Standard name | |
---|---|
Human Ortholog | |
Description | Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle; human lissencephaly-associated homolog CDK5 functionally complements null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 6 | 7 | 20 | 25 | 27 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 12 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 40 | 75 | 57 | 37 | 74 | 83 | 92 | 51 | 44 | 32 | 46 | 32 | 104 | 128 | 145 | 239 | 106 | 208 | 22 | 26 | 45 | 51 | 41 | 59 |
Endoplasmic Reticulum | 1 | 0 | 3 | 0 | 2 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 14 | 2 | 8 | 1 | 0 | 3 | 0 | 2 | 3 |
Endosome | 0 | 0 | 2 | 2 | 1 | 6 | 2 | 4 | 1 | 2 | 4 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | 7 | 13 | 9 |
Golgi | 0 | 0 | 0 | 2 | 10 | 4 | 7 | 4 | 3 | 4 | 2 | 3 | 1 | 1 | 7 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 2 | 0 | 0 | 0 | 0 | 7 | 17 | 10 | 23 | 13 | 39 | 10 | 0 | 2 | 1 | 2 | 2 | 3 | 0 | 0 | 1 | 0 | 4 | 2 |
Nucleus | 13 | 20 | 68 | 119 | 125 | 109 | 159 | 104 | 96 | 91 | 105 | 116 | 66 | 99 | 97 | 25 | 8 | 7 | 88 | 110 | 138 | 167 | 198 | 212 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 2 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 2 | 7 | 6 | 3 | 2 | 13 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 6 | 10 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 6 | 10 | 13 |
Vac/Vac Membrane | 77 | 75 | 118 | 97 | 108 | 134 | 147 | 120 | 90 | 54 | 108 | 97 | 141 | 183 | 194 | 234 | 39 | 76 | 8 | 13 | 31 | 30 | 43 | 65 |
Unique Cell Count | 112 | 124 | 206 | 232 | 278 | 301 | 396 | 271 | 228 | 184 | 282 | 243 | 264 | 365 | 399 | 404 | 133 | 262 | 139 | 165 | 250 | 299 | 365 | 431 |
Labelled Cell Count | 135 | 170 | 248 | 259 | 320 | 349 | 436 | 302 | 262 | 200 | 321 | 266 | 317 | 416 | 446 | 526 | 162 | 305 | 139 | 165 | 250 | 299 | 365 | 431 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 13.2 | 11.7 | 11.5 | 11.8 | 10.5 | 10.4 | 11.0 | 9.8 | 10.2 | 9.7 | 10.1 | 12.5 | 12.5 | 13.0 | 16.8 | 16.0 | 15.9 | 11.8 | 12.3 | 12.2 |
Std Deviation (1e-4) | 1.5 | 2.3 | 1.6 | 1.6 | 1.7 | 1.8 | 1.9 | 1.8 | 1.9 | 1.8 | 2.0 | 2.1 | 2.1 | 2.2 | 2.1 | 2.9 | 3.3 | 3.0 | 1.8 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.01 | -0.16 | -0.17 | -0.1 | -0.25 | -0.21 | -0.28 | -0.21 | 0.09 | 0.09 | 0.15 | 0.52 | 0.45 | 0.44 | 0.01 | 0.07 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.0 | -0.3 | 0 | -1.2 | -2.3 | -2.1 | -2.4 | -3.0 | -3.8 | 2.7 | 1.8 | 2.1 | 7.4 | 9.4 | 11.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | 0 | 4.7 | 3.9 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.9 | 2.7 | 0.7 | 1.7 | 1.2 | 2.0 | 3.3 | 1.0 | 3.2 | -1.9 | -1.5 | -2.3 | -8.7 | -5.8 | -8.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.2 | -4.0 | -2.8 | -4.7 | -2.8 | -3.7 | -5.5 | -4.2 | -3.7 | -0.8 | -1.6 | -2.0 | 0.2 | -5.0 | -6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.9223 | 6.1399 | 5.7814 | 5.4529 | 5.9654 | 5.9806 | 5.4616 | 6.2334 | 6.2761 | 4.9291 | 4.8542 | 5.843 | 6.2483 | 6.6608 | 6.2499 | 5.6786 | 5.5376 | 6.8559 |
