Standard name
Description Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.99 0.98 0.99 0.96 0.89 0.85 0.84 0.83 0.86 0.84 0.97 1.0 0.99 0.8 0.85 0.68 0.27 0.64 0.42 0.31 0.37 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 0 0 0.05 0 0 0 0 0.05 0 0 0.08 0 0.07 0.09 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0 0 0 0.05 0.05 0.24 0.22 0.23 0.2 0.23 0 0 0 0.27 0.21 0.42 0.55 0.25 0.46 0.44 0.46 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 3 3
Bud 0 0 0 2 2 5 4 2 2 2 1 2 1 1 0 0 0 0 3 0 4 7 4
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 2 0 2 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 162 276 164 293 326 203 426 324 336 288 341 212 450 496 123 28 52 49 180 70 59 138 141
Endoplasmic Reticulum 1 0 0 0 0 0 1 0 0 0 0 2 0 0 5 1 3 1 1 1 5 9 6
Endosome 2 0 1 0 8 5 16 3 0 4 0 2 0 0 3 0 2 8 6 1 14 8 8
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 2
Mitochondria 5 4 0 1 0 22 7 17 11 18 18 2 1 0 7 1 2 15 9 12 16 15 21
Nucleus 0 0 1 0 0 3 1 10 14 4 5 0 0 0 2 2 1 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 2 1 0 0 0 1 0 1 0 0 0 0 1 0 2
Nucleolus 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 7 0 1 1 2 1 2 0 0 0 0 0 0 1 1 0 0 7 4
Vac/Vac Membrane 21 4 3 3 17 12 120 85 92 65 95 1 0 1 42 7 32 100 69 77 83 172 143
Unique Cell Count 177 279 167 297 338 227 500 387 404 333 406 218 452 499 153 33 77 182 281 168 192 374 347
Labelled Cell Count 192 286 169 301 364 250 579 444 460 384 463 222 455 500 182 40 92 182 281 168 192 374 347


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.1 4.9 5.2 5.6 4.2 5.8 5.3 5.1 4.9 4.8 4.6 4.5 4.7 5.2 5.2 5.9 4.5 4.4 4.5
Std Deviation (1e-4) 0.9 0.8 0.8 2.1 1.1 1.2 1.6 2.7 1.2 0.9 0.9 1.1 1.2 1.8 1.2 1.0 1.8 1.9 1.6 1.5
Intensity Change (Log2) 0.08 0.21 -0.21 0.24 0.12 0.05 0.0 -0.03 -0.1 -0.11 -0.06 0.09 0.09 0.27 -0.11 -0.14 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 6.6 8.2