Standard name
Human Ortholog
Description H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.09 0.08 0.06 0.07 0.08 0.09 0.17 0.2
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.06 – 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.12 0.07 0.35 0.21 0.27 0 0.14 – 0 0.08 0.08 0.06 0 0 0.1 0.6 0.6 0.41 0.16 0.21 0.1 0.18 0.19 0.16
Mitochondria 0.97 0.86 0.68 0.92 0.69 0.85 0.7 – 0.88 0.82 0.87 0.87 0.98 0.94 0.85 0.06 0.08 0.15 0.73 0.6 0.73 0.63 0.53 0.52
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.09 0.18 0.07 0.18 0.16 0.16 – 0.15 0.15 0.12 0.13 0.2 0.13 0.1 0.14 0.22 0.29 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.16 0.09 0.06 0.13 0.08 0.13 – 0 0.06 0 0.07 0.14 0.17 0.21 0.15 0.13 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 6 5 4 2 2 – 1 3 3 3 0 4 1 10 8 7 3 10 13 16 42 52
Bud 0 0 0 1 0 1 0 – 6 3 3 0 1 0 0 0 0 1 0 1 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 2 4 2 0 2 2 – 2 0 1 2 1 0 1 5 1 1 0 0 1 0 1 0
Cytoplasm 0 3 3 2 5 16 18 – 18 13 14 5 0 3 1 3 2 1 0 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 1 0 – 0 1 6 3 1 0 1 4 2 6 0 2 0 0 1 0
Endosome 0 0 1 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4 10 44 31 56 9 44 – 7 28 20 11 5 10 25 71 54 35 9 32 16 32 47 41
Mitochondria 33 120 85 134 141 250 221 – 329 291 215 162 128 223 212 7 7 13 45 95 117 111 135 134
Nucleus 0 0 0 0 0 2 4 – 4 1 2 2 0 0 0 0 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 3 4 – 11 1 5 9 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 6 12 22 10 36 48 49 – 56 55 29 25 26 32 24 17 20 25 0 4 2 2 6 4
Peroxisomes 0 0 0 0 2 0 0 – 2 2 0 0 0 1 2 2 0 0 1 1 5 9 14 15
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 22 11 9 27 22 41 – 11 22 8 14 18 41 52 18 12 8 0 5 0 0 1 2
Unique Cell Count 34 140 125 146 205 293 314 376 356 246 187 131 238 249 118 90 85 62 159 160 179 253 256
Labelled Cell Count 45 172 176 194 272 357 385 448 422 306 236 180 314 319 137 107 102 62 159 160 179 253 256


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.3 77.3 64.9 61.0 57.3 34.5 40.7 – 30.0 30.4 29.2 26.3 100.1 92.9 103.1 56.3 50.4 53.1 78.3 83.0 82.8
Std Deviation (1e-4) 21.6 23.6 19.5 18.1 18.3 13.1 14.7 – 11.5 11.1 11.2 9.8 29.5 33.7 30.7 20.6 17.2 20.7 23.8 25.7 24.9
Intensity Change (Log2) – – – -0.09 -0.18 -0.91 -0.67 – -1.12 -1.09 -1.15 -1.3 0.62 0.52 0.67 -0.2 -0.37 -0.29 0.27 0.35 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 0 0 0 – 0 0 0 0 0 0 0 1.2 1.2 1.0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.4 1.5 – 1.2 0 1.4 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -2.6 -1.5 -9.0 -5.0 – -10.7 -7.4 -6.5 -6.7 -6.4 -7.9 -5.9 3.9 3.6 0.9
Mitochondria 5.0 0.1 4.1 0.5 – 5.0 3.2 4.5 4.0 6.4 6.5 3.9 -10.0 -8.8 -7.5
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.7 0 -0.3 -0.5 – -0.7 -0.6 -1.5 -1.0 0.5 -1.1 -2.2 -0.7 0.8 2.0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 1.2 -0.4 1.2 – -2.8 -1.0 -2.3 -0.4 1.2 2.2 2.9 1.6 1.1 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 87.4592 77.3009 82.484 70.5869 82.9284 78.4285 122.2763 104.4032 111.8712 113.5425 108.0777 109.5248 94.7929 95.7898 92.6787 66.8448 72.8101 95.0414
