Standard name
Human Ortholog
Description Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.09 0.05 0.06 0.08 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.94 0.92 0.84 0.78 0.77 0.78 0.76 0.79 0.98 1.0 0.97 0.98 0.97 0.97 0.96 0.98 0.92 0.96 0.97 0.87
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.12 0.17 0.09 0.12 0.12 0.07 0 0 0 0 0 0.08 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.1 0.1 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 9 18 31 19 4 5 0 0 0 0 0 0 0 0 1 0 0 1
Bud 2 2 0 0 0 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 2 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7 8 4 5 9 14 11 27 31 23 6 8 1 2 7 11 11 9 1 0 1 0 0 0
Cytoplasm 236 297 409 215 244 329 277 407 268 361 74 81 171 226 243 331 237 142 228 284 378 140 218 152
Endoplasmic Reticulum 2 4 1 2 11 21 38 91 31 54 12 7 3 2 4 9 10 11 2 0 11 2 2 10
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Golgi 1 0 1 0 0 0 5 4 5 3 2 1 0 0 0 1 2 0 0 0 1 0 0 0
Mitochondria 0 0 1 0 2 2 11 12 39 44 10 14 0 0 0 3 0 3 1 0 1 0 0 0
Nucleus 1 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 0 0 1 3 1 3 0 0 1 0 1 1 4 0 0 1 2 0 0 3
Unique Cell Count 239 302 413 219 259 357 329 520 346 463 98 103 174 227 250 338 244 146 238 289 410 146 226 174
Labelled Cell Count 250 312 416 224 266 369 353 565 408 507 108 117 176 230 256 357 264 165 238 289 410 146 226 174


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.2 50.2 43.8 43.0 36.8 32.7 28.1 26.8 25.9 23.5 22.3 23.1 45.5 45.5 42.0 47.6 56.5 57.6 42.9 42.5 43.8
Std Deviation (1e-4) 11.0 9.4 10.1 10.3 7.3 5.5 6.6 5.9 5.4 4.6 5.1 5.0 8.4 9.3 9.4 15.1 14.8 16.9 9.1 9.8 11.5
Intensity Change (Log2) -0.03 -0.25 -0.42 -0.64 -0.71 -0.76 -0.9 -0.97 -0.92 0.05 0.05 -0.06 0.12 0.37 0.39 -0.03 -0.04 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.8 6.2 4.2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.3 2.7 2.3 3.6 5.2 3.4 0 0 0 0 0 2.2 2.9 0
Cytoplasm -0.9 -3.7 -4.8 -7.6 -9.5 -9.5 -9.5 -9.2 -8.5 -0.8 0.7 -1.8 -1.3 -1.8 -1.5
Endoplasmic Reticulum 0 0 4.7 6.9 8.8 6.0 7.0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.3 2.7 6.8 6.2 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.1197 55.1441 48.9217 48.0838 40.8137 46.4275 42.6646 55.1773 50.3688 45.279 47.4635 48.0526 42.9618 58.3571 51.5024 47.4635 53.899 51.0337
Actin 0.0385 0.0051 0.0033 0.0001 0.0038 0.0009 0.024 0.0002 0.0016 0.0002 0.0043 0.0006 0.012 0.0001 0.0117 0.0014 0.0002 0.0022
Bud 0.001 0.0003 0.0011 0.0009 0.001 0.0008 0.0011 0.0002 0.0004 0.0011 0.0006 0.0003 0.0008 0.0002 0.0006 0.0016 0.0002 0.0009
Bud Neck 0.0034 0.0008 0.0016 0.001 0.0018 0.0035 0.0005 0.0002 0.0003 0.0003 0.0008 0.0006 0.0005 0.0002 0.0004 0.0005 0.0005 0.0027
Bud Periphery 0.0014 0.0001 0.0014 0.0009 0.0019 0.0009 0.0011 0.0002 0.0005 0.0008 0.0005 0.0002 0.0006 0.0002 0.0003 0.001 0 0.0017
Bud Site 0.0025 0.0033 0.0053 0.0002 0.0009 0.0004 0.0025 0.0003 0.0003 0.0001 0.0007 0 0.0018 0.0001 0.0007 0.0028 0 0.0001
Cell Periphery 0.0028 0.0013 0.008 0.003 0.0007 0.0011 0.001 0.0003 0.0003 0.0005 0.0001 0.0001 0.0007 0.0002 0.0001 0.0011 0.0001 0.0005
Cytoplasm 0.7276 0.9598 0.8994 0.7522 0.8274 0.8731 0.8376 0.9806 0.9421 0.9395 0.9203 0.9471 0.8248 0.9836 0.9557 0.9048 0.9372 0.9626
Cytoplasmic Foci 0.0077 0.0007 0.0015 0.0011 0.0025 0.0031 0.0038 0.001 0.0087 0.0046 0.0061 0.0033 0.0113 0.0005 0.0023 0.0122 0.0024 0.0025
Eisosomes 0.0006 0.0001 0.0007 0 0.0001 0 0.0005 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0448 0.0115 0.0076 0.0062 0.0223 0.02 0.018 0.0036 0.0118 0.0011 0.0085 0.0096 0.043 0.0042 0.0031 0.0032 0.0445 0.0095
Endosome 0.017 0.0013 0.0044 0.0039 0.0222 0.0088 0.0163 0.0023 0.006 0.0091 0.0092 0.0094 0.03 0.002 0.0081 0.0173 0.0021 0.0043
Golgi 0.0094 0.0002 0.0011 0.0001 0.0064 0.0015 0.0034 0.0003 0.0018 0.0002 0.0066 0.0015 0.0054 0.0001 0.0019 0.0018 0.0002 0.0008
Lipid Particles 0.0078 0.0001 0.0006 0.0001 0.0014 0.0072 0.0017 0.0001 0.003 0.0001 0.0002 0.0001 0.0041 0 0.0003 0.0034 0 0.0001
Mitochondria 0.0037 0.0001 0.0114 0.0001 0.0498 0.0217 0.014 0.0041 0.0011 0.0003 0.0325 0.0182 0.0059 0.0002 0.0007 0.0065 0.0002 0.0035
None 0.0086 0.0001 0.0003 0.0001 0.0002 0.0003 0.0033 0.0003 0.0017 0.0003 0.0002 0.0006 0.0045 0.0001 0.0005 0.0032 0.0001 0.0003
Nuclear Periphery 0.0294 0.0031 0.0076 0.0075 0.0037 0.0079 0.0137 0.0005 0.0015 0.0014 0.0013 0.0013 0.0199 0.0017 0.0026 0.0019 0.0064 0.0024
Nucleolus 0.0013 0 0.0002 0.0001 0.0002 0.0004 0.0101 0.0003 0.0001 0.0001 0 0 0.0007 0 0 0.0007 0 0
Nucleus 0.0099 0.0018 0.0039 0.0045 0.0023 0.0034 0.0081 0.0014 0.001 0.0019 0.0007 0.001 0.0084 0.0011 0.0026 0.0023 0.0013 0.0018
Peroxisomes 0.0015 0 0.0004 0 0.0004 0.0011 0.0005 0 0.0026 0 0.0012 0 0.0003 0 0.0003 0.0005 0 0.0001
Punctate Nuclear 0.0043 0.0001 0.0002 0 0.0001 0.0001 0.0053 0 0.0003 0 0 0 0.0025 0 0.0002 0.0003 0.0001 0.0001
Vacuole 0.0635 0.0094 0.0362 0.1826 0.0409 0.0337 0.0277 0.003 0.0112 0.0318 0.0043 0.0045 0.0185 0.0046 0.0063 0.0268 0.0037 0.0029
Vacuole Periphery 0.0134 0.0008 0.004 0.0353 0.0101 0.01 0.0056 0.0011 0.0035 0.0066 0.0018 0.0014 0.0041 0.0006 0.0015 0.0066 0.0007 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 199.7021 193.5485 191.3465 199.3497 190.4867 155.2379 188.7075 186.0224 189.1726 196.6498
Translational Efficiency 1.5239 1.6155 1.5023 1.5284 1.4159 1.7078 1.5388 1.2521 1.2929 1.2679

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1255 1230 198 2112 1481 1816 654 2677 2736 3046 852 4789

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2574.39 3152.25 3619.03 4219.53 2972.65 3202.06 3683.41 4238.13 2789.97 3181.95 3668.45 4229.93
Standard Deviation 455.11 696.29 486.50 867.05 559.34 804.29 548.66 888.93 551.13 762.92 535.55 879.40
Intensity Change Log 2 0.292151 0.491372 0.712852 0.107251 0.309292 0.511678 0.196017 0.396660 0.608540

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000098 0.000636 0.000226 0.000066 0.000095 0.000148 0.000075 0.000060 0.000096 0.000262 0.000142
Bud Neck 0.000166 0.000426 0.010092 0.000993 0.000303 0.000343 0.001731 0.000933 0.000240 0.000377 0.003674 0.000959
Bud Site 0.001686 0.002973 0.013502 0.008735 0.001736 0.005691 0.003146 0.007642 0.001713 0.004594 0.005553 0.008124
Cell Periphery 0.002300 0.004331 0.027157 0.015134 0.004752 0.002980 0.007027 0.001759 0.003627 0.003525 0.011705 0.007657
Cytoplasm 0.931325* 0.892239* 0.531976* 0.849315* 0.882051* 0.927722* 0.831090* 0.808271* 0.904653* 0.913394* 0.761578* 0.826372*
Cytoplasmic Foci 0.006007 0.005470 0.039734 0.001989 0.005767 0.006102 0.007332 0.003356 0.005877 0.005847 0.014862 0.002754
Eisosomes 0.000001 0.000001 0.000014 0.000003 0.000001 0.000001 0.000002 0.000001 0.000001 0.000001 0.000005 0.000002
Endoplasmic Reticulum 0.000434 0.000590 0.000729 0.000176 0.000640 0.000360 0.000175 0.000122 0.000545 0.000453 0.000304 0.000146
Endosome 0.000483 0.000665 0.003374 0.000625 0.000724 0.000528 0.000549 0.000591 0.000614 0.000583 0.001206 0.000606
Golgi 0.023854 0.023201 0.052089 0.003580 0.038799 0.016104 0.014275 0.002305 0.031944 0.018970 0.023063 0.002867
Lipid Particles 0.004263 0.004654 0.031940 0.010783 0.007427 0.003606 0.010800 0.002031 0.005976 0.004029 0.015713 0.005890
Mitochondria 0.000221 0.000129 0.000146 0.000169 0.000061 0.000216 0.000062 0.000163 0.000134 0.000181 0.000082 0.000166
Mitotic Spindle 0.000394 0.000427 0.005807 0.000204 0.000701 0.000776 0.000977 0.001178 0.000560 0.000635 0.002099 0.000748
None 0.001329 0.002604 0.001252 0.000875 0.001258 0.003192 0.001181 0.001203 0.001291 0.002955 0.001197 0.001059
Nuclear Periphery 0.000057 0.000206 0.001826 0.000084 0.000148 0.000085 0.000360 0.000035 0.000106 0.000134 0.000701 0.000056
Nuclear Periphery Foci 0.004334 0.006949 0.020145 0.001744 0.009564 0.004166 0.006759 0.000693 0.007165 0.005290 0.009870 0.001157
Nucleolus 0.000029 0.000206 0.000129 0.000127 0.000021 0.000051 0.000063 0.000254 0.000025 0.000113 0.000079 0.000198
