Standard name
Description Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.09 0.05 0.06 0.08 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.94 0.92 0.84 0.78 0.77 0.78 0.76 0.79 0.98 1.0 0.97 0.98 0.97 0.97 0.96 0.98 0.92 0.96 0.97 0.87
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.12 0.17 0.09 0.12 0.12 0.07 0 0 0 0 0 0.08 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.1 0.1 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 9 18 31 19 4 5 0 0 0 0 0 0 0 0 1 0 0 1
Bud 2 2 0 0 0 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 2 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 8 4 5 9 14 11 27 31 23 6 8 1 2 7 11 11 9 1 0 1 0 0 0
Cytoplasm 236 297 409 215 244 329 277 407 268 361 74 81 171 226 243 331 237 142 228 284 378 140 218 152
Endoplasmic Reticulum 2 4 1 2 11 21 38 91 31 54 12 7 3 2 4 9 10 11 2 0 11 2 2 10
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Golgi 1 0 1 0 0 0 5 4 5 3 2 1 0 0 0 1 2 0 0 0 1 0 0 0
Mitochondria 0 0 1 0 2 2 11 12 39 44 10 14 0 0 0 3 0 3 1 0 1 0 0 0
Nucleus 1 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 0 1 3 1 3 0 0 1 0 1 1 4 0 0 1 2 0 0 3
Unique Cell Count 239 302 413 219 259 357 329 520 346 463 98 103 174 227 250 338 244 146 238 289 410 146 226 174
Labelled Cell Count 250 312 416 224 266 369 353 565 408 507 108 117 176 230 256 357 264 165 238 289 410 146 226 174


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.2 50.2 43.8 43.0 36.8 32.7 28.1 26.8 25.9 23.5 22.3 23.1 45.5 45.5 42.0 47.6 56.5 57.6 42.9 42.5 43.8
Std Deviation (1e-4) 11.0 9.4 10.1 10.3 7.3 5.5 6.6 5.9 5.4 4.6 5.1 5.0 8.4 9.3 9.4 15.1 14.8 16.9 9.1 9.8 11.5
Intensity Change (Log2) – – – -0.03 -0.25 -0.42 -0.64 -0.71 -0.76 -0.9 -0.97 -0.92 0.05 0.05 -0.06 0.12 0.37 0.39 -0.03 -0.04 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.8 6.2 4.2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.3 2.7 2.3 3.6 5.2 3.4 0 0 0 0 0 2.2 2.9 0
Cytoplasm -0.9 -3.7 -4.8 -7.6 -9.5 -9.5 -9.5 -9.2 -8.5 -0.8 0.7 -1.8 -1.3 -1.8 -1.5
Endoplasmic Reticulum 0 0 4.7 6.9 8.8 6.0 7.0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.3 2.7 6.8 6.2 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0