Standard name | |
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Description | Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 |
Bud | 0 | 1 | 0 | 0 | 2 | 3 | 8 | – | 12 | 19 | 9 | 8 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 2 | 4 | 16 | 14 |
Bud Neck | 0 | 1 | 6 | 2 | 7 | 2 | 9 | – | 2 | 10 | 6 | 8 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | 3 | 9 | 18 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 4 | 3 | – | 18 | 15 | 8 | 15 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 13 | 17 | 19 | 51 | 41 | 55 | – | 36 | 52 | 31 | 38 | 19 | 60 | 70 | 26 | 12 | 16 | 3 | 16 | 5 | 3 | 5 | 6 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 5 | 4 | 0 | 0 | 1 | 1 | 2 | 2 |
Endosome | 1 | 1 | 1 | 0 | 4 | 4 | 3 | – | 5 | 0 | 0 | 3 | 1 | 12 | 23 | 11 | 7 | 7 | 11 | 1 | 3 | 7 | 26 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 3 | 0 | 3 | 0 |
Mitochondria | 53 | 135 | 17 | 37 | 46 | 122 | 163 | – | 261 | 295 | 199 | 201 | 95 | 27 | 45 | 18 | 8 | 16 | 7 | 9 | 8 | 8 | 18 | 21 |
Nucleus | 145 | 74 | 173 | 128 | 164 | 141 | 185 | – | 186 | 182 | 114 | 179 | 253 | 231 | 244 | 114 | 97 | 90 | 130 | 94 | 144 | 97 | 179 | 139 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 |
Nucleolus | 6 | 6 | 0 | 1 | 1 | 1 | 3 | – | 6 | 9 | 4 | 4 | 6 | 8 | 23 | 6 | 1 | 4 | 37 | 14 | 46 | 33 | 77 | 67 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 8 | 4 | 43 | 23 | 34 | 15 | 30 | – | 21 | 20 | 11 | 24 | 13 | 11 | 11 | 15 | 5 | 8 | 17 | 19 | 18 | 19 | 48 | 54 |
Vac/Vac Membrane | 2 | 6 | 3 | 2 | 11 | 4 | 7 | – | 11 | 13 | 5 | 8 | 3 | 11 | 8 | 21 | 11 | 9 | 19 | 40 | 41 | 45 | 71 | 84 |
Unique Cell Count | 189 | 188 | 221 | 174 | 279 | 259 | 364 | 385 | 449 | 267 | 343 | 337 | 337 | 380 | 182 | 129 | 131 | 242 | 208 | 293 | 231 | 476 | 448 | |
Labelled Cell Count | 225 | 244 | 260 | 212 | 321 | 337 | 466 | 559 | 616 | 391 | 489 | 394 | 361 | 433 | 220 | 148 | 156 | 242 | 208 | 293 | 231 | 476 | 448 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 3.4 | 4.9 | 4.7 | 5.0 | 4.2 | 4.2 | – | 3.9 | 3.7 | 3.4 | 3.8 | 4.3 | 4.4 | 4.7 | 6.6 | 6.8 | 6.6 | 4.1 | 4.3 | 4.6 |
Std Deviation (1e-4) | 0.6 | 0.6 | 1.2 | 1.6 | 1.4 | 1.7 | 1.2 | – | 1.6 | 1.4 | 1.3 | 0.9 | 0.8 | 0.9 | 4.4 | 1.6 | 1.5 | 1.5 | 1.2 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.02 | -0.24 | -0.25 | – | -0.36 | -0.42 | -0.52 | -0.37 | -0.19 | -0.18 | -0.06 | 0.41 | 0.46 | 0.43 | -0.26 | -0.21 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.3 | 3.3 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1972 | -0.4278 | -0.815 | -0.5875 | 0.189 | -0.7521 | -0.3745 | -0.2213 | -0.2747 | -0.5055 | -0.4192 | -0.4983 | -0.5947 | -0.3201 | -0.7614 | -0.7331 | -0.6871 | -0.8668 |
Actin | 0.0258 | 0.0007 | 0.0017 | 0.0292 | 0.0615 | 0.0019 | 0.0659 | 0.004 | 0.0217 | 0.0119 | 0.0016 | 0.0048 | 0.0087 | 0.0002 | 0.0095 | 0.0002 | 0.0004 | 0.0173 |
Bud | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0021 | 0.0001 | 0.0014 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0001 |
Bud Neck | 0.0013 | 0.0025 | 0.0014 | 0.0011 | 0.0022 | 0.0253 | 0.0217 | 0.001 | 0.0065 | 0.0017 | 0.0023 | 0.0032 | 0.0013 | 0.0011 | 0.0008 | 0.0009 | 0.0026 | 0.0047 |