Actin | 0.0004 | 0.0002 | 0 | 0 | 0.0006 | 0.0001 | 0.0301 | 0.0001 | 0.0037 | 0.0398 | 0.0081 | 0.0026 | 0.0013 | 0.0002 | 0.0138 | 0.0056 | 0.0083 | 0.0108 |
Bud | 0.0029 | 0.0031 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0012 | 0.0028 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0.0019 | 0.0001 | 0.0002 |
Bud Neck | 0.0039 | 0.0006 | 0.0004 | 0.0001 | 0.001 | 0.0015 | 0.0042 | 0.0005 | 0.0027 | 0.0009 | 0.0025 | 0.0039 | 0.0007 | 0.0003 | 0.0025 | 0.0027 | 0.002 | 0.0043 |
Bud Periphery | 0.0006 | 0.0004 | 0 | 0 | 0.0003 | 0 | 0.0021 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0007 | 0.001 | 0.0001 | 0.0002 |
Bud Site | 0.0029 | 0.0015 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.002 | 0.0015 | 0.0028 | 0.0035 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0037 | 0.0025 | 0.0004 | 0.0004 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0293 | 0.1058 | 0.0448 | 0.0051 | 0.0392 | 0.0603 | 0.0658 | 0.1261 | 0.0713 | 0.0372 | 0.0222 | 0.0404 | 0.0406 | 0.0929 | 0.0609 | 0.0859 | 0.0227 | 0.0804 |
Cytoplasmic Foci | 0.0009 | 0.0007 | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0077 | 0.0007 | 0.0024 | 0.0009 | 0.0056 | 0.0036 | 0.0053 | 0.0002 | 0.0038 | 0.0523 | 0.001 | 0.0004 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0007 | 0.0005 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0008 | 0.0009 | 0.0001 | 0.0021 | 0.0011 | 0.005 | 0.0012 | 0.0017 | 0.0005 | 0.0033 | 0.0015 | 0.0017 | 0.0013 | 0.0039 | 0.0033 | 0.0049 | 0.0017 |
Endosome | 0.0016 | 0.0011 | 0.0015 | 0 | 0.0025 | 0.0013 | 0.0221 | 0.0017 | 0.0054 | 0.0007 | 0.0214 | 0.0079 | 0.0094 | 0.0006 | 0.0068 | 0.0114 | 0.0074 | 0.0032 |
Golgi | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0045 | 0 | 0.0011 | 0.0012 | 0.006 | 0.0026 | 0.0008 | 0 | 0.0028 | 0.0067 | 0.0007 | 0.0012 |
Lipid Particles | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0057 | 0 | 0.0047 | 0.0009 | 0.0122 | 0.0015 | 0.0066 | 0 | 0.0037 | 0.0103 | 0.0006 | 0.0009 |
Mitochondria | 0.0005 | 0.0005 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.0106 | 0.0003 | 0.0012 | 0.0119 | 0.0084 | 0.008 | 0.0008 | 0.0003 | 0.0023 | 0.0043 | 0.0047 | 0.0012 |
None | 0.001 | 0.0019 | 0.0009 | 0 | 0.0004 | 0.0016 | 0.0118 | 0.0008 | 0.0012 | 0.0003 | 0.0172 | 0.002 | 0.001 | 0.0003 | 0.0004 | 0.0008 | 0.0299 | 0.0005 |
