Standard name
Human Ortholog
Description Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.94 0.94 0.84 0.73 0.62 0.64 0.57 0.47 0.49 0.99 0.99 1.0 0.95 0.89 0.89 0.87 0.92 0.72 0.89 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.05 0.25 0.06 0.36 0.5 0.54 0.56 0.6 0.75 0.68 0 0 0 0 0 0 0.05 0 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0.06 0 0 0 0 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 2 2 1 0 1 2
Bud 0 0 1 0 1 1 0 0 3 3 1 4 0 0 0 0 0 0 0 4 1 0 1 2
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 0 2 0 0 0 1 0 0 1 3 0 2 0 0 0 5 8 4 0 1 0 0 0 0
Cytoplasm 222 319 123 102 118 152 120 102 109 142 64 85 202 201 188 444 332 227 199 301 99 84 187 156
Endoplasmic Reticulum 1 1 0 0 0 0 1 0 2 0 1 1 1 1 0 16 22 14 0 0 1 0 0 1
Endosome 0 0 1 0 1 0 1 1 1 0 0 0 0 0 0 11 11 8 2 0 1 0 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 5 2 1 1 1 0 0 0
Mitochondria 28 5 7 27 7 65 82 89 96 149 102 119 1 3 0 7 2 5 11 4 19 2 7 8
Nucleus 0 2 3 1 1 1 2 4 2 5 1 5 1 0 0 3 3 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 0 3 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 3 4 5 3 1 0 0 1 2
Vac/Vac Membrane 1 1 0 0 3 3 6 11 7 10 3 11 0 1 0 15 35 27 2 3 5 2 2 3
Unique Cell Count 230 324 128 109 125 180 165 164 170 247 136 175 204 204 188 467 373 256 229 329 138 95 212 187
Labelled Cell Count 252 332 137 130 132 223 212 210 221 316 175 230 205 207 188 505 424 295 229 329 138 95 212 187


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.5 4.3 3.9 4.3 4.3 3.7 4.1 4.0 3.8 3.8 3.9 4.9 5.0 5.2 6.3 7.7 7.9 3.9 4.0 4.9
Std Deviation (1e-4) 0.5 1.9 1.5 1.1 1.4 2.1 1.9 1.7 1.6 2.0 1.0 1.5 1.1 1.3 1.3 1.5 1.6 2.8 1.2 1.3 1.5
Intensity Change (Log2) -0.12 0.01 0.02 -0.19 -0.07 -0.1 -0.16 -0.16 -0.14 0.19 0.22 0.29 0.55 0.85 0.88 -0.14 -0.08 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.6 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.73 1.02 1.2197 0.6024 0.8922 0.9385 3.0732 2.9987 2.7973 3.3873 3.1171 2.9155 5.7065 3.8373 4.2352 4.1195 4.0862 4.1662
Actin 0.1198 0.0008 0.0067 0.0009 0.0147 0.0031 0.0695 0.0082 0.0137 0.0355 0.0155 0.0192 0.1446 0.0128 0.025 0.0161 0.0056 0.0037
Bud 0.004 0.0122 0.0022 0.0003 0.0018 0.0002 0.0007 0.0019 0.0008 0.0007 0.0032 0.0021 0.003 0.0045 0.0033 0.0056 0.0022 0.0006
Bud Neck 0.0141 0.0002 0.0004 0.0002 0.0011 0.0003 0.0076 0.002 0.001 0.0187 0.0013 0.0026 0.0025 0.0002 0.0011 0.0016 0.0004 0.0006
Bud Periphery 0.0059 0.0102 0.0051 0.0008 0.0024 0.0012 0.0017 0.0049 0.001 0.0011 0.0028 0.005 0.0172 0.0067 0.0075 0.0244 0.0042 0.0033
