Standard name
Human Ortholog
Description Putative zinc finger protein; YPR013C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.84 0.99 0.89 0.81 0.74 0.75 0.7 0.54 0.59 0.42 0.45 0.76 0.83 0.89 0.93 0.93 0.91 0.49 0.54 0.61 0.44 0.48 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0 0 0.07 0.05 0.2 0.2 0.25 0.54 0.47 0.67 0.51 0 0 0 0 0 0 0.13 0.07 0.19 0.1 0.09 0.06
Nucleus 0 0 0 0 0.05 0.07 0.07 0.08 0.08 0.08 0.08 0.1 0.06 0 0 0 0 0 0 0.08 0 0.09 0.05 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0.09 0.11 0.08
Vac/Vac Membrane 0.08 0.11 0 0 0.09 0.08 0.06 0.05 0 0 0.06 0.07 0.14 0.13 0.11 0.05 0.07 0.05 0.18 0.15 0.08 0.16 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3 1 5 6
Bud 1 0 0 1 1 0 0 3 8 2 9 5 0 0 0 0 0 1 0 3 0 4 14 13
Bud Neck 0 2 0 2 4 4 3 7 4 7 2 8 0 2 3 3 0 1 1 1 2 2 7 12
Bud Site 0 0 0 0 1 0 0 0 1 1 3 1 0 0 0 0 0 0
Cell Periphery 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 7 3 4 0 0 0 0 0 1
Cytoplasm 151 132 107 144 191 171 171 213 181 161 124 127 171 285 361 277 228 205 129 111 87 104 192 223
Endoplasmic Reticulum 0 3 0 0 0 1 0 0 0 0 0 0 0 0 2 31 11 8 2 0 1 2 4 6
Endosome 0 1 0 0 7 1 1 0 0 0 0 2 12 31 8 3 2 3 4 3 1 3 4 7
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 1 0 0 2 0
Mitochondria 75 7 0 11 11 45 45 75 182 128 197 144 3 2 0 3 5 7 34 14 27 24 38 27
Nucleus 4 6 0 2 12 16 16 25 26 21 24 29 13 4 3 1 1 0 10 15 0 20 19 19
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 2 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 4 6 1 2 6 3 6 2 13 0 0 1 3 0 0 11 9 3 21 44 32
Vac/Vac Membrane 17 17 2 3 21 18 14 15 11 7 17 20 31 45 46 15 17 12 47 31 11 37 42 46
Unique Cell Count 215 157 108 161 237 230 227 306 336 275 295 285 224 344 406 298 244 225 261 205 144 236 402 422
Labelled Cell Count 248 169 110 169 254 257 252 344 417 333 380 349 230 369 424 343 267 246 261 205 144 236 402 422


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.1 4.7 4.3 4.7 4.0 4.0 4.2 3.3 3.4 3.2 3.5 5.1 5.1 5.2 5.8 5.8 5.5 4.8 5.4 5.5
Std Deviation (1e-4) 0.4 0.8 0.7 1.5 1.2 1.5 1.5 1.7 1.3 0.7 1.4 0.8 1.1 1.2 1.5 1.8 2.0 2.0 1.4 2.4 1.8
Intensity Change (Log2) -0.12 -0.01 -0.25 -0.25 -0.18 -0.53 -0.47 -0.56 -0.42 0.12 0.12 0.13 0.3 0.3 0.21 0.03 0.2 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0262 -0.7588 -0.9071 -1.0381 -1.2271 -0.7395 -0.1822 -0.4822 -0.5957 -0.3433 0.0618 -0.5833 -0.7327 -0.9726 -1.0155 -0.7197 -0.9443 -1.1855
Actin 0.0023 0.0005 0.0005 0.0001 0.0002 0.0003 0.0356 0.0005 0.0091 0.0134 0.0404 0.0013 0.0233 0.0002 0.0238 0.0015 0.0044 0.0109
Bud 0 0.0022 0.0002 0 0 0 0.002 0.0033 0.0036 0.0065 0.0005 0.0001 0.0032 0.0077 0.0005 0.0001 0.0005 0.0002