Nuclear Periphery | 0.0109 | 0.0157 | 0.0145 | 0.0035 | 0.0279 | 0.0122 | 0.0275 | 0.0145 | 0.0122 | 0.0075 | 0.0218 | 0.012 | 0.0199 | 0.013 | 0.016 | 0.0114 | 0.0468 | 0.0123 |
Nucleolus | 0.0028 | 0.0016 | 0.0011 | 0.0004 | 0.0021 | 0.0015 | 0.0044 | 0.0011 | 0.0014 | 0.0007 | 0.0052 | 0.0024 | 0.0012 | 0.0008 | 0.0008 | 0.0006 | 0.0047 | 0.0008 |
Nucleus | 0.9278 | 0.8545 | 0.9279 | 0.99 | 0.9136 | 0.914 | 0.7641 | 0.8416 | 0.8774 | 0.8827 | 0.8416 | 0.895 | 0.8934 | 0.8828 | 0.8694 | 0.7654 | 0.8464 | 0.8772 |
Peroxisomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0026 | 0 | 0.001 | 0.0063 | 0.0045 | 0.0006 | 0.0063 | 0 | 0.0024 | 0.0262 | 0.0002 | 0.0003 |
Punctate Nuclear | 0.0118 | 0.009 | 0.0059 | 0.0007 | 0.0046 | 0.0044 | 0.0194 | 0.0049 | 0.0065 | 0.0032 | 0.0163 | 0.0132 | 0.0082 | 0.0057 | 0.0027 | 0.0033 | 0.0162 | 0.0022 |
Vacuole | 0.0009 | 0.002 | 0.001 | 0 | 0.0024 | 0.0011 | 0.0068 | 0.0014 | 0.0016 | 0.0003 | 0.0012 | 0.0009 | 0.0014 | 0.0009 | 0.0023 | 0.0031 | 0.0012 | 0.0012 |
Vacuole Periphery | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0007 | 0.0002 | 0.0019 | 0.0002 | 0.0005 | 0.0001 | 0.0011 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.001 | 0.0012 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.4187 | 23.6386 | 27.906 | 27.9684 | 27.272 | 38.4416 | 36.6663 | 32.6298 | 36.9356 | 28.3727 |
Translational Efficiency | 2.3599 | 1.4403 | 1.1281 | 1.2662 | 1.3249 | 0.8982 | 1.1816 | 1.3441 | 1.1831 | 1.5253 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
248 | 226 | 2616 | 1745 | 1825 | 1233 | 1060 | 1267 | 2073 | 1459 | 3676 | 3012 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1108.98 | 1158.59 | 1233.85 | 1371.08 | 1031.55 | 1098.77 | 1272.56 | 1293.97 | 1040.81 | 1108.04 | 1245.01 | 1338.64 |
Standard Deviation | 160.83 | 236.66 | 173.85 | 194.76 | 155.94 | 178.98 | 187.57 | 217.87 | 158.54 | 190.30 | 178.78 | 208.31 |
Intensity Change Log 2 | — | 0.063137 | 0.153934 | 0.306079 | — | 0.091076 | 0.302920 | 0.326990 | — | 0.076668 | 0.227654 | 0.316195 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000717 | 0.001051 | 0.000357 | 0.001206 | 0.000262 | 0.001333 | 0.000819 | 0.002178 | 0.000317 | 0.001289 | 0.000490 | 0.001615 |
Bud Neck | 0.005238 | 0.012952 | 0.009742 | 0.010974 | 0.004627 | 0.011365 | 0.007162 | 0.011617 | 0.004700 | 0.011611 | 0.008999 | 0.011244 |
Bud Site | 0.004442 | 0.007432 | 0.005180 | 0.015049 | 0.002573 | 0.010451 | 0.006879 | 0.024672 | 0.002796 | 0.009984 | 0.005670 | 0.019097 |
Cell Periphery | 0.000236 | 0.000267 | 0.000119 | 0.000192 | 0.000112 | 0.000233 | 0.000208 | 0.000222 | 0.000127 | 0.000238 | 0.000145 | 0.000205 |
