Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.05 0 0.06 0 0 0 0.07 0.11 0.1 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0 0.13 0 0 0.06 0 0.09 0.06 0.06 0 0 0 0 0 0
Nucleus 0.63 0.61 0.52 0.05 0 0 0.09 0.1 0.12 0.14 0.16 0.21 0.12 0 0 0.11 0.09 0.16 0.51 0.6 0.47 0.55 0.29 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.22 0.24 0.86 0.83 0.8 0.75 0.73 0.77 0.69 0.73 0.65 0.74 0.84 0.82 0.55 0.58 0.52 0.38 0.26 0.41 0.33 0.45 0.44
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.19 0.23 0.21 0.06 0.07 0.12 0.12 0.13 0.06 0.12 0.13 0.1 0.11 0.06 0 0.2 0.28 0.24 0.06 0.08 0.06 0.08 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 0 2 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 0 0 0 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 6 9 11 7 11 19 8 15 3 6 1 17 36 11 10 11 0 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 5 6 0 0 0 0 0 1
Endosome 0 1 2 3 7 11 15 5 3 6 2 5 1 2 5 2 3 0 0 0 1 1 9 9
Golgi 0 1 8 4 9 5 6 22 3 6 0 2 2 15 6 5 8 11 0 0 0 0 3 3
Mitochondria 0 0 0 8 6 9 13 3 26 10 16 9 2 16 12 10 9 10 0 0 0 0 0 5
Nucleus 44 69 86 12 7 12 29 40 25 36 20 45 8 4 3 12 14 28 64 96 117 121 84 53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 6 0 1 3
Nucleolus 15 25 40 191 200 217 250 285 162 173 91 136 49 211 276 63 91 90 47 42 104 72 131 146
Peroxisomes 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 10 12 7 9 1 2 0 4 0 1 12 0 0 0 2 0 3 1 16 21
Vac/Vac Membrane 13 26 35 14 17 34 41 52 13 29 16 21 7 14 13 23 43 42 7 13 14 17 38 67
Unique Cell Count 70 113 164 222 242 272 333 393 210 249 125 210 66 250 336 114 156 174 126 161 252 221 295 330
Labelled Cell Count 72 127 177 243 268 309 375 436 243 278 149 232 70 285 367 131 183 198 126 161 252 221 295 330


