Standard name
Human Ortholog
Description Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.1 0.12 0.24 0.15 0.18 0.2 0.15 0.15 0.13 0.09 0.18 0.26 0.26 0.16 0.16 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.31 0.34 0.34 0.44 0.12 0.12 0.06 0 0 0 0 0.36 0.52 0.53 0.34 0.34 0.31 0.19 0.16 0.16 0.14 0.15 0.23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.11 0.07 0.17 0.13 0.56 0.46 0.56 0.75 0.8 0.77 0.84 0 0 0 0.18 0.08 0.07 0 0 0 0 0 0
Nucleus 0.23 0.26 0.09 0.06 0 0 0.05 0 0.05 0 0 0 0.09 0 0 0.1 0.14 0.15 0.07 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.27 0.15 0.25 0.29 0.09 0 0.05 0.07 0.07 0 0 0.1 0.21 0.16 0.11 0.2 0.19 0.2 0.3 0.32 0.13 0.15 0.09 0.11
Peroxisomes 0 0 0.05 0.16 0.1 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.24 0.35 0.56 0.36 0.34 0.25 0.32 0.3 0.15 0.13 0.19 0.14 0.49 0.38 0.35 0.53 0.5 0.56 0.31 0.27 0.51 0.5 0.5 0.43
Vac/Vac Membrane 0 0.1 0 0 0.08 0 0 0 0 0 0 0 0.05 0 0 0.06 0.09 0.11 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 3 1 1 2 2 4 16 10 9 9 5 8 0 1 1 1 1 1 1 3 1 1 3 2
Bud Neck 0 0 0 0 1 0 0 1 1 1 2 1 0 0 2 1 2 2 4 5 1 2 7 10
Bud Site 0 2 0 0 1 3 11 7 7 4 5 9 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 12 11 11 24 32 41 51 26 31 22 18 34 65 54 64 35 20 1 3 4 4 8 3
Endoplasmic Reticulum 1 1 0 0 0 2 0 0 2 0 2 2 1 1 2 10 10 4 0 0 0 1 3 0
Endosome 2 84 39 32 44 25 28 16 4 6 6 4 70 129 112 137 76 54 25 62 22 22 28 48
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0
Mitochondria 68 30 8 16 13 120 103 144 130 169 129 174 8 2 3 73 18 12 0 9 4 4 4 4
Nucleus 26 72 10 6 2 4 11 10 9 7 6 8 17 10 4 42 32 25 9 39 1 3 3 1
Nuclear Periphery 0 3 0 1 0 2 0 0 0 0 0 1 0 2 0 2 2 0 1 1 0 0 0 3
Nucleolus 31 42 29 28 9 9 12 17 12 7 5 20 40 41 24 81 42 34 39 122 18 23 17 23
Peroxisomes 1 1 6 15 10 14 21 5 3 7 7 1 0 4 6 3 0 1 0 1 0 0 0 1
SpindlePole 27 95 64 34 34 54 72 77 26 28 31 29 94 94 73 213 111 96 41 104 74 79 92 90
Vac/Vac Membrane 4 28 2 3 8 5 5 5 5 2 1 1 9 10 8 26 19 19 3 15 4 5 9 8
Unique Cell Count 114 272 115 95 100 216 224 255 174 210 167 206 193 249 210 405 222 172 134 380 144 158 185 210
Labelled Cell Count 167 371 170 149 148 274 320 343 234 271 221 276 273 359 289 653 349 269 134 380 144 158 185 210


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.9 5.1 5.0 4.6 3.7 3.1 3.6 3.6 2.8 3.1 2.8 5.1 5.0 4.8 5.7 6.8 6.8 4.6 4.7 5.6
Std Deviation (1e-4) 0.5 0.7 2.2 2.7 2.4 2.0 1.4 2.0 2.5 1.0 1.7 1.2 1.2 1.7 1.5 1.4 1.5 1.5 1.3 1.5 1.9
Intensity Change (Log2) -0.04 -0.14 -0.46 -0.72 -0.49 -0.51 -0.87 -0.69 -0.84 0.01 -0.02 -0.08 0.18 0.43 0.41 -0.15 -0.1 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.0 2.5 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0145 0.0003 0.0177 0.0002 0.0035 0.0017 0.0004 0.0002 0.0015 0.0359 0.0081 0.0006 0 0.0001 0.029 0.0154 0.0003 0.0057