Actin 0.0014 0.0005 0.0004 0.0036 0 0.0001 0.0011 0.0007 0.001 0 0.0019 0.0053 0.0127 0.0008 0.0043 0.0174 0.0327 0.0001
Bud 0 0 0 0.0002 0 0 0 0 0 0 0.0001 0 0.0003 0.0001 0.0009 0.0013 0.004 0
Bud Neck 0 0 0 0.0005 0 0 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0012 0.0002 0
Bud Periphery 0 0 0 0.0008 0 0 0 0 0 0 0.0001 0 0.0002 0.0001 0.0003 0.0019 0.0051 0
Bud Site 0.0001 0 0 0.0001 0 0 0 0.0001 0.0001 0 0.0002 0 0.0004 0.0004 0.0002 0.0016 0.003 0
Cell Periphery 0.0002 0.0001 0.0002 0.0023 0.0001 0 0.0004 0.0004 0.001 0.0001 0.0007 0.0001 0.0002 0.0008 0.0002 0.0006 0.0003 0.0001
Cytoplasm 0.0006 0 0 0.0003 0 0 0 0 0 0 0.0002 0 0.0002 0.0002 0.0003 0.0014 0.0016 0.0026
Cytoplasmic Foci 0.0007 0 0 0.0006 0 0 0 0.0001 0.0007 0 0.0021 0.0001 0.0124 0.0049 0.0047 0.0693 0.0563 0.0001
Eisosomes 0.0013 0.0018 0.0015 0.0008 0.0015 0.0003 0.0034 0.0067 0.005 0.0004 0.0016 0.0015 0.0013 0.0008 0.0026 0.0003 0.0008 0.0002
Endoplasmic Reticulum 0.0003 0 0 0.0133 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0005 0.0003 0.0005 0
Endosome 0.0006 0.0001 0.0001 0.0187 0 0 0.0001 0.0001 0.0001 0 0.0029 0.0002 0.001 0.0001 0.0013 0.01 0.0032 0
Golgi 0.0005 0.0003 0.0007 0.0302 0 0.0002 0.0002 0.0008 0.0007 0.0003 0.0012 0.0005 0.0026 0.0002 0.0007 0.0218 0.0029 0
Lipid Particles 0.0051 0.0051 0.0018 0.0136 0.0012 0.0005 0.0035 0.008 0.0037 0.0041 0.0106 0.0045 0.0072 0.0067 0.0039 0.0385 0.0192 0.0011
Mitochondria 0.9733 0.9716 0.9866 0.8927 0.9887 0.9924 0.9786 0.9689 0.9689 0.9735 0.9586 0.9739 0.9402 0.9645 0.968 0.7811 0.8102 0.9783
None 0.0004 0 0 0.0002 0 0 0 0 0 0 0.0007 0 0.0012 0.0001 0.0008 0.0033 0.0124 0.0071
Nuclear Periphery 0.0028 0.0001 0 0.0017 0.0001 0 0.0003 0 0.0001 0 0.0013 0.0002 0.0002 0.0001 0.0007 0.001 0.0006 0.0001
Nucleolus 0.0015 0.0003 0.0009 0.0001 0.0001 0 0.0009 0.0012 0.0034 0.0001 0.0031 0.0001 0.0084 0.0037 0.0038 0.0104 0.0113 0.0008
Nucleus 0.002 0 0.0001 0.0003 0 0 0.0002 0.0001 0.0002 0 0.0005 0 0.0006 0.0002 0.0003 0.0008 0.0009 0.0001
Peroxisomes 0.0009 0.0018 0.0007 0.0004 0.0004 0.0002 0.0012 0.0009 0.0015 0.0002 0.0027 0.0004 0.0045 0.001 0.0019 0.0183 0.0246 0.0002
Punctate Nuclear 0.0011 0 0 0 0 0 0 0 0 0 0.0027 0.0002 0.0027 0.0003 0.0001 0.008 0.0077 0
Vacuole 0.0005 0.0008 0.0003 0.0064 0.0002 0.0001 0.0008 0.0007 0.0012 0.0006 0.0011 0.0002 0.0007 0.0014 0.0006 0.004 0.0006 0.0005
Vacuole Periphery 0.0066 0.0174 0.0066 0.013 0.0077 0.0062 0.0091 0.0114 0.0121 0.0206 0.0074 0.0127 0.0027 0.0136 0.0039 0.0076 0.0018 0.0086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 153.7883 132.6705 129.8362 108.7931 153.3795 234.3168 193.9241 244.0575 275.6823 200.23
Translational Efficiency 2.4501 2.2771 1.752 2.6575 1.5961 2.0328 1.9799 2.1378 1.7981 1.982

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