Cytoplasm | 0.112434* | 0.138643* | 0.240506* | 0.321719* | 0.132458* | 0.152154* | 0.304401* | 0.415389* | 0.130062* | 0.150061* | 0.258930* | 0.361122* |
Cytoplasmic Foci | 0.002768 | 0.002960 | 0.001033 | 0.006995 | 0.001442 | 0.004104 | 0.006156 | 0.013286 | 0.001601 | 0.003927 | 0.002510 | 0.009641 |
Eisosomes | 0.000033 | 0.000023 | 0.000010 | 0.000018 | 0.000009 | 0.000024 | 0.000026 | 0.000024 | 0.000012 | 0.000024 | 0.000015 | 0.000020 |
Endoplasmic Reticulum | 0.007859 | 0.006360 | 0.001498 | 0.002448 | 0.003612 | 0.006745 | 0.003719 | 0.002344 | 0.004120 | 0.006685 | 0.002138 | 0.002404 |
Endosome | 0.000843 | 0.001287 | 0.000375 | 0.005619 | 0.000375 | 0.001607 | 0.003063 | 0.017341 | 0.000431 | 0.001557 | 0.001150 | 0.010550 |
Golgi | 0.000525 | 0.001009 | 0.000061 | 0.009857 | 0.000192 | 0.001174 | 0.001800 | 0.009758 | 0.000232 | 0.001148 | 0.000562 | 0.009815 |
Lipid Particles | 0.000816 | 0.000508 | 0.000208 | 0.001092 | 0.000160 | 0.001793 | 0.000846 | 0.001726 | 0.000238 | 0.001594 | 0.000392 | 0.001359 |
Mitochondria | 0.001216 | 0.001163 | 0.001456 | 0.005455 | 0.000619 | 0.003832 | 0.001246 | 0.007377 | 0.000691 | 0.003419 | 0.001396 | 0.006264 |
Mitotic Spindle | 0.003693 | 0.011271 | 0.009234 | 0.096289 | 0.001862 | 0.011406 | 0.022744 | 0.088423 | 0.002081 | 0.011385 | 0.013130 | 0.092980 |
None | 0.002525 | 0.010727 | 0.008154 | 0.007733 | 0.004606 | 0.007735 | 0.011420 | 0.015838 | 0.004357 | 0.008198 | 0.009096 | 0.011142 |
Nuclear Periphery | 0.000904 | 0.001015 | 0.001382 | 0.002477 | 0.001047 | 0.001847 | 0.001568 | 0.001618 | 0.001030 | 0.001718 | 0.001435 | 0.002116 |
Nuclear Periphery Foci | 0.001676 | 0.001031 | 0.000246 | 0.002497 | 0.000672 | 0.002576 | 0.002483 | 0.004366 | 0.000792 | 0.002336 | 0.000891 | 0.003283 |
Nucleolus | 0.001068 | 0.001709 | 0.000423 | 0.000602 | 0.000362 | 0.001257 | 0.000570 | 0.000943 | 0.000446 | 0.001327 | 0.000465 | 0.000746 |
Nucleus | 0.838083* | 0.786460* | 0.708845* | 0.449972* | 0.840495* | 0.765821* | 0.577435* | 0.315810* | 0.840207* | 0.769018* | 0.670952* | 0.393536* |
Peroxisomes | 0.000153 | 0.000215 | 0.000097 | 0.000210 | 0.000064 | 0.000565 | 0.000187 | 0.000916 | 0.000075 | 0.000511 | 0.000123 | 0.000507 |
Vacuole | 0.014615 | 0.013730 | 0.010929 | 0.057900 | 0.004288 | 0.012993 | 0.046783 | 0.062336 | 0.005523 | 0.013107 | 0.021268 | 0.059766 |
Vacuole Periphery | 0.000158 | 0.000187 | 0.000145 | 0.001695 | 0.000163 | 0.000986 | 0.000485 | 0.003816 | 0.000162 | 0.000862 | 0.000243 | 0.002587 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.94 | 1.97 | -0.59 | 1.80 | -4.40 | -4.99 | -7.89 | -5.14 | 1.03 | -0.00 | -5.45 | -4.50 | -7.01 | 1.60 | -3.87 |
