Standard name
Description Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.93 0.77 0.65 0.59 0.54 0.50 0.40 0.37 0.31 0.36 0.88 0.93 0.93 0.60 0.60 0.60 0.89 0.77 0.83 0.83 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.10 0.22 0.12 0.45 0.46 0.49 0.63 0.60 0.68 0.68 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0.05 0.08 0.09 0.11 0.11 0.05 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.06 0.12 0.32 0.18 0.19 0.18 0.18 0.15 0.13 0.16 0.14 0.10 0.10 0.54 0.49 0.44 0 0.10 0.07 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 3
Bud 1 4 6 1 10 17 12 12 8 22 19 0 0 0 0 0 0 2 1 0 5 11
Bud Neck 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 9
Bud Site 0 0 0 0 0 3 1 4 4 4 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 1 1 0 0 0 0 0
Cytoplasm 161 63 209 270 254 394 446 201 248 178 257 322 540 587 31 76 27 164 69 75 422 494
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 0 2 0 4 0 1 3 1 0 1 0 1 2 1
Endosome 1 1 3 31 6 5 14 5 4 4 7 4 5 4 1 7 2 0 2 0 6 6
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 3
Mitochondria 1 7 60 48 195 341 432 318 398 388 488 2 0 2 0 0 1 1 1 1 3 4
Nucleus 7 0 3 9 18 36 48 38 63 63 81 18 3 1 2 2 0 5 2 4 15 17
Nuclear Periphery 0 1 0 0 0 1 1 1 0 2 0 0 0 2 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 4 0 3 3 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 2 3 0 1 1 3 0 0 0 0 0 0 0 0 0 9 8
Vac/Vac Membrane 12 4 34 135 77 140 159 91 99 76 113 52 60 61 28 62 20 7 9 6 28 25
Unique Cell Count 173 68 273 416 434 736 889 506 667 570 720 368 582 633 52 126 45 184 90 91 508 594
Labelled Cell Count 187 80 315 494 561 942 1120 671 829 743 983 403 609 658 65 150 52 184 90 91 508 594

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.7 4.3 4.8 4.0 3.9 3.9 3.6 3.9 3.6 3.6 5.9 5.9 5.8 9.6 9.7 9.5 5.4 5.9 6.1
Std Deviation (1e-4) 0.7 0.7 0.9 1.4 1.3 1.0 1.1 0.9 1.1 0.9 0.8 0.9 1.0 1.0 1.9 1.4 2.0 0.7 1.2 1.2
Intensity Change (Log2) -0.1 0.0 -0.2 -0.3 -0.3 -0.4 -0.3 -0.4 -0.4 0.3 0.3 0.3 1.0 1.0 1.0 0.2 0.3 0.4

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 3.0 0 0 1.7 2.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 1.9 1.0 0 4.8 4.7 4.3