Standard name
Description Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.55 0.85 0.78 0.77 0.70 0.63 0.59 0.51 0.50 0.35 0.44 0.53 0.55 0.70 0.54 0.47 0.45 0.22 0.39 0.42 0.31 0.26 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.29 0 0.13 0 0.29 0.34 0.24 0.54 0.48 0.66 0.50 0.13 0 0 0 0 0 0 0.08 0.05 0.09 0.13 0.11
Nucleus 0.14 0.10 0 0.06 0.10 0.07 0.13 0.16 0.13 0.14 0.15 0.17 0.13 0.06 0 0.10 0.24 0.15 0.34 0.16 0.10 0.12 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.11 0.11 0.05 0.12 0.06 0 0.07 0.05 0.06 0 0.07 0.31 0.45 0.35 0.39 0.31 0.44 0.29 0.28 0.32 0.38 0.38 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 1 0 3 6 8
Bud 0 1 0 0 0 1 2 5 4 2 11 3 0 0 1 1 0 0 4 1 4 0 15 8
Bud Neck 0 2 6 7 11 4 3 23 7 3 2 7 0 0 0 1 0 0 0 1 1 1 4 6
Bud Site 0 0 0 0 0 1 2 0 5 3 8 10 0 0 0 0 0 0
Cell Periphery 0 0 1 1 1 0 0 1 1 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Cytoplasm 88 80 204 189 215 180 251 249 198 180 106 131 120 160 254 126 104 111 57 82 132 65 108 127
Endoplasmic Reticulum 4 6 0 0 1 0 0 1 0 0 0 0 0 0 0 16 11 13 1 0 1 1 3 8
Endosome 1 0 0 1 0 0 0 1 1 0 0 0 2 11 7 9 4 14 6 0 1 2 5 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 4 1
Mitochondria 39 42 9 31 10 74 134 102 210 172 200 150 29 13 12 6 5 5 11 16 17 19 52 39
Nucleus 29 14 5 14 27 19 53 69 50 51 46 50 29 18 10 24 52 36 87 33 30 24 28 13
Nuclear Periphery 2 0 0 0 0 0 0 0 1 0 2 0 0 0 1 0 1 0 1 0 1 0 0 0
Nucleolus 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 0 0 1 4 3 2 11 8 4 2 6 7 0 0 0 2 0 0 7 5 8 3 9 7
Vac/Vac Membrane 73 16 26 11 33 15 13 30 18 20 9 21 71 130 128 91 68 108 74 59 102 81 158 118
Unique Cell Count 203 145 240 243 278 258 397 419 390 362 304 299 228 291 363 234 219 246 261 213 316 214 413 363
Labelled Cell Count 237 162 252 259 301 296 469 491 499 434 391 380 251 332 413 277 246 291 261 213 316 214 413 363

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.3 4.7 4.2 4.9 3.7 3.9 4.0 3.5 3.4 3.0 3.6 4.1 4.1 4.3 6.0 6.0 5.8 3.9 4.2 4.4
Std Deviation (1e-4) 1.0 0.5 1.2 1.3 1.5 1.4 1.1 1.2 2.0 1.3 0.7 0.6 1.3 1.0 1.3 1.8 1.3 1.2 1.2 2.2 1.8
Intensity Change (Log2) -0.2 0.1 -0.3 -0.3 -0.2 -0.4 -0.5 -0.7 -0.4 -0.2 -0.2 -0.1 0.4 0.4 0.3 -0.3 -0.2 -0.1

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0