Standard name | |
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Description | Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 1 | 5 | 1 | 6 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 1 | 3 | 1 | 3 | 9 | 7 | 5 | 11 | 5 | 11 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 3 | 8 | 14 | 14 | 24 | 19 | 20 | 24 | 15 | 22 | 12 | 9 | 21 | 28 | 15 | 28 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 174 | 126 | 146 | 274 | 463 | 306 | 515 | 435 | 252 | 403 | 178 | 278 | 322 | 560 | 587 | 373 | 205 | 441 | 172 | 121 | 151 | 229 | 407 | 403 |
Endoplasmic Reticulum | 2 | 2 | 2 | 2 | 10 | 11 | 14 | 30 | 20 | 32 | 15 | 25 | 2 | 9 | 15 | 20 | 8 | 20 | 1 | 1 | 4 | 7 | 16 | 31 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 2 | 4 | 6 | 21 | 50 | 133 | 162 | 144 | 236 | 136 | 210 | 0 | 0 | 7 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 1 | 2 | 0 | 0 | 2 | 0 | 2 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 1 | 0 | 0 | 17 | 59 | 2 | 7 | 4 | 9 | 0 | 1 | 0 | 23 | 1 | 21 | 0 | 3 | 5 | 2 | 6 | 4 |
Unique Cell Count | 178 | 130 | 148 | 280 | 479 | 337 | 623 | 620 | 366 | 603 | 285 | 446 | 329 | 571 | 607 | 407 | 216 | 490 | 176 | 130 | 163 | 243 | 441 | 451 |
Labelled Cell Count | 183 | 134 | 156 | 294 | 509 | 386 | 720 | 723 | 446 | 723 | 355 | 562 | 339 | 582 | 639 | 453 | 234 | 524 | 176 | 130 | 163 | 243 | 441 | 451 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 15.2 | 12.2 | 8.4 | 8.5 | 8.2 | 7.0 | 6.5 | 6.3 | 5.5 | 5.3 | 5.1 | 4.9 | 14.1 | 13.2 | 11.5 | 16.0 | 16.2 | 17.7 | 13.6 | 12.6 | 14.5 |
Std Deviation (1e-4) | 1.9 | 1.9 | 1.7 | 1.1 | 1.2 | 0.9 | 1.0 | 1.0 | 0.9 | 0.8 | 0.8 | 0.8 | 1.9 | 1.8 | 1.6 | 3.1 | 3.3 | 4.0 | 2.0 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.04 | -0.27 | -0.37 | -0.43 | -0.63 | -0.66 | -0.71 | -0.77 | 0.74 | 0.65 | 0.45 | 0.93 | 0.95 | 1.07 | 0.69 | 0.59 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.2 | 1.1 | 0 | 1.7 | 1.1 | 1.6 | 1.5 | 0 | 0 | 0 | 2.2 | 2.1 | 1.8 |
Cytoplasm | -0.6 | -1.3 | -3.2 | -5.0 | -7.3 | -7.3 | -7.8 | -8.2 | -8.5 | -0.6 | -0.5 | -1.3 | -3.0 | -1.9 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1.9 | 2.1 | 2.1 | 2.0 | 2.2 | 0 | 0 | 0 | 1.9 | 0 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0.9 | 3.9 | 5.3 | 6.2 | 8.3 | 8.5 | 9.5 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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