Bud Neck 0.0002 0.0028 0.0001 0.0001 0.0001 0.0013 0.0113 0.0005 0.0032 0.0011 0.0013 0.0018 0.0019 0 0.0005 0.0002 0.0004 0.0006
Bud Periphery 0.0001 0.0005 0.0006 0 0 0 0.0057 0.0046 0.002 0.0073 0.0005 0.0001 0.0023 0.0007 0.0007 0.0001 0.0005 0.0004
Bud Site 0.0003 0.0131 0.0003 0 0 0 0.0236 0.0175 0.0125 0.0185 0.002 0.0001 0.0057 0.0007 0.0009 0.0003 0.0005 0.0002
Cell Periphery 0 0.0002 0 0 0 0 0.0013 0.0004 0.0004 0.0004 0.0001 0 0.0008 0.0002 0.0001 0 0.0001 0.0001
Cytoplasm 0.0296 0.0394 0.0391 0.0414 0.0432 0.0784 0.051 0.0727 0.0714 0.0644 0.0729 0.1116 0.1129 0.1078 0.0674 0.0529 0.1986 0.1664
Cytoplasmic Foci 0.0127 0.0077 0.0039 0.0035 0.0035 0.0075 0.0406 0.0072 0.0051 0.0287 0.0336 0.0126 0.0269 0.0031 0.0109 0.0323 0.0233 0.0061
Eisosomes 0.0005 0.0002 0.0001 0 0.0001 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.001 0.0011 0.0005 0.0003 0.0005 0.0009 0.0043 0.0022 0.0025 0.0012 0.003 0.0012 0.0054 0.0011 0.0022 0.0012 0.0047 0.003
Endosome 0.0018 0.0014 0.0007 0.0003 0.0009 0.0026 0.018 0.0012 0.0091 0.046 0.0601 0.0026 0.0306 0.0011 0.0101 0.0162 0.0244 0.0051
Golgi 0.0003 0.0002 0.0001 0 0 0.0001 0.011 0.0001 0.0015 0.0079 0.046 0.0006 0.0045 0.0002 0.0052 0.0031 0.0121 0.0023
Lipid Particles 0.0013 0.0009 0.0002 0.0003 0.0004 0.0003 0.0116 0.0004 0.0016 0.0235 0.0252 0.0041 0.0106 0.0001 0.008 0.0192 0.0212 0.0013
Mitochondria 0.0054 0.0006 0.0005 0.0002 0.0004 0.0003 0.0111 0.0012 0.001 0.0059 0.0126 0.0006 0.0266 0.0002 0.0044 0.0007 0.0056 0.0058
None 0.8931 0.8321 0.9018 0.8231 0.8944 0.7402 0.6658 0.7926 0.7803 0.7282 0.6241 0.6747 0.6952 0.8592 0.8284 0.7066 0.5911 0.6833
Nuclear Periphery 0.0041 0.0032 0.002 0.0021 0.0087 0.0069 0.0098 0.0039 0.0039 0.0036 0.0053 0.0058 0.0153 0.0011 0.0052 0.0076 0.0089 0.0061
Nucleolus 0.0015 0.0031 0.0016 0.0009 0.0017 0.0033 0.0038 0.0035 0.0028 0.0016 0.001 0.0031 0.0033 0.0002 0.001 0.0011 0.0016 0.0012
Nucleus 0.0193 0.044 0.0345 0.117 0.0156 0.0696 0.0343 0.0588 0.0714 0.007 0.0299 0.1412 0.0148 0.0136 0.0141 0.0863 0.0475 0.0833
Peroxisomes 0.0039 0.0004 0.0004 0.0001 0.0001 0.0002 0.0217 0.0035 0.0006 0.02 0.0136 0.0047 0.0028 0 0.0052 0.0093 0.0329 0.0003
Punctate Nuclear 0.0221 0.0456 0.0123 0.0103 0.0295 0.0872 0.0281 0.0246 0.0157 0.0059 0.0255 0.0329 0.0068 0.0019 0.0092 0.0603 0.0179 0.0215
Vacuole 0.0004 0.0005 0.0004 0.0001 0.0003 0.0005 0.0066 0.0012 0.0017 0.0064 0.0013 0.0005 0.0045 0.0007 0.0014 0.0006 0.0026 0.0015
Vacuole Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0022 0.0002 0.0004 0.0022 0.0009 0.0002 0.0022 0.0001 0.0006 0.0004 0.0011 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2047 3.1018 4.6034 5.4034 11.9967 6.9358 10.7155 8.467 7.3662 8.9162
