Standard name
Description Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.05 0 0.06 0 0 0 0.07 0.11 0.1 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0 0.13 0 0 0.06 0 0.09 0.06 0.06 0 0 0 0 0 0
Nucleus 0.63 0.61 0.52 0.05 0 0 0.09 0.1 0.12 0.14 0.16 0.21 0.12 0 0 0.11 0.09 0.16 0.51 0.6 0.47 0.55 0.29 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.22 0.24 0.86 0.83 0.8 0.75 0.73 0.77 0.69 0.73 0.65 0.74 0.84 0.82 0.55 0.58 0.52 0.38 0.26 0.41 0.33 0.45 0.44
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.19 0.23 0.21 0.06 0.07 0.12 0.12 0.13 0.06 0.12 0.13 0.1 0.11 0.06 0 0.2 0.28 0.24 0.06 0.08 0.06 0.08 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 0 2 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 0 0 0 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 6 9 11 7 11 19 8 15 3 6 1 17 36 11 10 11 0 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 5 6 0 0 0 0 0 1
Endosome 0 1 2 3 7 11 15 5 3 6 2 5 1 2 5 2 3 0 0 0 1 1 9 9
Golgi 0 1 8 4 9 5 6 22 3 6 0 2 2 15 6 5 8 11 0 0 0 0 3 3
Mitochondria 0 0 0 8 6 9 13 3 26 10 16 9 2 16 12 10 9 10 0 0 0 0 0 5
Nucleus 44 69 86 12 7 12 29 40 25 36 20 45 8 4 3 12 14 28 64 96 117 121 84 53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 6 0 1 3
Nucleolus 15 25 40 191 200 217 250 285 162 173 91 136 49 211 276 63 91 90 47 42 104 72 131 146
Peroxisomes 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 10 12 7 9 1 2 0 4 0 1 12 0 0 0 2 0 3 1 16 21
Vac/Vac Membrane 13 26 35 14 17 34 41 52 13 29 16 21 7 14 13 23 43 42 7 13 14 17 38 67
Unique Cell Count 70 113 164 222 242 272 333 393 210 249 125 210 66 250 336 114 156 174 126 161 252 221 295 330
Labelled Cell Count 72 127 177 243 268 309 375 436 243 278 149 232 70 285 367 131 183 198 126 161 252 221 295 330


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.6 32.6 37.8 38.4 33.5 30.3 27.9 28.1 25.2 26.2 24.5 24.9 41.6 39.6 36.9 31.0 37.5 41.7 46.4 45.2 42.0
Std Deviation (1e-4) 11.0 10.1 10.8 11.8 10.5 8.5 8.2 7.9 7.6 7.8 8.4 7.7 12.0 12.6 14.6 12.7 14.6 16.5 15.8 13.8 16.5
Intensity Change (Log2) – – – 0.02 -0.17 -0.32 -0.44 -0.43 -0.58 -0.53 -0.62 -0.6 0.14 0.07 -0.03 -0.29 -0.01 0.14 0.3 0.26 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.4 0 -0.2 0.6 0.1 1.1 0 -0.4 0 1.4 2.7 2.1 1.1 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.7 -0.6 0 0 0.3 0 -1.4 0 0 0 0.5 0 -0.2 0.1 0.6
Mitochondria 0 0 0 0 0 4.7 0 4.7 0 0 3.3 0 0 0 0
Nucleus -10.5 -11.7 -11.6 -10.9 -10.9 -8.5 -8.3 -6.4 -6.2 -5.6 -12.3 -14.1 -7.2 -8.4 -7.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 12.2 11.7 11.4 10.8 10.5 10.2 9.0 8.2 7.8 7.0 12.2 12.6 5.2 6.2 5.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.4 -4.2 -2.5 -2.6 -2.4 -4.3 -2.7 -1.9 -3.1 -1.9 -4.8 -6.2 -0.2 1.3 0.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available