Standard name
Human Ortholog
Description Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle; human lissencephaly-associated homolog CDK5 functionally complements null mutation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.36 0.6 0.28 0.16 0.27 0.28 0.23 0.19 0.19 0.17 0.16 0.13 0.39 0.35 0.36 0.59 0.8 0.79 0.16 0.16 0.18 0.17 0.11 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.07 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.16 0.33 0.51 0.45 0.36 0.4 0.38 0.42 0.49 0.37 0.48 0.25 0.27 0.24 0.06 0.06 0 0.63 0.67 0.55 0.56 0.54 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.69 0.6 0.57 0.42 0.39 0.45 0.37 0.44 0.39 0.29 0.38 0.4 0.53 0.5 0.49 0.58 0.29 0.29 0.06 0.08 0.13 0.1 0.12 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 0 2 0 1 0 1 0 1 0 1 0 0 0 0 1 0 0 1
Bud 1 0 0 0 0 0 3 0 0 0 3 1 0 0 0 0 0 1 5 6 7 20 25 27
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 4 12
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 7 4 2 0 0 1 0 0 0
Cytoplasm 40 75 57 37 74 83 92 51 44 32 46 32 104 128 145 239 106 208 22 26 45 51 41 59
Endoplasmic Reticulum 1 0 3 0 2 2 0 1 2 0 0 0 2 2 0 14 2 8 1 0 3 0 2 3
Endosome 0 0 2 2 1 6 2 4 1 2 4 2 2 0 0 1 0 0 1 3 4 7 13 9
Golgi 0 0 0 2 10 4 7 4 3 4 2 3 1 1 7 3 1 0 0 0 1 0 1 1
Mitochondria 2 0 0 0 0 7 17 10 23 13 39 10 0 2 1 2 2 3 0 0 1 0 4 2
Nucleus 13 20 68 119 125 109 159 104 96 91 105 116 66 99 97 25 8 7 88 110 138 167 198 212
Nuclear Periphery 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 3 2
Nucleolus 1 0 0 1 0 2 7 6 3 2 13 4 1 0 0 0 0 0 2 0 1 6 10 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 6 10 13
Vac/Vac Membrane 77 75 118 97 108 134 147 120 90 54 108 97 141 183 194 234 39 76 8 13 31 30 43 65
Unique Cell Count 112 124 206 232 278 301 396 271 228 184 282 243 264 365 399 404 133 262 139 165 250 299 365 431
Labelled Cell Count 135 170 248 259 320 349 436 302 262 200 321 266 317 416 446 526 162 305 139 165 250 299 365 431


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 13.2 11.7 11.5 11.8 10.5 10.4 11.0 9.8 10.2 9.7 10.1 12.5 12.5 13.0 16.8 16.0 15.9 11.8 12.3 12.2
Std Deviation (1e-4) 1.5 2.3 1.6 1.6 1.7 1.8 1.9 1.8 1.9 1.8 2.0 2.1 2.1 2.2 2.1 2.9 3.3 3.0 1.8 1.7 1.9
Intensity Change (Log2) -0.03 0.01 -0.16 -0.17 -0.1 -0.25 -0.21 -0.28 -0.21 0.09 0.09 0.15 0.52 0.45 0.44 0.01 0.07 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.0 -0.3 0 -1.2 -2.3 -2.1 -2.4 -3.0 -3.8 2.7 1.8 2.1 7.4 9.4 11.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 0 4.7 3.9 5.6 0 0 0 0 0 0 0
Nucleus 3.9 2.7 0.7 1.7 1.2 2.0 3.3 1.0 3.2 -1.9 -1.5 -2.3 -8.7 -5.8 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -4.0 -2.8 -4.7 -2.8 -3.7 -5.5 -4.2 -3.7 -0.8 -1.6 -2.0 0.2 -5.0 -6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9223 6.1399 5.7814 5.4529 5.9654 5.9806 5.4616 6.2334 6.2761 4.9291 4.8542 5.843 6.2483 6.6608 6.2499 5.6786 5.5376 6.8559