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
121 107 2555 1448 2159 172 184 1410 2280 279 2739 2858

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 725.17 721.37 779.96 778.78 647.28 677.17 819.33 752.97 651.41 694.12 782.60 766.05
Standard Deviation 72.74 99.02 87.60 101.36 73.33 104.99 83.42 110.96 75.35 104.97 87.88 106.99
Intensity Change Log 2 -0.007580 0.105081 0.102897 0.065128 0.340055 0.218202 0.027168 0.220678 0.158427

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000329 0.000131 0.001416 0.000019 0.000412 0.000210 0.000451 0.000027 0.000380 0.000137 0.000940
Bud Neck 0.012860 0.050738 0.008720 0.012747 0.005904 0.001589 0.001433 0.011303 0.006273 0.020438 0.008230 0.012035
Bud Site 0.003027 0.019925 0.003623 0.027641 0.000715 0.001843 0.001437 0.026757 0.000837 0.008778 0.003477 0.027205
Cell Periphery 0.000170 0.000225 0.000186 0.000384 0.000283 0.000399 0.000206 0.000455 0.000277 0.000332 0.000187 0.000419
Cytoplasm 0.445239* 0.274551* 0.250716* 0.240703* 0.394062* 0.345467* 0.271517* 0.337526* 0.396778* 0.318270* 0.252113* 0.288471*
Cytoplasmic Foci 0.253786* 0.117408 0.005080 0.008349 0.080600 0.079948 0.037384 0.022505 0.089791 0.094315 0.007250 0.015333
Eisosomes 0.000291 0.000121 0.000027 0.000051 0.000075 0.000099 0.000094 0.000086 0.000086 0.000107 0.000032 0.000068
Endoplasmic Reticulum 0.000307 0.001679 0.001051 0.006473 0.000635 0.000920 0.001889 0.001352 0.000618 0.001211 0.001107 0.003946
Endosome 0.007477 0.007942 0.001148 0.004543 0.002245 0.006510 0.003763 0.009178 0.002522 0.007059 0.001324 0.006830
Golgi 0.000427 0.002459 0.000057 0.001321 0.000303 0.004083 0.000338 0.003375 0.000310 0.003460 0.000076 0.002335
Lipid Particles 0.002634 0.011302 0.000631 0.001648 0.008054 0.004830 0.003071 0.001449 0.007766 0.007312 0.000795 0.001550
Mitochondria 0.000345 0.007213 0.001400 0.004637 0.002056 0.001119 0.005486 0.005897 0.001965 0.003456 0.001674 0.005259
Mitotic Spindle 0.000042 0.004796 0.007344 0.014201 0.000581 0.003362 0.000677 0.023976 0.000553 0.003912 0.006896 0.019024
None 0.001281 0.004754 0.009287 0.004377 0.007019 0.007484 0.005015 0.008485 0.006714 0.006437 0.009000 0.006403
Nuclear Periphery 0.000324 0.001138 0.001916 0.003310 0.001537 0.000740 0.001233 0.001312 0.001473 0.000892 0.001870 0.002324
Nuclear Periphery Foci 0.000313 0.001293 0.000157 0.001820 0.000208 0.000368 0.000715 0.000845 0.000213 0.000723 0.000195 0.001339
Nucleolus 0.000520 0.005697 0.001734 0.000897 0.001803 0.002848 0.001094 0.001659 0.001735 0.003940 0.001691 0.001273