Bud Neck | -3.74 | -3.76 | -2.24 | 2.93 | 2.40 | -6.44 | -3.82 | -3.01 | 3.83 | 0.31 | -7.44 | -7.02 | -5.26 | 4.49 | 2.58 |
Bud Site | -1.54 | 0.13 | -1.10 | 1.04 | -2.73 | -4.81 | -5.19 | -6.32 | -0.13 | -2.42 | -5.12 | -4.13 | -7.27 | 0.86 | -3.89 |
Cell Periphery | -0.60 | 2.87 | 1.02 | 2.44 | -6.33 | -7.94 | -8.12 | -5.62 | 3.53 | 2.80 | -7.43 | -1.79 | -5.77 | 3.08 | -4.85 |
Cytoplasm | -0.78 | -8.29 | -12.04 | -10.04 | -7.35 | -1.27 | -11.28 | -21.88 | -19.66 | -9.67 | -1.37 | -15.38 | -25.73 | -22.18 | -12.63 |
Cytoplasmic Foci | 0.20 | 2.48 | -0.43 | -0.61 | -6.53 | -3.31 | -2.77 | -4.78 | -1.27 | -2.26 | -3.21 | -0.17 | -5.55 | -0.54 | -6.05 |
Eisosomes | 0.89 | 2.62 | 1.49 | 1.35 | -9.60 | -9.98 | -14.98 | -14.56 | -0.36 | 3.56 | -7.49 | -5.65 | -9.61 | 0.53 | -6.08 |
Endoplasmic Reticulum | 0.50 | 6.87 | 6.49 | 6.28 | -2.20 | -8.11 | -2.30 | 5.61 | 12.18 | 8.40 | -7.52 | 6.00 | 7.85 | 13.78 | 3.52 |
Endosome | -0.71 | 2.77 | -1.55 | -0.18 | -4.33 | -4.59 | -3.85 | -3.84 | -2.15 | -2.16 | -4.64 | -2.41 | -4.98 | -1.60 | -4.12 |
Golgi | -0.38 | 0.99 | -1.26 | -0.68 | -2.35 | -2.20 | -1.15 | -2.80 | -1.11 | -1.88 | -2.22 | 0.08 | -3.11 | -1.11 | -3.25 |
Lipid Particles | 0.49 | 1.70 | -3.52 | -8.83 | -18.35 | -2.93 | -15.09 | -12.97 | -0.33 | -3.12 | -2.92 | -9.48 | -20.48 | -0.87 | -15.80 |
Mitochondria | -0.31 | -1.28 | -1.95 | -3.00 | -1.57 | -3.52 | -7.18 | -3.04 | 1.35 | -1.28 | -3.56 | -8.21 | -5.27 | 1.07 | -1.85 |
Mitotic Spindle | -1.15 | -2.70 | -7.92 | -3.95 | -6.96 | -4.22 | -6.14 | -9.10 | -6.08 | -3.81 | -4.34 | -7.76 | -13.14 | -7.78 | -8.29 |
None | -3.00 | -15.17 | -14.66 | -0.40 | -1.70 | -2.50 | -6.19 | -8.60 | -4.73 | -3.14 | -3.38 | -9.70 | -11.57 | -4.22 | -3.64 |
Nuclear Periphery | -1.72 | -9.23 | -14.44 | -12.57 | -9.13 | -4.93 | -12.01 | -10.67 | -0.87 | 1.32 | -5.07 | -14.09 | -17.68 | -4.83 | -7.36 |
Nuclear Periphery Foci | 0.74 | 2.57 | 0.54 | -0.49 | -9.41 | -2.91 | -4.69 | -3.66 | 0.92 | 0.39 | -2.75 | 0.79 | -4.02 | 1.15 | -5.98 |
Nucleolus | -0.55 | 0.35 | 0.69 | 1.53 | 0.79 | -3.11 | -0.69 | -0.88 | 2.71 | -0.40 | -3.10 | -0.84 | -0.33 | 3.14 | 0.72 |
Nucleus | 2.14 | 7.03 | 21.30 | 16.54 | 27.69 | 8.30 | 23.06 | 46.90* | 33.89 | 18.33 | 8.55 | 23.23 | 54.77 | 36.99 | 35.08 |
Peroxisomes | -1.09 | 1.23 | 0.63 | 1.56 | -0.71 | -2.11 | -3.93 | -3.62 | 1.27 | -1.69 | -2.19 | -1.95 | -3.76 | 1.63 | -1.83 |
Vacuole | 0.08 | -0.04 | -21.37 | -23.02 | -26.50 | -10.52 | -18.96 | -26.65 | -23.60 | -10.46 | -9.71 | -20.37 | -39.66 | -34.45 | -28.36 |
Vacuole Periphery | -0.49 | 0.60 | -1.49 | -1.35 | -2.04 | -2.47 | -0.55 | -2.59 | 0.35 | -2.39 | -2.48 | 0.28 | -2.72 | 1.02 | -3.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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