Standard name
Description Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.3 0.46 0.5 0.66 0.61 0.7 0.69 0.67 0.72 0.5 0.59 0.38 0.36 0.43 0.15 0.14 0.16 0.28 0.21 0.18 0.23 0.24 0.2
Endoplasmic Reticulum 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0.12 0 0.09 0.05 0.41 0.17 0.25 0.34 0.32 0.58 0.41 0.06 0 0 0.05 0.08 0.06 0.1 0 0.14 0.06 0.05 0.05
Nucleus 0.18 0.48 0.3 0.26 0.16 0.09 0.08 0.05 0 0 0.05 0.07 0.49 0.51 0.4 0.73 0.68 0.72 0.23 0.39 0.28 0.36 0.34 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.08
Vac/Vac Membrane 0.18 0.2 0.23 0.12 0.15 0 0.1 0 0 0 0.06 0 0.09 0.11 0.14 0.11 0.13 0.1 0.24 0.25 0.18 0.19 0.2 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud 0 0 0 1 0 2 2 3 1 2 2 1 0 0 0 1 0 0 0 3 2 8 12 18
Bud Neck 0 0 2 0 2 2 2 9 1 3 0 0 0 0 1 0 0 0 1 0 0 1 3 7
Bud Site 0 0 0 0 0 5 0 3 0 0 0 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 14 48 28 89 132 117 229 353 91 141 79 98 151 194 314 54 35 42 28 45 17 92 150 130
Endoplasmic Reticulum 3 0 1 0 0 0 0 0 0 0 0 0 2 0 1 21 12 11 0 0 1 1 3 2
Endosome 0 0 1 16 11 2 7 10 2 1 1 2 9 24 30 8 10 6 2 3 2 5 10 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 0 1 1 1 0 0 1
Mitochondria 15 20 0 17 9 79 54 128 46 63 91 68 23 13 18 19 21 16 10 8 13 22 32 35
Nucleus 7 78 18 46 32 18 25 25 6 5 8 12 195 273 293 254 169 187 23 82 27 146 213 220
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 0 4 1 0 2 2 0 0 0 0 1 0 0 1 0 12 14 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 4 5 1 11 8 1 8 5 4 4 2 2 1 2 1 3 9 5 27 42 49
Vac/Vac Membrane 7 33 14 22 30 2 34 22 6 6 10 6 36 61 102 40 33 26 24 52 17 76 125 142
Unique Cell Count 38 161 61 179 199 191 327 508 135 197 157 166 399 537 729 349 250 260 103 212 98 408 624 658
Labelled Cell Count 46 179 66 195 221 228 364 566 155 229 198 198 421 567 761 400 286 289 103 212 98 408 624 658


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.2 5.1 5.2 5.5 3.6 4.3 4.2 3.9 4.1 3.9 4.3 4.6 4.7 4.7 6.1 6.1 6.3 4.1 4.2 4.2
Std Deviation (1e-4) 0.5 0.6 0.9 1.7 1.9 1.4 1.2 1.4 1.5 1.7 1.6 2.2 1.0 1.1 0.9 1.4 1.5 1.4 0.8 1.1 1.1
Intensity Change (Log2) 0.03 0.12 -0.48 -0.23 -0.27 -0.37 -0.3 -0.36 -0.24 -0.12 -0.1 -0.09 0.28 0.27 0.31 -0.31 -0.27 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole -2.4 -1.9 -2.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available