Nucleus 0.187256* 0.385484* 0.696837* 0.608639* 0.476717* 0.511935* 0.615394* 0.491989* 0.461355* 0.463439* 0.691366* 0.551090*
Peroxisomes 0.007543 0.024388 0.000191 0.000463 0.002387 0.005251 0.000575 0.001262 0.002660 0.012591 0.000217 0.000857
Vacuole 0.075800 0.077719 0.009585 0.053286 0.014513 0.020219 0.047848 0.047028 0.017765 0.042271 0.012155 0.050198
Vacuole Periphery 0.000190 0.000840 0.000179 0.003094 0.000286 0.000576 0.000622 0.003111 0.000281 0.000677 0.000209 0.003102

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.91 0.10 -2.85 -2.12 -2.94 -1.20 -4.31 -4.84 0.85 -0.93 -1.40 -6.58 -4.54 0.36 -3.53
Bud Neck -2.31 0.54 0.30 2.50 -0.65 6.60 5.58 -3.80 -8.15 -7.51 -2.21 -3.01 -4.59 1.38 -1.04
Bud Site -3.22 -0.78 -6.14 0.29 -5.94 -1.66 -2.73 -8.30 -6.57 -7.23 -3.89 -4.95 -11.01 -3.63 -8.86
Cell Periphery -1.26 0.23 -2.36 -0.65 -2.80 -0.51 0.64 -0.17 0.48 -1.87 -0.32 1.01 -0.05 0.40 -3.95
Cytoplasm 4.89 8.88 9.93 2.57 3.50 2.26 7.39 8.88 1.40 -3.17 4.60 22.66 19.10 3.39 -3.74
Cytoplasmic Foci 5.56 13.00 12.86 6.77 -3.42 0.05 7.49 20.11 7.29 3.42 -0.35 27.60 25.31 10.04 -6.42
Eisosomes 2.57 4.38 3.81 4.19 -11.48 -2.73 -3.74 -0.07 2.06 2.98 -2.71 9.90 2.54 4.56 -6.71
Endoplasmic Reticulum -4.18 -13.21 -19.28 -11.08 -15.92 -0.74 -5.81 -8.33 -2.76 2.35 -2.43 -9.75 -19.11 -10.73 -14.41
Endosome -0.09 2.51 1.67 2.01 -4.96 -1.73 -2.24 -3.63 0.21 -1.22 -2.54 4.85 -3.46 1.35 -5.91
Golgi -1.62 3.94 -0.90 1.37 -2.39 -1.46 0.35 -3.27 0.78 -3.23 -1.85 5.94 -3.48 1.21 -4.40
Lipid Particles -2.89 6.70 2.71 3.19 -9.54 3.39 5.33 10.54 4.40 4.11 0.62 11.67 10.43 4.63 -7.12
Mitochondria -2.28 -4.23 -4.42 1.15 -2.56 1.76 -0.50 -1.77 -2.65 -0.11 -0.96 0.95 -2.31 -0.43 -3.02
Mitotic Spindle -1.68 -7.56 -6.59 -2.29 -0.66 -1.07 -0.78 -7.54 -5.01 -7.35 -1.73 -6.98 -9.65 -5.31 -3.05
None -2.87 -10.18 -5.97 0.57 7.08 -0.09 2.10 -0.07 0.05 -2.10 0.52 -1.70 1.76 0.65 4.01
Nuclear Periphery -4.61 -22.59 -17.54 -11.31 -8.46 4.37 -0.65 -4.41 -11.56 -4.02 3.03 -5.92 -10.24 -16.11 -6.94
Nuclear Periphery Foci -1.77 0.52 -7.65 -0.69 -7.78 -1.51 -4.46 -2.92 -2.11 1.57 -2.22 -2.45 -7.69 -2.13 -6.37
Nucleolus -1.62 -5.54 -2.30 1.50 4.42 -1.48 3.03 1.93 2.30 -1.17 -1.60 -0.60 3.53 2.26 3.35
Nucleus -5.02 -25.21 -19.57 -7.05 10.25 -1.08 -4.83 -0.54 0.83 4.45 0.25 -29.06 -9.84 -4.35 19.02
Peroxisomes -1.89 1.90 1.86 3.23 -2.25 -1.38 2.86 3.43 1.97 0.20 -3.02 5.20 4.44 3.76 -3.60
Vacuole -1.02 6.44 -2.28 -0.22 -19.71 -2.09 -6.13 -18.31 -10.07 -0.97 -4.54 -0.69 -24.98 -5.55 -24.28
Vacuole Periphery -2.32 0.37 -2.34 -1.18 -2.36 -0.79 -0.63 -2.95 -2.05 -2.33 -1.54 2.65 -3.59 -2.29 -3.83