Bud 0.0005 0.0002 0.0004 0.0002 0.0007 0.0003 0 0.0003 0.0004 0.0004 0.0063 0.0001 0 0.0002 0.0011 0.001 0.0003 0.0028
Bud Neck 0.0163 0.0119 0.0095 0.009 0.0483 0.0176 0.0013 0.0037 0.0091 0.0164 0.0192 0.0063 0.0035 0.0041 0.0089 0.051 0.0104 0.0166
Bud Periphery 0.0008 0.0005 0.0007 0.0005 0.0011 0.0008 0.0001 0.0003 0.0007 0.0005 0.0084 0.0002 0.0001 0.0002 0.0018 0.0028 0.0002 0.0145
Bud Site 0.0132 0.0077 0.0069 0.0045 0.0091 0.0041 0.0011 0.0199 0.0101 0.0043 0.003 0.001 0.0011 0.0054 0.0207 0.0085 0.0034 0.0049
Cell Periphery 0.0004 0.001 0.0014 0.0025 0.0005 0.0007 0.0003 0.0005 0.0016 0.0008 0.0003 0.0002 0 0.0004 0.0035 0.0038 0.0004 0.0005
Cytoplasm 0.0048 0.0426 0.0173 0.0149 0.0227 0.0114 0.1128 0.0166 0.0204 0.0028 0.0125 0.0165 0.0002 0.088 0.0351 0.0335 0.1004 0.0073
Cytoplasmic Foci 0.044 0.0497 0.0408 0.0382 0.0544 0.0615 0.1508 0.0512 0.0598 0.0955 0.0251 0.0406 0.0369 0.0348 0.0405 0.1112 0.1803 0.0425
Eisosomes 0.001 0.0001 0.0007 0.0003 0.0011 0.0002 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0 0 0.0006 0.0026 0 0.0001
Endoplasmic Reticulum 0.0012 0.0079 0.0057 0.0019 0.0021 0.0012 0.0058 0.001 0.0027 0.0052 0.0036 0.0018 0 0.0069 0.0044 0.0098 0.0057 0.001
Endosome 0.0227 0.0377 0.0257 0.0313 0.0229 0.0223 0.0275 0.0349 0.06 0.0505 0.0893 0.0258 0.0041 0.044 0.0317 0.067 0.0435 0.0215
Golgi 0.0106 0.0054 0.0093 0.0032 0.0049 0.0043 0.0056 0.0016 0.0137 0.0319 0.0281 0.0053 0.0047 0.0016 0.0036 0.0385 0.002 0.0036
Lipid Particles 0.0856 0.1216 0.1147 0.123 0.0897 0.0846 0.2093 0.0238 0.0806 0.1248 0.005 0.0727 0.0713 0.0316 0.0718 0.1837 0.1684 0.0655
Mitochondria 0.0053 0.0021 0.0196 0.0013 0.0018 0.0019 0.0008 0.0004 0.0103 0.0051 0.0286 0.004 0 0.0005 0.009 0.0434 0.0008 0.0014
None 0.0207 0.0872 0.0925 0.2567 0.1745 0.0805 0.0537 0.0783 0.1026 0.0018 0.0474 0.0603 0.0001 0.0191 0.1184 0.1494 0.0168 0.0388
Nuclear Periphery 0.0628 0.0263 0.0048 0.0019 0.0249 0.0074 0.0156 0.0033 0.0064 0.006 0.0056 0.0076 0.0009 0.0231 0.0153 0.0046 0.0082 0.0067
Nucleolus 0.2646 0.2483 0.3279 0.2577 0.0938 0.1859 0.0833 0.2843 0.2553 0.223 0.1211 0.1335 0.0531 0.2614 0.2915 0.1286 0.319 0.2441
Nucleus 0.0507 0.0677 0.1034 0.0433 0.0688 0.108 0.0346 0.1073 0.1578 0.076 0.2568 0.0569 0.0041 0.2235 0.1312 0.0335 0.0758 0.0459
Peroxisomes 0.0252 0.0206 0.0155 0.0059 0.0066 0.0122 0.0294 0.0017 0.0248 0.0358 0.0023 0.0049 0.0049 0.0015 0.0045 0.0495 0.0053 0.0087
Punctate Nuclear 0.3504 0.2425 0.1647 0.1541 0.3638 0.3848 0.2622 0.36 0.1511 0.2654 0.3119 0.5578 0.8147 0.215 0.1295 0.0225 0.0469 0.4585
Vacuole 0.003 0.0161 0.0198 0.0479 0.0039 0.0077 0.0048 0.0102 0.0278 0.0171 0.0092 0.0033 0.0002 0.0356 0.0456 0.0344 0.0112 0.0086
