TheCellVision

RRN7

Standard name	RRN7 ORF YJL025W
Description	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p

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Scale images

rrn7-5001

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0.07	0	0.05	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05
Bud Site	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0	0	0.1	0.12	0.24	0.15	0.18	0.2	0.15	0.15	0.13	0.09	0.18	0.26	0.26	0.16	0.16	0.12	0	0	0	0	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0
Endosome	0	0.31	0.34	0.34	0.44	0.12	0.12	0.06	0	0	0	0	0.36	0.52	0.53	0.34	0.34	0.31	0.19	0.16	0.16	0.14	0.15	0.23
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0.6	0.11	0.07	0.17	0.13	0.56	0.46	0.56	0.75	0.8	0.77	0.84	0	0	0	0.18	0.08	0.07	0	0	0	0	0	0
Nucleus	0.23	0.26	0.09	0.06	0	0	0.05	0	0.05	0	0	0	0.09	0	0	0.1	0.14	0.15	0.07	0.1	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0.27	0.15	0.25	0.29	0.09	0	0.05	0.07	0.07	0	0	0.1	0.21	0.16	0.11	0.2	0.19	0.2	0.3	0.32	0.13	0.15	0.09	0.11
Peroxisomes	0	0	0.05	0.16	0.1	0.06	0.09	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0.24	0.35	0.56	0.36	0.34	0.25	0.32	0.3	0.15	0.13	0.19	0.14	0.49	0.38	0.35	0.53	0.5	0.56	0.31	0.27	0.51	0.5	0.5	0.43
Vac/Vac Membrane	0	0.1	0	0	0.08	0	0	0	0	0	0	0	0.05	0	0	0.06	0.09	0.11	0	0	0	0	0.05	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	3	1	1	2	2	4	16	10	9	9	5	8	0	1	1	1	1	1	1	3	1	1	3	2
Bud Neck	0	0	0	0	1	0	0	1	1	1	2	1	0	0	2	1	2	2	4	5	1	2	7	10
Bud Site	0	2	0	0	1	3	11	7	7	4	5	9	0	0	0	0	0	1	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	4	12	11	11	24	32	41	51	26	31	22	18	34	65	54	64	35	20	1	3	4	4	8	3
Endoplasmic Reticulum	1	1	0	0	0	2	0	0	2	0	2	2	1	1	2	10	10	4	0	0	0	1	3	0
Endosome	2	84	39	32	44	25	28	16	4	6	6	4	70	129	112	137	76	54	25	62	22	22	28	48
Golgi	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	1	0	0
Mitochondria	68	30	8	16	13	120	103	144	130	169	129	174	8	2	3	73	18	12	0	9	4	4	4	4
Nucleus	26	72	10	6	2	4	11	10	9	7	6	8	17	10	4	42	32	25	9	39	1	3	3	1
Nuclear Periphery	0	3	0	1	0	2	0	0	0	0	0	1	0	2	0	2	2	0	1	1	0	0	0	3
Nucleolus	31	42	29	28	9	9	12	17	12	7	5	20	40	41	24	81	42	34	39	122	18	23	17	23
Peroxisomes	1	1	6	15	10	14	21	5	3	7	7	1	0	4	6	3	0	1	0	1	0	0	0	1
SpindlePole	27	95	64	34	34	54	72	77	26	28	31	29	94	94	73	213	111	96	41	104	74	79	92	90
Vac/Vac Membrane	4	28	2	3	8	5	5	5	5	2	1	1	9	10	8	26	19	19	3	15	4	5	9	8
Unique Cell Count	114	272	115	95	100	216	224	255	174	210	167	206	193	249	210	405	222	172	134	380	144	158	185	210
Labelled Cell Count	167	371	170	149	148	274	320	343	234	271	221	276	273	359	289	653	349	269	134	380	144	158	185	210

Yeast GFP Assignment

Nucleolus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	3.8	4.9	5.1	5.0	4.6	3.7	3.1	3.6	3.6	2.8	3.1	2.8	5.1	5.0	4.8	5.7	6.8	6.8	4.6	4.7	5.6
Std Deviation (1e^-4)	0.5	0.7	2.2	2.7	2.4	2.0	1.4	2.0	2.5	1.0	1.7	1.2	1.2	1.7	1.5	1.4	1.5	1.5	1.3	1.5	1.9
Intensity Change (Log₂)	–	–	–	-0.04	-0.14	-0.46	-0.72	-0.49	-0.51	-0.87	-0.69	-0.84	0.01	-0.02	-0.08	0.18	0.43	0.41	-0.15	-0.1	0.13

