Standard name
Human Ortholog
Description Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.25 0.46 0.45 0.44 0.15 0.32 0.19 0.33 0.12 0.2 0.45 0.54 0.56 0.19 0.19 0.2 0.15 0.15 0.21 0.33 0.5 0.46
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome 0.11 0 0.07 0 0.05 0 0 0 0 0 0 0.16 0.12 0.09 0.07 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.1 0.06 0.07 0 0.57 0.25 0.63 0.54 0.68 0.63 0 0 0 0 0 0.05 0 0 0.06 0.09 0.08 0.06
Nucleus 0.26 0.29 0.36 0.35 0.26 0.41 0.39 0.34 0.31 0.41 0.39 0.14 0.1 0.11 0.53 0.52 0.51 0.32 0.35 0.31 0.09 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0.06 0.07 0.05
Vac/Vac Membrane 0.41 0.44 0.15 0.15 0.2 0 0.09 0 0 0.05 0 0.35 0.34 0.38 0.22 0.27 0.26 0.26 0.29 0.25 0.24 0.14 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 2 3 4
Bud 0 0 0 1 0 1 0 6 2 0 3 0 0 0 0 1 0 12 5 3 5 12 19
Bud Neck 0 1 2 0 4 0 3 2 2 2 0 0 0 0 0 0 0 3 3 1 4 5 8
Bud Site 0 0 0 0 0 2 2 1 1 2 3 0 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 85 82 66 40 65 7 52 28 57 12 20 119 248 230 38 43 34 64 62 47 71 200 177
Endoplasmic Reticulum 0 5 0 0 0 0 0 0 0 0 0 0 1 0 10 5 12 1 0 1 1 6 3
Endosome 35 11 10 4 8 0 3 0 0 0 0 42 55 38 14 12 4 17 16 6 8 17 16
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 2 5 5 1 6 2 0 4 2 4
Mitochondria 46 33 8 6 6 26 41 93 93 66 64 4 3 0 9 5 8 13 16 12 20 31 22
Nucleus 84 96 52 31 39 19 63 51 53 40 40 38 48 47 106 115 87 141 148 68 19 17 17
Nuclear Periphery 0 0 0 0 1 0 0 0 1 0 1 0 0 0 1 2 0 4 1 0 2 0 2
Nucleolus 2 0 0 0 0 0 0 2 1 1 0 0 2 0 3 1 1 10 4 2 1 1 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 3 2 0 3 9 0 8 4 4 3 0 1 2 0 5 2 3 32 25 10 13 29 19
Vac/Vac Membrane 136 145 22 13 30 1 15 3 5 5 3 92 159 157 44 61 45 112 120 55 51 58 74
Unique Cell Count 329 328 145 89 149 46 162 148 171 97 102 263 463 412 200 222 170 439 419 222 218 401 391
Labelled Cell Count 391 375 160 98 163 56 187 190 219 131 134 296 519 475 236 252 196 439 419 222 218 401 391