Vacuole Periphery 0.0019 0.0026 0.001 0.0012 0.0008 0.001 0.0005 0.0005 0.0029 0.0008 0.0082 0.0006 0 0.0029 0.0024 0.0052 0.0007 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.5686 10.2149 9.4749 9.6885 9.2596 13.5619 11.1352 8.0601 5.913 13.1374
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
691 300 381 1720 2368 926 243 288 3059 1226 624 2008

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.87 655.37 877.03 921.28 719.70 729.54 987.03 929.45 704.38 711.39 919.87 922.45
Standard Deviation 67.79 120.82 101.70 132.39 78.32 93.34 105.97 134.91 81.18 105.68 116.47 132.79
Intensity Change Log 2 0.007725 0.428042 0.499055 0.019591 0.455698 0.368982 0.013964 0.442620 0.432266

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.001035 0.001298 0.001216 0.000096 0.000550 0.001331 0.001700 0.000085 0.000669 0.001311 0.001285
Bud Neck 0.003121 0.016121 0.014364 0.047257 0.007699 0.013672 0.007725 0.018178 0.006665 0.014271 0.011778 0.043086
Bud Site 0.000289 0.010451 0.003103 0.017975 0.000783 0.005282 0.003071 0.009001 0.000671 0.006547 0.003091 0.016688
Cell Periphery 0.000100 0.000221 0.000091 0.000114 0.000071 0.000209 0.000110 0.000147 0.000077 0.000212 0.000099 0.000119
Cytoplasm 0.042616 0.128191* 0.015493 0.019099 0.057737 0.089074 0.061232 0.078533 0.054321 0.098646 0.033304 0.027623
Cytoplasmic Foci 0.042943 0.147558 0.004210 0.003415 0.058202 0.113684 0.006251 0.017362 0.054755 0.121973 0.005005 0.005415
Eisosomes 0.000094 0.000195 0.000121 0.000065 0.000090 0.000134 0.000177 0.000063 0.000091 0.000149 0.000143 0.000065
Endoplasmic Reticulum 0.000351 0.003372 0.005858 0.006036 0.001020 0.001495 0.007534 0.005722 0.000869 0.001954 0.006511 0.005991
Endosome 0.004516 0.034098 0.002275 0.006807 0.009277 0.030665 0.003465 0.031140 0.008202 0.031505 0.002739 0.010297
Golgi 0.001487 0.013050 0.000665 0.003566 0.002504 0.013930 0.000636 0.014868 0.002274 0.013715 0.000654 0.005187
Lipid Particles 0.011220 0.058290 0.002198 0.000924 0.033380 0.043757 0.002912 0.000994 0.028374 0.047313 0.002476 0.000934
Mitochondria 0.004599 0.032694 0.001344 0.006543 0.011037 0.024721 0.000948 0.003839 0.009583 0.026672 0.001190 0.006155
Mitotic Spindle 0.003312 0.096774 0.029268 0.290830* 0.034248 0.095249 0.015475 0.327127* 0.027260 0.095622 0.023897 0.296036*
None 0.030378 0.038811 0.006075 0.006099 0.013422 0.020382 0.011837 0.019148 0.017253 0.024892 0.008319 0.007971
Nuclear Periphery 0.001157 0.003108 0.001663 0.006155 0.005019 0.004333 0.001573 0.002098 0.004147 0.004033 0.001628 0.005573
Nuclear Periphery Foci 0.008954 0.031609 0.012515 0.017958 0.037881 0.021342 0.021288 0.009777 0.031346 0.023855 0.015932 0.016785
