Standard name
Description Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.09 0.06 0.08 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.4 0.3 0.47 0.52 0.54 0.48 0.42 0.4 0.16 0.1 0.11 0.22 0.45 0.67 0.69 0.56 0.54 0.67 0.69 0.71 0.77 0.79 0.82
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Mitochondria 0.44 0.49 0.28 0.33 0.22 0.39 0.39 0.28 0.7 0.71 0.76 0.58 0.42 0.14 0.17 0.1 0.13 0.06 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.08 0 0 0 0.05 0
Nucleolus 0.27 0.18 0.09 0.1 0.06 0.07 0.06 0 0.06 0.07 0.06 0 0.24 0.21 0.19 0.21 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0.06 0.13 0.11 0 0 0 0.06 0 0 0.06 0 0 0 0 0 0 0 0
SpindlePole 0.14 0.05 0.32 0.25 0.35 0.24 0.33 0.39 0.21 0.25 0.15 0.3 0.13 0.11 0.09 0.18 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.32 0.28 0.18 0.14 0.11 0.07 0.07 0 0.05 0.05 0.08 0.28 0.31 0.17 0.4 0.43 0.09 0.13 0.1 0.08 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 1 0 0 5 2 2 1 2 1 0 1 0 1 0 3 2 0 1 3
Bud 1 2 11 6 2 13 28 38 16 28 13 27 3 2 2 1 2 0 1 0 1 0 1
Bud Neck 0 0 0 0 2 0 2 3 1 2 1 1 0 0 0 0 1 0 0 0 0 0 0
Bud Site 1 0 1 0 1 1 6 2 3 4 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 4 9 2 6 12 5 22 23 8 10 9 5 5 11 14 15 12 2 2 3 1 0 0
Endoplasmic Reticulum 4 0 1 0 0 1 0 0 1 4 3 2 2 2 4 12 16 0 1 1 1 0 0
Endosome 79 70 120 112 162 175 199 176 42 34 22 59 91 253 266 232 176 136 180 207 137 261 305
Golgi 1 1 1 0 0 1 1 0 0 0 0 0 1 1 0 28 19 6 4 6 0 2 9
Mitochondria 88 114 72 70 65 143 186 121 182 244 151 157 85 52 64 40 41 11 13 11 3 5 4
Nucleus 2 2 5 2 0 4 5 2 3 0 3 0 1 1 0 2 0 0 2 1 0 0 0
Nuclear Periphery 12 8 3 0 0 1 2 1 2 3 1 1 6 17 10 9 24 17 5 11 6 16 11
Nucleolus 54 42 24 22 17 27 29 15 15 24 12 9 49 77 75 86 59 0 2 1 1 0 2
Peroxisomes 3 1 8 6 19 23 63 49 9 15 7 16 5 9 22 1 1 1 1 5 0 2 6
SpindlePole 27 11 81 53 104 87 157 170 54 86 30 82 26 42 35 73 52 7 5 6 7 11 3
Vac/Vac Membrane 43 74 72 38 43 39 31 30 10 16 10 23 56 118 64 164 140 19 33 29 13 23 22
Unique Cell Count 198 233 254 215 301 365 473 437 261 345 200 271 203 375 386 415 326 205 260 291 179 331 374
Labelled Cell Count 320 334 402 315 429 521 732 636 348 472 267 384 331 585 557 663 545 205 260 291 179 331 374


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.0 6.3 5.7 6.6 5.2 5.4 5.7 4.9 4.6 4.5 5.1 6.4 6.8 6.7 10.3 9.4 5.9 6.1 6.7
Std Deviation (1e-4) 1.0 1.3 0.9 0.7 1.3 0.6 1.2 1.0 1.0 0.9 0.8 1.0 1.1 0.9 1.1 2.1 1.7 1.1 0.9 1.2
Intensity Change (Log2) -0.15 0.07 -0.27 -0.22 -0.13 -0.37 -0.46 -0.47 -0.29 0.02 0.1 0.1 0.71 0.58 -0.09 -0.06 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.9 -2.8 -0.5 0.9 2.2 0.9 0 0 2.5 -1.8 -3.3 -3.3 0 -3.0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.4 0 2.8 3.0 0 1.8 0 0 0 1.9 2.3 2.3 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2
Endosome 1.0 1.5 0.2 -1.3 -1.8 -7.6 0 0 -6.2 -0.5 5.1 5.5 2.2 1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.9 3.6
Mitochondria 1.0 -1.8 2.8 2.9 -0.2 9.4 0 0 6.8 3.0 -4.5 -3.6 -6.3 -4.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2.4 1.2 0 3.5
Nucleolus 0.3 -1.7 -0.9 -1.6 -3.3 -1.6 0 0 -2.9 4.3 3.7 3.4 3.8 3.0
Peroxisomes -0.2 1.7 1.8 4.4 3.7 0.2 0 0 1.5 -0.4 -0.6 1.5 0 0
SpindlePole -1.7 0.7 -2.2 0.4 1.8 -2.9 0 0 -0.4 -4.8 -6.4 -7.3 -4.3 -4.5
Vacuole -2.7 -4.1 -5.6 -8.0 -7.7 -7.6 -8.1 -6.4 -5.9 -0.2 0.8 -3.6 2.9 3.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available