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	2.0	2.5	3.0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Actin	0.0145	0.0003	0.0177	0.0002	0.0035	0.0017	0.0004	0.0002	0.0015	0.0359	0.0081	0.0006	0	0.0001	0.029	0.0154	0.0003	0.0057
Bud	0.0005	0.0002	0.0004	0.0002	0.0007	0.0003	0	0.0003	0.0004	0.0004	0.0063	0.0001	0	0.0002	0.0011	0.001	0.0003	0.0028
Bud Neck	0.0163	0.0119	0.0095	0.009	0.0483	0.0176	0.0013	0.0037	0.0091	0.0164	0.0192	0.0063	0.0035	0.0041	0.0089	0.051	0.0104	0.0166
Bud Periphery	0.0008	0.0005	0.0007	0.0005	0.0011	0.0008	0.0001	0.0003	0.0007	0.0005	0.0084	0.0002	0.0001	0.0002	0.0018	0.0028	0.0002	0.0145
Bud Site	0.0132	0.0077	0.0069	0.0045	0.0091	0.0041	0.0011	0.0199	0.0101	0.0043	0.003	0.001	0.0011	0.0054	0.0207	0.0085	0.0034	0.0049
Cell Periphery	0.0004	0.001	0.0014	0.0025	0.0005	0.0007	0.0003	0.0005	0.0016	0.0008	0.0003	0.0002	0	0.0004	0.0035	0.0038	0.0004	0.0005
Cytoplasm	0.0048	0.0426	0.0173	0.0149	0.0227	0.0114	0.1128	0.0166	0.0204	0.0028	0.0125	0.0165	0.0002	0.088	0.0351	0.0335	0.1004	0.0073
Cytoplasmic Foci	0.044	0.0497	0.0408	0.0382	0.0544	0.0615	0.1508	0.0512	0.0598	0.0955	0.0251	0.0406	0.0369	0.0348	0.0405	0.1112	0.1803	0.0425
Eisosomes	0.001	0.0001	0.0007	0.0003	0.0011	0.0002	0.0001	0.0001	0.0006	0.0001	0.0001	0.0001	0	0	0.0006	0.0026	0	0.0001
Endoplasmic Reticulum	0.0012	0.0079	0.0057	0.0019	0.0021	0.0012	0.0058	0.001	0.0027	0.0052	0.0036	0.0018	0	0.0069	0.0044	0.0098	0.0057	0.001
Endosome	0.0227	0.0377	0.0257	0.0313	0.0229	0.0223	0.0275	0.0349	0.06	0.0505	0.0893	0.0258	0.0041	0.044	0.0317	0.067	0.0435	0.0215
Golgi	0.0106	0.0054	0.0093	0.0032	0.0049	0.0043	0.0056	0.0016	0.0137	0.0319	0.0281	0.0053	0.0047	0.0016	0.0036	0.0385	0.002	0.0036
Lipid Particles	0.0856	0.1216	0.1147	0.123	0.0897	0.0846	0.2093	0.0238	0.0806	0.1248	0.005	0.0727	0.0713	0.0316	0.0718	0.1837	0.1684	0.0655
Mitochondria	0.0053	0.0021	0.0196	0.0013	0.0018	0.0019	0.0008	0.0004	0.0103	0.0051	0.0286	0.004	0	0.0005	0.009	0.0434	0.0008	0.0014
None	0.0207	0.0872	0.0925	0.2567	0.1745	0.0805	0.0537	0.0783	0.1026	0.0018	0.0474	0.0603	0.0001	0.0191	0.1184	0.1494	0.0168	0.0388
Nuclear Periphery	0.0628	0.0263	0.0048	0.0019	0.0249	0.0074	0.0156	0.0033	0.0064	0.006	0.0056	0.0076	0.0009	0.0231	0.0153	0.0046	0.0082	0.0067
Nucleolus	0.2646	0.2483	0.3279	0.2577	0.0938	0.1859	0.0833	0.2843	0.2553	0.223	0.1211	0.1335	0.0531	0.2614	0.2915	0.1286	0.319	0.2441
Nucleus	0.0507	0.0677	0.1034	0.0433	0.0688	0.108	0.0346	0.1073	0.1578	0.076	0.2568	0.0569	0.0041	0.2235	0.1312	0.0335	0.0758	0.0459
Peroxisomes	0.0252	0.0206	0.0155	0.0059	0.0066	0.0122	0.0294	0.0017	0.0248	0.0358	0.0023	0.0049	0.0049	0.0015	0.0045	0.0495	0.0053	0.0087
Punctate Nuclear	0.3504	0.2425	0.1647	0.1541	0.3638	0.3848	0.2622	0.36	0.1511	0.2654	0.3119	0.5578	0.8147	0.215	0.1295	0.0225	0.0469	0.4585
Vacuole	0.003	0.0161	0.0198	0.0479	0.0039	0.0077	0.0048	0.0102	0.0278	0.0171	0.0092	0.0033	0.0002	0.0356	0.0456	0.0344	0.0112	0.0086
Vacuole Periphery	0.0019	0.0026	0.001	0.0012	0.0008	0.001	0.0005	0.0005	0.0029	0.0008	0.0082	0.0006	0	0.0029	0.0024	0.0052	0.0007	0.0007

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	18.5686	10.2149	9.4749	9.6885	9.2596	13.5619	11.1352	8.0601	5.913	13.1374
Translational Efficiency	–	–	–	–	–	–	–	–	–	–

Hit Data

Dataset	Hit
Protein Abundance	–
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	–