Standard name
Description H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.08 0.06 0.07 0.08 0.09 0.17 0.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.12 0.07 0.35 0.21 0.27 0 0.14 0 0.08 0.08 0.06 0 0 0.1 0.6 0.6 0.41 0.16 0.21 0.1 0.18 0.19 0.16
Mitochondria 0.97 0.86 0.68 0.92 0.69 0.85 0.7 0.88 0.82 0.87 0.87 0.98 0.94 0.85 0.06 0.08 0.15 0.73 0.6 0.73 0.63 0.53 0.52
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.09 0.18 0.07 0.18 0.16 0.16 0.15 0.15 0.12 0.13 0.2 0.13 0.1 0.14 0.22 0.29 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.16 0.09 0.06 0.13 0.08 0.13 0 0.06 0 0.07 0.14 0.17 0.21 0.15 0.13 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 6 5 4 2 2 1 3 3 3 0 4 1 10 8 7 3 10 13 16 42 52
Bud 0 0 0 1 0 1 0 6 3 3 0 1 0 0 0 0 1 0 1 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1
Cell Periphery 0 2 4 2 0 2 2 2 0 1 2 1 0 1 5 1 1 0 0 1 0 1 0
Cytoplasm 0 3 3 2 5 16 18 18 13 14 5 0 3 1 3 2 1 0 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 1 6 3 1 0 1 4 2 6 0 2 0 0 1 0
Endosome 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4 10 44 31 56 9 44 7 28 20 11 5 10 25 71 54 35 9 32 16 32 47 41
Mitochondria 33 120 85 134 141 250 221 329 291 215 162 128 223 212 7 7 13 45 95 117 111 135 134
Nucleus 0 0 0 0 0 2 4 4 1 2 2 0 0 0 0 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 3 4 11 1 5 9 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 6 12 22 10 36 48 49 56 55 29 25 26 32 24 17 20 25 0 4 2 2 6 4
Peroxisomes 0 0 0 0 2 0 0 2 2 0 0 0 1 2 2 0 0 1 1 5 9 14 15
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 22 11 9 27 22 41 11 22 8 14 18 41 52 18 12 8 0 5 0 0 1 2
Unique Cell Count 34 140 125 146 205 293 314 376 356 246 187 131 238 249 118 90 85 62 159 160 179 253 256
Labelled Cell Count 45 172 176 194 272 357 385 448 422 306 236 180 314 319 137 107 102 62 159 160 179 253 256


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.3 77.3 64.9 61.0 57.3 34.5 40.7 30.0 30.4 29.2 26.3 100.1 92.9 103.1 56.3 50.4 53.1 78.3 83.0 82.8
Std Deviation (1e-4) 21.6 23.6 19.5 18.1 18.3 13.1 14.7 11.5 11.1 11.2 9.8 29.5 33.7 30.7 20.6 17.2 20.7 23.8 25.7 24.9
Intensity Change (Log2) -0.09 -0.18 -0.91 -0.67 -1.12 -1.09 -1.15 -1.3 0.62 0.52 0.67 -0.2 -0.37 -0.29 0.27 0.35 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 0 0 0 0 0 0 0 0 0 0 1.2 1.2 1.0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.4 1.5 1.2 0 1.4 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.6 -1.5 -9.0 -5.0 -10.7 -7.4 -6.5 -6.7 -6.4 -7.9 -5.9 3.9 3.6 0.9
Mitochondria 5.0 0.1 4.1 0.5 5.0 3.2 4.5 4.0 6.4 6.5 3.9 -10.0 -8.8 -7.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.7 0 -0.3 -0.5 -0.7 -0.6 -1.5 -1.0 0.5 -1.1 -2.2 -0.7 0.8 2.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 1.2 -0.4 1.2 -2.8 -1.0 -2.3 -0.4 1.2 2.2 2.9 1.6 1.1 0.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcv3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available