001
Endosome 0.0066 0.0009 0.0013 0 0.0112 0 0.0178 0.0014 0.0133 0.0096 0.0461 0.0035 0 0.0009 0 0 0 0.0003
Golgi 0.0013 0 0.0005 0 0.0002 0 0.004 0.0002 0.002 0.0013 0.0147 0.0044 0 0 0 0 0 0
Lipid Particles 0.0019 0.0001 0.0016 0.0001 0 0 0.0045 0.0011 0.0041 0.0017 0.0348 0.0031 0 0.0001 0 0 0 0
Mitochondria 0.0008 0.0003 0.0007 0.0001 0.0004 0.0001 0.0069 0.0005 0.0051 0.0115 0.0054 0.0029 0.0001 0.0001 0.0001 0 0 0.0002
None 0.0019 0.0075 0.0007 0.0013 0.0005 0.0001 0.003 0.0005 0.0072 0.0011 0.001 0 0 0.0002 0 0 0 0
Nuclear Periphery 0.0097 0.0051 0.0046 0.0006 0.0012 0.0004 0.0227 0.0024 0.0017 0.0147 0.0228 0.0006 0.0005 0.0029 0.0004 0.0001 0.0002 0.0019
Nucleolus 0.0267 0.0214 0.0144 0.012 0.0596 0.0432 0.0143 0.0134 0.0148 0.0098 0.0536 0.0382 0.0077 0.0123 0.0057 0.0069 0.0333 0.0196
Nucleus 0.9046 0.9314 0.9601 0.8879 0.7947 0.9462 0.8426 0.9727 0.9299 0.9071 0.7543 0.9417 0.9906 0.9675 0.9931 0.9929 0.9659 0.976
Peroxisomes 0.0009 0 0.0002 0 0.0001 0 0.0023 0.0002 0.0005 0.0004 0.0009 0.0007 0 0 0 0 0 0
Punctate Nuclear 0.0267 0.0232 0.0035 0.0865 0.0015 0.0081 0.0063 0.0006 0.0028 0.0181 0.0143 0.0008 0.0005 0.0086 0.0002 0.0001 0.0004 0.0005
Vacuole 0.0006 0.0024 0.0003 0 0.0065 0.0001 0.008 0.0013 0.0028 0.0032 0.0073 0.0002 0.0001 0.0032 0.0001 0 0 0.0003
Vacuole Periphery 0.0003 0.0004 0.0003 0 0.001 0 0.0023 0.0003 0.0007 0.0019 0.0029 0.0001 0 0.0003 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7121 23.0154 17.0785 17.0175 26.2279 11.0279 19.1154 20.1938 18.7395 19.9452
Translational Efficiency 1.3575 1.0119 0.9399 0.7584 0.6053 2.3691 1.0773 1.0726 1.3669 1.2178

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
60 1009 256 490 1453 1193 11 799 1513 2202 267 1289

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1135.40 984.99 1172.31 1060.58 854.84 941.36 485.78 1021.18 865.97 961.35 1144.03 1036.16
Standard Deviation 165.00 165.80 155.20 170.46 143.63 148.14 87.47 175.84 154.56 157.98 205.01 174.86
Intensity Change Log 2 -0.205020 0.046153 -0.098347 0.139092 -0.815351 0.256511 -0.047073 -0.263420 0.064861

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002189 0.000672 0.001425 0.002343 0.000426 0.000553 0.004031 0.001083 0.000496 0.000607 0.001532 0.001562
Bud Neck 0.014698 0.011345 0.010402 0.029740 0.007636 0.011816 0.019487 0.026538 0.007916 0.011600 0.010776 0.027755
Bud Site 0.007042 0.001072 0.001326 0.005606 0.000662 0.000959 0.037540 0.004433 0.000915 0.001011 0.002818 0.004879
Cell Periphery 0.000252 0.000097 0.000069 0.000235 0.000053 0.000082 0.019759 0.000224 0.000061 0.000089 0.000880 0.000228
Cytoplasm 0.008794 0.002919 0.000237 0.006816 0.000496 0.003573 0.062265 0.007532 0.000825 0.003274 0.002793 0.007259
Cytoplasmic Foci 0.002070 0.000104 0.000054 0.003827 0.000065 0.001524 0.004935 0.001621 0.000144 0.000873 0.000256 0.002460
Eisosomes 0.000098 0.000040 0.000075 0.000040 0.000029 0.000038 0.001456 0.000038 0.000032 0.000039 0.000132 0.000039
Endoplasmic Reticulum 0.008330 0.002793 0.004368 0.004481 0.002505 0.003366 0.015550 0.003165 0.002736 0.003103 0.004829 0.003666
Endosome 0.001664 0.000411 0.000110 0.011525 0.000169 0.000450 0.000982 0.000851 0.000228 0.000432 0.000146 0.004908
Golgi 0.000579 0.000159 0.000140 0.004572 0.000153 0.000141 0.000162 0.001848 0.000170 0.000149 0.000141 0.002883
Lipid Particles 0.000324 0.000217 0.000092 0.000494 0.000063 0.000271 0.000198 0.001167 0.000073 0.000246 0.000096 0.000912
Mitochondria 0.001488 0.000892 0.001449 0.014695 0.000737 0.001958 0.001289 0.002207 0.000766 0.001470 0.001442 0.006954
Mitotic Spindle 0.009539 0.001559 0.000898 0.043337 0.002036 0.003723 0.000157 0.022906 0.002334 0.002732 0.000868 0.030672
None 0.001732 0.003716 0.001621 0.008234 0.001973 0.006575 0.273300* 0.012155 0.001964 0.005265 0.012814 0.010665
Nuclear Periphery 0.000235 0.000230 0.000147 0.003017 0.000104 0.000567 0.000279 0.000669 0.000109 0.000413 0.000152 0.001561
Nuclear Periphery Foci 0.000226 0.000241 0.000105 0.001992 0.000253 0.000484 0.020558 0.000325 0.000252 0.000373 0.000948 0.000959
Nucleolus 0.015970 0.049738 0.038451 0.012920 0.046782 0.045728 0.007935 0.013433 0.045560 0.047566 0.037194 0.013238
Nucleus 0.914464* 0.921158* 0.936235* 0.817287* 0.935154* 0.915421* 0.510074* 0.895011* 0.934333* 0.918050* 0.918677* 0.865465*
Peroxisomes 0.001250 0.000620 0.001819 0.001021 0.000389 0.000809 0.000273 0.000345 0.000423 0.000722 0.001755 0.000602
Vacuole 0.008739 0.000919 0.000827 0.019338 0.000170 0.000945 0.019713 0.003786 0.000510 0.000933 0.001605 0.009698
Vacuole Periphery 0.000316 0.001097 0.000150 0.008479 0.000146 0.001017 0.000057 0.000662 0.000153 0.001054 0.000146 0.003633

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.95 2.69 0.38 -4.56 -2.23 NaN NaN NaN NaN NaN -2.55 -9.30 -8.21 -7.63 -0.64
Bud Neck 2.56 2.59 -3.80 -9.72 -9.85 NaN NaN NaN NaN NaN -4.59 -3.54 -15.37 -12.64 -11.49
Bud Site 1.84 1.69 -0.06 -6.95 -6.45 NaN NaN NaN NaN NaN -0.54 -2.57 -7.18 -7.37 -2.41
Cell Periphery 0.91 1.20 0.21 -2.32 -3.90 NaN NaN NaN NaN NaN -1.48 -2.60 -4.73 -3.54 2.24
Cytoplasm 0.64 1.17 0.04 -2.11 -7.00 NaN NaN NaN NaN NaN -3.12 -1.67 -6.41 -2.65 -2.40
Cytoplasmic Foci 1.00 1.04 -0.10 -2.36 -2.44 NaN NaN NaN NaN NaN -1.82 -1.44 -2.26 -0.27 -1.90
Eisosomes 6.36 3.15 5.22 -3.02 4.70 NaN NaN NaN NaN NaN -3.01 -3.73 -6.46 -4.10 3.19
Endoplasmic Reticulum 5.97 3.88 2.89 -6.88 -1.99 NaN NaN NaN NaN NaN -1.98 -2.55 -7.94 -6.76 -0.06
Endosome 0.80 1.14 -1.80 -2.58 -2.79 NaN NaN NaN NaN NaN -1.75 0.89 -3.05 -2.69 -3.21
Golgi 1.20 1.19 -2.24 -3.19 -3.19 NaN NaN NaN NaN NaN 0.47 0.21 -2.58 -2.66 -2.62
Lipid Particles 0.70 2.48 -1.07 -1.50 -4.34 NaN NaN NaN NaN NaN -1.97 -1.11 -1.67 -1.03 -1.57
Mitochondria 4.51 1.73 -2.85 -3.37 -3.07 NaN NaN NaN NaN NaN -2.55 -6.08 -4.59 -3.63 -3.68
Mitotic Spindle 0.92 0.97 -2.16 -5.48 -5.57 NaN NaN NaN NaN NaN -0.15 1.06 -7.71 -7.76 -8.22
None -0.85 0.39 -2.42 -1.90 -4.78 NaN NaN NaN NaN NaN -3.33 -2.25 -6.11 -1.94 1.03
Nuclear Periphery 1.58 2.69 -4.01 -4.32 -4.46 NaN NaN NaN NaN NaN -4.31 -2.35 -6.93 -5.42 -6.67
Nuclear Periphery Foci 0.06 1.19 -2.65 -2.71 -3.02 NaN NaN NaN NaN NaN -0.60 -1.57 -2.66 -2.31 -0.37
Nucleolus -4.11 -2.66 1.08 10.69 5.25 NaN NaN NaN NaN NaN -0.75 0.76 13.69 15.27 5.39
Nucleus -0.97 -1.30 2.44 7.11 7.35 NaN NaN NaN NaN NaN 4.13 2.57 10.01 7.46 4.26
Peroxisomes 3.09 -0.90 2.43 -0.68 3.04 NaN NaN NaN NaN NaN -1.52 -3.90 -0.83 1.03 3.70
Vacuole 1.77 1.77 -0.95 -4.61 -4.62 NaN NaN NaN NaN NaN -1.48 -2.87 -5.93 -5.64 -4.99
Vacuole Periphery -1.52 1.47 -2.39 -1.59 -2.53 NaN NaN NaN NaN NaN -2.64 -0.19 -2.81 -0.72 -2.80