Nucleolus 0.611065* 0.156034 0.324145* 0.265586* 0.516317* 0.302549* 0.348733* 0.172313 0.537720* 0.266697* 0.333720* 0.252208*
Nucleus 0.226213* 0.169647* 0.563261* 0.273963* 0.201023* 0.178883* 0.467716* 0.254978* 0.206714* 0.176623* 0.526054* 0.271240*
Peroxisomes 0.005653 0.033672 0.002475 0.002134 0.007612 0.026009 0.003692 0.001529 0.007169 0.027884 0.002949 0.002047
Vacuole 0.001320 0.021570 0.009037 0.017458 0.002133 0.011928 0.033890 0.026494 0.001949 0.014288 0.018715 0.018754
Vacuole Periphery 0.000567 0.003499 0.000543 0.006800 0.000446 0.002151 0.000401 0.004989 0.000474 0.002481 0.000488 0.006541

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.77 -12.54 -17.69 -2.48 2.83 -3.02 -11.46 -7.99 -5.49 2.21 -5.22 -16.76 -19.00 -7.17 4.55
Bud Neck -7.46 -12.83 -27.74 -17.00 -12.91 -4.39 -7.86 -9.45 -7.35 -4.41 -6.79 -13.74 -27.77 -20.73 -14.63
Bud Site -4.74 -9.41 -15.95 -3.46 -7.79 -3.21 -8.55 -9.80 -6.72 -2.51 -5.03 -12.09 -17.89 -8.47 -6.45
Cell Periphery -5.76 -1.23 -1.45 5.91 -0.08 -1.68 -8.02 -5.25 0.50 1.77 -2.35 -7.53 -7.70 1.38 2.06
Cytoplasm -6.70 4.71 3.53 8.40 -2.64 -5.28 1.52 -1.06 2.27 -1.89 -8.01 6.00 7.05 11.97 -0.85
Cytoplasmic Foci -6.38 11.27 11.85 14.06 1.69 -6.93 19.09 19.93 19.68 0.55 -9.23 23.73 26.15 25.17 2.41
Eisosomes -8.07 -4.06 7.80 11.40 8.11 -9.04 -7.96 -0.67 4.54 7.02 -11.99 -9.06 8.35 16.25 11.56
Endoplasmic Reticulum -6.57 -14.32 -20.60 -6.43 1.85 -5.22 -12.90 -9.92 -8.92 3.71 -8.22 -18.63 -21.78 -15.94 4.54
Endosome -7.42 0.81 -0.79 7.37 -2.54 -8.35 2.48 0.04 5.65 -0.95 -10.86 6.05 5.49 12.83 -0.48
Golgi -5.23 3.20 1.61 5.74 -1.02 -5.69 5.25 -0.52 3.95 -1.60 -7.21 7.67 2.42 7.76 -1.34
Lipid Particles -6.17 5.71 6.47 7.82 4.19 -2.53 14.50 16.50 13.17 4.61 -5.28 16.20 17.62 15.31 6.42
Mitochondria -5.47 2.28 -1.19 5.30 -7.40 -5.80 13.68 8.98 9.14 -3.22 -7.83 13.38 6.16 9.93 -8.04
Mitotic Spindle -8.15 -5.52 -31.67* -11.18 -20.25 -7.88 -3.68 -10.85 -7.63 -7.53 -10.69 -4.42 -30.29 -17.56 -20.81
None -0.87 8.14 8.28 5.05 0.12 -2.70 4.51 0.89 2.25 -1.13 -2.53 10.68 11.33 8.33 0.15
Nuclear Periphery -4.50 -4.69 -13.20 -7.61 -10.52 0.21 3.05 -0.69 -0.79 -2.39 -0.86 2.85 -8.03 -6.56 -10.67
Nuclear Periphery Foci -3.32 0.45 -0.64 3.24 -1.31 5.20 1.57 6.91 2.51 3.62 2.69 4.51 7.34 3.43 1.46
Nucleolus 23.34* 15.20 24.76* -5.62 4.43 15.99* 12.50 23.99* 9.82 5.48 23.95* 18.68 34.56* 3.93 5.07
Nucleus 0.76 -18.91 -8.76 -8.06 14.19 -0.20 -12.08 -9.12 -8.74 2.42 0.17 -23.68 -15.81 -13.80 13.51
Peroxisomes -7.53 5.13 8.03 9.62 4.47 -9.26 3.48 10.85 13.03 3.76 -11.71 7.93 16.76 16.58 6.24
Vacuole -5.72 -5.79 -13.62 -1.12 -3.53 -6.57 -6.76 -6.90 -5.65 0.94 -8.67 -8.11 -14.84 -7.12 1.17
Vacuole Periphery -5.07 -1.76 -2.85 1.00 -2.34 -8.10 -7.24 -2.88 0.19 -1.95 -9.35 -5.08 -3.34 -0.17 -2.57