Standard name
Description Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.98 0.97 0.91 0.83 0.7 0.69 0.67 0.62 0.62 0.98 0.98 0.97 0.92 0.95 0.9 0.98 0.94 0.93 0.94 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.21 0.26 0.39 0.39 0.48 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 5 5 1 5 1 6 0 1 3 0 0 0 0 0 0 0 1 1
Bud 0 0 1 3 1 3 9 7 5 11 5 11 0 1 3 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 6 3 3 8 14 14 24 19 20 24 15 22 12 9 21 28 15 28 0 0 0 0 0 0
Cytoplasm 174 126 146 274 463 306 515 435 252 403 178 278 322 560 587 373 205 441 172 121 151 229 407 403
Endoplasmic Reticulum 2 2 2 2 10 11 14 30 20 32 15 25 2 9 15 20 8 20 1 1 4 7 16 31
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 1 0 0 0 0 0 1
Mitochondria 0 2 4 6 21 50 133 162 144 236 136 210 0 0 7 3 3 7 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 3 4 1 2 0 0 2 0 2 5 0 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 0 0 17 59 2 7 4 9 0 1 0 23 1 21 0 3 5 2 6 4
Unique Cell Count 178 130 148 280 479 337 623 620 366 603 285 446 329 571 607 407 216 490 176 130 163 243 441 451
Labelled Cell Count 183 134 156 294 509 386 720 723 446 723 355 562 339 582 639 453 234 524 176 130 163 243 441 451


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 12.2 8.4 8.5 8.2 7.0 6.5 6.3 5.5 5.3 5.1 4.9 14.1 13.2 11.5 16.0 16.2 17.7 13.6 12.6 14.5
Std Deviation (1e-4) 1.9 1.9 1.7 1.1 1.2 0.9 1.0 1.0 0.9 0.8 0.8 0.8 1.9 1.8 1.6 3.1 3.3 4.0 2.0 2.1 2.2
Intensity Change (Log2) – – – 0.02 -0.04 -0.27 -0.37 -0.43 -0.63 -0.66 -0.71 -0.77 0.74 0.65 0.45 0.93 0.95 1.07 0.69 0.59 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 1.1 0 1.7 1.1 1.6 1.5 0 0 0 2.2 2.1 1.8
Cytoplasm -0.6 -1.3 -3.2 -5.0 -7.3 -7.3 -7.8 -8.2 -8.5 -0.6 -0.5 -1.3 -3.0 -1.9 -3.4
Endoplasmic Reticulum 0 0 0 0 1.9 2.1 2.1 2.0 2.2 0 0 0 1.9 0 1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.9 3.9 5.3 6.2 8.3 8.5 9.5 9.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.9 0 0 0 0 0 0 0 3.0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rqc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available