Bud Site 0.0286 0.004 0.0123 0.0001 0.041 0.0001 0.0149 0.0165 0.0071 0.0035 0.0092 0.0007 0.0064 0.0087 0.0083 0.0025 0.0011 0.0001
Cell Periphery 0.001 0.0003 0.0006 0.0001 0.0005 0.0001 0.0024 0.0006 0.0005 0.0005 0.0002 0.0002 0.002 0.0003 0.0005 0.0004 0.0002 0.0001
Cytoplasm 0.1378 0.353 0.3388 0.3786 0.4978 0.3198 0.2124 0.3779 0.2427 0.1429 0.3168 0.3219 0.2383 0.3257 0.298 0.2946 0.3622 0.3531
Cytoplasmic Foci 0.0218 0.0059 0.0095 0.0046 0.0191 0.0152 0.0133 0.0057 0.005 0.0266 0.0804 0.0071 0.0415 0.0108 0.0264 0.026 0.0215 0.0114
Eisosomes 0.0015 0.0001 0.0009 0.0001 0.0004 0.0002 0.0009 0.0002 0.0003 0.0002 0.0005 0.0001 0.0016 0.0001 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0095 0.0022 0.0039 0.002 0.0027 0.0019 0.0302 0.0059 0.0014 0.0014 0.0051 0.0012 0.02 0.0026 0.0035 0.004 0.0051 0.0024
Endosome 0.0302 0.0019 0.0067 0.0036 0.0246 0.0022 0.0328 0.0046 0.0026 0.0115 0.07 0.0041 0.0416 0.0045 0.0334 0.0111 0.0612 0.0033
Golgi 0.0112 0.0001 0.0043 0.0002 0.0037 0.0003 0.0098 0.001 0.0038 0.0066 0.0484 0.0024 0.0162 0.0008 0.0195 0.0076 0.0152 0.002
Lipid Particles 0.0158 0.0003 0.0059 0.0004 0.0059 0.0017 0.0128 0.0004 0.0026 0.0145 0.018 0.0035 0.0152 0.0011 0.0064 0.0194 0.0055 0.0015
Mitochondria 0.0188 0.0003 0.0016 0.0006 0.004 0.001 0.004 0.0208 0.0162 0.0365 0.0121 0.0007 0.0327 0.0006 0.0229 0.0097 0.0057 0.0013
None 0.5305 0.6025 0.5898 0.6013 0.3635 0.6457 0.5327 0.5375 0.6876 0.6844 0.3421 0.6184 0.3486 0.6087 0.5053 0.5226 0.4892 0.5999
Nuclear Periphery 0.0104 0.0008 0.0017 0.0013 0.0019 0.0014 0.0229 0.0054 0.0003 0.0012 0.003 0.0021 0.0158 0.0008 0.0077 0.0045 0.0055 0.0009
Nucleolus 0.0024 0.0002 0.0004 0.0001 0.0024 0.0002 0.0003 0.0002 0.0001 0.0007 0.0004 0.0003 0.0006 0.0006 0.0054 0.0003 0.0003 0.0002
Nucleus 0.0051 0.0021 0.0019 0.0022 0.0029 0.0012 0.004 0.0025 0.0006 0.0011 0.0013 0.0012 0.02 0.0028 0.0035 0.0025 0.002 0.0055
Peroxisomes 0.0154 0.0001 0.0049 0.0002 0.003 0.0006 0.0176 0.0003 0.0108 0.0078 0.0633 0.0037 0.0203 0.0035 0.0057 0.0243 0.0017 0.0019
Punctate Nuclear 0.0087 0.0016 0.0012 0.0006 0.0039 0.0028 0.0062 0.0009 0.0004 0.0025 0.0025 0.0023 0.0076 0.0022 0.0126 0.0205 0.0028 0.0075
Vacuole 0.0059 0.001 0.001 0.0014 0.0023 0.0006 0.0017 0.0019 0.0011 0.0016 0.0021 0.0008 0.0028 0.0018 0.0023 0.0014 0.005 0.0004
Vacuole Periphery 0.0016 0.0001 0.0003 0.0004 0.0007 0.0003 0.0018 0.0008 0.0003 0.0007 0.0016 0.0003 0.0016 0.0002 0.0011 0.0006 0.0031 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.839 1.9728 3.4157 2.5774 0.4769 1.4704 4.8204 7.4044 6.9102 4.4583
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