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.6 32.6 37.8 38.4 33.5 30.3 27.9 28.1 25.2 26.2 24.5 24.9 41.6 39.6 36.9 31.0 37.5 41.7 46.4 45.2 42.0
Std Deviation (1e-4) 11.0 10.1 10.8 11.8 10.5 8.5 8.2 7.9 7.6 7.8 8.4 7.7 12.0 12.6 14.6 12.7 14.6 16.5 15.8 13.8 16.5
Intensity Change (Log2) – – – 0.02 -0.17 -0.32 -0.44 -0.43 -0.58 -0.53 -0.62 -0.6 0.14 0.07 -0.03 -0.29 -0.01 0.14 0.3 0.26 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.4 0 -0.2 0.6 0.1 1.1 0 -0.4 0 1.4 2.7 2.1 1.1 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.7 -0.6 0 0 0.3 0 -1.4 0 0 0 0.5 0 -0.2 0.1 0.6
Mitochondria 0 0 0 0 0 4.7 0 4.7 0 0 3.3 0 0 0 0
Nucleus -10.5 -11.7 -11.6 -10.9 -10.9 -8.5 -8.3 -6.4 -6.2 -5.6 -12.3 -14.1 -7.2 -8.4 -7.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 12.2 11.7 11.4 10.8 10.5 10.2 9.0 8.2 7.8 7.0 12.2 12.6 5.2 6.2 5.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.4 -4.2 -2.5 -2.6 -2.4 -4.3 -2.7 -1.9 -3.1 -1.9 -4.8 -6.2 -0.2 1.3 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.7572 43.2088 38.7264 33.8092 38.4079 39.2132 32.9034 46.8859 38.2564 34.9174 34.3236 40.5921 37.0999 45.2592 39.0264 37.9545 36.1563 41.1705
Actin 0.0075 0 0.0047 0.0003 0.0004 0.0002 0.0055 0.0002 0.0103 0.0007 0.0014 0.0002 0.0105 0 0.0112 0.0012 0.0002 0.0001
Bud 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0005 0.0004 0.0001 0.0002 0 0.0003 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0003 0.0002 0.0002 0.0004 0.0005 0.0005 0.0002 0.0002 0.0003 0.0003 0.0004 0.0013 0.0001 0.0002 0.0002 0.0002 0.0004
Bud Periphery 0.0003 0.0001 0.0001 0.0001 0.0002 0.0003 0.0005 0.0001 0.0003 0.0008 0.0007 0.0004 0.0002 0.0001 0.0003 0.0003 0.0003 0.0002
Bud Site 0.0007 0.0001 0.0007 0.0003 0.0001 0.0001 0.0066 0.0001 0.0006 0.0004 0.0007 0.0001 0.0008 0 0.0025 0.0003 0.0001 0
Cell Periphery 0.0007 0.0007 0.0005 0.0005 0.0006 0.0004 0.0004 0.0002 0.0003 0.0003 0.0003 0.0005 0.0004 0.0002 0.0005 0.0003 0.0007 0.0002
Cytoplasm 0.002 0.0002 0.0008 0.0016 0.0002 0.0001 0.0111 0.0001 0.0023 0.001 0.0018 0.0001 0.0072 0 0.0059 0.0005 0.0038 0.0001
Cytoplasmic Foci 0.0101 0.0001 0.004 0.0492 0 0.0038 0.0103 0 0.0039 0.0002 0.0002 0 0.0156 0 0.0099 0.0032 0.0455 0
Eisosomes 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0012 0.0001 0.0004 0.0002 0.0001 0.0001 0.0023 0 0.0024 0.0001 0.0004 0.0002 0.0011 0 0.0005 0.0001 0.0002 0
Endosome 0.0103 0.0001 0.0029 0.0136 0.0001 0.0018 0.0152 0 0.0146 0.0003 0.002 0.0002 0.0042 0 0.0068 0.0023 0.001 0
Golgi 0.0017 0 0.0027 0.0028 0 0.0006 0.0041 0 0.0055 0 0.0002 0 0.0018 0 0.0027 0.0024 0.0002 0
Lipid Particles 0.0055 0.0003 0.0011 0.014 0.0001 0.0024 0.0114 0.0001 0.0022 0.0001 0.0005 0.0001 0.0104 0 0.0039 0.027 0.0076 0
Mitochondria 0.0043 0.0003 0.0013 0.0048 0.001 0.0011 0.0054 0.0012 0.0025 0.0038 0.0129 0.0014 0.0034 0.0008 0.0221 0.0096 0.003 0.0024
None 0.0025 0 0.0002 0.0039 0.0001 0 0.0117 0.0001 0.0012 0.0002 0.0007 0 0.0023 0 0.0016 0.0003 0.0039 0
Nuclear Periphery 0.027 0.0126 0.004 0.0598 0.0731 0.0429 0.0451 0.0425 0.0119 0.0311 0.0839 0.0499 0.0154 0.0045 0.0025 0.0213 0.0476 0.0045
Nucleolus 0.5849 0.5505 0.7167 0.5677 0.3416 0.5669 0.2764 0.4556 0.5122 0.2737 0.1509 0.4004 0.4184 0.4385 0.5723 0.5916 0.3522 0.4517
Nucleus 0.3205 0.4269 0.2555 0.2685 0.5734 0.3702 0.534 0.4961 0.4073 0.6705 0.7276 0.5355 0.4893 0.549 0.35 0.2986 0.5085 0.5371
Peroxisomes 0.0007 0 0.0002 0.0014 0 0.0006 0.0046 0 0.0003 0 0.0001 0 0.0026 0 0.0016 0.0304 0.0004 0
Punctate Nuclear 0.01 0.0021 0.0011 0.0034 0.001 0.001 0.0406 0.0006 0.019 0.0105 0.0034 0.0002 0.0118 0.0002 0.0012 0.0036 0.0072 0.0002
Vacuole 0.0038 0.0043 0.0017 0.003 0.002 0.0016 0.0064 0.0005 0.0011 0.0021 0.0031 0.0019 0.0013 0.0014 0.0021 0.002 0.0033 0.0007
Vacuole Periphery 0.0057 0.0011 0.0012 0.0044 0.0055 0.0053 0.0075 0.0023 0.0017 0.0031 0.0084 0.0085 0.0018 0.005 0.0019 0.0046 0.0139 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 219.0362 187.1174 145.7917 172.6736 149.2644 134.487 147.3012 143.655 133.5478 171.6951
Translational Efficiency 1.2235 1.2952 1.4962 1.3029 1.4138 1.6397 1.5001 1.1173 1.1671 1.1907

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Tif6

Tif6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available