Actin 0.0004 0.0002 0 0 0.0006 0.0001 0.0301 0.0001 0.0037 0.0398 0.0081 0.0026 0.0013 0.0002 0.0138 0.0056 0.0083 0.0108
Bud 0.0029 0.0031 0.0001 0 0.0003 0.0001 0.0012 0.0028 0.0005 0.0003 0.0002 0.0006 0.0002 0.0001 0.0005 0.0019 0.0001 0.0002
Bud Neck 0.0039 0.0006 0.0004 0.0001 0.001 0.0015 0.0042 0.0005 0.0027 0.0009 0.0025 0.0039 0.0007 0.0003 0.0025 0.0027 0.002 0.0043
Bud Periphery 0.0006 0.0004 0 0 0.0003 0 0.0021 0.0003 0.0004 0.0004 0.0003 0.0003 0.0001 0 0.0007 0.001 0.0001 0.0002
Bud Site 0.0029 0.0015 0.0001 0 0.0009 0.0001 0.002 0.0015 0.0028 0.0035 0.0004 0.0003 0.0002 0.0004 0.0037 0.0025 0.0004 0.0004
Cell Periphery 0.0001 0.0001 0 0 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0293 0.1058 0.0448 0.0051 0.0392 0.0603 0.0658 0.1261 0.0713 0.0372 0.0222 0.0404 0.0406 0.0929 0.0609 0.0859 0.0227 0.0804
Cytoplasmic Foci 0.0009 0.0007 0.0004 0 0.0003 0.0003 0.0077 0.0007 0.0024 0.0009 0.0056 0.0036 0.0053 0.0002 0.0038 0.0523 0.001 0.0004
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0 0.0007 0.0005 0.0001 0 0 0.0002 0 0.0004 0.0001
Endoplasmic Reticulum 0.0007 0.0008 0.0009 0.0001 0.0021 0.0011 0.005 0.0012 0.0017 0.0005 0.0033 0.0015 0.0017 0.0013 0.0039 0.0033 0.0049 0.0017
Endosome 0.0016 0.0011 0.0015 0 0.0025 0.0013 0.0221 0.0017 0.0054 0.0007 0.0214 0.0079 0.0094 0.0006 0.0068 0.0114 0.0074 0.0032
Golgi 0.0001 0 0 0 0.0001 0 0.0045 0 0.0011 0.0012 0.006 0.0026 0.0008 0 0.0028 0.0067 0.0007 0.0012
Lipid Particles 0.0003 0 0 0 0.0001 0 0.0057 0 0.0047 0.0009 0.0122 0.0015 0.0066 0 0.0037 0.0103 0.0006 0.0009
Mitochondria 0.0005 0.0005 0.0002 0 0.0007 0.0002 0.0106 0.0003 0.0012 0.0119 0.0084 0.008 0.0008 0.0003 0.0023 0.0043 0.0047 0.0012
None 0.001 0.0019 0.0009 0 0.0004 0.0016 0.0118 0.0008 0.0012 0.0003 0.0172 0.002 0.001 0.0003 0.0004 0.0008 0.0299 0.0005
Nuclear Periphery 0.0109 0.0157 0.0145 0.0035 0.0279 0.0122 0.0275 0.0145 0.0122 0.0075 0.0218 0.012 0.0199 0.013 0.016 0.0114 0.0468 0.0123
Nucleolus 0.0028 0.0016 0.0011 0.0004 0.0021 0.0015 0.0044 0.0011 0.0014 0.0007 0.0052 0.0024 0.0012 0.0008 0.0008 0.0006 0.0047 0.0008
Nucleus 0.9278 0.8545 0.9279 0.99 0.9136 0.914 0.7641 0.8416 0.8774 0.8827 0.8416 0.895 0.8934 0.8828 0.8694 0.7654 0.8464 0.8772
Peroxisomes 0.0002 0 0 0 0 0 0.0026 0 0.001 0.0063 0.0045 0.0006 0.0063 0 0.0024 0.0262 0.0002 0.0003
Punctate Nuclear 0.0118 0.009 0.0059 0.0007 0.0046 0.0044 0.0194 0.0049 0.0065 0.0032 0.0163 0.0132 0.0082 0.0057 0.0027 0.0033 0.0162 0.0022
Vacuole 0.0009 0.002 0.001 0 0.0024 0.0011 0.0068 0.0014 0.0016 0.0003 0.0012 0.0009 0.0014 0.0009 0.0023 0.0031 0.0012 0.0012
