Standard name
Description Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.25 0.46 0.45 0.44 0.15 0.32 0.19 0.33 0.12 0.20 0.45 0.54 0.56 0.19 0.19 0.20 0.15 0.15 0.21 0.33 0.50 0.46
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome 0.11 0 0.07 0 0.05 0 0 0 0 0 0 0.16 0.12 0.09 0.07 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.10 0.06 0.07 0 0.57 0.25 0.63 0.54 0.68 0.63 0 0 0 0 0 0.05 0 0 0.06 0.09 0.08 0.06
Nucleus 0.26 0.29 0.36 0.35 0.26 0.41 0.39 0.34 0.31 0.41 0.39 0.14 0.10 0.11 0.53 0.52 0.51 0.32 0.35 0.31 0.09 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0.06 0.07 0.05
Vac/Vac Membrane 0.41 0.44 0.15 0.15 0.20 0 0.09 0 0 0.05 0 0.35 0.34 0.38 0.22 0.27 0.26 0.26 0.29 0.25 0.24 0.14 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 2 3 4
Bud 0 0 0 1 0 1 0 6 2 0 3 0 0 0 0 1 0 12 5 3 5 12 19
Bud Neck 0 1 2 0 4 0 3 2 2 2 0 0 0 0 0 0 0 3 3 1 4 5 8
Bud Site 0 0 0 0 0 2 2 1 1 2 3 0 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 85 82 66 40 65 7 52 28 57 12 20 119 248 230 38 43 34 64 62 47 71 200 177
Endoplasmic Reticulum 0 5 0 0 0 0 0 0 0 0 0 0 1 0 10 5 12 1 0 1 1 6 3
Endosome 35 11 10 4 8 0 3 0 0 0 0 42 55 38 14 12 4 17 16 6 8 17 16
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 2 5 5 1 6 2 0 4 2 4
Mitochondria 46 33 8 6 6 26 41 93 93 66 64 4 3 0 9 5 8 13 16 12 20 31 22
Nucleus 84 96 52 31 39 19 63 51 53 40 40 38 48 47 106 115 87 141 148 68 19 17 17
Nuclear Periphery 0 0 0 0 1 0 0 0 1 0 1 0 0 0 1 2 0 4 1 0 2 0 2
Nucleolus 2 0 0 0 0 0 0 2 1 1 0 0 2 0 3 1 1 10 4 2 1 1 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 3 2 0 3 9 0 8 4 4 3 0 1 2 0 5 2 3 32 25 10 13 29 19
Vac/Vac Membrane 136 145 22 13 30 1 15 3 5 5 3 92 159 157 44 61 45 112 120 55 51 58 74
Unique Cell Count 329 328 145 89 149 46 162 148 171 97 102 263 463 412 200 222 170 439 419 222 218 401 391
Labelled Cell Count 391 375 160 98 163 56 187 190 219 131 134 296 519 475 236 252 196 439 419 222 218 401 391

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.5 5.2 4.8 5.3 3.5 4.3 3.7 4.1 3.5 4.0 5.5 5.5 5.6 7.1 7.6 7.0 4.8 4.7 4.9
Std Deviation (1e-4) 1.1 0.7 1.2 0.8 1.7 0.7 1.1 1.2 1.7 0.7 1.5 1.5 1.6 1.9 1.7 2.0 1.9 1.8 1.6 1.9
Intensity Change (Log2) -0.1 0.0 -0.6 -0.3 -0.5 -0.3 -0.6 -0.4 0.1 0.1 0.1 0.5 0.6 0.4 -0.1 -0.1 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0