Standard name
Description Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.08 0.11 0.18 0.16 0.15 – 0.09 0.12 0.12 0.11 0.06 0.18 0.18 0.14 0.09 0.12 0 0.08 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.06 0.05 0.05 0.05 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.72 0.08 0.21 0.16 0.47 0.45 – 0.68 0.66 0.75 0.59 0.28 0.08 0.12 0.1 0.06 0.12 0 0 0 0 0 0.05
Nucleus 0.77 0.39 0.78 0.74 0.59 0.54 0.51 – 0.48 0.41 0.43 0.52 0.75 0.69 0.64 0.63 0.75 0.69 0.54 0.45 0.49 0.42 0.38 0.31
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0.16 0.07 0.16 0.15 0.16 0.15
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.19 0.13 0.12 0.06 0.08 – 0.05 0 0 0.07 0 0 0 0.08 0 0.06 0.07 0.1 0.06 0.08 0.1 0.12
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.09 0.07 0.08 0.2 0.14 0.2 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 3
Bud 0 1 0 0 2 3 8 – 12 19 9 8 1 0 0 1 1 0 3 1 2 4 16 14
Bud Neck 0 1 6 2 7 2 9 – 2 10 6 8 1 0 0 0 1 0 2 2 3 3 9 18
Bud Site 0 1 0 0 0 4 3 – 18 15 8 15 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 7 13 17 19 51 41 55 – 36 52 31 38 19 60 70 26 12 16 3 16 5 3 5 6
Endoplasmic Reticulum 2 1 0 0 0 0 0 – 1 0 0 0 0 1 2 4 5 4 0 0 1 1 2 2
Endosome 1 1 1 0 4 4 3 – 5 0 0 3 1 12 23 11 7 7 11 1 3 7 26 18
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 3 0 2 0 0 3 0 3 0
Mitochondria 53 135 17 37 46 122 163 – 261 295 199 201 95 27 45 18 8 16 7 9 8 8 18 21
Nucleus 145 74 173 128 164 141 185 – 186 182 114 179 253 231 244 114 97 90 130 94 144 97 179 139
Nuclear Periphery 1 1 0 0 0 0 0 – 0 0 4 0 0 0 1 1 0 0 1 0 0 0 3 2
Nucleolus 6 6 0 1 1 1 3 – 6 9 4 4 6 8 23 6 1 4 37 14 46 33 77 67
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 5 0 0 0 0 0 0 0 1 0
SpindlePole 8 4 43 23 34 15 30 – 21 20 11 24 13 11 11 15 5 8 17 19 18 19 48 54
Vac/Vac Membrane 2 6 3 2 11 4 7 – 11 13 5 8 3 11 8 21 11 9 19 40 41 45 71 84
Unique Cell Count 189 188 221 174 279 259 364 385 449 267 343 337 337 380 182 129 131 242 208 293 231 476 448
Labelled Cell Count 225 244 260 212 321 337 466 559 616 391 489 394 361 433 220 148 156 242 208 293 231 476 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.4 4.9 4.7 5.0 4.2 4.2 – 3.9 3.7 3.4 3.8 4.3 4.4 4.7 6.6 6.8 6.6 4.1 4.3 4.6
Std Deviation (1e-4) 0.6 0.6 1.2 1.6 1.4 1.7 1.2 – 1.6 1.4 1.3 0.9 0.8 0.9 4.4 1.6 1.5 1.5 1.2 1.0 1.3
Intensity Change (Log2) – – – -0.08 0.02 -0.24 -0.25 – -0.36 -0.42 -0.52 -0.37 -0.19 -0.18 -0.06 0.41 0.46 0.43 -0.26 -0.21 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 4.3 3.3 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1972 -0.4278 -0.815 -0.5875 0.189 -0.7521 -0.3745 -0.2213 -0.2747 -0.5055 -0.4192 -0.4983 -0.5947 -0.3201 -0.7614 -0.7331 -0.6871 -0.8668
Actin 0.0258 0.0007 0.0017 0.0292 0.0615 0.0019 0.0659 0.004 0.0217 0.0119 0.0016 0.0048 0.0087 0.0002 0.0095 0.0002 0.0004 0.0173
Bud 0.0002 0.0004 0.0002 0.0005 0.0021 0.0001 0.0014 0.0002 0.0007 0.0003 0.0002 0.0003 0.0002 0.0002 0.0004 0.0001 0.0006 0.0001