Bud Periphery | 0.0002 | 0.0006 | 0.0002 | 0.0015 | 0.0049 | 0.0003 | 0.0042 | 0.0001 | 0.0009 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0002 |
Bud Site | 0.0015 | 0.0079 | 0.0016 | 0.0003 | 0.0061 | 0.0005 | 0.0089 | 0.0028 | 0.0067 | 0.0055 | 0.0004 | 0.0006 | 0.001 | 0.0014 | 0.0017 | 0.0003 | 0.0016 | 0.0004 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.0252 | 0.0556 | 0.0288 | 0.004 | 0.0249 | 0.0319 | 0.0347 | 0.0529 | 0.0212 | 0.0365 | 0.0168 | 0.0167 | 0.0445 | 0.0644 | 0.0241 | 0.0168 | 0.0345 | 0.0125 |
Cytoplasmic Foci | 0.0152 | 0.0052 | 0.0102 | 0.0009 | 0.0583 | 0.0031 | 0.0138 | 0.0123 | 0.0083 | 0.0382 | 0.0056 | 0.0104 | 0.0135 | 0.0026 | 0.0034 | 0.0008 | 0.0073 | 0.0027 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0013 |
Endoplasmic Reticulum | 0.0023 | 0.0028 | 0.0015 | 0.0094 | 0.0044 | 0.0015 | 0.0065 | 0.0014 | 0.0011 | 0.0013 | 0.0013 | 0.0006 | 0.0068 | 0.0018 | 0.0015 | 0.0006 | 0.0021 | 0.0018 |
Endosome | 0.0177 | 0.0046 | 0.0093 | 0.0273 | 0.0322 | 0.0068 | 0.0298 | 0.0055 | 0.0088 | 0.0173 | 0.0468 | 0.0062 | 0.0324 | 0.0024 | 0.0049 | 0.0008 | 0.0437 | 0.0025 |
Golgi | 0.0042 | 0.0004 | 0.0011 | 0.009 | 0.0161 | 0.0025 | 0.0049 | 0.0032 | 0.0088 | 0.0208 | 0.0353 | 0.0044 | 0.0316 | 0.0001 | 0.0023 | 0 | 0.0012 | 0.0038 |
Lipid Particles | 0.0273 | 0.0016 | 0.0091 | 0.003 | 0.0599 | 0.0107 | 0.0043 | 0.0007 | 0.0057 | 0.0073 | 0.0166 | 0.0043 | 0.0363 | 0.0013 | 0.0026 | 0.0001 | 0.0008 | 0.0083 |
Mitochondria | 0.0025 | 0.0008 | 0.0018 | 0.0052 | 0.0772 | 0.0009 | 0.0041 | 0.001 | 0.026 | 0.0014 | 0.018 | 0.0043 | 0.0068 | 0.0005 | 0.0167 | 0.0004 | 0.0016 | 0.0018 |
None | 0.1525 | 0.1159 | 0.09 | 0.0015 | 0.1003 | 0.0564 | 0.0294 | 0.0797 | 0.0283 | 0.0433 | 0.0659 | 0.014 | 0.043 | 0.092 | 0.013 | 0.0157 | 0.0058 | 0.0261 |
Nuclear Periphery | 0.0336 | 0.0181 | 0.0207 | 0.0307 | 0.0488 | 0.0222 | 0.0336 | 0.0145 | 0.0214 | 0.0208 | 0.0442 | 0.0075 | 0.0381 | 0.0167 | 0.0163 | 0.012 | 0.0263 | 0.011 |
Nucleolus | 0.0124 | 0.0144 | 0.0189 | 0.0084 | 0.0077 | 0.0325 | 0.0115 | 0.0178 | 0.0179 | 0.0036 | 0.0074 | 0.0197 | 0.0135 | 0.0122 | 0.0152 | 0.0074 | 0.0185 | 0.0264 |
Nucleus | 0.5806 | 0.6535 | 0.763 | 0.8542 | 0.3748 | 0.6969 | 0.6553 | 0.7239 | 0.764 | 0.7612 | 0.6669 | 0.8365 | 0.6471 | 0.7499 | 0.8086 | 0.8911 | 0.7745 | 0.7848 |
Peroxisomes | 0.0118 | 0.0009 | 0.0024 | 0.0004 | 0.0302 | 0.0008 | 0.0012 | 0.0057 | 0.0055 | 0.0122 | 0.0015 | 0.0131 | 0.0028 | 0.0001 | 0.0031 | 0 | 0.0006 | 0.0064 |
Punctate Nuclear | 0.0823 | 0.1068 | 0.0356 | 0.0087 | 0.0665 | 0.1043 | 0.0606 | 0.0717 | 0.0437 | 0.0156 | 0.0614 | 0.0515 | 0.0671 | 0.0514 | 0.07 | 0.052 | 0.0566 | 0.0869 |
Vacuole | 0.0016 | 0.0064 | 0.0017 | 0.0017 | 0.0105 | 0.0008 | 0.0043 | 0.0013 | 0.0015 | 0.0005 | 0.0048 | 0.0006 | 0.0028 | 0.0014 | 0.0035 | 0.0004 | 0.0171 | 0.0006 |
Vacuole Periphery | 0.0014 | 0.0006 | 0.0005 | 0.0025 | 0.0101 | 0.0006 | 0.0022 | 0.0002 | 0.001 | 0.0003 | 0.0026 | 0.0004 | 0.0017 | 0.0003 | 0.0012 | 0.0001 | 0.0037 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.0309 | 5.2677 | 6.189 | 6.6176 | 11.7785 | 4.4171 | 7.7673 | 5.542 | 6.8803 | 11.7743 |
Translational Efficiency | 0.4759 | 1.0179 | 0.3896 | 0.4142 | 0.4596 | 1.0949 | 0.5411 | 0.476 | 0.4253 | 0.3791 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |