Standard name
Description Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm – 0.93 0.93 0.77 0.65 0.59 0.54 0.5 0.4 0.37 0.31 0.36 0.88 0.93 0.93 0.6 0.6 0.6 – 0.89 0.77 0.83 0.83 0.83
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – 0 0 0 0 0
Endosome – 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria – 0 0.1 0.22 0.12 0.45 0.46 0.49 0.63 0.6 0.68 0.68 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus – 0 0 0 0 0 0.05 0.05 0.08 0.09 0.11 0.11 0.05 0 0 0 0 0 – 0 0 0.05 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – 0.07 0.06 0.12 0.32 0.18 0.19 0.18 0.18 0.15 0.13 0.16 0.14 0.1 0.1 0.54 0.49 0.44 – 0 0.1 0.07 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 1 0 1 3
Bud – 1 4 6 1 10 17 12 12 8 22 19 0 0 0 0 0 0 – 2 1 0 5 11
Bud Neck – 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 3 9
Bud Site – 0 0 0 0 0 3 1 4 4 4 11 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 1 1 – 0 0 0 0 0
Cytoplasm – 161 63 209 270 254 394 446 201 248 178 257 322 540 587 31 76 27 – 164 69 75 422 494
Endoplasmic Reticulum – 2 0 0 0 0 0 0 0 0 2 0 4 0 1 3 1 0 – 1 0 1 2 1
Endosome – 1 1 3 31 6 5 14 5 4 4 7 4 5 4 1 7 2 – 0 2 0 6 6
Golgi – 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1 – 0 0 0 0 3
Mitochondria – 1 7 60 48 195 341 432 318 398 388 488 2 0 2 0 0 1 – 1 1 1 3 4
Nucleus – 7 0 3 9 18 36 48 38 63 63 81 18 3 1 2 2 0 – 5 2 4 15 17
Nuclear Periphery – 0 1 0 0 0 1 1 1 0 2 0 0 0 2 0 0 0 – 0 0 0 0 0
Nucleolus – 0 0 0 0 1 1 4 0 3 3 2 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – 1 0 0 0 0 2 3 0 1 1 3 0 0 0 0 0 0 – 0 0 0 9 8
Vac/Vac Membrane – 12 4 34 135 77 140 159 91 99 76 113 52 60 61 28 62 20 – 7 9 6 28 25
Unique Cell Count 173 68 273 416 434 736 889 506 667 570 720 368 582 633 52 126 45 184 90 91 508 594
Labelled Cell Count 187 80 315 494 561 942 1120 671 829 743 983 403 609 658 65 150 52 184 90 91 508 594


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.5 4.7 4.3 4.8 4.0 3.9 3.9 3.6 3.9 3.6 3.6 5.9 5.9 5.8 9.6 9.7 9.5 5.4 5.9 6.1
Std Deviation (1e-4) – 0.7 0.7 0.9 1.4 1.3 1.0 1.1 0.9 1.1 0.9 0.8 0.9 1.0 1.0 1.9 1.4 2.0 0.7 1.2 1.2
Intensity Change (Log2) – – – -0.13 0.03 -0.24 -0.27 -0.27 -0.37 -0.27 -0.4 -0.39 0.32 0.33 0.3 1.04 1.05 1.02 0.21 0.34 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.0 -4.6 -5.4 -6.2 -6.8 -8.2 -8.8 -9.9 -9.1 -1.2 0 0 -4.4 -4.8 -4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2.7 2.9 2.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 4.5 2.5 2.7 2.5 2.5 2.0 1.8 2.2 1.9 1.2 1.0 5.9 6.1 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9468 1.9506 1.3937 0.9023 1.0636 1.4366 0.1536 0.9047 0.2712 0.0796 -0.0471 0.3081 1.8764 2.2365 2.1085 1.8846 2.2099 2.3129
Actin 0.0175 0.0008 0.0121 0.0012 0.0719 0.0061 0.0495 0.0037 0.0037 0.0132 0.0003 0.0007 0.0816 0.0004 0.0006 0.0098 0.0191 0.0007
Bud 0.0012 0.007 0.0018 0.0003 0.0035 0.0003 0.0011 0.0008 0.0002 0.0004 0.0001 0.0001 0.0007 0.0001 0.0002 0.0003 0.0007 0.0001
Bud Neck 0.0024 0.0004 0.0036 0.0013 0.0039 0.0047 0.0023 0.0003 0.0005 0.0019 0.0007 0.0017 0.0018 0.0002 0.0007 0.0008 0.0015 0.0018
Bud Periphery 0.0012 0.0012 0.0011 0.0001 0.0059 0.0004 0.0011 0.0003 0.0002 0.0001 0.0001 0.0001 0.001 0 0.0001 0.0003 0.001 0
Bud Site 0.0029 0.0061 0.0032 0.0005 0.0083 0.0006 0.0069 0.0011 0.0009 0.0011 0.0002 0.0002 0.0056 0.0008 0.0007 0.0005 0.0021 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0 0.0004 0 0.0002 0 0.0001 0 0 0 0.0005 0.0001 0 0 0.0002 0
Cytoplasm 0.4893 0.6121 0.5451 0.4455 0.4516 0.5628 0.4891 0.7198 0.5784 0.6356 0.5712 0.5674 0.2169 0.7121 0.627 0.5928 0.4254 0.6634
Cytoplasmic Foci 0.024 0.0068 0.0188 0.0116 0.01 0.0116 0.0239 0.0056 0.0142 0.0225 0.0123 0.0065 0.0551 0.0019 0.0058 0.0046 0.0292 0.0036
Eisosomes 0.0003 0 0.0002 0 0.0011 0 0.0003 0 0 0 0 0 0.0009 0 0 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.0048 0.0044 0.0045 0.0061 0.0086 0.0045 0.0075 0.0062 0.0062 0.0051 0.004 0.0035 0.0222 0.0029 0.0045 0.0027 0.0016 0.0044
Endosome 0.0218 0.0039 0.0096 0.0037 0.0442 0.0125 0.0387 0.0045 0.0201 0.0067 0.0365 0.0058 0.047 0.0008 0.008 0.0029 0.034 0.0026
Golgi 0.0037 0.0002 0.0044 0.0008 0.0117 0.0014 0.0166 0.0003 0.0145 0.0018 0.0014 0.0026 0.0179 0.0001 0.0085 0.0017 0.0673 0.0001
Lipid Particles 0.0058 0.0002 0.0079 0.0069 0.0077 0.0013 0.0118 0.0002 0.0029 0.0153 0.0032 0.0023 0.0552 0.0001 0.0015 0.01 0.065 0.0001
Mitochondria 0.003 0.0009 0.0022 0.0008 0.0354 0.001 0.0086 0.0012 0.0229 0.0009 0.0006 0.0156 0.0084 0.0002 0.0023 0.0243 0.0384 0.0004
None 0.1329 0.1618 0.1523 0.209 0.0833 0.1158 0.1352 0.1025 0.1358 0.1112 0.1139 0.0473 0.1791 0.1307 0.0699 0.1463 0.1583 0.0543
Nuclear Periphery 0.012 0.0077 0.0141 0.0162 0.0427 0.0156 0.0181 0.0077 0.0113 0.0075 0.0123 0.01 0.0315 0.0055 0.0108 0.0118 0.002 0.0097
Nucleolus 0.0018 0.0005 0.0011 0.0009 0.0021 0.001 0.0006 0.0002 0.0008 0.0011 0.0007 0.0004 0.0031 0.0002 0.0005 0.0003 0.0004 0.0018
Nucleus 0.2447 0.1728 0.1822 0.2501 0.1757 0.2369 0.1543 0.1384 0.1733 0.12 0.227 0.3186 0.194 0.1398 0.2479 0.1592 0.1201 0.2389
Peroxisomes 0.0051 0.0002 0.01 0.0256 0.0077 0.0008 0.0119 0.0004 0.0029 0.0267 0.0004 0.0014 0.0559 0 0.0017 0.0191 0.0277 0.0001
Punctate Nuclear 0.02 0.0103 0.0226 0.0177 0.0145 0.0206 0.0149 0.0044 0.0072 0.0265 0.0113 0.0139 0.0104 0.0034 0.0072 0.0099 0.0021 0.0162
Vacuole 0.0043 0.0023 0.002 0.0011 0.0062 0.0017 0.0058 0.0019 0.0028 0.002 0.0028 0.0014 0.0082 0.0006 0.0019 0.0019 0.0021 0.001
Vacuole Periphery 0.001 0.0003 0.001 0.0005 0.0035 0.0005 0.0019 0.0003 0.0012 0.0003 0.001 0.0004 0.0029 0.0001 0.0003 0.0006 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.517 6.5758 2.4398 12.887 14.3061 7.352 14.1288 12.3407 21.082 14.6656
Translational Efficiency 5.5809 4.1723 10.5268 2.5526 2.045 3.601 2.2849 2.912 1.3548 1.8306

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