558 1313 818 468 1430 1739 359 27 1988 3052 1177 495

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 554.68 654.05 803.41 821.45 678.17 671.07 880.16 935.39 643.51 663.75 826.82 827.66
Standard Deviation 77.26 108.44 96.40 133.13 110.63 83.04 94.60 154.66 116.44 95.18 102.16 136.86
Intensity Change Log 2 0.237745 0.534481 0.566517 -0.015184 0.376119 0.463921 0.104126 0.449526 0.510986

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000019 0.000683 0.000157 0.027933 0.000042 0.000848 0.000121 0.000300 0.000036 0.000777 0.000146 0.026425
Bud Neck 0.004576 0.030875 0.005234 0.014589 0.001718 0.045464 0.002855 0.005625 0.002520 0.039187 0.004509 0.014100
Bud Site 0.000480 0.012141 0.008363 0.108981* 0.002711 0.022640 0.002018 0.013695 0.002085 0.018123 0.006427 0.103784*
Cell Periphery 0.000151 0.000143 0.000130 0.000313 0.000108 0.000207 0.000103 0.000231 0.000120 0.000180 0.000121 0.000308
Cytoplasm 0.493108* 0.450620* 0.361632* 0.305660* 0.650589* 0.386805* 0.357459* 0.238111* 0.606386* 0.414259* 0.360359* 0.301976*
Cytoplasmic Foci 0.139583 0.224604* 0.011085 0.042917 0.068159 0.276015* 0.017591 0.046621 0.088207 0.253897* 0.013070 0.043119
Eisosomes 0.000166 0.000120 0.000034 0.000094 0.000043 0.000228 0.000058 0.000074 0.000077 0.000181 0.000041 0.000093
Endoplasmic Reticulum 0.000205 0.000724 0.001747 0.002634 0.000427 0.000510 0.001915 0.001440 0.000365 0.000602 0.001798 0.002569
Endosome 0.002936 0.021789 0.001825 0.050041 0.001932 0.024510 0.002247 0.041478 0.002214 0.023339 0.001954 0.049574
Golgi 0.000500 0.005305 0.000069 0.035496 0.000192 0.010846 0.000093 0.018861 0.000278 0.008463 0.000076 0.034588
Lipid Particles 0.006866 0.007229 0.001171 0.004609 0.002124 0.007168 0.001620 0.003190 0.003455 0.007194 0.001308 0.004532
Mitochondria 0.002527 0.012235 0.000657 0.025942 0.000408 0.021458 0.000272 0.001219 0.001002 0.017490 0.000540 0.024594
Mitotic Spindle 0.000143 0.002934 0.005506 0.132040* 0.000612 0.010429 0.007590 0.002784 0.000480 0.007204 0.006142 0.124990*
None 0.010520 0.009584 0.005917 0.007200 0.014630 0.002927 0.010857 0.003914 0.013477 0.005791 0.007424 0.007020
Nuclear Periphery 0.000410 0.000491 0.001378 0.001393 0.000354 0.000288 0.001307 0.001235 0.000370 0.000375 0.001356 0.001385
Nuclear Periphery Foci 0.000150 0.000474 0.000510 0.004254 0.000775 0.000263 0.000824 0.001769 0.000600 0.000353 0.000606 0.004119
Nucleolus 0.002128 0.002630 0.000717 0.002774 0.000750 0.002865 0.000498 0.001119 0.001137 0.002764 0.000650 0.002684
