Standard name | |
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Description | Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 6 | 9 | 11 | 7 | 11 | 19 | 8 | 15 | 3 | 6 | 1 | 17 | 36 | 11 | 10 | 11 | 0 | 2 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 2 | 3 | 7 | 11 | 15 | 5 | 3 | 6 | 2 | 5 | 1 | 2 | 5 | 2 | 3 | 0 | 0 | 0 | 1 | 1 | 9 | 9 |
Golgi | 0 | 1 | 8 | 4 | 9 | 5 | 6 | 22 | 3 | 6 | 0 | 2 | 2 | 15 | 6 | 5 | 8 | 11 | 0 | 0 | 0 | 0 | 3 | 3 |
Mitochondria | 0 | 0 | 0 | 8 | 6 | 9 | 13 | 3 | 26 | 10 | 16 | 9 | 2 | 16 | 12 | 10 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | 5 |
Nucleus | 44 | 69 | 86 | 12 | 7 | 12 | 29 | 40 | 25 | 36 | 20 | 45 | 8 | 4 | 3 | 12 | 14 | 28 | 64 | 96 | 117 | 121 | 84 | 53 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 0 | 1 | 3 |
Nucleolus | 15 | 25 | 40 | 191 | 200 | 217 | 250 | 285 | 162 | 173 | 91 | 136 | 49 | 211 | 276 | 63 | 91 | 90 | 47 | 42 | 104 | 72 | 131 | 146 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 10 | 12 | 7 | 9 | 1 | 2 | 0 | 4 | 0 | 1 | 12 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 16 | 21 |
Vac/Vac Membrane | 13 | 26 | 35 | 14 | 17 | 34 | 41 | 52 | 13 | 29 | 16 | 21 | 7 | 14 | 13 | 23 | 43 | 42 | 7 | 13 | 14 | 17 | 38 | 67 |
Unique Cell Count | 70 | 113 | 164 | 222 | 242 | 272 | 333 | 393 | 210 | 249 | 125 | 210 | 66 | 250 | 336 | 114 | 156 | 174 | 126 | 161 | 252 | 221 | 295 | 330 |
Labelled Cell Count | 72 | 127 | 177 | 243 | 268 | 309 | 375 | 436 | 243 | 278 | 149 | 232 | 70 | 285 | 367 | 131 | 183 | 198 | 126 | 161 | 252 | 221 | 295 | 330 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 41.6 | 32.6 | 37.8 | 38.4 | 33.5 | 30.3 | 27.9 | 28.1 | 25.2 | 26.2 | 24.5 | 24.9 | 41.6 | 39.6 | 36.9 | 31.0 | 37.5 | 41.7 | 46.4 | 45.2 | 42.0 |
Std Deviation (1e-4) | 11.0 | 10.1 | 10.8 | 11.8 | 10.5 | 8.5 | 8.2 | 7.9 | 7.6 | 7.8 | 8.4 | 7.7 | 12.0 | 12.6 | 14.6 | 12.7 | 14.6 | 16.5 | 15.8 | 13.8 | 16.5 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.17 | -0.32 | -0.44 | -0.43 | -0.58 | -0.53 | -0.62 | -0.6 | 0.14 | 0.07 | -0.03 | -0.29 | -0.01 | 0.14 | 0.3 | 0.26 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0.4 | 0 | -0.2 | 0.6 | 0.1 | 1.1 | 0 | -0.4 | 0 | 1.4 | 2.7 | 2.1 | 1.1 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.7 | -0.6 | 0 | 0 | 0.3 | 0 | -1.4 | 0 | 0 | 0 | 0.5 | 0 | -0.2 | 0.1 | 0.6 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.7 | 0 | 4.7 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 |
Nucleus | -10.5 | -11.7 | -11.6 | -10.9 | -10.9 | -8.5 | -8.3 | -6.4 | -6.2 | -5.6 | -12.3 | -14.1 | -7.2 | -8.4 | -7.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 12.2 | 11.7 | 11.4 | 10.8 | 10.5 | 10.2 | 9.0 | 8.2 | 7.8 | 7.0 | 12.2 | 12.6 | 5.2 | 6.2 | 5.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.4 | -4.2 | -2.5 | -2.6 | -2.4 | -4.3 | -2.7 | -1.9 | -3.1 | -1.9 | -4.8 | -6.2 | -0.2 | 1.3 | 0.6 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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