Bud Neck 0.0013 0.0025 0.0014 0.0011 0.0022 0.0253 0.0217 0.001 0.0065 0.0017 0.0023 0.0032 0.0013 0.0011 0.0008 0.0009 0.0026 0.0047
Bud Periphery 0.0002 0.0006 0.0002 0.0015 0.0049 0.0003 0.0042 0.0001 0.0009 0.0002 0.0003 0.0008 0.0005 0.0001 0.0009 0.0001 0.0005 0.0002
Bud Site 0.0015 0.0079 0.0016 0.0003 0.0061 0.0005 0.0089 0.0028 0.0067 0.0055 0.0004 0.0006 0.001 0.0014 0.0017 0.0003 0.0016 0.0004
Cell Periphery 0.0001 0.0003 0.0001 0.0001 0.0006 0.0001 0.0011 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0002
Cytoplasm 0.0252 0.0556 0.0288 0.004 0.0249 0.0319 0.0347 0.0529 0.0212 0.0365 0.0168 0.0167 0.0445 0.0644 0.0241 0.0168 0.0345 0.0125
Cytoplasmic Foci 0.0152 0.0052 0.0102 0.0009 0.0583 0.0031 0.0138 0.0123 0.0083 0.0382 0.0056 0.0104 0.0135 0.0026 0.0034 0.0008 0.0073 0.0027
Eisosomes 0.0002 0.0001 0.0001 0.0002 0.0007 0.0001 0.0005 0 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0 0.0013
Endoplasmic Reticulum 0.0023 0.0028 0.0015 0.0094 0.0044 0.0015 0.0065 0.0014 0.0011 0.0013 0.0013 0.0006 0.0068 0.0018 0.0015 0.0006 0.0021 0.0018
Endosome 0.0177 0.0046 0.0093 0.0273 0.0322 0.0068 0.0298 0.0055 0.0088 0.0173 0.0468 0.0062 0.0324 0.0024 0.0049 0.0008 0.0437 0.0025
Golgi 0.0042 0.0004 0.0011 0.009 0.0161 0.0025 0.0049 0.0032 0.0088 0.0208 0.0353 0.0044 0.0316 0.0001 0.0023 0 0.0012 0.0038
Lipid Particles 0.0273 0.0016 0.0091 0.003 0.0599 0.0107 0.0043 0.0007 0.0057 0.0073 0.0166 0.0043 0.0363 0.0013 0.0026 0.0001 0.0008 0.0083
Mitochondria 0.0025 0.0008 0.0018 0.0052 0.0772 0.0009 0.0041 0.001 0.026 0.0014 0.018 0.0043 0.0068 0.0005 0.0167 0.0004 0.0016 0.0018
None 0.1525 0.1159 0.09 0.0015 0.1003 0.0564 0.0294 0.0797 0.0283 0.0433 0.0659 0.014 0.043 0.092 0.013 0.0157 0.0058 0.0261
Nuclear Periphery 0.0336 0.0181 0.0207 0.0307 0.0488 0.0222 0.0336 0.0145 0.0214 0.0208 0.0442 0.0075 0.0381 0.0167 0.0163 0.012 0.0263 0.011
Nucleolus 0.0124 0.0144 0.0189 0.0084 0.0077 0.0325 0.0115 0.0178 0.0179 0.0036 0.0074 0.0197 0.0135 0.0122 0.0152 0.0074 0.0185 0.0264
Nucleus 0.5806 0.6535 0.763 0.8542 0.3748 0.6969 0.6553 0.7239 0.764 0.7612 0.6669 0.8365 0.6471 0.7499 0.8086 0.8911 0.7745 0.7848
Peroxisomes 0.0118 0.0009 0.0024 0.0004 0.0302 0.0008 0.0012 0.0057 0.0055 0.0122 0.0015 0.0131 0.0028 0.0001 0.0031 0 0.0006 0.0064
Punctate Nuclear 0.0823 0.1068 0.0356 0.0087 0.0665 0.1043 0.0606 0.0717 0.0437 0.0156 0.0614 0.0515 0.0671 0.0514 0.07 0.052 0.0566 0.0869
Vacuole 0.0016 0.0064 0.0017 0.0017 0.0105 0.0008 0.0043 0.0013 0.0015 0.0005 0.0048 0.0006 0.0028 0.0014 0.0035 0.0004 0.0171 0.0006
Vacuole Periphery 0.0014 0.0006 0.0005 0.0025 0.0101 0.0006 0.0022 0.0002 0.001 0.0003 0.0026 0.0004 0.0017 0.0003 0.0012 0.0001 0.0037 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0309 5.2677 6.189 6.6176 11.7785 4.4171 7.7673 5.542 6.8803 11.7743
Translational Efficiency 0.4759 1.0179 0.3896 0.4142 0.4596 1.0949 0.5411 0.476 0.4253 0.3791

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