Vacuole Periphery 0.0002 0.0004 0.0003 0 0.0007 0.0002 0.0019 0.0002 0.0005 0.0001 0.0011 0.0006 0.0006 0.0002 0.0004 0.001 0.0012 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4187 23.6386 27.906 27.9684 27.272 38.4416 36.6663 32.6298 36.9356 28.3727
Translational Efficiency 2.3599 1.4403 1.1281 1.2662 1.3249 0.8982 1.1816 1.3441 1.1831 1.5253

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
248 226 2616 1745 1825 1233 1060 1267 2073 1459 3676 3012

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1108.98 1158.59 1233.85 1371.08 1031.55 1098.77 1272.56 1293.97 1040.81 1108.04 1245.01 1338.64
Standard Deviation 160.83 236.66 173.85 194.76 155.94 178.98 187.57 217.87 158.54 190.30 178.78 208.31
Intensity Change Log 2 0.063137 0.153934 0.306079 0.091076 0.302920 0.326990 0.076668 0.227654 0.316195

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000717 0.001051 0.000357 0.001206 0.000262 0.001333 0.000819 0.002178 0.000317 0.001289 0.000490 0.001615
Bud Neck 0.005238 0.012952 0.009742 0.010974 0.004627 0.011365 0.007162 0.011617 0.004700 0.011611 0.008999 0.011244
Bud Site 0.004442 0.007432 0.005180 0.015049 0.002573 0.010451 0.006879 0.024672 0.002796 0.009984 0.005670 0.019097
Cell Periphery 0.000236 0.000267 0.000119 0.000192 0.000112 0.000233 0.000208 0.000222 0.000127 0.000238 0.000145 0.000205
Cytoplasm 0.112434* 0.138643* 0.240506* 0.321719* 0.132458* 0.152154* 0.304401* 0.415389* 0.130062* 0.150061* 0.258930* 0.361122*
Cytoplasmic Foci 0.002768 0.002960 0.001033 0.006995 0.001442 0.004104 0.006156 0.013286 0.001601 0.003927 0.002510 0.009641
Eisosomes 0.000033 0.000023 0.000010 0.000018 0.000009 0.000024 0.000026 0.000024 0.000012 0.000024 0.000015 0.000020
Endoplasmic Reticulum 0.007859 0.006360 0.001498 0.002448 0.003612 0.006745 0.003719 0.002344 0.004120 0.006685 0.002138 0.002404
Endosome 0.000843 0.001287 0.000375 0.005619 0.000375 0.001607 0.003063 0.017341 0.000431 0.001557 0.001150 0.010550
Golgi 0.000525 0.001009 0.000061 0.009857 0.000192 0.001174 0.001800 0.009758 0.000232 0.001148 0.000562 0.009815
Lipid Particles 0.000816 0.000508 0.000208 0.001092 0.000160 0.001793 0.000846 0.001726 0.000238 0.001594 0.000392 0.001359
Mitochondria 0.001216 0.001163 0.001456 0.005455 0.000619 0.003832 0.001246 0.007377 0.000691 0.003419 0.001396 0.006264
Mitotic Spindle 0.003693 0.011271 0.009234 0.096289 0.001862 0.011406 0.022744 0.088423 0.002081 0.011385 0.013130 0.092980
None 0.002525 0.010727 0.008154 0.007733 0.004606 0.007735 0.011420 0.015838 0.004357 0.008198 0.009096 0.011142
Nuclear Periphery 0.000904 0.001015 0.001382 0.002477 0.001047 0.001847 0.001568 0.001618 0.001030 0.001718 0.001435 0.002116
Nuclear Periphery Foci 0.001676 0.001031 0.000246 0.002497 0.000672 0.002576 0.002483 0.004366 0.000792 0.002336 0.000891 0.003283