Nucleus 0.312673* 0.133997* 0.558402* 0.104349 0.235311* 0.095357 0.497751* 0.288068* 0.257025* 0.111980 0.539903* 0.114370
Peroxisomes 0.002301 0.013951 0.000269 0.015552 0.000433 0.027077 0.000206 0.007333 0.000957 0.021430 0.000250 0.015104
Vacuole 0.020098 0.067457 0.034995 0.099270 0.018487 0.061507 0.094439 0.291374* 0.018939 0.064067 0.053126 0.109749
Vacuole Periphery 0.000460 0.002013 0.000202 0.013957 0.000194 0.002590 0.000177 0.031559 0.000269 0.002342 0.000194 0.014917

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.46 -9.44 -2.51 -2.26 -2.43 -4.05 -6.18 -3.36 2.77 -0.80 -4.58 -10.43 -2.50 -2.22 -2.43
Bud Neck -11.03 -0.35 -2.06 10.05 -1.38 -18.99 -0.99 -1.01 15.47 -0.21 -22.21 -2.12 -5.18 18.08 -2.07
Bud Site -10.64 -3.64 -7.00 -4.71 -5.37 -10.59 0.17 -1.49 3.66 -1.55 -13.01 -2.59 -6.69 -3.47 -5.79
Cell Periphery 0.81 1.31 0.59 -0.02 -0.65 -3.97 1.38 -0.57 1.77 -0.95 -3.79 0.28 -1.30 1.98 -1.31
Cytoplasm 3.47 12.54 12.36 10.30 1.58 27.95 24.15 8.58 3.14 1.35 24.91 30.64 23.57 9.19 1.56
Cytoplasmic Foci -10.18 22.25 20.27 32.72 -2.81 -33.01* 18.36 5.35 27.34 -2.03 -33.57 28.43 22.03 51.43 -2.79
Eisosomes 3.01 8.39 6.09 6.14 -5.69 -7.12 -5.22 -2.36 5.27 -0.36 -6.64 5.10 0.46 6.83 -4.56
Endoplasmic Reticulum -5.29 -14.15 -11.22 -9.07 -1.97 -0.97 -10.12 -3.18 -3.05 1.87 -3.79 -16.63 -10.91 -10.00 -1.48
Endosome -12.46 1.62 -3.90 2.99 -4.19 -15.94 -1.66 -1.88 0.83 -1.82 -20.42 -0.26 -4.45 3.83 -4.43
Golgi -6.34 2.33 -3.78 -1.40 -4.01 -11.58 3.93 -1.01 0.62 -1.03 -13.29 3.69 -4.03 0.15 -4.14
Lipid Particles -0.31 6.95 4.60 5.50 -1.06 -6.63 1.32 -0.05 6.12 -0.99 -6.60 6.27 1.85 6.27 -0.99
Mitochondria -4.67 1.34 -1.77 2.17 -3.14 -12.35 1.30 -0.59 12.17 -0.89 -13.35 1.06 -2.90 5.13 -3.19
Mitotic Spindle -4.15 -3.54 -8.34 -8.00 -7.19 -5.36 -1.66 -1.45 -1.21 -1.30 -6.00 -3.66 -8.38 -7.58 -7.39
None 0.45 2.07 0.75 0.45 -2.54 7.31 3.64 6.52 0.08 1.59 5.31 5.60 2.97 -1.85 -2.06
Nuclear Periphery -1.35 -14.92 -10.95 -10.43 -4.73 0.69 -10.59 -3.41 -3.73 2.28 -0.11 -16.03 -11.02 -11.21 -4.40
Nuclear Periphery Foci -1.97 -6.70 -4.95 -3.64 -3.60 3.39 -2.45 -2.09 -3.06 -1.28 1.17 -2.73 -4.16 -4.51 -3.48
Nucleolus -0.68 1.40 0.75 3.68 -1.96 -3.13 4.79 0.94 3.30 -0.94 -3.51 1.76 -0.43 3.19 -2.38
Nucleus 16.48 -18.02 6.11 -7.73 23.14 21.87 -14.14 0.53 -2.29 4.92 25.77 -28.01 2.92 -10.40 23.12
Peroxisomes -7.65 1.89 -0.99 6.19 -2.96 -15.66 5.40 -1.34 9.10 -1.48 -18.16 2.24 -2.46 11.27 -3.14
Vacuole -14.81 -8.94 -19.25 -15.21 -15.31 -15.98 -13.35 -5.81 -5.15 -3.23 -21.79 -15.30 -20.13 -16.50 -13.09
Vacuole Periphery -5.47 1.19 -3.02 -1.49 -3.37 -8.53 1.52 -1.01 -0.79 -1.01 -11.05 1.33 -3.31 -1.39 -3.39