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.5 5.2 4.8 5.3 3.5 4.3 3.7 4.1 3.5 4.0 5.5 5.5 5.6 7.1 7.6 7.0 4.8 4.7 4.9
Std Deviation (1e-4) 1.1 0.7 1.2 0.8 1.7 0.7 1.1 1.2 1.7 0.7 1.5 1.5 1.6 1.9 1.7 2.0 1.9 1.8 1.6 1.9
Intensity Change (Log2) -0.11 0.04 -0.57 -0.27 -0.47 -0.33 -0.55 -0.37 0.08 0.09 0.12 0.46 0.55 0.44 -0.12 -0.13 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.6 2.8 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3395 -0.9479 -1.2177 -1.5875 -1.4289 -1.2398 0.7818 0.8977 0.6643 1.4862 0.2004 1.2091 2.6555 3.2192 3.3157 2.876 3.0606 3.4961
Actin 0.0158 0.0005 0.0031 0.0011 0.0051 0.0009 0.0159 0.0009 0.0015 0.0049 0.0006 0.0022 0.0003 0.0004 0.0003 0.0003 0.0004 0.0003
Bud 0.0003 0.0001 0.0004 0.0001 0.0003 0.0001 0.0004 0.0042 0.0053 0.0009 0.0006 0.0007 0.0001 0.0001 0.0001 0.0001 0 0.0006
Bud Neck 0.0014 0.0019 0.0013 0.0146 0.0073 0.0029 0.0022 0.0012 0.0007 0.0085 0.0013 0.0055 0.0018 0.0007 0.0009 0.0017 0.0009 0.0043
Bud Periphery 0.0004 0.0001 0.0022 0.0001 0.0006 0.0001 0.0005 0.0014 0.0015 0.001 0.0008 0.0023 0.0001 0.0001 0.0001 0.0002 0 0.0002
Bud Site 0.0009 0.0045 0.0011 0.0007 0.0016 0.0003 0.0049 0.0067 0.0036 0.0126 0.0006 0.0037 0.0021 0.0016 0.0006 0.0008 0.0001 0.0002
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0003 0.0002 0.0001 0.0004 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.1609 0.1771 0.113 0.0698 0.0765 0.1061 0.1207 0.2429 0.2132 0.1902 0.3902 0.1191 0.1634 0.135 0.0945 0.0766 0.1355 0.0966
Cytoplasmic Foci 0.0207 0.0091 0.0113 0.0087 0.0072 0.0126 0.0656 0.0137 0.0203 0.0412 0.0226 0.0066 0.0116 0.0035 0.0117 0.0034 0.0058 0.0042
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0003 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0056 0.0075 0.0053 0.0015 0.006 0.003 0.0059 0.0062 0.0071 0.0018 0.0039 0.0049 0.0039 0.0041 0.0033 0.0058 0.0028 0.0018
Endosome 0.0149 0.0093 0.0209 0.0057 0.0345 0.0091 0.0375 0.0073 0.0227 0.08 0.0073 0.0092 0.0068 0.0037 0.0088 0.005 0.0026 0.003
Golgi 0.0031 0.0003 0.0033 0.0036 0.0032 0.0009 0.0115 0.0003 0.003 0.0323 0.0003 0.0008 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001
Lipid Particles 0.0053 0.0017 0.0109 0.0109 0.0204 0.0042 0.0444 0.001 0.0022 0.0191 0.0005 0.0043 0.0009 0.0003 0.0025 0.0005 0.0006 0.0003
Mitochondria 0.0028 0.001 0.0046 0.0173 0.003 0.0016 0.0107 0.0011 0.0027 0.0096 0.0009 0.0027 0.0008 0.0005 0.0081 0.0012 0.0005 0.0005
None 0.1893 0.2249 0.1605 0.1927 0.1253 0.1328 0.2804 0.2943 0.2734 0.2697 0.1697 0.1981 0.246 0.2716 0.2369 0.2826 0.3208 0.2201
Nuclear Periphery 0.0436 0.0542 0.0562 0.0191 0.0948 0.0231 0.02 0.0272 0.0351 0.0072 0.0119 0.0263 0.0174 0.0156 0.0213 0.0363 0.0113 0.0091
Nucleolus 0.0064 0.0075 0.0097 0.0095 0.0302 0.0124 0.0045 0.0055 0.0051 0.0031 0.0032 0.0108 0.0061 0.0058 0.0088 0.013 0.0041 0.0092
Nucleus 0.4255 0.4271 0.5117 0.4769 0.4915 0.6057 0.2916 0.3442 0.3216 0.2865 0.2911 0.5386 0.4692 0.5298 0.516 0.493 0.4677 0.6032
Peroxisomes 0.0032 0.0002 0.0064 0.0066 0.0039 0.0006 0.0312 0.0005 0.0076 0.014 0.003 0.001 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001
Punctate Nuclear 0.0948 0.0674 0.0701 0.1592 0.0794 0.0807 0.0417 0.0344 0.0673 0.0116 0.0865 0.057 0.0653 0.0243 0.0823 0.0749 0.0452 0.0443
Vacuole 0.0036 0.0041 0.0053 0.0013 0.0056 0.002 0.0085 0.0059 0.0048 0.0041 0.0044 0.0046 0.0029 0.0024 0.0025 0.0031 0.0011 0.0014
Vacuole Periphery 0.0014 0.0011 0.0023 0.0006 0.0031 0.0006 0.0014 0.0008 0.0011 0.0013 0.0004 0.0012 0.0005 0.0003 0.0005 0.0011 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6786 16.3037 19.1553 20.7547 9.8527 28.861 16.4847 19.4706 24.9248 17.8573
Translational Efficiency 0.4012 0.4782 0.4497 0.5707 0.7501 0.3746 0.3013 0.4119 0.4516 0.4536

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase
Localization
Cell Percentages nucleus (34%), cytoplasm (5%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rmi1

Rmi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rmi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available