Nucleolus 0.001068 0.001709 0.000423 0.000602 0.000362 0.001257 0.000570 0.000943 0.000446 0.001327 0.000465 0.000746
Nucleus 0.838083* 0.786460* 0.708845* 0.449972* 0.840495* 0.765821* 0.577435* 0.315810* 0.840207* 0.769018* 0.670952* 0.393536*
Peroxisomes 0.000153 0.000215 0.000097 0.000210 0.000064 0.000565 0.000187 0.000916 0.000075 0.000511 0.000123 0.000507
Vacuole 0.014615 0.013730 0.010929 0.057900 0.004288 0.012993 0.046783 0.062336 0.005523 0.013107 0.021268 0.059766
Vacuole Periphery 0.000158 0.000187 0.000145 0.001695 0.000163 0.000986 0.000485 0.003816 0.000162 0.000862 0.000243 0.002587

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.94 1.97 -0.59 1.80 -4.40 -4.99 -7.89 -5.14 1.03 -0.00 -5.45 -4.50 -7.01 1.60 -3.87
Bud Neck -3.74 -3.76 -2.24 2.93 2.40 -6.44 -3.82 -3.01 3.83 0.31 -7.44 -7.02 -5.26 4.49 2.58
Bud Site -1.54 0.13 -1.10 1.04 -2.73 -4.81 -5.19 -6.32 -0.13 -2.42 -5.12 -4.13 -7.27 0.86 -3.89
Cell Periphery -0.60 2.87 1.02 2.44 -6.33 -7.94 -8.12 -5.62 3.53 2.80 -7.43 -1.79 -5.77 3.08 -4.85
Cytoplasm -0.78 -8.29 -12.04 -10.04 -7.35 -1.27 -11.28 -21.88 -19.66 -9.67 -1.37 -15.38 -25.73 -22.18 -12.63
Cytoplasmic Foci 0.20 2.48 -0.43 -0.61 -6.53 -3.31 -2.77 -4.78 -1.27 -2.26 -3.21 -0.17 -5.55 -0.54 -6.05
Eisosomes 0.89 2.62 1.49 1.35 -9.60 -9.98 -14.98 -14.56 -0.36 3.56 -7.49 -5.65 -9.61 0.53 -6.08
Endoplasmic Reticulum 0.50 6.87 6.49 6.28 -2.20 -8.11 -2.30 5.61 12.18 8.40 -7.52 6.00 7.85 13.78 3.52
Endosome -0.71 2.77 -1.55 -0.18 -4.33 -4.59 -3.85 -3.84 -2.15 -2.16 -4.64 -2.41 -4.98 -1.60 -4.12
Golgi -0.38 0.99 -1.26 -0.68 -2.35 -2.20 -1.15 -2.80 -1.11 -1.88 -2.22 0.08 -3.11 -1.11 -3.25
Lipid Particles 0.49 1.70 -3.52 -8.83 -18.35 -2.93 -15.09 -12.97 -0.33 -3.12 -2.92 -9.48 -20.48 -0.87 -15.80
Mitochondria -0.31 -1.28 -1.95 -3.00 -1.57 -3.52 -7.18 -3.04 1.35 -1.28 -3.56 -8.21 -5.27 1.07 -1.85
Mitotic Spindle -1.15 -2.70 -7.92 -3.95 -6.96 -4.22 -6.14 -9.10 -6.08 -3.81 -4.34 -7.76 -13.14 -7.78 -8.29
None -3.00 -15.17 -14.66 -0.40 -1.70 -2.50 -6.19 -8.60 -4.73 -3.14 -3.38 -9.70 -11.57 -4.22 -3.64
Nuclear Periphery -1.72 -9.23 -14.44 -12.57 -9.13 -4.93 -12.01 -10.67 -0.87 1.32 -5.07 -14.09 -17.68 -4.83 -7.36
Nuclear Periphery Foci 0.74 2.57 0.54 -0.49 -9.41 -2.91 -4.69 -3.66 0.92 0.39 -2.75 0.79 -4.02 1.15 -5.98
Nucleolus -0.55 0.35 0.69 1.53 0.79 -3.11 -0.69 -0.88 2.71 -0.40 -3.10 -0.84 -0.33 3.14 0.72
Nucleus 2.14 7.03 21.30 16.54 27.69 8.30 23.06 46.90* 33.89 18.33 8.55 23.23 54.77 36.99 35.08
Peroxisomes -1.09 1.23 0.63 1.56 -0.71 -2.11 -3.93 -3.62 1.27 -1.69 -2.19 -1.95 -3.76 1.63 -1.83
Vacuole 0.08 -0.04 -21.37 -23.02 -26.50 -10.52 -18.96 -26.65 -23.60 -10.46 -9.71 -20.37 -39.66 -34.45 -28.36
Vacuole Periphery -0.49 0.60 -1.49 -1.35 -2.04 -2.47 -0.55 -2.59 0.35 -2.39 -2.48 0.28 -2.72 1.02 -3.26