Nucleus 0.003105 0.012013 0.041706 0.007597 0.005231 0.006943 0.024196 0.011484 0.004256 0.008990 0.028265 0.009770
Peroxisomes 0.000013 0.000047 0.000054 0.000114 0.000011 0.000024 0.000012 0.000167 0.000012 0.000033 0.000022 0.000143
Vacuole 0.010573 0.027224 0.185805 0.091935 0.025241 0.014185 0.080778 0.147106 0.018513 0.019450 0.105186 0.122775
Vacuole Periphery 0.009374 0.015546 0.031888 0.005592 0.015499 0.006831 0.009335 0.010632 0.012689 0.010350 0.014576 0.008409

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.49 -7.35 -4.29 -3.05 4.79 -2.88 -6.73 -1.78 0.49 4.40 -3.87 -9.60 -4.65 -2.55 4.70
Bud Neck -4.90 -9.22 -5.02 -3.45 8.42 -1.22 -7.36 -9.18 -8.27 4.19 -4.08 -11.17 -9.29 -7.49 8.66
Bud Site -3.05 -4.21 -8.10 -6.47 1.63 -5.16 -4.08 -9.67 -1.97 -5.31 -5.86 -5.56 -12.47 -5.11 -2.43
Cell Periphery -3.67 -10.38 -13.15 -10.11 4.75 3.02 -2.49 5.84 4.18 7.35 0.20 -9.06 -7.33 -7.86 4.44
Cytoplasm 5.77 17.95 13.72 6.69 -14.09 -6.99 7.15 11.79 22.29 0.76 -1.60 16.03 17.75 20.88 -7.74
Cytoplasmic Foci 0.61 -8.06 6.49 6.99 8.82 -0.78 -3.84 3.83 4.13 5.79 -0.16 -8.22 7.17 7.32 10.11
Eisosomes -2.30 -8.64 -6.21 -5.01 7.08 -2.13 -6.84 -2.71 -1.76 2.91 -3.17 -10.12 -6.39 -4.86 6.42
Endoplasmic Reticulum -0.96 -1.14 2.82 3.00 2.78 2.39 4.40 4.94 4.54 2.32 0.98 3.19 5.67 4.83 3.19
Endosome -1.29 -6.34 -0.56 0.11 5.24 2.12 1.09 1.57 -0.67 0.38 0.26 -5.12 -0.02 -0.16 3.84
Golgi 0.22 -5.03 9.99 11.97 9.99 8.89 9.73 15.80 12.73 11.37 7.15 4.68 18.58 17.35 13.70
Lipid Particles -0.50 -9.00 -6.32 -5.96 6.71 4.18 -2.88 6.08 2.74 7.49 3.05 -7.94 -0.29 -3.90 8.04
Mitochondria 0.53 0.30 0.24 -1.35 -0.30 -2.99 -0.70 -4.13 0.83 -4.18 -0.64 0.52 -0.56 0.25 -4.25
Mitotic Spindle -0.53 -5.31 2.41 3.31 5.45 -0.54 -1.40 -1.22 -1.05 -0.55 -0.89 -5.39 -0.97 -0.67 3.54
None -4.25 0.35 2.64 5.90 2.39 -3.59 0.80 0.22 3.38 -0.15 -4.82 1.01 1.36 5.12 0.72
Nuclear Periphery -3.27 -10.14 -3.45 2.53 9.96 3.81 -6.62 7.02 3.91 9.62 -1.43 -11.58 4.41 3.56 12.35
Nuclear Periphery Foci -3.09 -7.92 5.77 6.61 9.40 7.08 2.92 13.96 7.65 10.88 3.25 -3.76 14.90 9.88 13.49
Nucleolus -1.98 -7.66 -5.97 0.86 1.01 -3.66 -11.42 -19.08 -13.49 -14.38 -2.48 -9.50 -17.25 -2.37 -11.71
Nucleus -9.24 -9.50 -9.38 2.82 8.23 -2.99 -11.03 -11.19 -8.98 5.55 -8.98 -14.63 -14.27 -5.36 9.93
Peroxisomes -2.21 -4.40 -9.57 -3.54 -3.84 -3.54 -1.47 -11.57 -10.27 -11.34 -3.26 -4.21 -14.88 -9.90 -12.97
Vacuole -6.83 -12.80 -22.92 -16.32 6.66 4.66 -10.74 -27.56 -31.67 -7.37 -0.83 -16.34 -35.46 -35.07 -1.22
Vacuole Periphery -3.35 -3.96 3.34 6.38 4.86 6.69 3.95 4.46 -3.65 -0.66 2.21 -0.39 5.46 3.10 3.63