Standard name
Description Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.11 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.96 0.96 0.94 0.88 0.76 0.76 0.75 0.77 0.6 0.72 0.99 0.99 0.99 0.91 0.94 0.95 0.95 0.95 0.91 0.97 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.14 0.28 0.26 0.33 0.26 0.5 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 7 4 1 2 0 0 0 0 0 0 1 0 0 0 0 1
Bud 1 1 2 0 0 0 4 7 11 11 12 8 0 0 0 0 0 0 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 5 5 4 9 19 9 6 8 11 12 12 0 8 5 24 33 27 0 0 0 0 0 0
Cytoplasm 386 393 230 204 322 300 336 270 254 337 210 207 183 198 239 201 304 232 380 385 227 127 244 205
Endoplasmic Reticulum 2 9 5 3 6 4 5 5 10 5 3 5 0 1 1 5 9 9 5 7 6 1 7 4
Endosome 0 0 0 1 0 0 2 4 2 1 0 1 0 0 0 0 2 1 3 0 1 0 3 6
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 3 2 3 0 2 1 0 1 1
Mitochondria 1 1 2 1 17 49 124 91 111 113 176 91 2 0 0 2 3 2 0 2 6 0 3 0
Nucleus 0 1 2 0 1 3 6 3 3 3 4 0 0 0 1 0 2 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 6 12 10 20 13 11 11 13 11 0 0 0 12 18 5 0 3 5 0 3 0
Unique Cell Count 389 404 239 213 344 339 441 355 337 439 350 288 184 200 241 221 322 243 399 406 251 131 270 226
Labelled Cell Count 391 412 247 219 367 385 506 404 417 498 431 338 185 207 246 248 374 279 399 406 251 131 270 226


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 10.9 8.4 8.5 7.8 6.6 6.2 6.6 5.9 6.3 5.4 6.0 9.2 9.7 9.4 14.6 15.2 15.4 9.1 9.4 10.0
Std Deviation (1e-4) 1.0 1.5 1.1 1.7 1.3 1.2 1.2 1.2 1.2 1.1 1.0 0.9 1.6 1.3 1.3 2.2 2.1 2.2 1.4 1.6 1.7
Intensity Change (Log2) 0.02 -0.1 -0.34 -0.44 -0.35 -0.51 -0.42 -0.62 -0.48 0.13 0.21 0.17 0.8 0.86 0.88 0.11 0.17 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.9 0 2.0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.4 2.1 0 0 0.2 0.3 1.0 1.3 0 1.2 0 3.9 3.8 4.0
Cytoplasm -0.2 -1.4 -3.3 -6.7 -6.6 -6.7 -6.5 -9.9 -7.4 2.2 1.8 2.1 -2.3 -1.0 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0.6 0 0 0 0 0 0 0 0.5 1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.7 5.7 8.7 8.2 9.6 8.3 12.8 9.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.3 3.0 0 0 3.0 0 0 0 0 3.7 3.7 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hst2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available