Standard name
Description Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle; human lissencephaly-associated homolog CDK5 functionally complements null mutation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.36 0.6 0.28 0.16 0.27 0.28 0.23 0.19 0.19 0.17 0.16 0.13 0.39 0.35 0.36 0.59 0.8 0.79 0.16 0.16 0.18 0.17 0.11 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.07 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.16 0.33 0.51 0.45 0.36 0.4 0.38 0.42 0.49 0.37 0.48 0.25 0.27 0.24 0.06 0.06 0 0.63 0.67 0.55 0.56 0.54 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.69 0.6 0.57 0.42 0.39 0.45 0.37 0.44 0.39 0.29 0.38 0.4 0.53 0.5 0.49 0.58 0.29 0.29 0.06 0.08 0.13 0.1 0.12 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 0 2 0 1 0 1 0 1 0 1 0 0 0 0 1 0 0 1
Bud 1 0 0 0 0 0 3 0 0 0 3 1 0 0 0 0 0 1 5 6 7 20 25 27
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 4 12
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 7 4 2 0 0 1 0 0 0
Cytoplasm 40 75 57 37 74 83 92 51 44 32 46 32 104 128 145 239 106 208 22 26 45 51 41 59
Endoplasmic Reticulum 1 0 3 0 2 2 0 1 2 0 0 0 2 2 0 14 2 8 1 0 3 0 2 3
Endosome 0 0 2 2 1 6 2 4 1 2 4 2 2 0 0 1 0 0 1 3 4 7 13 9
Golgi 0 0 0 2 10 4 7 4 3 4 2 3 1 1 7 3 1 0 0 0 1 0 1 1
Mitochondria 2 0 0 0 0 7 17 10 23 13 39 10 0 2 1 2 2 3 0 0 1 0 4 2
Nucleus 13 20 68 119 125 109 159 104 96 91 105 116 66 99 97 25 8 7 88 110 138 167 198 212
Nuclear Periphery 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 3 2
Nucleolus 1 0 0 1 0 2 7 6 3 2 13 4 1 0 0 0 0 0 2 0 1 6 10 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 6 10 13
Vac/Vac Membrane 77 75 118 97 108 134 147 120 90 54 108 97 141 183 194 234 39 76 8 13 31 30 43 65
Unique Cell Count 112 124 206 232 278 301 396 271 228 184 282 243 264 365 399 404 133 262 139 165 250 299 365 431
Labelled Cell Count 135 170 248 259 320 349 436 302 262 200 321 266 317 416 446 526 162 305 139 165 250 299 365 431


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 13.2 11.7 11.5 11.8 10.5 10.4 11.0 9.8 10.2 9.7 10.1 12.5 12.5 13.0 16.8 16.0 15.9 11.8 12.3 12.2
Std Deviation (1e-4) 1.5 2.3 1.6 1.6 1.7 1.8 1.9 1.8 1.9 1.8 2.0 2.1 2.1 2.2 2.1 2.9 3.3 3.0 1.8 1.7 1.9
Intensity Change (Log2) -0.03 0.01 -0.16 -0.17 -0.1 -0.25 -0.21 -0.28 -0.21 0.09 0.09 0.15 0.52 0.45 0.44 0.01 0.07 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.0 -0.3 0 -1.2 -2.3 -2.1 -2.4 -3.0 -3.8 2.7 1.8 2.1 7.4 9.4 11.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 0 4.7 3.9 5.6 0 0 0 0 0 0 0
Nucleus 3.9 2.7 0.7 1.7 1.2 2.0 3.3 1.0 3.2 -1.9 -1.5 -2.3 -8.7 -5.8 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -4.0 -2.8 -4.7 -2.8 -3.7 -5.5 -4.2 -3.7 -0.8 -1.6 -2.0 0.2 -5.0 -6.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho85-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available